Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,177,489	A→G	N57S (AAC→AGC)	lolE →	lipoprotein release complex ‑ inner membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,177,489	0	A	G	100.0%	104.6 / NA	33	N57S (AAC→AGC)	lolE	lipoprotein release complex ‑ inner membrane subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/27);  total (6/27)

GTATGAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGATGGGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCATGGTGTCGCTGATCTCCGTTATTTCTACCATTGGCATTGCCCTCGGCGTGGCGGTATTGATCGTCGGCTTAAGCGCGATGAACGGCTTTGAACGCGAACTGAACAACCGTATTCTGGCGGTGGTGCCGCATGGTGAAATCGAGGCGGTGGATCAGCC‑GTGGACTAACTGGCAGGAAGCACT‑GG‑ATCACGTG‑CAAAAA‑GTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGG  >  NC_000913/1177250‑1177733                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                          gTATGAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGATGGGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCATGGTGTCGCTGATCTCCGTTATTTCTACCATTGGCATTGCCCTCGGCGTGGCGGTATTGATCGTCGGCTTAAGCGCGATGAACGGCTTTGAACGCGAACTGAACAGCCGTATTCTgg                                                                                                                                                                                                                                                <  1:307093/251‑1 (MQ=255)                           gATGGTCGTCTGACGGCGGAACTGAGCCTGATGGGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCATGGTGTCGCTGATCTCCGTTATTTCTACCATTGGCATTGCCCTCGGCGTGGCGGTATTGATCGTCGGCTTAAGCGCGATGAACGGCTTTGAACGCGAACTGAACAGCCGTATTCTGGCGGTGGTGCCGCATGGTGAAATCGAg                                                                                                                                                                                                                      <  1:130883/251‑1 (MQ=255)                                 cgtcTGACGGCGGAACTGAGCCTGATGGGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCATGGTGTCGCTGATCTCCGTTATTTCTACCATTGGCATTGCCCTCGGCGTGGCGGTATTGATCGTCGGCTTAAGCGCGATGAACGGCTTTGAACGCGAACTGAACAGCCGTATTCTGGCGGTGGTGCCGCATGGTGAAATCGAGGCGGTg                                                                                                                                                                                                                <  1:39122/251‑1 (MQ=255)                                  gtcTGACGGCGGAACTGAGCCTGATGGGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCATGGTGTCGCTGATCTCCGTTATTTCTACCATTGGCATTGCCCTCGGCGTGGCGGTATTGATCGTCGGCTTAAGCGCGATGAACGGCTTTGAACGCGAACTGAACAGCCGTATTCTGGCGGTGGTGCCGCATGGTGAAATCGAGGCGGTgg                                                                                                                                                                                                               <  1:57851/251‑1 (MQ=255)                                     ggACGGCGGAACTGAGCCTGATGGGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCATGGTGTCGCTGATCTCCGTTATTTCTACCATTGGCATTGCCCTCGGCGTGGCGGTATTGATCGTCGGCTTAAGCGCGATGAACGGCTTTGAACGCGAACTGAACAGCCGTATTCTGGCGGTGGTGCCGCATGGTGAAATCGAGGCGGTGGATc                                                                                                                                                                                                            <  2:335750/250‑1 (MQ=255)                                       aCGGCGGAACTGAGCCTGATGGGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCATGGTGTCGCTGATCTCCGTTATTTCTACCATTGGCATTGCCCTCGGCGTGGCGGTATTGATCGTCGGCTTAAGCGCGATGAACGGCTTTGAACGCGAACTGAACAGCCGTATTCTGGCGGTGGTGCCGCATGGTGAAATCGAGGCGGTGGATCAg                                                                                                                                                                                                          <  2:129032/251‑1 (MQ=255)                                           cggAACTGAGCCTGATGGGGGCGGAGTAATGGCGATGCCTTTATAGCTATTAATTGGACTGCGTTTTAGTCGAGGACGGCGGCGCGGCGGCATGGTGTCGCTGATCTCCGTTATTTCTACCATTGGCATTGCCCTCGGCGTGGCGGTATTGATCGTCGGCTTAAGCGCGATGAACGGCTTTGAACGCGCACTGAACAGCCGTATTCTGGCAGTGGGGGCGCAAGGGGAAATCGAGGCGGGGGaaacggcgg                                                                                                                                                                                                      >  1:358932/1‑243 (MQ=255)                                                                                ccTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCATGGTGGCGCTGATCTCCGTTATTTCTACCATTGGCATTGCCCTCGGCGTGGCGGTATTGATCGTCGGCTTAAGCGCGATGAACGGCTTTGAACGCGAACTGAACAGCCGTATTCTGGCGGTGGTGCCGCATGGTGAAATCGAGGCGGTGGATCAGCC‑GTGGACTAACTGGCAGGAAGCACT‑GG‑ATCACGTG‑Caaaa                                                                                                                                                             <  1:135392/251‑1 (MQ=255)                                                                                     aTCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCATGGTGTCGCTGATCTCCGTTATTTCTACCATTGGCATTGCCCTCGGCGTGGCGGTATTGATCGTCGGCTTAAGCGCGATGAACGGCTTTGAACGCGAACTGAACAGCCGTATTCTGGCGGTGGTGCCGCATGGTGAAATCGAGGCGGTGGATCAGCC‑GTGGACTAACTGGCAGGAAGCACT‑GG‑ATCACGTG‑CAAAAA‑GTg                                                                                                                                                        <  2:64762/250‑1 (MQ=255)                                                                                              aaTTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCATGGTGTCGCTGATCTCCGTTATTTCTACCATTGGCATGGCCCTCGGCGTGGCGGTATTGATCGTCGGCTTAAGCGCGATGAACGGCTTTGAACGCGAACTGAACAGCCGTATTCTGGCGGTGGTGCCGCATGGTGAAATCGAGGCGGTGGATCAGCC‑GTGGACTAACTGGCAGGAAGCACT‑GG‑ATCACGTG‑CAAAAA‑GTGCCGGGTATtg                                                                                                                                              <  2:335242/251‑1 (MQ=255)                                                                                                 tGGCCTGCGTTTTAGTGGCGGACGGCGGCGCGGCGGCATGGTGTCGCTGATCTCCGTTATTTCTACCAGTGGCATTGCCCTCGGCGTGGCGGTATTGATCGTCGGCTTAAGCGCGATGAACGGCTTTGAACGCGAACTGAACAGCCGTATTCTGGCGGTGGTGCCGCATGGTGAAATCGAGGCGGTGGATCAGCC‑GTGGACTAACTGGCAGGAAGCACT‑GG‑ATCACGTG‑CAAAAA‑GTGCCGGGTATtgcc                                                                                                                                            <  2:118452/250‑1 (MQ=255)                                                                                                            ttAGTCGCGGACGGCGGCGCGGCGGCATGGTGTCGCTGATCTCCGTTATTTCTACCATGGGCATTGCCCTCGGCGTGGCGGTATTGATCGTCGGCTTAAGCGCGATGAACGGCTTTGAACGCGAACTGAACAGCCGTATTCTGGCGGTGGTGCCGCATGGTGAAATCGAGGCGGTGGATCAGCC‑GTGGACTAACTGGCAGGAAGCACT‑GG‑ATCACGTG‑CAAAAA‑GTGCCGGGTATTGCCGCTGCCGCGcc                                                                                                                                 <  2:49007/250‑1 (MQ=255)                                                                                                             tAGTCGCGGACGGCGGCGCGGCGGCATGGTGTCGCTGATCTCCGTTATTTCTACCATTGGCATTGCCCTCGGCGTGGCGGTATTGATCGTCGGCTTAAGCGCGATGAACGGCTTTGAACGCGAACTGAACAGCCGTATTCTGGCGGTGGTGCCGCATGGTGAAATCGAGGCGGTGGATCAGCC‑GTGGACTAACTGGCAGGAAGCACT‑GG‑ATCACGTG‑CAAAAA‑GTGCCGGGTATTGCCGCTGCCGCGCCg                                                                                                                                <  2:84361/250‑1 (MQ=255)                                                                                                              aGTCGCGGACGGCGGCGCGGCGGCATGGTGTCGCTGCTCTCCGTTCTTTCTACCATTGGCATTGCCCTAGGCGTGGCGGCAATGATCGTCGGCTTAAGCGCGATGAACGGCTTTGAACGCGAACTGAACAGCCGTATTCTGGCGGTGGTGCCGCATGGTGAAATCGAGGCGGTGGGACAGCC‑GGGGACTAAATGGGCGGGAGCACTTGG‑AAC‑CGTGGCAAAAA‑GGGCGGGGCTTTGCCG‑TGCCGCGCCgg                                                                                                                               >  1:213259/1‑249 (MQ=255)                                                                                                                    ggACGGCGGCGCGGCGGCATGGTGTCGCTGATCTCCGTTATTTCTACCATTGGCATTGCCCTCGGCGTGGCGGTATTGATCGTCGGCTTAAGCGCGATGAACGGCTTTGAACGCTAACTGAACAGCCGTATTCTGGCGGTGGTGCCGCATGGTGAAATCGAGGCGGTGGATCAGCC‑GTGGACTAACTGGCAGGAAGCACT‑GG‑ATCACGTG‑CAAAAA‑GTGCCGGGTATTGCCGCTGCCGCGCCGTATATCaa                                                                                                                        <  1:45079/251‑1 (MQ=255)                                                                                                                                ggcggcATGGTGTCGCTGATCTCCGTTATTTCTACCATTGGCATTGCCCTCGGCGTGGCGGTATTGATCGTCGGCTTAAGCGCGATGAACGGCTTTGAACGCGAACTGAACAGCCGTATTCTGGCGGTGGTGCCGCATGGTGAAATCGAGGCGGTGGATCAGCC‑GTGGACTAACTGGCAGGAAGCACT‑GG‑ATCACGTG‑CAAAAA‑GTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCt                                                                                                            <  1:78191/251‑1 (MQ=255)                                                                                                                                   ggcATGGTGTCGCTGATCTCCGTTATTTCTACCATTGGCATTGCCCTCGGCGTGGCGGTATTGATCGTCGGCTTAAGCGCGATGAACGGCTTTGAACGCGAACTGAACAGCCGTATTCTGGCGGTGGTGCCGCATGGTGAAATCGAGGCGGTGGATCAGCC‑GTGGACTAACTGGCAGGAAGCACT‑GG‑ATCACGTG‑CAAAAA‑GTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCtggt                                                                                                         <  1:207871/251‑1 (MQ=255)                                                                                                                                                tGATCTCCGTTATTTCTACCATTGGCATTGCCCTCGGCGTGGCGGTATTGATCGTCGGCTTAAGCGCGATGAACGGCTTTGAACGCGAACTGAACAGCCGTATTCTGGCGGGGGTGCCGCATGGGGGAATCGAGGCGGGGGGTCAGCCCGGGGCCTA‑CTGGCGGGGAGCACT‑GGGTTCA‑GTG‑CAAAAAAGGGCCGGGAATT‑CCGCCGCCCCGCCGGCTATCAAtttttccggggttggggaaaggggggcg                                                                                            >  1:425/1‑226 (MQ=255)                                                                                                                                                      ccGTTATTTCTACCATTGGCATTGCCCTCGGCGTGGCGGTATTGATCGTCGGCTTAAGCGCGATGAACGGCTTTGAACGCGAACTGAACAGCCGTATTCTGGCGGTGGTGCCGCATGGTGAAATCGAGGCGGTGGATCAGCC‑GTGGACTAACTGGCAGGAAGCACT‑GG‑ATCACGTG‑CAAAAA‑GTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCtt                                                                                      <  2:316153/251‑1 (MQ=255)                                                                                                                                                        gTTATTTCTACCATTGGCATTGCCCTCGGCGTGGCGGTATTGATCGTCGGCTTAAGCGCGATGAACGGCTTTGAACGCGAACTGAACAGCCGTATTCTGGCGGTGGTGCCGCATGGTGAAATCGAGGCGGTGGATCAGCC‑GTGGACTAACTGGCAGGAAGCACT‑GG‑ATCACGTG‑CAAAAA‑GTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTcg                                                                                    <  1:361609/251‑1 (MQ=255)                                                                                                                                                          tATTTCTAACATTGGCATTGCCCTCGGCGTGGCGGTATTGATCGTCGGCTTAAGCGCGATGAACGGCTTTGAACGCGAACTGAACAGCCGTATTCTGGCGGTGGTGCCGCATGGTGAAATCGAGGCGGGGGGTCAGCC‑GGGGGCTAACTGGCCGGAAGCACT‑GG‑GTCACGTG‑CCAAAA‑GGGCCCGGTATTGCCGCTGCCGCGCCGGATATCAATTTCACCGGGCTGGTGGGAAGGGGCGCGGATATTcgcg                                                                                  >  1:303400/1‑251 (MQ=255)                                                                                                                                                                 aCCATTGGCATTGCCCTCGGCGTGGCGGTATTGATCGTCGGCTTAAGCGCGATGAACGGCTTTGAACGCGAACTGAACAGCCGTATTCTGGCGGTGGTGCCGCATGGTGAAATCGAGGCGGTGGATCAGCC‑GTGGACTAACTGGCAGGAAGCACT‑GG‑ATCACGTG‑CAAAAA‑GTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTTGTGGAAAGTGGCGCGAATCTTCGCGCAAt                                                                              <  1:313167/248‑1 (MQ=255)                                                                                                                                                                       ggCATTGCCCTCGGCGTGGCGGTATTGATCGTCGGTTTAAGCGCGATGAACGGCTTTGAACGCGAACTGAACAGCCGTATTCTGGCGGTGGTGCCGCATGGTGAAATCGAGGCGGTGGATCAGCC‑GTGGACTAACTGGCAGGAAGCACT‑GG‑ATCACGTG‑CAAAAA‑GTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGa                                                                      <  2:357756/250‑1 (MQ=255)                                                                                                                                                                                cgaggCGTTGCGGTAATGATCGGCGGCATAAGCGCGATGAAGGCCTTTGAACGCGAACTGAACAGCCGTATTCTGTCGGTGGTGCCGCATGGTGAAATCGAGGCGGTGGATCAGCC‑GTGGACTAACTGGCAGGAAGCACT‑GG‑ATCACTTG‑CAAGAA‑GGGCCGGGTGTTGGCGCTGGCGCGCCGTATATCAATTTCGCCGGGGTGGTGGAAAGTTGGTCGAATCGTCGCTCAATCCAGTTGATGGGCGTTaa                                                            <  2:245992/248‑1 (MQ=255)                                                                                                                                                                                           ggTATTGATCGTCGGCTGAAGCGCGATGAGCGGCTTTTAACGCGAACTGAACAGCCGTATTCTGGCGGTGGTGCCGCATGGTGAAATCGAGGCGGTGGATCAGCC‑GTGGACTAACTGGCAGGAAGCACT‑GG‑ATCACGTG‑CAAAAA‑GTGCCGGGTATTGCCGCTGCCGCGCCGTATATCGATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCATGTGAAGGGCGTGAACCCGCAACAgg                                                 <  2:349612/251‑1 (MQ=255)                                                                                                                                                                                            gTATTGATCGTCGGCTTAAGCGCGATGAACGGCTTTGAACGCGAACTGAACAGCCGTATTCTGGCGGTGGTGCCGCATGGTGAAATCGAGGCGGTGGATCAGCC‑GTGGACTAACTGGCAGGAAGCACT‑GG‑ATCACGTG‑CAAAAA‑GTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGa                                                <  1:328440/251‑1 (MQ=255)                                                                                                                                                                                                       cgGCTTAAGCGCGATGAACGGCTTTGAACGCGAACTGAACAGCCGTATTCTGGCGGTGGTGCCGCATGGTGAAATCGAGGCGGTGGATCAGCC‑GTGGACTAACTGGCAGGAAGCACT‑GG‑ATCACGTG‑CAAAAA‑GTGCCGGGTATtgcc                                                                                                                                            <  2:179266/148‑1 (MQ=255)                                                                                                                                                                                                       cgGCTTAAGCGCGATGAACGGCTTTGAACGCGAACTGAACAGCCGTATTCTGGCGGTGGTGCCGCATGGTGAAATCGAGGCGGTGGATCAGCC‑GTGGACTAACTGGCAGGAAGCACT‑GG‑ATCACGTG‑CAAAAA‑GTGCCGGGTATtgcc                                                                                                                                            >  1:179266/1‑148 (MQ=255)                                                                                                                                                                                                              aGCGCGATGAACGGCTTTGAACGCGAACTGAACAGCCGTATTCTGGCGGTGGTGCCGCATGGTGAAATCGAGGCGGTGGATCATCC‑GTGGACTAACTGGCAGGAAGCACT‑GG‑ATCACGTG‑CAAAAA‑GTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCAtt                              <  1:1302/251‑1 (MQ=255)                                                                                                                                                                                                                                    gcgAACGGAACAGCCGTATTCTGGCGGTGGTGCCGCATGGTGAAATCGAGGCGGTGGATCAGCC‑GTGGACTAACTGGCAGGAAGCACT‑GG‑ATCACGTG‑CAAAAA‑GTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGAt        <  2:347658/251‑1 (MQ=255)                                                                                                                                                                                                                                       aaCTGAACAGCCGTATTCTGGCGGTGGTGCCGCATGGTGAAATCGAGGCGGTGGATCAGCC‑GTGGACTAACTGGCAGGAAGCACT‑GG‑ATCACGTG‑CAAAAA‑GTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCg     <  2:303400/251‑1 (MQ=255)                                                                                                                                                                                                                                       aaCTGAACAGCCGTATTCTGGCGGTGGTGCCGCATGGTGAAATCGAGGCGGTGGATCAGCC‑GTGGACTAAATGGCCGGAAGCACT‑GG‑ATACCGTG‑AAAAAA‑GGGCCGGGGATTGCCGCCGCCGCGCCGTATATCAATTTCACCGGGGTGGGGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGGAGGGCGTTCACCCGCAACAGGAACAGCGTCTGCGCGCATTACCCTCCGTTGTCCACGGCGAAGCg     >  2:344658/1‑251 (MQ=255)                                                                                                                                                                                                                                           gAACAGCCGTATTCTGGCGGTGGTGCCGCATGGTGAAATCGAGGCGGTGGATCAGCC‑GTGGACTAACTGGCAGGAAGCACT‑GG‑ATCACGTG‑CAAAAA‑GTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTgg  <  1:92515/250‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                          GTATGAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGATGGGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCATGGTGTCGCTGATCTCCGTTATTTCTACCATTGGCATTGCCCTCGGCGTGGCGGTATTGATCGTCGGCTTAAGCGCGATGAACGGCTTTGAACGCGAACTGAACAACCGTATTCTGGCGGTGGTGCCGCATGGTGAAATCGAGGCGGTGGATCAGCC‑GTGGACTAACTGGCAGGAAGCACT‑GG‑ATCACGTG‑CAAAAA‑GTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGG  >  NC_000913/1177250‑1177733

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑