Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	65,785	A→T	intergenic (‑5/+70)	polB ← / ← araD	DNA polymerase II/L‑ribulose‑5‑phosphate 4‑epimerase AraD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	65,785	0	A	T	100.0%	79.6 / NA	24	intergenic (‑5/+70)	polB/araD	DNA polymerase II/L‑ribulose‑5‑phosphate 4‑epimerase AraD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (11/13);  total (11/13)

GAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGC  >  NC_000913/65561‑66021                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                             gAAAAGCCTTAACCGCCAGCGGGGTCAGGCGAACGCCTGGTTCACCCTGCAAAATATGCGGAGCGCGGGGAACCTGATCGGCGGGAATAACCGCCACGGACTCTGGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGATTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGa                                                                                                                                                                                                                     <  2:338818/250‑1 (MQ=255)             aCGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCATGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGAGCAAGCGGAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCCGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCCGGGTCGGGGTAAGATAAAACCTGCCTGCGCCCCGCTGTAATTCCCTCaaaaaaacacgcgtgattaatgcctggtt                                                                                                                                                                                                        >  1:345019/1‑228 (MQ=255)                                           cACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGt                                                                                                                                                                           <  1:126449/250‑1 (MQ=255)                                                     aaTATGCTGAGGGGGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGccc                                                                                                                                                                <  1:88358/251‑1 (MQ=255)                                                                                 gcggGAATAAACGCCACGGACTCTTGCGTTTCAAGCGTAACCTGCATCGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCTCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGc                                                                                                                                    <  1:196069/251‑1 (MQ=255)                                                                                 gcggGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCCTGATTATAGCCTGGTTTCGTTTGATTGGCTGGGGTTTTATACAGTCATTACTTCCCGTAATATGCCTTCCCGCCATGCTTaagc                                                                                                                                    >  2:330559/1‑248 (MQ=255)                                                                                         aaaCGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAgtg                                                                                                                            >  1:69513/1‑251 (MQ=255)                                                                                           ccGCCACGGACTCTTGCGGGGCAAGCGAAACCGGCACCGGCCCGTTGTCCGCCGCCAGCCAGAAGGAGCCTTCTGTCCCTTGGGGGGTTTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGtt                                                                                                                          <  2:336955/250‑1 (MQ=255)                                                                                           aCGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGtt                                                                                                                          >  2:303328/1‑251 (MQ=255)                                                                                                                 aaGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTcagcag                                                                                                                <  1:148311/239‑1 (MQ=255)                                                                                                                 aaGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGGTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTcagcag                                                                                                                >  2:148311/1‑239 (MQ=255)                                                                                                                           tGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAAACAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATTCGGTAAc                                                                                          >  1:212266/1‑251 (MQ=255)                                                                                                                                                                                ggTGTCCCGCCAGTGTCGGGTTAAGATAAAAACTGCCTGCGCCACGCTGTAATTACATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGGTTGATTGGCTGTGGGTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTTCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCAc                                     >  2:358676/1‑251 (MQ=255)                                                                                                                                                                                     cccGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGCCCTCTTCCAGCACGATg                                 <  2:345019/250‑1 (MQ=255)                                                                                                                                                                                          cAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGGTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTa                           <  2:349080/251‑1 (MQ=255)                                                                                                                                                                                             tgtCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTCTAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGGCATTACTGCCCGTAATATTCCTTCGCGCCATGCTTACGCCGATAGAGGTTATTCAGCAGCGTTTGCTACATATCCGGTAACTGCGGCGCTCACTGCCGGCAGCATATCCCCATATAACCGACCTCTTCCAGcccaatgtctttctgc                        >  2:86522/1‑236 (MQ=255)                                                                                                                                                                                                      aaGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATc                <  1:217094/250‑1 (MQ=255)                                                                                                                                                                                                      aaGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCt               <  1:14811/251‑1 (MQ=255)                                                                                                                                                                                                      aaGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCt               <  1:195480/251‑1 (MQ=255)                                                                                                                                                                                                         agaaaaCCTGCCTGCGCCACGCTGTAATTCCATCACAAAACCAGGCTTGATTTTAGCCTGGTTTCGGGTGATTGGCTGTGGTTTTATACAGTCAGTACTGCCCGTCATATGACTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCATCGGTTGCTGCATATCCGGTAACTGCTGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCGTCCAGCCCGATTGAGTTAGGCACCGCATCTTCg            <  2:353067/249‑1 (MQ=255)                                                                                                                                                                                                            aaaCCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGACATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGc          >  1:242421/1‑250 (MQ=255)                                                                                                                                                                                                                ctgcctgcGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCAtttt     >  1:274976/1‑251 (MQ=255)                                                                                                                                                                                                                  gcctgcGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCAtttt     <  2:236408/249‑1 (MQ=255)                                                                                                                                                                                                                   cctgcGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGc  >  2:95276/1‑251 (MQ=255)                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                             GAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGC  >  NC_000913/65561‑66021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑