Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,272,482 A→G intergenic (+133/‑25) chaB → / → chaC putative cation transport regulator ChaB/glutathione‑specific gamma‑glutamylcyclotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,272,4820AG100.0% 90.2 / NA 29intergenic (+133/‑25)chaB/chaCputative cation transport regulator ChaB/glutathione‑specific gamma‑glutamylcyclotransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/12);  total (17/12)

AAGATCGGCGTGATGACGCCAGTCGCGAAGAAACCGCGCATAAAGTGGCCTGGGCTGCTGTGAAGCATGAATATGCCAAAGGGGATGATGATAAATGGCATAAAAAATCGTAAAACCGGTCGCTT‑AGTTAAAGCTATTCGTGCGGTGTTGCCTTGC‑AAGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCC‑GGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGT  >  NC_000913/1272237‑1272721
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                               
aagaTCGGCGTGATGACGCCAGTCGCGAAGAAACCGCGCATAAAGTGGCCTGGGCTGCTGTGAAGCATGAATATGCCAAAGGGGATGATGATAAATGGCATAAAAAATCGTAAAACCGGTCGCTT‑AGTTAAAGCTATTCGTGCGGTGTTGCCTTGC‑AAGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCC‑GGAAGACACCAAAAAGTTGTCGCAGGGGAGTAt                                                                                                                                                                                                                                            <  1:28941/251‑1 (MQ=255)
                              aaaCCGCGCATAACGTGGCCTGGGCTGCTGTGAAGCATGAATATGCCAAAGGGGATGATGATAAATGGCATAAAAAATCGTAAAACCGGTCGCTT‑AGTTAAAGCTATTCGTGCGGTGTTGCCTTGC‑AAGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGGAGTAAAAATGACC‑GGAAGACACCAAAAAGTTGTCGCAGGGGAGTATGCAGGGGCGGAAGTGAAAGGGGATAAcgcg                                                                                                                                                                                                              >  2:69436/1‑251 (MQ=255)
                                         aaaGTGGCCTGGGCTGCTGTGAAGCATGAATATGCCAAAGGGGATGATGATAAATGGCATAAAAAATCGTAAAACCGGTCGCTT‑AGTTAAAGCTATTCGTGCGGTGTTGCCTTGC‑AAGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCC‑GGAAGACACCAAAAAGTTGTCGCAGGGGAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGGGATTTCTtg                                                                                                                                                                                                    >  2:162403/1‑250 (MQ=255)
                                                                     aaTATGCCAAAGGGGATGATGATAAATGGCATAAAAAATCGTAAAACCGGTCGCTT‑AGTTAAAGCTATTCGTGCGGTGTTGCCTTGC‑AAGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCC‑GGAAGACACCAAAAAGTTGTCGCAGGGGAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTgg                                                                                                                                                                       <  1:174880/251‑1 (MQ=255)
                                                                      atatGCCAAAGGGGATGATGATAAATGGCATAAAAAATCGTAAAACCGGTCGCTT‑AGTTAAAGCTATTCGTGCGGTGTTGCCTTGC‑AAGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCC‑GGAAGACACCAAAAAGTTGTCGCAGGGGAGTATGCAGTGGCGGAAGGGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGt                                                                                                                                                                      >  2:41913/1‑251 (MQ=255)
                                                                          gCCAAAGGGGATGATGATAAATGGCATAAAAAATCGTAAAACCGGTCGCTT‑AGTTAAAGCTATTCGTGCGGTGTTGCCTTGC‑AAGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCC‑GGAAGACACCAAAAAGTTGTCGCAGGGGAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGcc                                                                                                                                                                   >  2:361652/1‑250 (MQ=255)
                                                                             aaaGGGGATTATGATAAAATGCCTAAAAAATCGTAAAAACCGTCGCTTTATTTAAA‑CTTTTCGTGCGGGGGTGCCTTGCCAGGGGGTCGGTG‑AATGCAAATTGTCCCCGTAATGGTTTCAAAATGGGGAGTAAAAAAGTCCCGGAAGAC‑CCAAAAAGTTGTCGCAGGGGAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTg                                                                                                                                                               <  1:61195/251‑1 (MQ=255)
                                                                                                ggCATAAAAAATCGTAAAACCGGTCGCTT‑AGTTAAAGCTATTCGTGCGGTGTTGCCTTGC‑AAGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCC‑GGTAGACACCAAAAAGTTGTCGCAGGGGAGTATGCAGTGGCGGAAGTGTAAGGGGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTc                                                                                                                                            >  2:258632/1‑251 (MQ=255)
                                                                                                     aaaaaaTCGTAAAACCGGTCGCTT‑AGTTAAAGCTATTCGTGCGGTGTTGCCTTGC‑AAGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCC‑GGAAGACACCAAAAAGTTGTCGCAGGGGAGTATGCAGTGGCGGAAGCGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTcagcag                                                                                                                                       <  2:109885/251‑1 (MQ=255)
                                                                                                         aaTCGTAAAACCGGTCGCTT‑AGTTAAAGCTATTCGTGCGGTGTTGCCTTGC‑AAGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCC‑GGAAGACACCAAAAAGTTGTCGCAGGGGAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGaaca                                                                                                                                   <  2:325599/251‑1 (MQ=255)
                                                                                                             gTAAAACCGGTCGCTT‑AGTTAAAGCTATTCGTGCGGTGTTGCCTTGC‑AAGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCC‑GGAAGACACCAAAAAGTTGTCGCAGGGGAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACgg                                                                                                                               <  1:361652/251‑1 (MQ=255)
                                                                                                                      gTCGCTT‑AGTTAAAGCTCTTCGTGCGGAGTTGACTTGC‑AAGTGGTACGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCC‑GGAAGACACCAAAAAGTTGTCGCAGGGGAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCg                                                                                                                      >  1:127431/1‑251 (MQ=255)
                                                                                                                        cGCTT‑AGTTAAAGCTATTCGTGCGGTGTTGCCTTGC‑AAGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAATATGTCC‑GGAAGACACCAAAAAGTTGTCGCAGGGGAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCt                                                                                                                    <  1:359635/251‑1 (MQ=255)
                                                                                                                          cTT‑AGTTAAAGCTATTCGTGCGGTGTTGCCTTGC‑AAGTGGTCCGTGGATTGCATATTGTCTCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCC‑GGAAGACACCAAAAAGTTGTCGCAGGGGAGTATGCAGTGGc                                                                                                                                                                                                                                    <  2:188153/137‑1 (MQ=255)
                                                                                                                          cTT‑AGTTAAAGCTATTCGTGCGGTGTTGCCTTGC‑AAGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCC‑GGAAGACACCAAAAAGTTGTCGCAGGGGAGTATGCAGTGGc                                                                                                                                                                                                                                    >  1:188153/1‑137 (MQ=255)
                                                                                                                                                ggTGTTGCCTTGC‑AAGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCC‑GGAAGACACCAAAAAGTTGTCGCAGGGGAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGAc                                                                                             >  1:156135/1‑251 (MQ=255)
                                                                                                                                                          tGC‑AAGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCC‑GGAAGACACCAAAAAGTTGTCGCAGGGGAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAggg                                                                                   >  1:201237/1‑251 (MQ=255)
                                                                                                                                                                gTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCC‑GGAAGACACCAAAAAGTTGTCGCAGGGGAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCgg                                                                              <  1:294947/251‑1 (MQ=255)
                                                                                                                                                                  ggTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCC‑GGAAGACACCAAAAAGTTGTCGCAGGGGAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCTGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGGCGACCTGATGAGGGACCGgtg                                                                            >  2:86223/1‑251 (MQ=255)
                                                                                                                                                                  ggTCCGTGGATTGCATATTGTACCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCC‑GGAAGACACCAAAAAGTTGTCGCAGGGGAGTATGCAGTGGCGGAAGTGTAAGGTGATCACGCGTGATTTCTTGCTGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGgtg                                                                            >  1:29643/1‑251 (MQ=255)
                                                                                                                                                                           aTTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCC‑GGAAGACACCAAAAAGTTGTCGCAGGGGAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGGCCGGTGTGGATCTTc                                                                   >  1:76182/1‑251 (MQ=255)
                                                                                                                                                                                    tGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCC‑GGAAGACACCAAAAAGTTGTCGCAGGGGAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATggg                                                          >  1:186015/1‑250 (MQ=255)
                                                                                                                                                                                         cGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCC‑GGAAGACACCAAAAAGTTGTCGCAGGGGAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACgg                                                                                                                               >  1:71765/1‑177 (MQ=255)
                                                                                                                                                                                         cGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCC‑GGAAGACACCAAAAAGTTGTCGCAGGGGAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACgg                                                                                                                               <  2:71765/177‑1 (MQ=255)
                                                                                                                                                                                                     cAAAATGAGCAGTAAAAATGTCC‑GGAAGACACCAAAAAGTTGTCGCAGGGGAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATcc                                         >  1:200317/1‑251 (MQ=255)
                                                                                                                                                                                                       aaaTGAGCAGTAAAAATGTCC‑GGAAGACACCAAAAAGTTGTCGCAGGGGAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGGGGATCTTCGGCTATGGATCGTTGAtg                                                 <  1:297087/241‑1 (MQ=255)
                                                                                                                                                                                                       aaaTGAACAGTAAAAATGTCC‑GGAAGACACCAAAAAGTTGTCGCAGGGGAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGCTTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGGTCTTCGGCTAGGGATCGTTGAtg                                                 >  2:297087/1‑241 (MQ=255)
                                                                                                                                                                                                            aGCAGTAAAAATGTCC‑GGAAGACACCAAAAAGTTGTCGCAGGGGAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTg                                  <  1:366598/251‑1 (MQ=255)
                                                                                                                                                                                                                                             ttGTCGCAGGGGAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCAATTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGGGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCCCTGGGGTTTACCGAATCGTGCACCGGAACACTggg  >  2:263696/1‑250 (MQ=255)
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                               
AAGATCGGCGTGATGACGCCAGTCGCGAAGAAACCGCGCATAAAGTGGCCTGGGCTGCTGTGAAGCATGAATATGCCAAAGGGGATGATGATAAATGGCATAAAAAATCGTAAAACCGGTCGCTT‑AGTTAAAGCTATTCGTGCGGTGTTGCCTTGC‑AAGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCC‑GGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGT  >  NC_000913/1272237‑1272721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑