Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,623,555	T→C	Q99R (CAG→CGG)	dgcZ ←	diguanylate cyclase DgcZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,623,555	0	T	C	100.0%	55.1 / NA	19	Q99R (CAG→CGG)	dgcZ	diguanylate cyclase DgcZ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/10);  total (9/10)

GATTAAATGCCCGTAGGTATCATTAACCAATTTAAATCGGTCAATATCCAACAACATTAAATAAAGATTCAGAGGCTCAGCGTTGCGTAACTGATGATCAAAGGATTCATCAAGAACCCGACGACCCGGCAATCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGAAATGCGCGTCCTGCCAGTGATTTTCAACAATAGCCAGCATTAATTCCCGACCACAGTTATGCATATGTTGATGGGCAGAATCCATTAGCCGAACGTAAGGTAATTCATCGTTATCGAGTGGCCCCAGATGATCAATCCACCGACCAAACTGGCACAGTCCATAAGAATGGTTATCCGTTATTTCTGGCTTACTGGCATCTCTCGCGACCACGCTGTGAAACATACTCACCAGCCACTGGTAGTGGGCATCGATAG  >  NC_000913/1623311‑1623798                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    gATTAAATGCCCGTAGGTATCATTAACCAATTTAAATCGGTCAATATCCAACAACATTAAATAAAGATTCAGAGGCTCAGCGTTGCGTAACTGATGATCAAAGGATTCATCAAGAACCCGACGACCCGGCAATCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGAAATGCGCGTCCCGCCAGt                                                                                                                                                                                                                                               >  1:120491/1‑251 (MQ=255)                          aCCAATTTAAATCGGTCAATATCCAACAACATTAAATAAAGATTCAGAGGCTCAGCGTTGCGTAACTGATGATCAAAGGATTCATCAAGAACCCGACGACCCGGCAATCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGAAATGCGCGTCCCGCCAGTGATTTTCAACAATAGCCAGCATTa                                                                                                                                                                                                                       >  1:297796/1‑250 (MQ=255)                                   aaTCGGTCAATATCCAACAACATTAAATAAAGATTCAGAGGCTCAGCGTTGCGTAACTGATGATCAAAGGATTCATCAAGACCCCGACGACCCGGCAATCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGAAATGCGCTTCCCGCCAGTGATTTTCAACAATAGCCAGCATTAATTCCCGAcc                                                                                                                                                                                                             <  1:223983/251‑1 (MQ=255)                                           aaTACCCAACAACATAAAATAAAAATTCAGAGGCTCAGCGTTGCGTAACTGATGATAAAAGGTTTCATCAAGACCCCGACGCCCCGGCAATCCCGTCAAAACACCCATATTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTAAAAGGCGTCGAAATGCGCGTCCCGCCAGTGATTTTCAACAATAGCCAGCATTAATTCCCGACCACAGTTAt                                                                                                                                                                                                     <  2:361266/251‑1 (MQ=255)                                                           aaataaaGATTCAGAGGCTCAGCGTTGCGTAACTGATGATAAAAGGATTCATCAAGAACCCGACGACCCGGCAATCCCGACAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGAAATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAGGGCGTCGAAATGCGCGTCCCGCCAGTGATTTTCAACAATAGCCAGCATTAATTCCCGACCACAGTTATGCATATGTTGATGGGc                                                                                                                                                                                     <  1:239963/251‑1 (MQ=255)                                                             ataaaGATTCAGAGGCGCATCGTTGCGTAACTGATGATCAAAGGATCCATCAAGAACCCGACGACCCGGCAATCCCGTAAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGTATTCGGTTAATGCCGCAGTAAAAGAAACCAACCCCTCCTGAAAGGCTTCAAAATGCGCGTCCCGCCAGTGATTTTCAACAATAGCCAGCATTAATTCCCGACCACAGTTATGCATATGTTGATGGGCAg                                                                                                                                                                                   <  1:363203/251‑1 (MQ=255)                                                                                                     aaGGATTCATCAAGAACCCGACGACCCGGCAATCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTAATGCCGCAGAAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGAAATGCGCGTCCCGCCAGTGATTTTCAACAATAGCCAGCATTAATTCCCGACCACAGTTATGCATATGTTGATGGGCAGAATCCATTAGCCGAACGTAAGGTAATTCATCGTTATCGAg                                                                                                                                           <  2:48810/251‑1 (MQ=255)                                                                                                                       cgacgcCCCGGCAATCCCGTAAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGAAATGCGCGTCCCGCCAGTGATTTTCAACAATAGCCAGCATTAATTCCCGACCACAGTTATGCATATGTTGATGGGCAGAATCCATTAGCCGAACGTAAGGTAATTCATCGTTATCGAGTGGCCCCAGATGATCAAt                                                                                                                         <  1:246141/251‑1 (MQ=255)                                                                                                                                 acaaccccGACAAAACATCCATATTGCTACGGAGCGTCAGCAAATAAATTTTTTAATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTAGAAATGCGCGTCCCGCCAGTGATTTTCAACAATAGCCAGCATTAATTCCCGACCACAGTTATGCATATGTTGATGGGCAGAATCCATTAGCCGAACGTAAGGTAATTCAGCGTTATCGAGTGTCCCCAGATGATCAATCCACCGACCa                                                                                                               <  2:53146/246‑1 (MQ=255)                                                                                                                                  cAATCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGAAATGCGCGTCCCGCCAGTGATTTTCAACAATAGCCAGCATTAATTCCCGACCACAGTTATGCATATGTTGATGGGCAGAATCCATTAGCCGAACGTAAGGTAATTCATCGTTATCGAGTGGCCCCAGATGATCAATCCACCGACCa                                                                                                               >  2:267574/1‑250 (MQ=255)                                                                                                                                      cccGTCAAAACATCCATATTGCTACGGATCGTCAGCAAAAACATTTTCTCATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGAAATGCGCGTCCCGCCAGTGATTTTCAACAATAGCCAGCATTAATTCCCGACCACAGTTATGCATATGATGACGGGCAGACTCCATTAGCCGAAAGTAAGGTAATTCATCGTTATCGAGTGGCCCCAGATGCTAAATCTACCGACCAAACTg                                                                                                          >  1:178050/1‑251 (MQ=255)                                                                                                                                            aaaaCATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGAAATGCGCGTCCCGCCAGTGATTTTCAACAATAGCCAGCATTAATTCCCGACCACAGTTATGCATATGTTGATGGGCAGAATCCATTAGCCGAACGTAAGGTAATTCATCGTTATCGAGTGGCCCCAGATGATCAATCCACCGACCAAACTGGcac                                                                                                      >  2:9179/1‑249 (MQ=255)                                                                                                                                                             aCGGATCGTCAGCAAATAAATTTTGTAATCGGTTAATGCCGCAGTAAACGAAAGCAACCCCTCCTGAAAGGCGTCGGAATGCGCGTCCCGCCAGTGATTTTCAACAATAGCCAGCATTAATTCCCGACCACAGTTCTGCATATGTTGATGGGCAGAATCCATTAGCCGAACGTAAGGTAATTCATCGTTATCGAGTGGCCCCAGATGATCAATCCACCGACCACACTGGCACAGTCCATAAGAATGGTTAt                                                                                   >  2:189222/1‑251 (MQ=255)                                                                                                                                                                                         gcGGTTAATGCCTCAGAAAAAGAAAGCACCCCCTCCTAAAAGGCGTCGAAATGCGCGTCCCGCCAGTGATTTTCAACAATAGCCAGCATTAATTCCCGACCACAGTTATGCATATGTTGATGGGCAGAATCCATTAGCCGAACGTAAGGTAATTCATCGTTATCGAGTGGCCCCAGATGATCAATCCACCGACCAAACTGGCACAGTCCATAAGAATGGTTATCCGTTATTTCTGGCTTACTGGCAtctct                                                       <  1:335109/250‑1 (MQ=255)                                                                                                                                                                                            gTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGAAATGCGCGTCCCGCCAGTGATTTTCAACAATAGCCAGCATTAATTCCCGACCACAGTTATGCATATGTTGATGGGCAGAATCCATTAGCCGAACGTAAGGTAATTCATCGTTATCGAGTGGCCCCAGATGATCAATCCACCGACCAAACTGGCACAGTCCATAAGAATGGTTATCCGTTATTTCTGGCTTACTGGCATCTCTcgc                                                    >  2:324395/1‑251 (MQ=255)                                                                                                                                                                                                                          tcctGAAAGGCGTCGAAATGCGCGTCCCGCCAGTGATTTTCAACAATAGCCAGCATTAATTCCCGAACACAGTTATGCATATGTTGATGGGCAGAATCCATTAGCCGAACGTAAGGTAATTCATCGTTATCGAGTGGCCCCAGATGATCAATCCACCGACCAAACTGGCACAGTCCATAAGAATGGTTATCCGTTATTTCTGGCTTACTGGCATCTCTCGCGACCACGCTGTGAAACATACTCACCAGcc                       >  1:167277/1‑250 (MQ=255)                                                                                                                                                                                                                                       cGAAATGCGCGTCCCGCCAGTGATTTTCAACAATAGCCAGCATTAATTCCCGACCACAGTTATGCATATGTTGATGGGCAGAATCCATTAGCCGAACGTAAGGTAATTCATCGTTATCGAGTGGCCCCAGATGATCAATCCACCGACCAAACTGGCACAGTCCATAAGAATGGTTATCCGTTATTTCTGGCTTACTGGCATCTCTCGCGACCACGCTGTGAAACATACTCACCAGCCACTGGTAGTGGGc          <  1:267574/250‑1 (MQ=255)                                                                                                                                                                                                                                              cgcgTCCCGCCAGTGATTTTCAACAATAGCCAGCATTACTACCCGACCACAGAACTGCATACGTTGATGGGCAGAATCCATTCGCCGAACGTAACGTACTTCATCGTTATCGAGTGCCCCCAGATGATCAATCCACCGACCAAACTGGCACAGTCCATAAGAATGGTTATCCGTTATTTCTCGCTTACTGGCATCTCTCGCGACAACGCTGTGAAACATACTCACCAGCCACTGGTAGTGGGCACCGAt    >  1:173808/1‑249 (MQ=255)                                                                                                                                                                                                                                              cgcgTCCCGCCAGTGATTTTCAACAATAGCCAGCATTAATTCCCGACCACAGTTATGCATATGTTGATGGGCAGAATCCATTAGCCGAACGTAAGGTAATTCATCGTTATCGAGTGGCCCCAGATGATCAATCCACCGACCAAACTGGCACAGTCCATAAGAATGGTTATCCGTTATTTCTGGCTTACTGGCATCTCTCGCGACCACGCTGTGAAACATACTCACCAGCCACTGGTAGTGGGCATCGATAg  <  1:324395/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    GATTAAATGCCCGTAGGTATCATTAACCAATTTAAATCGGTCAATATCCAACAACATTAAATAAAGATTCAGAGGCTCAGCGTTGCGTAACTGATGATCAAAGGATTCATCAAGAACCCGACGACCCGGCAATCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGAAATGCGCGTCCTGCCAGTGATTTTCAACAATAGCCAGCATTAATTCCCGACCACAGTTATGCATATGTTGATGGGCAGAATCCATTAGCCGAACGTAAGGTAATTCATCGTTATCGAGTGGCCCCAGATGATCAATCCACCGACCAAACTGGCACAGTCCATAAGAATGGTTATCCGTTATTTCTGGCTTACTGGCATCTCTCGCGACCACGCTGTGAAACATACTCACCAGCCACTGGTAGTGGGCATCGATAG  >  NC_000913/1623311‑1623798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑