Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,142,702	C→T	A83T (GCG→ACG)	udk ←	uridine/cytidine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,142,702	0	C	T	100.0%	103.8 / NA	31	A83T (GCG→ACG)	udk	uridine/cytidine kinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/19);  total (12/19)

CACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCTGTAAACCGGCAGGTCAATTGCCGAGCCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGATCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGATGGCTTTGATCTTTGTAATAGCAGTCTTCGGGAATTACGCCGATGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATC  >  NC_000913/2142457‑2142942                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 cACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCTGTAAACCGGCAGGTTAATTGCCGAGCCGCGTTTCAACGTTTGt                                                                                                                                                                                                                                              <  1:313975/250‑1 (MQ=255)                                                                       ggggTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAACCGCGCATCCGTCCGCAACAAAATGCCTTCGAGAATGATGACCGTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCTGTAAACCGGCAGGTCAATTGCCGAGCCGCGTTTCAACGTTTGTAAATGCTCAAGCAGCAGACTGTCATCCATCGCGCTGGGATGGTAGTAGTTGGTCTTAACGCGTTCTTCCAt                                                                                                                                                                       >  2:29721/1‑251 (MQ=255)                                                                       ggggTATCAACGAAAATGGAGAAGGTAAGTGCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCTGTAAACCGGCAGGTCAATTGCCGAGCCGCGTTTCAACGTTTGTAAATGCTCAAGCAGCAGACTGTGATCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCAt                                                                                                                                                                       <  1:164287/251‑1 (MQ=255)                                                                                    aaaTGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCTGTGAACCGGCAGGTCAATTGCCGAGCCGCGTTTCAACGTTTGTAAATGCTCAAGCAGCAGACTGTGATCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGATGGCtt                                                                                                                                                          <  2:263137/251‑1 (MQ=255)                                                                                     aaTGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCTGTAAACCGGCAGGTCAATTGCCGAGCCGCGTTTCAACGTTTGTAAATGCTCAAGCAGCAGACTGTGATCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGATGGCttt                                                                                                                                                         <  2:298512/251‑1 (MQ=255)                                                                                        ggAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCTGTAAACCGGCAGGTCAATTGCCGAGCCGCGTTTCAACGTTTGTAAATGCTCAAGCAGCAGACTGTGATCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGATGGCTTTGAt                                                                                                                                                      <  1:351203/251‑1 (MQ=255)                                                                                              gttaagttCATCACGCAAACGCGCAACCGTCAGCAACAAAATGCCTGCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCTGTAAACCGGCAGGTCAATTGCCGAGCCGCGTTTCAACGTTTGTAAATGCTCAAGCAGCAGACTGTGATCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCTACAGATGGCTTTGATCTTTGt                                                                                                                                                <  2:266118/251‑1 (MQ=255)                                                                                                 aagttCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCTGTAAACCGGCAGGTCAATTGCCGAGCCGCGTTTCAACGTTTGTAAATGCTCAAGCAGCAGACTGTGATCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGATGGCTTTGATCTTTGTaa                                                                                                                                              <  1:265075/250‑1 (MQ=255)                                                                                                            gCAAACGCTCATCCGACAGCAACATAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCTTTTCTTTCATACGCGTATGTTCAACATAGCTGTAAACCGGCAGGTCAATTGCCGAGCCGCGTTTCAACGTTTGTAAGTGCTCAAGCAGCAGACTGTGATCCATCGCGCTGGGAGGGTCGTAGTTGGTCGTAACTCGGTCTTCCATCGACAGATGGCTTTGATCTTTGTAATAGCAGTCTTCg                                                                                                                                  <  1:356995/251‑1 (MQ=255)                                                                                                             cAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCTGTAAACCGGCAGGTCAATTGCCGAGCCGCGTTTCAACGTTTGTAAATGCTCAAGCAGCAGACTGTGATCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGATGGCTTTGATCTTTGTAATAGCAGTCTTCgg                                                                                                                                 >  2:87490/1‑251 (MQ=255)                                                                                                                    gcATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCTGTAAACCGGCAGGTCAATTGCCGAGCCGCGTTTCAACGTTTGTAAATGCTCAAGCAGCAGACTGTGATCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGATGGCTTTGATCTTTGTAATAGCAGTCTTCGGGAATTAc                                                                                                                          <  1:95162/251‑1 (MQ=255)                                                                                                                       tCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCTGTAAACCGGCAGGTCAATTGCCGATCCGCGTTTCAACGTTTGTAAATGCTCAAGCAGCAGACTGTGATCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGATGGCTTTGATCTTTGTAATAGCAGTCTTCGGGAATTACGcc                                                                                                                       >  2:74700/1‑251 (MQ=255)                                                                                                                                     aaTGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCTGTAAACCGGCAGGTCAATTGCCGAGCCGCGTTTCAACGTTTGTAAATGCTCAAGCAGCAGACTGTGATCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGATGGCTTTGATCTTTGTAATAGCAGTCTTCGGGAATTACGCCGATGTGTTCATCAc                                                                                                         <  1:312809/251‑1 (MQ=255)                                                                                                                                      aTGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCTGTAAACCGGCAGGTCAATTGCCGAGCCGCGTTTCAACGTTTGTAAATGCTCAAGCAGCAGACTGTGATCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGATGGCTTTGATCTTTGTAATAGCAGTCTTCGGGAATTACGCCGATGTGTTCATCAcc                                                                                                        <  2:196773/251‑1 (MQ=255)                                                                                                                                           ttCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCTGTAAACCGGCAGGTCAATTGCCGAGCCGCGTTTCAACGTTTGTAAATGCTCAAGCTGCAGACTGTGATCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGATGGc                                                                                                                                                            >  2:255878/1‑194 (MQ=255)                                                                                                                                           ttCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCTGTAAACCGGCAGGTCAATTGCCGAGCCGCGTTTCAACGTTTGTAAATGCTCAAGCAGCAGACTGTGATCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGATGGc                                                                                                                                                            <  1:255878/194‑1 (MQ=255)                                                                                                                                                     atgaCCTTCTTCGACTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCTGTAAACCGGCAGGTCAATTGCCGAGCCGCGTTTCAACGTTTGTAAATGCTCAAGCACCAGACTGTGCTCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGATGGCTTTGATCTTTGTAATAGCAGTCTTCGGGAATTACGCCGATGAGTTCATCACCGACTTGCTCACGCa                                                                                          >  2:212373/1‑250 (MQ=255)                                                                                                                                                            tcttcGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCTGTAAACCGGCAGGTCAATTGCCGAGCCGCGTTTCAACGTTTGTAAATGCTCAAGCAGCAGACTGTGATCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGATGGCTTTGATCTTTGTAATAGCAGTCTTCGGGAATTACGCCGATGTGTTCATCACCGACTTGCTCa                                                                                              >  1:251664/1‑239 (MQ=255)                                                                                                                                                            tcttcGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCTGTAAACCGGCAGGTCAATTGCCGAGCCGCGTTTCAACGTTTGTAAATGCTCAAGCAGCAGACAGTGATCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGATGGCTTTGATCTTTGTAATAGCAGTCTTCGGGAATTACGCCGATGTGTTCATCACCGACTTGCTCa                                                                                              <  2:251664/239‑1 (MQ=255)                                                                                                                                                               tcGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCTGTAAACCGGCAGGTCAATTGCCGAGCCGCGTTTCAACGTTTGTAAATGCTCAAGCAGCAGACTGTGATCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGATGGCTTTGATCTTTGTAATAGCAGTCTTCGGGAATTACGCCGATGTGTTCATCACCGACTTGCTCACGCAATTCACGAt                                                                                 <  2:65173/249‑1 (MQ=255)                                                                                                                                                                                  ttctttcATACGCGTATGTTCAACATAGCTGTAAACCGGCAGGTCAATTGCCGAGCCGCGTTTCAACGTTTGTAAATGCTCAAGCAGCAGACTGTGATCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGATGGCTTTGATCTTTGTAATAGCAGTCTTCGGGAATTACGCCGATGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTgg                                                                     <  2:195940/242‑1 (MQ=255)                                                                                                                                                                                  ttctttcATACGCGTATGTTCAACATAGCTGTAAACCGGCAGGTCAATTGCCGAGCCGCGTTTCAACGTTTGTAAATGCTCAAGCAGCAGACTGTGATCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGATGGCTTTGATCTTTGTAATAGCAGTCTTCGGGAATTACGCCGATGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTgg                                                                     >  1:195940/1‑242 (MQ=255)                                                                                                                                                                                             gcgTATGTTCAACATAGCTGTAAACCGGCAGGTCAATTGCCGAGCCGCGTTTCAACGTTTGTAAATGCTCAAGCAGCAGACTGTGATCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGATGGCTTTGATCTTTGTAATAGCAGTCTTCGGGAATTACGCCGATGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGa                                                  >  2:131865/1‑250 (MQ=255)                                                                                                                                                                                                        aCATAGCTGTAAACCGGCAGGTCAATTGCCGAGCCGCGTTTCAACGTTTGTAAATGCTCAAGCAGCAGACTGTGATCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGATGGCTTTGATCTTTGTAATAGCAGTCTTCGGGAATTACGCCGATGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGcc                                      >  1:79834/1‑251 (MQ=255)                                                                                                                                                                                                          aTAGCTGTAAACCGGCAGGTCAATTGCCGAGCCGCGTTTCAACGTTTGTAAATGCTCAAGCAGCAGACTGTGATCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGATGGCTTTGATCTTTGTAATAGCAGTCTTCGGGAATTACGCCGATGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAg                                    <  1:87490/251‑1 (MQ=255)                                                                                                                                                                                                                     cctgCAGGTCAAGTGCCGAGTCGCGTTTCAACGTTTGTAAATGCTCAAGCAGCAGACTGTGATCCTGCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGATGGCTTTGATCTTTGTAATAGCAGTCTTCGGGAATTACGCCGATGTGTTCATCACCGACTTGCTCACGCAATTCACGAGAAAGGGTACTGGCAATAAGACTCTTCTCGGAAGCCGATGCGCCAGCGATACCGaaa                         <  2:121947/248‑3 (MQ=255)                                                                                                                                                                                                                       ggCAGGTCAATTGCCGAGCCGCGTTTCAACGTTTGTAAATGCTCAAGCAGCAGACTGTGATCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGATGGCTTTGATCTTTGTAATAGCAGTCTTCGGGAATTACGCCGATGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAAt                       >  1:15411/1‑251 (MQ=255)                                                                                                                                                                                                                           ggTCAATTGCCGAGCCGCGTTTCAACGTTTGTAAATGCTCAAGCAGCAGACTGTGATCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGATGGCTTTGATCTTTGTAATAGCAGTCTTCGGGAATTACGCCGATGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACg                   <  1:280252/251‑1 (MQ=255)                                                                                                                                                                                                                                          cgcgTTTCAACGTTTGTAAATGCTCAAGCAGCAGACTGAGATCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGATGGCTTTGATCTTTGTAATAGCAGTCTTCGGGAATTACGCCGATGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTg     >  2:260603/1‑250 (MQ=255)                                                                                                                                                                                                                                            cgTTTCAACGTTTGTAAATGCTCAAGCAGCAGACTGTGATCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGATGGCTTTGATCTTTGTAATAGCAGTCTTCGGGAATTACGCCGATGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATATTGACGCACTGATGAGACTGaac  <  2:240404/251‑3 (MQ=255)                                                                                                                                                                                                                                            cgTTTCAACGTTTGTAAATGCTCAAGCAGCAGACTGTGATCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGATGGCTTTGATCTTTGTAATAGCAGTCTTCGGGAATTACGCCGATGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATc  >  2:200258/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 CACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCTGTAAACCGGCAGGTCAATTGCCGAGCCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGATCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGATGGCTTTGATCTTTGTAATAGCAGTCTTCGGGAATTACGCCGATGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATC  >  NC_000913/2142457‑2142942

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑