Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,652,331	T→C	intergenic (+2346/+397)	ydfD → / ← ynfP	lysis protein/protein YnfP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,652,331	0	T	C	100.0%	75.2 / NA	23	intergenic (+2346/+397)	ydfD/ynfP	lysis protein/protein YnfP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/11);  total (12/11)

TCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACTTCAGAGAGTATGGCAGAACAGAAAAAC‑AAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACC‑GTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGAT  >  NC_000913/1652097‑1652566                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                              tCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGGCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGc                                                                                                                                                                                                                                 >  1:258938/1‑249 (MQ=255)      ttATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGcagaaca                                                                                                                                                                                                                           <  2:159474/250‑1 (MQ=255)                    gCCCTTGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGCTCCGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCAGGGCATTCATCTTATTAAACCAGCAATCGACGCTCTCCGGAGGCAGCTTACATTTACGAGCATGAGCAAAGAGCATATCATTGATGTTCACCTCCGAGAGAATGGAAGAACAGAaaaaa‑aaaaaat                                                                                                                                                                                                            >  2:289819/1‑248 (MQ=255)                          gTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATAGGGTCATTCATCTTATTACGACAGCAAACGACGCTCTCCGGAGTCAGATGACATTAACGAAACTGACCAAAGAGCATATCATTGATGTTAACCTCAGAGATCATGGCAGAACATAAAAAC‑AAAAAAGCCCCttt                                                                                                                                                                                                     >  2:199254/1‑251 (MQ=255)                                 gtgtgCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATAAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAAC‑AAAAATGCACCTTTGTTTTTc                                                                                                                                                                                              <  2:258938/251‑1 (MQ=255)                                                         aGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAAC‑AAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTa                                                                                                                                                                       <  2:28577/250‑1 (MQ=255)                                                               gAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAAC‑AAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTaaaaa                                                                                                                                                                   <  2:196691/248‑1 (MQ=255)                                                                                tgatCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCACTCGGGGCCTTCCTCTTATTAAGCCAGCCATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAAAAGAAAAAA‑AAAAATGCAcc                                                                                                                                                                                                        >  2:185825/1‑194 (MQ=255)                                                                                tgatCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAAC‑AAAAATGCAcc                                                                                                                                                                                                        <  1:185825/194‑1 (MQ=255)                                                                                    cAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAAC‑AAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACC‑Gtt                                                                                                                                           <  2:196055/250‑1 (MQ=255)                                                                                        agaAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGGACCAAACGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCCGAGTCAGATGACATTTACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCCGAGAGTATGGcagaacagaa                                                                                                                                                                                                                        >  2:111090/1‑171 (MQ=255)                                                                                        agaAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGcagaacagaa                                                                                                                                                                                                                        <  1:111090/171‑1 (MQ=255)                                                                                                         ccGTTTCACGGTACCAAAAACACAGGCGGGCACCCATCTGGTCCTTTATCTTATTAAGCCAGCAATCGACGCTCTCCCGAGGCAGATGACATTTACGAGACTGAGCAAAGAGCCTATCATTGATGTTTACCTCAGAGAGTATGGCAGAACAGAAAAACCAAAAAT‑CACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGGAAAACATTATGGTGACCCTTTTACCTGTT‑ACTCAATAAGGCAGGTGTggg                                                                                                                     >  1:333591/1‑251 (MQ=255)                                                                                                               cACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAAACGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAAAGCATATCATTGATGTTCACCTCCGAGAGTATGGCAGAACAGAAAAAC‑AAAAATGCACCTTTTTTTTTTAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACC‑GTTGACTCAATAAAGCAGATGTGGGACGccg                                                                                                               >  2:129133/1‑249 (MQ=255)                                                                                                                                                                       cAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAAC‑AAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACC‑GTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTAt                                                       <  1:129133/251‑1 (MQ=255)                                                                                                                                                                              cGCTCTCCGGAGACAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAAC‑AAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACC‑GTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCctgct                                                >  1:132848/1‑251 (MQ=255)                                                                                                                                                                                 tatcCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATACCATTGATGTTCACCTCATAGAGTATGGCAGAACAGAAAAAC‑AACAATGCAACTTTGTATTTAAACCTGAAGCGTAAGCGAGAGTAAAAAATTATGGTGACCATTTTACC‑GTTGACAATATAAGGTAGATGTGGGATGCAGCGATATAACGTGCCGGACACCGCCATCGCATATACTATCAGTCGAGACATACTTAGGCCTGCTggg                                             >  2:205990/3‑250 (MQ=255)                                                                                                                                                                                  ctcCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAAC‑AAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACC‑GTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTc                                            >  1:305567/1‑251 (MQ=255)                                                                                                                                                                                           cAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAAC‑AAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACC‑GTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGc                                   <  2:40556/251‑1 (MQ=255)                                                                                                                                                                                                   aTTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAAC‑AAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACC‑GTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAAGCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTa                           <  2:366407/251‑1 (MQ=255)                                                                                                                                                                                                          agaCTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAAC‑AAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACC‑GTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGc                    >  1:55421/1‑251 (MQ=255)                                                                                                                                                                                                            aCTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAAC‑AAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACC‑GTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCAt                  >  2:125340/1‑251 (MQ=255)                                                                                                                                                                                                                            atCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAAC‑AAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACC‑GTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGAt  <  1:74035/251‑1 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                              TCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACTTCAGAGAGTATGGCAGAACAGAAAAAC‑AAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACC‑GTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGAT  >  NC_000913/1652097‑1652566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑