Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,952,609	T→C	V115A (GTG→GCG)	yecN →	MAPEG family inner membrane protein YecN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,952,609	0	T	C	100.0%	83.5 / NA	27	V115A (GTG→GCG)	yecN	MAPEG family inner membrane protein YecN
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/13);  total (14/13)

GCGAACTGCAAAGCGCTATTCGCATTCATGGTAACGCGGTGGAATATATTCCCATCGCGATTGTGCTGATGCTGTTTATGGAAATGAATGGCGCAGAAACCTGGATGGTGCATATTTGCGGCATCGTTTTGCTTGCTGGTCGTCTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCAGCGTTCCGTACCCGGCTATTCCAATATTATTTCCATGATTGGTAT  >  NC_000913/1952378‑1952844                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                            gggAACTGAAAAGCGCTATTCGCATTCAGGGTAACGCGGTGGAATATATTCCCATCGCGATTGTGCTGATGCTGTTTATGGAAATGAATGGCTCAGAAACCTGGATGGTGTATATTTGCGGCATCGTTTTGTTTGCTGGTTGTCTGATGCATTATTACGGTTTTCATCTCCGTCTGTTCCGCTTGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGCGCTGGCGAATCTTTGGtat                                                                                                                                                                                                                          <  2:39623/249‑1 (MQ=255)                              ggTAACGCGGTGGAATATATTCCCATCGCGATTGTGCTGATGCTGTTTATGGAAATGAATGGCGCAGAAACCTGGATGGTGCATATTTGCGGCATCGTTTTGCTTGCTGGTCGTCTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGCGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCg                                                                                                                                                                                             >  2:292647/1‑251 (MQ=255)                                        tGGAATATATTCCCATCGCGATTGTGCTGATGCTGTTTATGGAAATGAATGGCGCAGAAACCTGGATGGTGCATATTTGCGGCATCGTTTTGCTTGCTGGTCGTCTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGCGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGcac                                                                                                                                                                                    >  2:92289/1‑250 (MQ=255)                                           aaTATATTCCTATCGAGATAGTGATTACGCTGTCTATCGAAATGAATGGCCAACATACCTGGATGGAGCATATTTACGACATCGTTTCGCTTGCTAGAAGTCTGATGCAATATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACATCCCGGCCTCACCGCCACCTGGTGTGCGCTGTTGCTGAAGGCGCTGGAGAATCTTTGGTCTATGCCCTGGGCGCTGTTTTTATCCCTGCGTTAgcccaaaat                                                                                                                                                                                 >  2:156143/1‑243 (MQ=255)                                              atatTCCCATCGCGATTGTGCTGATGCTGTTTATGGAAATGAATGGCGCAGAAACCTGGATGGTGCATATTTGCGGCATCGTTTTGCTTGCTGGTCGTCTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGCGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACg                                                                                                                                                                              <  2:278254/250‑1 (MQ=255)                                              atatTCCCATCGCGATTGTGCTGATGCTGTTTATGGAAATGAATGGCGCAGAAACCTGGATGGTGCATATTTGCGGCATCGTTTTGCTTGCTGGTCGTCTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGCGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACg                                                                                                                                                                              <  1:119345/250‑1 (MQ=255)                                              atatTCCCATCGCGATTGTGCTGATGCTGTTTATGGAAATGAATGGCGCAGAAACCTGGATGGTGCATATTTGCGGCATCGTTTTGCTTGCTGGTCGTCTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGCGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGc                                                                                                                                                                             >  2:334998/1‑251 (MQ=255)                                                 ttCCCATCGCGATTGTGCTGATGCTGTTTATGGAAATGAATGGCGCAGAAACCTGGATGGTGCATATTTGCGGCATCGTTTTGCTTGCTGGTCGTCTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGCGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCAc                                                                                                                                                                          >  1:5547/1‑251 (MQ=255)                                                         gcgATTGTGCTGATGCTGTTTATGGAAATGAATGGCGCAGAAACCTGGATGGTTCATATTTGCGGCATCGTTTTGCTTGCTGGTCGTCTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGCGCTGGCGAATCTTTGGGATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCtttt                                                                                                                                                                  <  1:59781/251‑1 (MQ=255)                                                         gcgATTGTGCTGATGCTGTTTATGGAAATGAATGGCGCAGAAACCTGGATGGTGCATATTTGCGGCATCGTTTTGCTTGCTGGTCGTCTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGCGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCtttt                                                                                                                                                                  <  1:292647/251‑1 (MQ=255)                                                                          gTTTATGGAAATGAATGGCGCAGAAACCTGGATGGTGCATATTTGCGGCATCGTTTTGCTTGCTGGTCGTCTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGCGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACg                                                                                                                                                   <  1:334998/249‑1 (MQ=255)                                                                                                       ggATGGTGCATATTTGCGGCATCGTTTTGCTTGCTGGTCGTCTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGCGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCt                                                                                                                      <  2:242628/249‑1 (MQ=255)                                                                                                       ggATGGTGCATATTTGCGGCATCGTTTTGCTTGCTGGTCGTCTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGCGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGc                                                                                                                    >  2:323332/1‑251 (MQ=255)                                                                                                                          cATCGTTTTGCTTGCTGGTCGTCTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGCGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGc                                                                                                 <  1:239479/251‑1 (MQ=255)                                                                                                                                       gctGGTCGTCTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGCGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCttt                                                                                     <  2:154209/250‑1 (MQ=255)                                                                                                                                              gtcTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGCGCTGGCGAATCTTTGGTATATGCCCTgg                                                                                                                                                                                                                 >  2:50739/1‑119 (MQ=255)                                                                                                                                              gtcTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGCGCTGGCGAATCTTTGGTATATGCCCTgg                                                                                                                                                                                                                 <  1:50739/119‑1 (MQ=255)                                                                                                                                                      cATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCAGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGCGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCA‑AATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTg                                                                     >  1:310203/1‑250 (MQ=255)                                                                                                                                                              cGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGCGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTc                                                             >  1:343433/1‑251 (MQ=255)                                                                                                                                                                 ttttCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCTTCAGCGCCACATGGTGTGCGCTGTTGCTGATGGCGCTGGCGAAACTTTGGCATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCCCTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGCCACGCTATTTTCTTCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCg                                                          >  1:24319/1‑251 (MQ=255)                                                                                                                                                                    tcatcaCCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGCGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGat                                                       >  1:178773/1‑251 (MQ=255)                                                                                                                                                                      atcaCCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGCGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCGTCCCGGatat                                                     <  1:92289/251‑1 (MQ=255)                                                                                                                                                                            gTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGCGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATcc                                                >  1:120013/1‑250 (MQ=255)                                                                                                                                                                                 ttCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGCGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCAGCGtt                                          >  1:206162/1‑251 (MQ=255)                                                                                                                                                                                                                       tgCGCTGATGCTGATGGCGCTGGCGAATCTTTGGTATATGCCCTGGGCGATGGTTTTCTCACTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCAGCGTTCCGTACCCGGCTATTCCAATATTATTTCCATGATTGGt    >  1:372956/1‑251 (MQ=255)                                                                                                                                                                                                                        tcgcTGTTGCTGATTGCGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACGTTCTTTTTCCCGGATTTTTACGTTATGGCTCTCCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTGTGATGAACGGGTAGCTTAAGTCTTCCCGGATATGATCCAGCGTTCCGTACCCGGCTATTCCAATATTAGTTCCATGATTGGt    <  2:243086/249‑1 (MQ=255)                                                                                                                                                                                                                         cgcTGTTGCTGATGGCGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCAGCGTTCCGTACCCGGCTATTCCAATATTATTTCCATGATTGGTAt  <  1:156317/251‑1 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                            GCGAACTGCAAAGCGCTATTCGCATTCATGGTAACGCGGTGGAATATATTCCCATCGCGATTGTGCTGATGCTGTTTATGGAAATGAATGGCGCAGAAACCTGGATGGTGCATATTTGCGGCATCGTTTTGCTTGCTGGTCGTCTGATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATCCAGCGTTCCGTACCCGGCTATTCCAATATTATTTCCATGATTGGTAT  >  NC_000913/1952378‑1952844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑