Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,004,516	T→C	G215G (GGT→GGC)	fliD →	flagellar filament capping protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,004,516	0	T	C	100.0%	170.4 / NA	52	G215G (GGT→GGC)	fliD	flagellar filament capping protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/27);  total (25/27)

CGACAAAGATCCGATTACCATTGATATCAGCGCGGCTAACTCATCGTTAAGCGGGATCCGTGATGCCATCAACAACGCAAAAGCAGGCGTAAGCGCAAGCATCATTAACGTGGGTAACGGTGAATATCGTCTGTCAGTCACATCAAATGACACCGGCCTTGATAATGCGATGACACTCTCGGTCAGCGGTGATGATGCGCTACAAAGTTTTATGGGCTATGACGCCAGTGCCAGCAGCAACGGTATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCTTCAGCAGCCTGACCAAATACACCG  >  NC_000913/2004273‑2004760                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     tgataaaGATCCGATTACCTTTGATATCAGCGCGGCTAACTCATCGTTAAGCGGGATCCGTGATGCCATCAACAACACAACAGCAGGCGTAAGCGCAAGCATCATTAACGTGGGTAACGGTGAATATCGTCTGTCAGTCACATCAAATGACACCGGCCTTGATAAAGCGATGACACCCTCGGGCAGAGGTGATGATGCGCTACAAAGTTTTATGGGCTATGACGCCAGTGCCTGCAGCAACGTCATGGAgg                                                                                                                                                                                                                                               >  2:179507/5‑251 (MQ=255)       aGATCCGATTACCATTGATATCAGCGCGGCTAACTCATCGTTAAGCGGGATCCGTGATGCCATCAACAACGCAAAAGCAGGCGTAAGCGCAAGCATCATTAACGTGGGTAACGGTGAATATCGTCTGTCAGTCACATCAAATGACACCGGCCTTGATAATGCGATGACACTCTCGGTCAGCGGTGATGATGCGCTACAAAGTTTTATGGGCTATGACGCCCGTGCCAGCAGCAACGGCATGGAGGTCTCgg                                                                                                                                                                                                                                         >  2:273658/1‑251 (MQ=255)         aTCCGATTACCATTGATATCAGCGCGGCTAACTCATCGTTAAGCGGGATCCGTGATGCCATCAACAACGCAAAAGCAGGCGTAAGCGCAAGCATCATTAACGTGGGTAACGGTGAATATCGTCTGTCAGTCACATCAAATGACACCGGCCTTGATAATGCGATGACACTCTCGGTCAGCGGTGATGATGCGCTACAAAGTTTTATGGGCTATGACGCCAGTGCCAGCAGCAACGGCATGGAGGTCTCGGtt                                                                                                                                                                                                                                       <  2:1221/251‑1 (MQ=255)          tCCGATTGCCATTGATATCAGCGCGGCTAACTCATCGTTAAGCGGGATCCGTGATGCCATCAACAACGCAAAAGCAGGCGTAAGCGCAAGCATCATTAACGTGGGTAACGGTGAATATCGTCTGTCAGTCACATCAAATGACACCGGCCTTGATAATGCGATGACACTCTCGGTCAGCGGTGATGATGCGCTACAAAGTTTTATGGGCTATGACGCCAGTGCCAGCAGCAACGGCATGGAGGTCTCGGTTg                                                                                                                                                                                                                                      >  1:51989/1‑251 (MQ=255)                   cATTGATATCAGCGCGGCTAACTCATCGTTAAGCGGGATCCGTGATGCCATCAACAACGCAAAAGCAGGCGTAAGCGCAAGCATCATTAACGTGGGTAACGATGAATATCGTCTGTCAGTCACATCAAATGACACCGGCCTTGATAATGCGATGACACTCTCGGTCAGCGGTGATGATGCGCTACAAAGTTTTATGGGCTATGACGCCAGTGCCAGCAGCAACGTCATGGAGGTCTCGGTTGCCGccc                                                                                                                                                                                                                                >  1:61743/1‑248 (MQ=255)                             aGCGCGGCTAACTCATCGTTAAGCGGGATCCGTGATGCCATCAACAACGCAAAAGCAGGCGTAAGCGCAAGCATCATTAACGTGGGTAACGGTGAATATCGTCTGTCAGTCACATCAAATGACACCGGCCTTGATAATGCGATGACACTCTCGGTCAGCGGTGATGATGCGCTACAAAGTTTTATGGGCTATGACGCCAGTGCCAGCAGCAACGGCATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCt                                                                                                                                                                                                                   <  2:63326/251‑1 (MQ=255)                                gcgGCTAACTCACAGTTCAGCGGGATCCGTGATGCCCTCACCAACGCAAAAACAGGCGTAAGCGCAAGCATCATTAACGTGGGTAACGGTGAATATCGTCTGTCAGTCACATCAAATGACACCGGCCTTGATAATGCGATGACACTCTAGGTCAGCGGTGATGATGCGCTACAAAGTTTTATGGGCTATGACGCCAGTGCCAGCAGCAACGGCATATAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGAc                                                                                                                                                                                                                >  2:208731/1‑251 (MQ=255)                                 cgGCTAACTCATCGTTAAGCGGGATCCGTGATGCCATCAACAACGCAAAAGCAGGCGTAAGCGCAAGCATCATTAACGTGGGTAACGGTGAATATCGTCTGTCAGTCACATCAAATGACACCGGCCTTGATAATGCGATGACACTCTCGGTCAGCGGTGATGATGCGCTACAAAGTTTTATGGGCTATGACGCCAGTGCCAGCAGCAACGGCATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACa                                                                                                                                                                                                               <  2:267334/251‑1 (MQ=255)                                          cATCGTTAAGCGGGATCCGTGATGCCATCAACAACGCAAAAGCAGGCGTAAGCGCAAGCATCATTAACGTGGGTAACGGTGAATATCGTCTGTCAGTCACATCAAATGACACCGGCCTTGATAATGCGATGACACTCTCGGTCAGCGGTGATGATGCGCTACAAAGTTTTATGGGCTATGACGCCAGTGCCAGCAGCAACGGCATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTcaacaa                                                                                                                                                                                                       <  1:208444/250‑1 (MQ=255)                                          cATCGTTAAGCGGGATCCGTGATGCCATCAACAACGCAAAAGCAGGCGTAAGCGCAAGCATCATTAACGTGGGTAACGGTGAATATCGTCTGTCAGTCACATCAAATGACACCGGCCTTGATAATGCGATGACACTCTCGGTCAGCGGTGATGATGCGCTACAAAGTTTTATGGGCTATGACGCCAGTGCCAGCAGCAACGGCATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTcaacaa                                                                                                                                                                                                       <  1:208433/250‑1 (MQ=255)                                             cGTTAAGCGGGATCCGTGATGCCATCAACAACGCAAAAGCAGGCGTAAGCGCAAGCATCATTAACGTGGGTAACGGTGAATATCGTCTGTCAGTCACATCAAATGACACCGGCCTTGATAATGCGATGACACTCTCGGTCAGCGGTGATGATGCGCTACAAAGTTGTAGGGGCTATGACGCCAGTGCCAGCAGCAACGGCATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGt                                                                                                                                                                                                    <  1:20169/250‑1 (MQ=255)                                                    cGGGATCCGTGATGCCATCAACAACGCAAAAGCAGGCGTAAGCGCAAGCATCATTAACGTGGGTAACGGTGAATATCGTCTGTCAGTCACATCAAATGACACCGGCCTTGATAATGCGATGACACTCTCGGTCAGCGGTGATGATGCGCTACAAAGTTTTATGGGCTATGACGCCAGTGCCAGCAGCAACGGCATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCg                                                                                                                                                                                            >  1:25626/1‑251 (MQ=255)                                                              gATGCCATCAACAACGCAAAAGCAGGCGTAAGCGCAAGCATCATTAACGTGGGTAACGGTGAATATCGTCTGTCAGTCACATCAAATGACACCGGCCTTGATAATGCGATGACACTCTCGGTCAGCGGTGATGATGCGCTACAAAGTTTTATGGGCTATGACGCCAGTGCCAGCAGCAACGGCATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAAcagca                                                                                                                                                                                   <  1:62448/250‑1 (MQ=255)                                                                tGCCATCCACAACGCAACAGCAGGCGTAAGCGCAAGCATCATTAACGTGGGTAACGGTGAACCTCGTATGCCAGTCACATCAAATGACACCCGTCTTGCTACTGCGATGACACTCTCGGTCAGCGGCGATGATGCGCTCCAAAGTTTTATGGGCTATGACGCCAGTGCCAGAAGCACCCGCATCAAGGTCTCGGTTGCCGCCCAGAATGCGCGGTTGACAGGCaacaactgacccctcgcgacacccagc                                                                                                                                                                                 >  2:345169/1‑229 (MQ=255)                                                                         aaacGCAAAAGCAGGCGTAAGCGCAAGCATCATTAACGTGGGTAACGGTGAATATCGTCTGTCAGTCACATCAAATGACACCGGCCTTGATAATGCGATGACACTCTCGGTCAGCGGTGATGATGCGCTACAAAGTTTTATGGGCTATGACGCCAGTGCCAGCAGCAACGGCATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCa                                                                                                                                                                       <  2:315620/250‑1 (MQ=255)                                                                            cGCAAAAGCAGGCGTAAGCGCAAGCATCATTAACGTGGGTAACGGTGAATATCGTCTGTCAGTCACATCAAATGACACCGGCCTTGATAATGCGATGACACTCTCGGTCAGCGGTGATGATGCGCTACAAAGTTTTATGGGCTATGACGCCAGTGCCAGCAGCAACGGCATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACTTCGCCATCGAGAACAGCAGCAACACCATCAGCg                                                                                                                                                                    <  1:332879/251‑1 (MQ=255)                                                                               aaaaGCAGGCGTAAGCGCAAGCATCATTAACGTGGGTAACGGTGAATATCGTCTGTCAGTCACATCAAATGACACCGGCCTTGATAATGCGATGACACGCTCGGTCAGCGGTGATGATGCGCTACAAAGTTTTATGGGCTATGACGCCAGTGCCAGCAGCAACGGCATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGAcg                                                                                                                                                                 <  2:214589/251‑1 (MQ=255)                                                                                    cAGGCGTAAGCGCAAGCATCATTAACGTGGGTAACGGTGAATATCGTCTGTCAGTCACATCAAATGACACCGGCCTTGATAATGCGATGACACTCTCGGTCAGCGGTGATGATGCGCTACAAAGTTTTATGGGCTATGACGCCAGTGCCAGCAGCAACGGCATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTg                                                                                                                                                            >  2:166369/1‑251 (MQ=255)                                                                                    cAGGCGTAAGCGCAAGCATAATTAACGTGGGTAACGGTGAATATCGTCTGTCAGTCACATCAAATGACACCGGCCTTGATAATGCGATGACACTCTCGGTCAGCGGTGATGATGCGCTACAAAGTTTTATGGGCTATGACGCCAGTGCCAGCAGCAACGGCATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGAGACGCGCtc                                                                                                                                                            >  1:101590/1‑250 (MQ=255)                                                                                           aaGCGCAAGCATCATTAACGTGGGTAACGGTGAATATCGTCTGTCAGTCACATCAAATGACACCGGCCTTGATAATGCGATGACACTCTCGGTCAGCGGTGATGATGCGCTACAAAGTTTTATGGGCTATGACGCCAGTGCCAGCAGCAACGGCATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAAc                                                                                                                                                      >  1:273235/1‑250 (MQ=255)                                                                                                cAAGCATCATTAACGTGGGTAACGGAGAATATCGTCTGTCAGTCACATCAAATGACACCGGCCTTGATAATGCGAAGACACTCTCGGTCAGCGGTGATGATGCGCTACAAAGTTTTATGGGCTATGACGCAAGCGCCAGCAGCAACGGCATGGAGGTCTCGGTTGCCGCCCAGAATGAGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCAcc                                                                                                                                                >  1:318906/1‑251 (MQ=255)                                                                                                               tGGGTAACGGTGAATATCGTCTGTCAGTCACATCAAATGACACCGGCCTTGATAATGCGATGACACTCTCGGTCAGCGGTGATGATGCGCTACAAAGTTTTATGGGCTATGACGCCAGTGCCAGCAGCAACGGCATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGAt                                                                                                                                 >  1:370956/1‑251 (MQ=255)                                                                                                                 ggTAACGGTGAATATCGTCTGTCAGTCACATCAAATGACACCGGCCTTGATAATGCGATGACACTCTCGGTCAGCGGTGATGATGCGCTACAAAGTTTTATGGGCTATGACGCCAGTGCCAGCAGCAACGGCATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGt                                                                                                                               >  2:128048/1‑251 (MQ=255)                                                                                                                                cGTCTGTCAGTCACATCAAATGACACCGGCCTTGATAATGCGATGACACTCTCGGTCAGCGGTGATGATGCGCTACAAAGTTTTATGGGCTATGACGCCAGTGCCAGCAGCAACGGCATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAAcc                                                                                                                 >  1:248495/1‑250 (MQ=255)                                                                                                                                    tGTCAGTCACATCAAATGACACCGGCCTTGATAATGCGATGACACTCTCGGTCAGCGGTGATGATGCGCTACAAAGTTTTATGGGCTATGACGCCAGTGCCAGCAGCAACGGCATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACg                                                                                                            >  1:333597/1‑251 (MQ=255)                                                                                                                                         gtcaCATCAAATGACACCGGCCTTGATAATGCGAAGACACGCTCGGTCAGCGGTGATGATGCGCTACAAAGTTTTATGGGCTATGACGCCAGTGCCAGCAGCAACGGCATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAAc                                                                                                       <  2:91555/251‑1 (MQ=255)                                                                                                                                            acaTCAAATGACACCGGCCTTGATAATGCGATGACACTCTCGGTCAGCGGTGATGATGCGCTACAAAGTTTTATGGGCTATGACGCCAGTGCCAGCAGCAACGGCATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCAt                                                                                                    <  2:51989/251‑1 (MQ=255)                                                                                                                                                   aTGACACCGGCCTTGATAATGCGATGACACTCTCGGTCAGCGGTGATGATGCGCTACAAAGTTTTATGGGCTATGACGCCAGTGCCAGCAGCAACGGCATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAg                                                                                             <  2:306475/251‑1 (MQ=255)                                                                                                                                                      acacCGGCCTTGATAATGCGATGACACTCTCGGTCAGCGGTGATGCTGCGCTACAAAGTTTTATGGGCTATGACGCCAGTGCCAGCAGCAACGGCATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGACAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGac                                                                                          >  1:207714/1‑251 (MQ=255)                                                                                                                                                       cacCGGCCTTGATAATGCGATGACACTCTCGGTCAGCGGTGATGATGCGCTACAAAGTTTTATGGGCTATGACGCCAGTGCCAGCAGCAACGGCATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGaca                                                                                         <  1:220265/251‑1 (MQ=255)                                                                                                                                                        acCGGCCTTGATAATGCGATGACACTCTCGGTCAGCGGTGATGATGCGCTACAAAGTTTTATGGGCTATGACGCCAGTGCCAGCAGCAACGGCATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGacac                                                                                        <  1:25793/251‑1 (MQ=255)                                                                                                                                                          cGGCCTTGATAATGCGATGACACTCTCGGTCAGCGGTGATGATGCGCTACAAAGTTTTATGGGCTATGACGCCAGTGCCAGCAGCAACGGCATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCt                                                                                      >  1:88174/1‑251 (MQ=255)                                                                                                                                                           ggCCTTGATAATGCGATGACACTCTCGGTCAGCGGTGATGATGCGCTACAAAGGTTTATGGGCTATGACGCCAGTGCCAGCAGCAACGGCATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGATGCTAACCATCACTCAGGACACCTc                                                                                     <  2:213042/251‑1 (MQ=255)                                                                                                                                                                            gACACTCTCGGTCAGCGGTGATGATGCGCTACAAAGTGTTATGGGCTATGACGCCAGTGCCAGCAGCAACGGCATGGATGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAGCATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAAACATCACTCAGGACACCTCCAAAGCGCAAACGGCg                                                                     <  2:65929/250‑1 (MQ=255)                                                                                                                                                                             acacTCTCGGTCAGCGGTGATGATGCGCTACAAAGTTTTATGGGCTATGACGCCAGTGCCAGCAGCAACGGCATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAgcgc                                                                             >  2:135641/1‑241 (MQ=255)                                                                                                                                                                             acacTCTCGGTCAGCGGTGATGATGCGCTACAAAGTTTTATGGGCTATGACGCCAGTGCCAGCAGCAACGGCATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAgcgc                                                                             <  1:135641/241‑1 (MQ=255)                                                                                                                                                                                     ggTCAGCGGTGATGATGCGCTACAAAGTTTTATGGGCTATGACGCCAGTGCCAGCAGCAACGGCATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGGCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACt                                                           <  1:257591/251‑1 (MQ=255)                                                                                                                                                                                      gTCAGCGGTGATGATGCGCTACAAAGTTTTATGGGCTATGACGCCAGTGCCAGCAGCAACGGCATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTg                                                          <  2:271844/251‑1 (MQ=255)                                                                                                                                                                                        cAGCGGTGATGATGCGCTACAAAGTTTTATGGGCTATGACGCCAGTGCCAGCAGCAACGGCATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTggg                                                        >  2:324232/1‑251 (MQ=255)                                                                                                                                                                                          gcggtgattaggtgcgaCAAAGTGTTATGGTCTAAGACGCCAATGCCAGCAGCAACGGCATGGAGGTCTCGTGTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACGCCAGCAGCGACGCGCGGGAAAACATCACCCTGAACCTGAACGATATCACCACGGTCAACCAGACGCTAACCATCACTCAGGACACCGCCAATGGGCAAACGCCGATTAAAGattcggtg                                                      <  2:160694/235‑8 (MQ=255)                                                                                                                                                                                              tgatgaTGCGCTACAAAGTTTTATGGGCTATGACGCCAGTGCCAGCAGCAACGGCATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATg                                                  >  1:289148/1‑251 (MQ=255)                                                                                                                                                                                                   atgCGCTACAAAGTTTTATGGGCTATGACGCCAGTGCCAGCAGCAACGGCATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTAc                                             >  2:160172/1‑251 (MQ=255)                                                                                                                                                                                                     gcgcTACAAAGTGTTATGGGCTATGACGCCAGTGCCAGCAGCAACGGCATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCGAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACa                                            <  2:61743/250‑1 (MQ=255)                                                                                                                                                                                                           cAAAGTTTTATGGGCTATGACGCCAGTGCCAGCAGCAACGGCATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCt                                     <  2:316865/251‑1 (MQ=255)                                                                                                                                                                                                                 tttATGGGCTATGACGCCAGTGCCAGCAGCAACGGCATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCTCTCAGGACACCGCCAAAGCGCAAACGTCGATTAAAGACTGGGTGAATGCCTTCATCTCGGTAATAg                                <  2:54681/250‑1 (MQ=255)                                                                                                                                                                                                                       ggCTATGACGCCAGTGCCAGCAGCAACGGCATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCtt                         >  2:338456/1‑251 (MQ=255)                                                                                                                                                                                                                           aTGACGCCAGTGCCAGCAGCAACGGCATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCTTca                       <  2:248495/249‑1 (MQ=255)                                                                                                                                                                                                                           aTGACGCCAGTGCCAGCAGCAACGGCATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATGAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCTTca                       <  1:211161/249‑1 (MQ=255)                                                                                                                                                                                                                                       ccAGCAGCAACGGCATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCTTCAGCAGCCTGACCaa          >  1:212799/1‑250 (MQ=255)                                                                                                                                                                                                                                         agcagcAACGGCATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCTTCAGCAGCCTGACCAAAt        >  2:279649/1‑250 (MQ=255)                                                                                                                                                                                                                                             gcaACGGCATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCTTCAGCAGCCTGACCAAATACAcc   <  1:261142/251‑1 (MQ=255)                                                                                                                                                                                                                                              caACGGCATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCTTCAGCAGCCTGACCAAATACAccg  >  1:325605/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     CGACAAAGATCCGATTACCATTGATATCAGCGCGGCTAACTCATCGTTAAGCGGGATCCGTGATGCCATCAACAACGCAAAAGCAGGCGTAAGCGCAAGCATCATTAACGTGGGTAACGGTGAATATCGTCTGTCAGTCACATCAAATGACACCGGCCTTGATAATGCGATGACACTCTCGGTCAGCGGTGATGATGCGCTACAAAGTTTTATGGGCTATGACGCCAGTGCCAGCAGCAACGGTATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCTTCAGCAGCCTGACCAAATACACCG  >  NC_000913/2004273‑2004760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑