Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,848,366	C→T	R104H (CGC→CAC)	ydjA ←	putative oxidoreductase YdjA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,848,366	0	C	T	100.0%	110.9 / NA	34	R104H (CGC→CAC)	ydjA	putative oxidoreductase YdjA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/17);  total (17/17)

CAGAAATAAGTTACAAACGGCGTCGGGTCCGGGACGTTAATCGACGTAGATGCTTTCAGCTGCGGCGTACCGAGGTAGAGAAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGGCAGCAATCGCCCCCTGTTCCAGTACGGCGCTGAAACGCTCGCGCCCTTCCCCTTCAATCACAAAAAAATGCCACGGTTGCATGGACTTATGGTCCGGCGCACGCATACCCGCACGCAGGATGTTTTGCAGTTGTTCACCCG  >  NC_000913/1848126‑1848612                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                       cAGAAATAAGTTACAAACGGCGTCGGGTCCGGGACGTTAATCGACGTAGATGCTTTCAGCTGCGGCGTACCGAGGTAGAGAAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGTGCGGGACttt                                                                                                                                                                                                                                              >  2:171142/1‑251 (MQ=255)      aTAAGTTACAAACGGCGTCGGGTCCGGGACGTTAATCGACGTAGATGCTTTCAGCTGCGGCGTACCGAGGTAGCGAAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGTGCGGGACTTTATGAt                                                                                                                                                                                                                                         >  1:207742/1‑251 (MQ=255)      aTAAGTTACAAACGGCGTCGGGTCCGGGACGTTAATCGACGTAGATGCTTTCAGCTGCGGCGTACCGAGGTAGAGAAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGTGCGGGACTTTATGAt                                                                                                                                                                                                                                         <  1:215204/251‑1 (MQ=255)                aaCGGCGTCGGGTCCGGGACGTTAATCGACGTAGATGCTTTCAGCTGCGGCGTACCGAGGTAGAGAAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTTCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGTGCGGGACTTTATGATTCTCTTCGCa                                                                                                                                                                                                                               <  2:121813/251‑1 (MQ=255)                    gCGTCGGGTCCGGGACGTTAATCGACGTAGATGCTTTCAGCTGCGGCGTACCGAGGTAGAGAAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGTGCGGGACTTTATGATtct                                                                                                                                                                                                                                      >  1:331669/1‑240 (MQ=255)                    gCGTCGGGTCCGGGACGTTAATCGACGTAGATGCTTTCAGCTGCGGCGTACCGAGGTAGAGAAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTACCTGTTCCCAGTGCGGGACTTTATGATtct                                                                                                                                                                                                                                      <  2:331669/240‑1 (MQ=255)                          ggTCCGGGACGTTAATCGACGTAGATGCTTTCAGCTGCGGCGTACCGAGGTAGAGAAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGTGCGGGACTTTATGATTCTCTTCGCATTTCGccacc                                                                                                                                                                                                                     >  2:46269/1‑251 (MQ=255)                             ccGGGACGTTAATCGACGTAGATGCTGTCAGCTGCGGCGTACCGAGGTAGAGAAAACCGACAATTTTATCCGGCTCACGGCAACCGAATGCTTCACGCACTACCGGACGTTCAGTTAATGCGCAACTGCGCCAGATGCCGCCAAACCCCGGGGCAACTGCTGCCAGTTGCATCGCCATGACCGCGCATCCGGCAGACATTGCCTGTTCCCAGTGCGGGACTTTATGATTCTCTTCGCATTTCGCCaccacc                                                                                                                                                                                                                  <  2:236914/251‑1 (MQ=255)                                 gACGTTCATCGACGTAGATACTTTCAGCTGCGGCGTCCCGAGGTAGAGAAAACCGACACTTTTATCCTGCTCACGGCACCCGCATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCCCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGTGCGGGCCTTTATGATTCTCTTCGCATTTCGCCACCACCGtg                                                                                                                                                                                                               >  2:221845/1‑250 (MQ=255)                                               tAGATGCTTTCAGCTGCGGCGTACCGAGGTAGAGAAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGTGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTgcg                                                                                                                                                                                                >  2:45098/1‑251 (MQ=255)                                                       ttCAGCTGCGGCGTACAGAGGTAGAGAAAACCGACAATTTTATCCTGATCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGTGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACgg                                                                                                                                                                                        >  2:215631/1‑251 (MQ=255)                                                          aGCTGCGGCGTACCGAGGTAGAGAAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCACACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGCCATTTCCTGTTCCCAGTGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGgcgc                                                                                                                                                                                     >  1:167833/1‑251 (MQ=255)                                                                ggcgTACCGAGGTAGAGAAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGTGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACg                                                                                                                                                                               <  1:46269/251‑1 (MQ=255)                                                                     aCCGAGGTAGAGAAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGTGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCAGTGCGCGGAACGGCGCATTACGGGCtt                                                                                                                                                                          <  1:49344/251‑1 (MQ=255)                                                                                    aCCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGCTGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCAGGCAGACATTTACTGTTCCCAGTGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGCTGAGCGGTGCGCGGACCGGCGCATTACGGGCTTTGTCGATAGCTTTGt                                                                                                                                                           >  2:234624/1‑251 (MQ=255)                                                                                                         aCGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGTGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGGCAGCAATCg                                                                                                                                      <  1:326672/251‑1 (MQ=255)                                                                                                                        ttCACGCAATACCGGCAATTCAGTTAATGCGCCACTGAGCCAGATGCCGCCACACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGTGCGGGACTTTATGCTTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATCGCTTTGTCATCACTACCGGCCGCAATCGCCCCCTGCTCCAGt                                                                                                                        >  2:183055/1‑250 (MQ=255)                                                                                                                                 tACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGTGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGGCAGCAATCGCCCCCTGTTCCAGTACGGCGCTg                                                                                                               >  1:35794/1‑250 (MQ=255)                                                                                                                                              gTTAATGCGCCACTGCGCCAGATGCAGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGTGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGGCAGCAATCGCCCCCTGTTCCAGTACGGCGCTGAAACGCTCGCGccc                                                                                                 >  1:77792/1‑251 (MQ=255)                                                                                                                                                       ccACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGTGCGGGACTTTATGATTCTCTTCGCATTTCGCCATCACCGtgatga                                                                                                                                                                                                           >  2:255072/1‑136 (MQ=255)                                                                                                                                                       ccACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGTGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGtgatga                                                                                                                                                                                                           <  1:255072/136‑1 (MQ=255)                                                                                                                                                              gcCAGCTGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGTGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGGCAGCAATCGCCCCCTGTTCCAGTACGGCGCTGAAACGCTCGCGCCCTTCCCCTTCAATcac                                                                                  >  1:247837/1‑250 (MQ=255)                                                                                                                                                                   aTGCCGCCAAACCCCTGGGCAACTTCTGCCAGTTGCATCGCCATGACCGCGCATCCGGCAGACATTGCCTGTTCCCAGTGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGGCAGCAATCGCCCCCTGTTCCAGTACGGCGCTGAAACGCTTGCGCCCTTCCCCTTCAATCACaaaaaa                                                                            <  2:207742/251‑1 (MQ=255)                                                                                                                                                                                 cTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGTGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGTTAGCTTTGTCATCACTACCGGCAGCAATCGCCCCCTGTTCCAGTACGGCGCTGAAACGCTCGCGCCCTTCCCCTTCAATCACAAAAAAATGCCACGGTTGc                                                               >  2:355252/1‑250 (MQ=255)                                                                                                                                                                                       aacggctgcCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGTGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGGCAGCAATCGCCCCCTGTTCCAGTACGGCGCTGAAACGCTCGCGCCCTTCCCCTTCAATCACAAAAAAATGCCACGGTTGCATGGACt                                                        <  1:354371/247‑1 (MQ=255)                                                                                                                                                                                        aCTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGTGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGGCAGCAATCGCCCCCTGTTCCAGTACGGCGCTGAAACGCTCGCGCCCTTCCCCTTCAATCACAAAAAAATGCCACGGTTGCATGGACtt                                                       >  1:337220/1‑251 (MQ=255)                                                                                                                                                                                           gctgcCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGTGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGGCAGCAATCGCCCCCTGTTCCAGTACGGCGCTGAAACGCTCGCGCCCTTCCCCTTCAATCACAAAAAAATGCCACGGTTGCATGGACTTATg                                                    <  1:226124/251‑1 (MQ=255)                                                                                                                                                                                              gcCATTTGCAGCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGTGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGGCAGCAATCGCCCCCTGTTCCAGTACGGCGCTGAAACGCTCGCGCCCTTCCCCTTTAATCACAAAAAAATGCCACGGTTGCATGGACTTATGGt                                                  <  2:241982/250‑1 (MQ=255)                                                                                                                                                                                                   ttGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGTGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGGCAGCAATCGCCCCCTGTTCCAGTACGGCGCTGAAACGCTCGCGCCCTTCCCCTTCAATCACAAAAAAATGCCACGGTTGCATGGACTTATGGTCCGgc                                             <  1:268752/250‑1 (MQ=255)                                                                                                                                                                                                         cGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGTGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGGCAGCAATCGCCCCCTGTTCCAGTACGGCGCTGAAACGCTCGCGCCCTTCCCCTTCAATCACAAAAAAATGCCACGGTTGCATGGACTTATGGTCCGGcgcacgca                                      <  1:244841/251‑1 (MQ=255)                                                                                                                                                                                                                aCCGCGCATCCGGCAGACATTTCCTGTTCCCAGTGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGGCAGCAATCGCCCCCTGTTCCAGTACGGCGCTGAAACGCTCGCGCCCTTCCCCTTCAATCACAAAAAAATGCCACGGTTGCATGGACTTATGGTCCGGCGCACGCATACCcgc                               <  1:171142/251‑1 (MQ=255)                                                                                                                                                                                                                   gcgcATCCGGCAGACATTTCCTGTTCCCAGTGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGGGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGGCAGCAATCGCCCCCTGTTCCAGTACGGCGCTGAAACGCTCGCGCCCTTCCCCTGCAATCACAAAAAAATGCCACGGTTGCATGGACTTATGGTCCGGCGCACGCATACCcgcacg                            <  1:215631/251‑1 (MQ=255)                                                                                                                                                                                                                                            cccAGTGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGGCAGCAATCGCCCCCTGTTCCAGTACGGCGCTGAAACGCTCGCGCCCTTCCCCTTCAATCACAAAAAAATGCCACGGTTGCATGGACTTATGGTCCGGCGCACGCATACCCGCACGCAGGATGTTTTGCAGTTGTTCAccc   <  1:165984/251‑1 (MQ=255)                                                                                                                                                                                                                                             ccAGTGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGGCAGCAATCGCCCCCTGTTCCAGTACGGCGCTGAAACGCTCGCGCCCTTCCCCTTCAATCACAAAAAAATGCCACGGTTGCATGGACTTATGGTCCGGCGCACGCATACCCGCACGCAGGATGTTTTGCAGTTGTTCACCCg  >  2:285106/1‑251 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                       CAGAAATAAGTTACAAACGGCGTCGGGTCCGGGACGTTAATCGACGTAGATGCTTTCAGCTGCGGCGTACCGAGGTAGAGAAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGGCAGCAATCGCCCCCTGTTCCAGTACGGCGCTGAAACGCTCGCGCCCTTCCCCTTCAATCACAAAAAAATGCCACGGTTGCATGGACTTATGGTCCGGCGCACGCATACCCGCACGCAGGATGTTTTGCAGTTGTTCACCCG  >  NC_000913/1848126‑1848612

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑