Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,303,286	A→G	T130A (ACC→GCC)	oppB →	murein tripeptide ABC transporter/oligopeptide ABC transporter inner membrane subunit OppB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,303,286	0	A	G	100.0%	88.4 / NA	28	T130A (ACC→GCC)	oppB	murein tripeptide ABC transporter/oligopeptide ABC transporter inner membrane subunit OppB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/13);  total (15/13)

GAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGTGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTAAATTCATGATATTGCCGATGGTGGCGTTGTCACTCGCTTATATCGCCAGTATTGCGCGTATTACCCGTGGCTCTATGATTGAAGTATTACACTCCAATTT  >  NC_000913/1303046‑1303512                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                   gAAGCGAAATATCATCTTAATGATCCGATCATGCCACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGTTATTGCCGCTTTAAAACAAAACACCAAATGGGACTATGCCGTGATggg                                                                                                                                                                                                                          >  1:371656/1‑251 (MQ=255) gAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATGCCGTGATggg                                                                                                                                                                                                                          >  1:371651/1‑251 (MQ=255)  aaGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATGCCGTGATggtg                                                                                                                                                                                                                         <  1:134671/251‑3 (MQ=255)             catcTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATGCCGTGATGGGGCTGGCAATGa                                                                                                                                                                                                               <  1:66897/250‑1 (MQ=255)              atcgtAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGACGCAGCATTTTTCCTTGCGGTAATACTGGGTGGTAGTGCTGGCGTTAGTGCCGCATTAAAACAAAACTCCAAATGGGACTATGACGTGATGGGGCTTGCGATGAca                                                                                                                                                                                                             <  2:52307/247‑2 (MQ=255)                cTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATGCCGTGATGGGGCTGGCAATGACCgg                                                                                                                                                                                                           >  1:357592/1‑251 (MQ=255)                                              aGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATGCCGTGATGGGGCTGGCAATGACCGGGGTTGTTAtcccataagcgctaacttaagg                                                                                                                                                                             >  2:299689‑M1/1‑229 (MQ=255)                                                                     cGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATGCCGTGATGGGGCTGGCAATGACCGGGGTTGTTAtcccataagcgctaacttaagggttgtggtattacgcctgatatg                                                                                                                                                      <  2:158042‑M1/251‑46 (MQ=255)                                                                                                     attattCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATGCCGTGATGGGGCTGGCAATGACCGGGGTTGTTAtcccataagcgctaacttaagggttgtggtattacgcctgatatgatttaacgtgccgatgaattactctcacgat                                                                                                                       >  2:192976‑M1/1‑174 (MQ=255)                                                                                                                                               atgCCAAACTGGGAACCGAAGCATTTTTCATTGCGGGAATACTAGGTGTTAGTGCTGTCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACAATGCCGTGATGGGGCTGGCAGTGACCGGGGGTGTTAtcccataagcactaacttaagggtttgggtatgacgcctgatatgatttaacgtgccgatgaattactctcacgataactggtcagcaattctggcccgtattggtaagccagaagaac                                                                            >  2:197077‑M1/2‑132 (MQ=255)                                                                                                                                                   cAAACTGGGAGACGCAGCCTTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATGCCGTGATGGGGCTGGCAATGACCGGGGTTGTTAtcccataagcgctaacttaagggttgtggtcttacgcctgatatgatttaacgtgccgatgaattactctcacgataactggtcagcaattctggcccatattggtaagcccgaagaactgg                                                                         >  2:122548‑M1/1‑128 (MQ=255)                                                                                                                                                     aaCTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATGCCGTGATGGGGCTGGCAATGACCGGGGTTGTTAtcccataagcgctaacttaagggttgtggtattacgcctgatatgatttaacgtgccgatgaattactctcacgataactggtcagcaattctggcccatattggtaagcccgaagaactggat                                                                       >  2:201433‑M1/1‑126 (MQ=255)                                                                                                                                                              ccGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATGCCGTGATGGGGCTGGCAATGACCGGGGTTGTTAtcccataagcgctaacttaagggttgtggtattacgcctgatatgatttaacgtgccgatgaattactctcacgataactggtcatcaattcttgcccatattggtaatcccgaagaactggatacttcggcac                                                             <  2:371656‑M1/251‑135 (MQ=255)                                                                                                                                                              ccGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATGCCGTGATGGGGCTGGCAATGACCGGGGTTGTTAtcccataagcgctaacttaagggttgtggtattacgcctgatatgatttaacgtgccgatgaattactctcacgataactggtcagcaattctggcccatattggtaagcccgaagaactggatacttcggcac                                                             <  2:371651‑M1/251‑135 (MQ=255)                                                                                                                                                                      tttttCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATGCCGTGATGGGGCTGGCAATGACCGGGGTTGTTATcc                                                                                                                                                                                                <  2:112747/112‑1 (MQ=255)                                                                                                                                                                      tttttCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATGCCGTGATGGGGCTGGCAATGACCGGGGTTGTTATcc                                                                                                                                                                                                >  1:112747/1‑112 (MQ=255)                                                                                                                                                                         ttCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATGCCGTGATGGGGCTGGCAATGACCGGGGTTGTTAtcccataagcgctaacttaagggttgtggtattacgcctgatatgatttaacgtgccgatgaattactctcacgataactggtcagcaattctggcccatattggtaagcccgaagaactggatacttcggcacgtaatgccggg                                                  >  1:84680‑M1/1‑106 (MQ=255)                                                                                                                                                                          tCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATGCCGTGATGGGGCTGGCAATGACCGGGGTTGTTAtcccataagcgctaacttaagggttgtggtattacgcctgatatgatttaacgtgccgatgaattactctcacgataactggtcagcaattctggcccatattgg                                                                                          <  1:83923‑M1/210‑106 (MQ=255)                                                                                                                                                                          tCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATGCCGTGATGGGGCTGGCAATGACCGGGGTTGTTAtcccataagcgctaacttaagggttgtggtattacgcctgatatgatttaacgtgccgatgaattactctcacgataactggtcagcaattctggcccatattgg                                                                                          >  2:83923‑M1/1‑105 (MQ=255)                                                                                                                                                                                       aCTGGGTGTTAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTAGGCCGTGATGGGGCTGGCAATGACCGGGGTTGGTAtcccataagcgctaacttaagggttgtggtattacgcctgataggatttaacgtgccgatgaattactctcacgataactggtcagcaattctggcccatatgggtaagcccgaagaactggatacttcggcacgtaatgccggggcgctaacccgcc                                     <  2:96010‑M1/250‑159 (MQ=255)                                                                                                                                                                                         tggttgttagtGCTGGAGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATGTCGTGATGGGGCTGGCAATGACCGGGGTTGTTAtcccataagcgctaccttaagggttgtgggattacgcctgatctgatttaacgtgccgatgaattactctcacgataactggacagcaattctggcccatattggtaagcccgaagaactggatacttcggcacgtaatgccggggctctaacccgccgcc                                  >  2:196767‑M1/5‑90 (MQ=255)                                                                                                                                                                                                 aGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATGCCGTGATGGGGCTGGCAATGACCGGGGTTGTTAtcccataagcgctaacttaagggttgtggtattacgcctgatatgatttaacgtgccgatgaattactctcacgataactggtcagcaattctggcccatattggtaagcccgaagaactggatacttcggcacgtaatgccggggctctaacccgccgccgcgaaatt                          >  1:120259‑M1/1‑82 (MQ=255)                                                                                                                                                                                                 aGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATGCCGTGATGGGGCTGGCAATGACCGGGGTTGTTAtcccataagcgctaacttaagggttgtggtattacgcctgatatgatttaacgtgccgatgaattactctcacgataactggtcagcaattctggcccatattggtaagcccgaagaactggatacttcggcacgtaatgccggggctctaacccgccgccgcgaaat                           <  1:350353‑M1/250‑169 (MQ=255)                                                                                                                                                                                                  gTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATGCCGTGATGGGGCTGGCAATGACCGGGGTTGTTAtcccataagcgctaacttaagggttgtggtattacgcctgatatgatttaacgtgccgatgaattactctcacgataactggtcagcaattctggcccatattggtaagcccgaagaactggatacttcggcacgtaatgccggggctctaacccgccgccgcgaaattc                         <  1:227851‑M1/251‑171 (MQ=255)                                                                                                                                                                                                       ggCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATGCCGTGATGGGGCTGGCAATGACCGGGGTTGTTAtcccataagcgctaacttaagggttgtggtattacgcctgatatgatttaacgtgccgatgaattactctcacgataactggtcagcaattctggcccatattggtaagcccgaagaactggatacttcggcacgtaatgccggggctctaacccgccgccgcgaaattcgtga                     <  1:201433‑M1/250‑175 (MQ=255)                                                                                                                                                                                                          gTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATGCCGTGATGGGGCTGGCAATGACCGGGGTTGTTAtcccataagcgctaacttaagggttgtggtattacgcctgatatgatttaacgtgccgatgaattactctcacgataactggtcagcaattctggcccatattggtaagcccgaagaactggatacttcggcacgtaatgccggggctctaacccgccgccgcgaaattcgtgatgct                 <  2:120259‑M1/251‑179 (MQ=255)                                                                                                                                                                                                          gTTATTGCCGCATTAAAACAAAACACCAAATGAGACTATGCCGTGATGCAGCTCGCAATGACCGGGGTTGTTAtcccatacgcgctaccttaagggttgtggtattacgcctgatatgatttaacgtgccgatgaattactatcacgataactggtcagcaattctggcccatattggtaagcccgaagcactggatacttcggcacgtaatgccggggctctaacccgccgccgcgaaattcgtgatgct                 >  1:22369‑M1/1‑73 (MQ=255)                                                                                                                                                                                                                         aaacaaaacaCCAAATGGGACTATGCCGTGATGGGGCTGGCAATGACCGGGGTTGTTAtcccataagcgctaacttaagggttgtggtattacgcctgatatgatttaacgtgccgatgaattactctcacgataactggtcagcaattctggcccatattggtaagcccgaagaactggatacttcggcacgtaatgccggggctctaacccgccgccgcgaaattcgtgatgctgcaactctgctacgt  >  1:119437‑M1/1‑58 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                   GAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGTGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTAAATTCATGATATTGCCGATGGTGGCGTTGTCACTCGCTTATATCGCCAGTATTGCGCGTATTACCCGTGGCTCTATGATTGAAGTATTACACTCCAATTT  >  NC_000913/1303046‑1303512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑