Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,734,226	T→C	intergenic (‑125/‑209)	grxD ← / → mepH	glutaredoxin 4/peptidoglycan endopeptidase/peptidoglycan L,D‑carboxypeptidase MepH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,734,226	0	T	C	100.0%	92.3 / NA	27	intergenic (‑125/‑209)	grxD/mepH	glutaredoxin 4/peptidoglycan endopeptidase/peptidoglycan L,D‑carboxypeptidase MepH
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/15);  total (12/15)

AAGCGCCTGGACTGCCTGGGCAGAGAAACCGCAGCTCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTTCGATAGTGGTGCTCATTGTCTTGCTTCCTCAAACTTCTTTACGGCAGTAATCTGACATTGTAGCGGGTCAGTGCGGCATCGGAAAATAACATTTTCATCACGCTTTTGCTATTTTATCCCTTTGCTCTATTTGTTGCATTTCAAATATTCGTTTCTTCTCTGATGCCGGCAAACTGGCGTATTTATAACCATTTTTAGCTTTCACTGCTGCATTTTTTTGCGCTCGCCAACGAAACGTATTTTTTAACAATAAAAGCTATTAACTTTCTCTTCTTCTATGCATTAGAATCATCAAGTTTTGTAAATCAGACGCAGGCATGATAGACCTGCCTTTACAGAGGGACGCTCAGTGGCGCGGATAAACCGTATTTCGATCACGCTCTGTG  >  NC_000913/1733982‑1734471                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      aaGCGCCTGGACTGCCTGGGCAGAGAAACCGCAGCTCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTTCGATAGTGGTGCTCATTGTCTTGCTTCCTCAAACTTCTTTACGGCAGTAATCTGACATTGTAGCGGGTCAGTGCGGCATCGGAAAATAACATTTTCATCACGCTTTTGCTATTTTATCCCTTTGCTCTATTTGTTGCATTCCAAata                                                                                                                                                                                                                                                 <  2:149757/251‑1 (MQ=255)              gCCTGGGCAGAGAAACCGCAGCTCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTTCGATAGTGGTGCTCATTGTCTTGCTTCCTCAAACTTCTTTACGGCAGTAATCTGACATTGTAGCGGGTCAGTGCGGCATCGGAAAATAACATTTTCATCACGCTTTTGCTATTTTATCCCTTTGCTCTATTTGTTGCATTCCAAATATTCGTttcttc                                                                                                                                                                                                                                      <  1:375839/249‑1 (MQ=255)              gCCTGGGCAGAGAAACCGCAGCTCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTTCGATAGTGGTGCTCATTGTCTTGCTTCCTCAAACTTCTTTACGGCAGTAATCTGACATTGTAGCGGGTCAGTGCGGCATCGGAAAATAACATTTTCATCACGCTTTTGCTATTTTATCCCTTTGCTCTATTTGTTGCATTCCAAATATTCGTTTCTtctc                                                                                                                                                                                                                                    >  2:143657/1‑251 (MQ=255)                                                     ccTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTTCGATAGTGGTGCTCATTGTCTTGCTTCCTCAAACTTCTTTACGGCAGTAATCTGACATTGTAGCGGGTCAGTGCGGCATCGGAAAATAACATTTTCATCACGCTTTTGCTATTTTATCCCTTTGCTCTATTTGTTGCCTTCCAAATATTCGTTTCTTCTCTGATGCCGGCAAACTGGCGTATTTATAACCATTTTTAGc                                                                                                                                                                                             >  2:17737/1‑251 (MQ=255)                                                                           ggTTTTCAGCAATCTGGCGTTGGATTTTTTCGATAGTGGTGCTCATTGTCTTGCTTCCTCAAACTTCTTTACGGCAGTAATCTGACATTGTAGCGGGTCAGTGCGGCATCGGAAAATAACATTTTCATCACGCTTTTGCTATTTTATCCCTTTGCTCTATTTGTTGCATTCCAAATATTCGTTTCTTCTCTGATGCCGGCAAACTGGCGTATTTATAACCATTTTTAGCTTTCACTGCTGCATTTTTTTgc                                                                                                                                                                       <  1:93863/251‑1 (MQ=255)                                                                                 cAGCAATCTGGCGTTGGATTTTTTCGATAGTGGTGCTCATTGTCTTGCTTCCTCAAACTTCTTTACGGCAGTAATCTGACATTGTAGCGGGTCAGTGCGGCATCGGAAAATAACATTTTCATCACGCTTTTGCTATTTTATCCCTTTGCTCTATTTGTTGCATTCCAAATATTCGTTTCTTCTCTGATGCCGGCAAACTGGCGTATTTATAACCATTTTTAGCTTTCACTGCTGCATTTTTTTGCGCTCg                                                                                                                                                                  <  2:225322/250‑1 (MQ=255)                                                                                    cAATCTGGCGTTGGATTTTTTCGATAGTGGTGCTCATTGTCTTGCTTCCTCAAACTTCTTTACGGCAGTAATCTGACATTGTAGCGGGTCAGTGCGGCATCGGAAAATAACATTTTCATCACGCTTTTGCTATTTTATCCCTTTGCTCTATTTGTTGCATTCCCAATATTCGTTTCTTCTCTGATGCCGGCAAACTGGCGTATTTATAACCATTTTTAGCTTTCACTGCTGCATTTTTTTGCGCTCGCCa                                                                                                                                                               >  2:104399/1‑250 (MQ=255)                                                                                    cAATCTGGCGTTGGATTTTTTCGATAGTGGTGCTCATTGTCTTGCTTCCTCAAACTTCTTTACGGCAGTAATCTGACATTGTAGCGGGTCAGTGCGGCATCGGAAAATAACATTTTCATCACGCTTTTGCTATTTTATCCCTTTGCTCTATTTGTTGCATTCCAAATATTCGTTTCTTCTCTGATGCCGGCAAACTGGCGTATTTATAACCATTTTTAGCTTTCACTGCTGCATTTTTTTGCGCTCGCCa                                                                                                                                                               >  2:82906/1‑250 (MQ=255)                                                                                                             aGTGGTGCTCATTGTCTTGCTTCCTCAAACTTCTTTACGGCAGTAATCTGACATTGTAGCGGGTCAGTGCGGCATCGGAAAATAACATTTTCATCACGCTTTTGCTATTTTATCCCTTTGCTCTATTTGTTGCATTCCAAATATTCGTTTCTTCTCTGATGCCGGCAAACTGGCGTATTTATAACCATTTTTAGCTTTCACTGCTGCATTTTTTTGCGCTCGCCAACGAAACGTATTTTTTAACAATaaa                                                                                                                                      <  1:67599/250‑1 (MQ=255)                                                                                                                      cATTGTCTTGCTTCCTCAAACTTCTTTACGGCAGTAATCTGACATTGTAGCGGGTCAGTGCGGCATCGGAAAATAACATTTTCATCACGCTTTTGCTATTTTATCCCTTTGCTCTATTTGTTGCATTCCAAATATTCGTTTCTTCTCTGATGCCGGCAAACTGGCGTATTTATAACCATTTTTAGCTTTCACTGCTGCATTTTTCTGCGCTCGCCAACGAAACGTATTTTTTAACAATAAAAGCTATTAAc                                                                                                                            <  2:44148/251‑1 (MQ=255)                                                                                                                                  tCCTCAAACTTCTTTACGGCAGTAATCTGACATTGTAGCGGGTCAGTGCGGCATCGGAAAATAACATTTTCATCACGCTTTTGCTATTTTATCCCTTTGCTCTATTTGTTGCATTCCAAATATTCATTTCTTCTCTGATGCCGGCAAACTGGCGTATTTATAACCATTTTTAGCTTTCACTGCTGCATTTTTTTGCGCTCGCCAACGAAACGTATTTTTTAACAATAAAAGCTATTAACTTTCtcttctt                                                                                                                 <  2:256774/250‑1 (MQ=255)                                                                                                                                                  cGGCAGTAATCTGACATTGTAGCGGGTCAGTGCGGCATCGGAAAATAACATTTTCATCACGCTTTTGCTATTTTATCCCTTTGCTCTATTTGTTGCATTCCAAATATTCGTTTCTTCTCTGATGCCGGCAAACTGGCGTATTTATAACCATTTTTAGCTTTCACTGCTGCATTTTTTTGCGCTCGCCAACGAAACGTATTTTTTAACAATAAAAGCTATTAACTTTCTCTTCTTCTATGCATTAGAatcat                                                                                                >  2:14596/1‑251 (MQ=255)                                                                                                                                                    gCAGTAATCTGACATTGTAGCGGGTCAGTGCGGCATCGGAAAATAACATTTTCATCACGCTTTTGCTATTTTATCCCTTTGCTCTATTTGTTGCATTCCAAATATTCGTTTCTTCTCTGATGCCGGCAAACTGGCGTATTTATAACCATTTTTAGCTTTCACTGCTGCATTTTTTTGCGCTCGCCAACGAAACGTATTTTTTAACAATAAAAGCTATTAACTTTCTCTTCTTCTATGCATTAGAAtcatca                                                                                              <  1:14596/251‑1 (MQ=255)                                                                                                                                                                 aTTGTAGCGGGTCAGTGCGGCATCGGAAAATAACATTTTCATCACGCTTTTGCTATTTTATCCCTTTGCTCTATTTGTTGCATTCCAAATATTCGTTTCTTCTCTGATGCCGGCAAACTGGCGTATTTATAACCATTTTTAGCTTTCACTGCTGCATTTTTTTGCGCTCGCCAACGAAACGTATTTTTTAACAATAAAAGCTATTAACTTTCTCTTCTTCTATGCATTAGAATCATCAAGTTTTGTAAATc                                                                                 <  1:292839/251‑1 (MQ=255)                                                                                                                                                                         gggTCAGTGCGGCATCGGAAAATAACATTTTCATCACGCTTTTGCTATTTTATCCCTTTGCTCTATTTGTTGCATTCCAAATATTCGTTTCTTCTCTGATGCCGGCAAACTGGCGTATTTATAACCATTTTTAGCTTTCACTGCTGCATTTTTTTGCGCTCGCCAACGAAACGTATTTTTTAACAATAAAAGCTATTAACTTTCTCTTCTTCTATGCATTAGAATCATCAAGTTTTGTAAATCAGACGc                                                                           >  2:282205/1‑249 (MQ=255)                                                                                                                                                                          ggTCAGTGCGGCATCGGAAAATAACATTTTCATCACGCTTTTGCTATTTTATCCCTTTGCTCTATTTGTTGCATTCCAAATATTCGTTTCTTCTCTGATGCCGGCAAACTGGCGTATTTATAACCATTTTTAGCTTTCACTGCTGCATTTTTTTGCGCTCGCCAACGAAACGTATTTTTTAACAATAAAAGCTATTAACTTTCTCTTCTTCTATGCATTAGAATCATCAAGTTTTGTAAATCAGACGCAgg                                                                        <  2:283870/251‑1 (MQ=255)                                                                                                                                                                             cAGTGCGGCATCGGAAAATAACATTTTCATCACGCTTTTGCTATTTTATCCCTTTGCTCTATTTGTTGCATTCCAAATATTCGTTTCTTCTCTGATGCCGGCAAACTGGCGTATTTATAACCATTTTTAGCTTTCACTGCTGCATTTTTTTGCGCTCGCCAACGAAACGTATTTTTTAACAATAAAAGCTATTAACTTTCTCTTCTTCTATGCATTAGAATCATCAAGTTTTGTAAATCAGACGCAGGCAt                                                                     <  1:282205/251‑1 (MQ=255)                                                                                                                                                                                        cGGAAAATAACATTTTCATCACGCTTTTGCTATTTTATCCCTTTGCTCTATTTGTTGCATTCCAAATATTCGTTTCTTCTCTGATGCCGGCAAACTGGCGTATTTATAACCATTTTTAGCTTTCACTGCTGCATTTTTTTGCGCTCGCCAACGAAACGTATTTTTTAACAATAAAAGCTATTAACTTTCTCTTCTTCTATGCATTAGAATCATCAAGTTTTGTAAATCAGACGCAGGCATGATAGACCTGc                                                          >  2:70821/1‑251 (MQ=255)                                                                                                                                                                                            aaaTAACATTTTCATCACGCTTTTGCTATTTTATCCCTTTGCTCTATTTGTTGCATTCCAAATATTCGTTTCTTCTCTGATGCCGGCAAACTGGCGTATTTATAACCATTTTTAGCTTTCACTGCTGCATTTTTTTGCGCTCGCCAACGAAACGTATTTTTTAACAATAAAAGCTATTAACTTTCTCTTCTTCTATGCATTAGAATCATCAAGTTTTGTAAATCAGACGCAGGCATGATAGACCTGCCttt                                                      <  1:76631/251‑1 (MQ=255)                                                                                                                                                                                             aaTAACATTTTCATCACGCTTTTGCTATTTTATCCCTTTGCTCTATTTGTTGCATTCCAAATATTCGTTTCTTCTCTGATGCCGGCAAACTGGCGTATTTATAACCATTTTTAGCTTTCACTGCTGCATTTTTTTGCGCTCGCCAACGAAACGTATTTTTTAACAATAAAAGCTATTAACTTTCTCTTCTTCTATGCATTAGAATCATCAAGTTTTGTAAATCAGACGCAGGCATGATAGACCTGCCTTTa                                                     <  2:305/251‑1 (MQ=255)                                                                                                                                                                                               tAACATTTTCATCACGATTTTGCTATTTTATCCCTTTGCTCTATTTGTTGCATTCCAAATATTCGTTTCTTCTCTGATGCCGGCAAACTGGCGTATTTATAACCATTTTTAGCTTTCACTGCTGCATTTTTTTGCGCTCGCCAACGAAACGTATTTTTTAACAATAAAAGCTATTAACTTTCTCTTCTTCTATGCATTAGAATCATCAAGTTTTGTAAATCAGACGCAGGCATGATAGACCTGCCTTTAc                                                    >  1:256320/1‑250 (MQ=255)                                                                                                                                                                                                      ttCATCACGCTTTTGCTCTTTTATCCCTTTGCTCTATTTGTTGCATTCCAAATATTCGTTTCTTCTCTGATGCCGGCAAACTGGCGTATTTATAACCATTTTTAGCTTTCACTGCTGCATTTTTTTGCGCTCGCCAACGAAACGTATTTTTTAACAATAAAAGCTATTACCTTTCTCTTCTTCTATGCATTCGCATCATCAAGTTTTGTAAATCAGACGCAGGCATGTTAGACCTGCCTTTACAGAGTGAc                                            >  2:362559/1‑251 (MQ=255)                                                                                                                                                                                                                                        tATTTGTTGCATTCCAAATATTCGTTTCTTCTCTGATGCCGGCAAACTGGCGTATTTATAACCATTTTTAGCTTTCACTGCTGCATTTTTTTGCGCTCGCCAACGAAACGTATTTTTTAACAATAAAAGCTATTAACTTTCTCTTCTTCTATGCATTAGAATCATCAAGTTTTGTAAATCAGACGCAGGCATGATAGACCTGCCTTTACAGAGGGACGCTCAGTGGCGCGGATAAACCGTATTTCGATCAc          >  2:312588/1‑251 (MQ=255)                                                                                                                                                                                                                                         aTTTGTTGCATTCCAAATATTCGTTTCTTCTCTGATGCCGGCAAACTGGCGTATTTATAACCATTTTTAGCTTTCACTGCTGCATTTTTTTGCGCTCGCCAACGAAACGTATTTTTTAACAATAAAAGCTATTAACTTTCTCTTCTTCTATGCATTAGAATCATCAAGTTTTGTAAATCAGACGCAGGCATGATAGACCTGCCTTTACAGAGGGACGCTCAGTGGCGCGGATAAACCGTATTTCGATCAc          <  1:143657/250‑1 (MQ=255)                                                                                                                                                                                                                                         aTTTGTTGCATTCCAAATATTCGTTTCTTCTATGATTCCGGCAAACTGGCGTCTTTATAACCATTTTTAGCTTTCAATGCTGTATTTTTTTGCGCTAGCCAACGAAACGTATTTTTTAACAATAAACGCTATTAACTTTCTCTTCTTCACTGCATTCGAATCATCAAGTTTTGTAAATCAGACGCAGGCATGATAGACCTGCCGTTACAGAGGGACGCTCAGTGGCGCGGATAAACCGTATTTCGATCACg         >  2:320052/1‑251 (MQ=255)                                                                                                                                                                                                                                             gttgCATTCCAAATATTCGTTTCTTCTCTGATGCCGGCAAACTGGCGTATTTATAACCATTTTTAGCTTTCACTGCTGCATTTTTTTGCGCTCGCCAACGAAACGTATTTTTTAACAATAAAAGCTATTAACTTTCTCTTCTTCTATGCATTAGAATCATCAAGTTTTGTAAATCAGACGCAGGCATGATAGACCTGCCTTTACAGAGGGACGCTCAGTGGCGCGGATAAACCGTATTTCGATCACGctct     <  2:107371/251‑1 (MQ=255)                                                                                                                                                                                                                                                gCATTCCAAATATTCGTTTCTTCTCTGATGCCGGCAAACTGGCGTATTTATAACCATTTTTAGCTTTCACTGCTGCATTTTTTTGCGCTCGCCAACGAAACGTATTTTTTAACAATAAAAGCTATTAACTTTCTCTTCTTCTATGCATTAGAATCATCAAGTTTTGTAAATCAGACGCAGGCATGATAGACCTGCCTTTACAGAGGGACGCTCAGTGGCGCGGATAAACCGTATTTCGATCACGCTCtgtg  >  1:233038/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      AAGCGCCTGGACTGCCTGGGCAGAGAAACCGCAGCTCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTTCGATAGTGGTGCTCATTGTCTTGCTTCCTCAAACTTCTTTACGGCAGTAATCTGACATTGTAGCGGGTCAGTGCGGCATCGGAAAATAACATTTTCATCACGCTTTTGCTATTTTATCCCTTTGCTCTATTTGTTGCATTTCAAATATTCGTTTCTTCTCTGATGCCGGCAAACTGGCGTATTTATAACCATTTTTAGCTTTCACTGCTGCATTTTTTTGCGCTCGCCAACGAAACGTATTTTTTAACAATAAAAGCTATTAACTTTCTCTTCTTCTATGCATTAGAATCATCAAGTTTTGTAAATCAGACGCAGGCATGATAGACCTGCCTTTACAGAGGGACGCTCAGTGGCGCGGATAAACCGTATTTCGATCACGCTCTGTG  >  NC_000913/1733982‑1734471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑