Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,228,559	A→G	T161A (ACG→GCG)	ycgM →	putative isomerase/hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,228,559	0	A	G	100.0%	107.8 / NA	34	T161A (ACG→GCG)	ycgM	putative isomerase/hydrolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/23);  total (11/23)

GCGTCAGGCTACGGAAGAGCATGTCCGCAAAGCCATTGCCGGTTATGGCGTGGCGCTCGATCTGACATTGCGTGATGTTCAGGGAAAAATGAAGAAAGCCGGGCAGCCGTGGGAAAAGGCTAAAGCGTTTGATAACTCTTGTCCGCTTTCCGGGTTTATTCCCGCGGCGGAATTTACCGGCGACCCGCAAAATACAACGCTGAGCCTGAGCGTAAACGGCGAACAACGCCAGCAAGGTACGACTGCGGACATGATCCATAAAATCGTTCCGCTGATCGCTTATATGAGCAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGT  >  NC_000913/1228321‑1228805                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                       gCGTCAGGCTACGGAAGAGCATGTCCGCAAAGCCATTGCCGGTTATGGCGTGGCGCTCGATCTGACATTGCGTGATGTTCAGGGAAAAATGAAGAAAGCCGGGCAGCCGTGGGAAAAGGCTAAAGCGTTTGATAACTCTTGTCCGCTTTCCGGGTTTATTCCCGCGGCGGAATTTACCGGCGACCCGCAAAATACAACGCTGAGCCTGAGCGTAAACGGCGAACAACGCCAGCAAGGTGCGACTGCGGa                                                                                                                                                                                                                                              <  1:99353/249‑1 (MQ=255)           aCGGAAGAGCATGTCCGCAAAGCCATTGCCGGTTATGGCGTGTCGCGCGATCTGACATTGCGTGATGTTCAGGGAAAAATGAAGAAAGCCGGGCAGCCGTGGGAAAAGGCTAAAGCGTTTGATAACTCTTTTCCGCTTTCCGGGTTTATTCCCGCGGCGGAATTTACCGGCGACCCGCAAAATACAACGCTGAGCCTGAGCGTAAACTGCGAACAACTCCAGCAAGGGGCGACTGCGGACATGATCCAt                                                                                                                                                                                                                                    <  2:159968/249‑1 (MQ=255)                    cATGTCCGCAAAGCCATTGCCGGTTATGGCGTGGCGCTCGATCTGACATTGCGTGATGTTCAGGGAAAAATGAAGAAAGCCGGGCAGCCGTGGGAAAAGGCTAAAGCGTTTGATAACTCTTGTCCGCTTTCCGGGTTTATTCCCGCGGCGGAATTTACCGGCGACCCGCAAAATACAACGCTGAGCCTGAGCGTAAACGGCGAACAACGCCAGCAAGGTGCGACTGCGGACATGATCCATAAAATCGTTcc                                                                                                                                                                                                                         >  2:272258/1‑251 (MQ=255)                       gTCCGCAAAGCCATTGCCGGTTATGGCGTGGCGCTCGATCTGACATTGCGTGATGTTCAGGGAAAAATGAAGAAAGCCGGGCAGCCGTGGGAAAAGGCTAAAGCGTTTGATAACTCTTGTCCGCTTTCCGGGTTTATTCCCGCGGCGGAATTTACCGGCGACCCGCAAAATACAACGCTGAGCCTGAGCGTAAACGGCGAACAACGCCAGCAAGGTGCGACTGCGGACATGATCCATAAAATCGTTCCGc                                                                                                                                                                                                                       <  2:130355/250‑1 (MQ=255)                                      gCCGGTTATGGCGTGGCGCTCGATCTGACATTGCGTGATGTTCAGGGAAAAATGAAGAAAGCCGGGCAGCCGTGGGAAAAGGCTAAAGCGTTTGATAACTCTTGTCCGCTTTCCGGGTTTATTCCCGCGGCGGAATTTACCGGCGACCCGCAAAATACAACGCTGAGCCTGAGCGTAAACGGCGAACAACGCCAGCAAGGTGCGACTGCGGACATGATCCATAAAATCGTTCCGCTGATCGCTTATATGa                                                                                                                                                                                                        <  1:26059/250‑1 (MQ=255)                                               ggcgtggcgCTCGATCTGACATTGCGTGATGTTCAGGGAAAAATGAAGAAAGCCGGGCAGCCGTGGGAAAAGGCTAAAGCGTTTGATAACTCTTGTCCGCTTTCCGGGTTTATTCCCGCGGCGGAATTTACCGGCGACCCGCAAAATACAACGCTGAGCCTGAGCGTAAACGGCGAACAACGCCAGCAAGGTGCGACTGCGGACATGATCCATAAAATCGTTCCGCTGATCGCTTATATGAGCAAGttttt                                                                                                                                                                                              <  2:39694/251‑1 (MQ=255)                                                                gaaaTTGCGTGATGTTCAGGGAAAAATGAAGAAAGCCGGGCAGCCGTGGGAAAAGGCTAAAGCGTTTGATAACTCTTGTCCGCTTTCCGGGTTTATTCCCGCGGCGGAATTTACCGGCGACCCGCAAAATACAACGCTGAGCCTGAGCGTAAACGGCGAACAACGCCAGCAAGGTGCGACTGCGGACATGATCCATAAAATCGTTCCGCTGATCGCTTATATGAGCAAGTTTTTTACCCTCAAGGcc                                                                                                                                                                                 <  1:46445/244‑1 (MQ=255)                                                                   aTTGCGTGATGTTCAGGGAAAAATGAAGAAAGCCGGGCAGCCGTGGGAAAAGGCTAAAGCGTTTGATAACTCTTGTCCGCTTTCCGGGTTTATTCCCGCGGCGGAATTTACCGGCGACCCGCAAAATACAACGCTGAGCCTGAGCGTAAACGGCGAACAACGCCAGCAAGGTGCGACTGCGGACATGATCCATAAAATCGTTCCGCTGATCGCTTATATGAGCAAGTTTTTTCCCCTCAAGGCCGGTGACg                                                                                                                                                                          <  2:91727/251‑1 (MQ=255)                                                                       cGTGATGTTCAGGGAAAAATGAAGAAAGCCGGGCAGCCGTGGGAAAAGGCTAAAGCGTTTGATAACTCTTGTCCGCTTTCCGGGTTTATTCCCGCGGCGGAATTTACCGGCGACCCGCAAAATACAACGCTGAGCCTGAGCGTAAACGGCGAACAACGCCAGCAAGGTGCGACTGCGGACATGATCCATAAAATCGTTCCGCTGATCGCTTATATGAGCAAGTTTTTTACCCTCAAGGCCGGTGACGTtgg                                                                                                                                                                      >  2:364502/1‑250 (MQ=255)                                                                                   ggAAAAATGAAGAAAGCCGGGCAGCCGTGGGAAAAGGCTAAAGCGTTTGATAACTCTTGTCCGCTTTCCGGGTTTATTCCCGCGGCGGAATTTACCGGCGACCCGCAAAATACAACGCTGAGCCTGAGCGTAAACGGCGAACAACGCCAGCAAGGTGCGACTGCGGACATGATCCATAAAATCGTTCCGCTGATCGCTTATATGAGCAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCAc                                                                                                                                                          <  1:21558/251‑1 (MQ=255)                                                                                                          gCCGTGGGAAAAGGCTAAAGCGTTTGATAACTCTTGTCCGCTTTCCGGGTTTATTCCCGCGGCGGAATTTACCGGCGACCCGCAAAATACAACGCTGAGCCTGAGCGTAAACGGCGAACAACGCCAGCAAGGTGCGACTGCGGACATGATCCATAAAATCGTTCCGCTGATCGCTTATATGAGCAAGTTTTTTACCCTCAAGGCCg                                                                                                                                                                                >  1:293132/1‑206 (MQ=255)                                                                                                          gCCGTGGGAAAAGGCTAAAGCGTTTGATAACTCTTGTCCGCTTTCCGGGTTTATTCCCGCGGCGGAATTTACCGGCGACCCGCAAAATACAACGCTGAGCCTGAGCGTAAACGGCGAACAACGCCAGCAAGGTGCGACTGCGGACATGATCCATAAAATCGTTCCGCTGATCGCTTATATGAGCAAGTTTTTTACCCTCAAGGCCg                                                                                                                                                                                <  2:293132/206‑1 (MQ=255)                                                                                                                 gAAAAGGCTAAAGCGTTTGATAACTCTTGTCCGCTTTCCGGGTTTATTCCCGCGGCGGAATTTACCGGCGACCCGCAAAATACAACGCTGAGCCTGAGCGTAAACGGCGAACAACGCCAGCAAGGTGCGACTGCGGACATGATCCATAAAATCGTTCCGCTGATCGCTTATATGAGCAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCgg                                                                                                                            >  1:191096/1‑251 (MQ=255)                                                                                                                  aaaaGGCTAAAGCGTTTGATAACTCTTGTCCGCTTTCCGGGTTTATTCCCGCGGCGGAATTTACCGGCGACCCGCAAAATACAACGCTGAGCCTGAGCGTAAACGGCGAACAACGCCAGCAAGGTGCGACTGCGGACATGATCCATAAAATCGTTCCGCTGATCGCTTATATGAGCAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGt                                                                                                                           <  2:89611/251‑1 (MQ=255)                                                                                                                     agtcTAAAGCGTTTGATAACTATTGTCCGCTTTCCGGGTTTATTCCCGCGGCGGAATTTACCGGCGACCCGCAAAATACAACGCTGAGCCTGAGCGTAAACGGCGAACAACGCCAGCAAGGTGCGACTGCGGACATGATCCATAAAATCGTTCCGCTGATCGCTTATATGAGCAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAACGCGGtgat                                                                                                                        >  2:54086/4‑251 (MQ=255)                                                                                                                     aGGCTAAAGCGTTTGATAACTCTTGTCCGCTTTCCGGGTTTATTCCCGCGGCGGAATTTACCGGCGACCCGCAAAATACAACGCTGAGCCTGAGCGTAAACGGCGAACAACGCCAGCAAGGTGCGACTGCGGACATGATCCATAAAATCGTTCCGCTGATCGCTTATATGAGCAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGtgat                                                                                                                        <  2:304409/251‑1 (MQ=255)                                                                                                                       gCTAAAGCGTTTGATAACTCTTGTCCGCTTTCCGGGTTTATTCCCGCGGCGGAATTTACCGGCGACCCGCAAAATACAACGCTGAGCCTGAGCGTAAACGGCGAACAACGCCAGCAAGGTGCGACTGCGGACATGATCCATAAAATCGTTCCGCTGATCGCTTATATGAGCAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGtgatg                                                                                                                       >  2:76571/1‑250 (MQ=255)                                                                                                                                 ttGATAACTCTTGTCCGCTTTCCGGGTTTATTCCCGCGGCGGAATTTACCGGCGACCCGCAAAATACAACGCTGAGCCTGAGCGTAAACGGCGAACAACGCCAGCAAGGTGCGACTGCGGACATGATCCATAAAATCGTTCCGCTGATCGCTTATATGAGCAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGt                                                                                                             <  1:160511/250‑1 (MQ=255)                                                                                                                                             gTCCGCTTTCCGGGTTTATTCCCGCGGCGGAATTTACCGGCGACCCGCAAAATACAACGCTGAGCCTGAGCGTAAACGGCGAACAACGCCAGCAAGGTGCGACTGCGGACATGATCCATAAAATCGTTCCGCTGATCGCTTATATGAGCAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATggg                                                                                                <  2:166274/251‑1 (MQ=255)                                                                                                                                               ccGCTTTCCGGGTTTATTCCCGCGGCGGAATTTACCGGCGACCCGCAAAATACAACGCTGAGCCTGAGCGTAAACGGCGAACAACGCCAGCAAGGTGCGACTGCGGACATGATCCATAAAATCGTTCCGCTGATCGCTTATATGAGCAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCa                                                                                              <  1:364502/251‑1 (MQ=255)                                                                                                                                                        gggTTTATTCCCGCGGCGGAATTTACCGGCGACCCGCAAAATACAACGCTGAGCCTGAGCGTAAACGGCGAACAACGCCAGCAAGGTGCGACTGCGGACATGATCCATAAAATCGTTCCGCTGATCGCTTATATGAGCAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGAc                                                                                     >  1:250613/1‑251 (MQ=255)                                                                                                                                                                   cgcgGCGGAATTTACCGGCGACCCGCAAAATACAACGCTGAGCCTGAGCGTAAACGGCGAACAACGCCAGCAAGGTGCGACTGCGGACATGATCCATAAAATCGTTCCGCTGATCGCTTATATGAGCAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGGGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGttt                                                                          <  2:304268/251‑1 (MQ=255)                                                                                                                                                                   cgcgGCGGAATTTACCGGCGACCCGCAAAATACAACGCTGAGCCTGAGCGTAAACGGCGAACAACGCCAGCAAGGTGCGACTGCGGACATGATCCATAAAATCGTTCCGCTGATCGCTTATATGAGCAAGTTTGTTTCCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACATTCACTTTCGATGGGCATTCTTTGACATCTCGCGttt                                                                          <  1:298517/251‑1 (MQ=255)                                                                                                                                                                            aTTTACCGGCGACCCGCAAAATACAACGCTGAGCCTGAGCGTAAACGGCGAACAACGCCAGCAAGGTGCGACTGCGGACATGATCCATAAAATCGTTCCGCTGATCGCTTATATGAGCAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACt                                                                 >  1:19150/1‑251 (MQ=255)                                                                                                                                                                                           gCAAAATACAACGCTGAGCCTGAGCGTAAACGGCGAACAACGCCAGCAAGGTGCGACTGCGGACATGATCCATAAAATCGTTCCGCTGATCGCTTATATGAGCAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGaa                                                   >  1:136819/1‑250 (MQ=255)                                                                                                                                                                                            cAAAATGCAACGCTGAGCCTGAGCGTAAACGGCGAACAACGCCAGCAAGGTGCGACTGCGGACATGATCCATAAAATCGTGCCGCTGATCGCTTATATGAGCAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATTGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGCCAACTCGCGTGTTGTAATACTTTTTGCCGCCTGAAAg                                                 <  2:150761/251‑1 (MQ=255)                                                                                                                                                                                                         tgagcctgagcGTAAACGGCGAACAACGCCAGCAAGGTGCGACTGCGGACATGATCCATAAAATCGTTCCGCTGATCGCTTATATGAGCAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACt                                    >  1:277044/1‑251 (MQ=255)                                                                                                                                                                                                                       aaCGGCGAACAACGCCAGCAAGGTGCGACTGCGGACATGATCCATAAAATCGTTCCGCTGATCGCTTATATGAGCAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTTCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGTCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGcc                      <  1:192177/251‑1 (MQ=255)                                                                                                                                                                                                                             gAACAACGCCAGCAAGGTGCGACTGCGGACATGATCCATAAAATCGTTCCGCTGATCGCTTATATGAGCAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTg                <  2:18617/251‑1 (MQ=255)                                                                                                                                                                                                                                      cAGCAAGGTGCGACTGCGGACATGATCCATAAAATCGTTCCGCTGATCGCTTATATGAGCAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAgg       >  1:186794/1‑251 (MQ=255)                                                                                                                                                                                                                                       aGCAAGGTGCGACTGCGGACATGATCCATAAAATCGTTCCGCTGATCGCTTATATGAGCAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGt      <  2:277044/251‑1 (MQ=255)                                                                                                                                                                                                                                       aGCAAGGTGCGACTGCGGACATGATCCATAAAATCGTTCCGCTGATCGCTTATATGAGCAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGt      <  1:288213/251‑1 (MQ=255)                                                                                                                                                                                                                                        gCAAGGTGCGACTGCGGACATGATCCATAAAATCGTTCCGCTGATCGCTTATATGAGCAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTg     <  2:57277/251‑1 (MQ=255)                                                                                                                                                                                                                                           aGGTGCGACTGCGGACATGATCCATAAAATCGTTCCGCTGATCGCTTATATGAGCAAGTTTTTTACCCTCAAGGCCGGTGACTTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACGTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAATGTGCGt  <  2:277222/251‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                       GCGTCAGGCTACGGAAGAGCATGTCCGCAAAGCCATTGCCGGTTATGGCGTGGCGCTCGATCTGACATTGCGTGATGTTCAGGGAAAAATGAAGAAAGCCGGGCAGCCGTGGGAAAAGGCTAAAGCGTTTGATAACTCTTGTCCGCTTTCCGGGTTTATTCCCGCGGCGGAATTTACCGGCGACCCGCAAAATACAACGCTGAGCCTGAGCGTAAACGGCGAACAACGCCAGCAAGGTACGACTGCGGACATGATCCATAAAATCGTTCCGCTGATCGCTTATATGAGCAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGT  >  NC_000913/1228321‑1228805

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑