Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,104,141	G→A	G64D (GGC→GAC)	csgB →	curlin, minor subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,104,141	0	G	A	100.0%	33.8 / NA	14	G64D (GGC→GAC)	csgB	curlin, minor subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/6);  total (8/6)

TAAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATACTGGGTGCGCCTGGGATTGCAGCCGCAGCAGGTTATGATTTAGCTAATTCAGAATATAACTTCGCGGTAAATGAATTGAGTAAGTCTTCATTTAATCAGGCAGCCATAATTGGTCAAGCTGGGACTAATAATAGTGCTCAGTTACGGCAGGGAGGCTCAAAACTTTTGGCGGTTGTTGCGCAAG‑AAGGTAGTAGCAACCGGGCAAAGATTGACCAGACAGGAGATTATAA‑CCTTGCATATATTGATCAGGCGGGCAGTGCCAACGATGCCAGTATTTCGCAAGGTGCTTATGGTAATACTGCGATGATTAT‑CCAGAAAGGTTCTGGTAATAAAGCAAATATTACACAGTATGGTACTCAAAAAACGGCAATTGTAGTGCAGAGACAG  >  NC_000913/1103921‑1104373                                                                                                                                                                                                                             |                                                                                                                                                                                                                                            tAAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATACTGGGTGCGCCTGGGATTGCAGCCGCAGCAGGTTATGATTTAGCTAATTCAGAATATAACTTCGCGGTAAATGAATTGAGTAAGTCTTCATTTAATCAGGCAGCCATAATTGGTCAAGCTGGGACTAATAATAGTGCTCAGTTACGGCAGGGAGACTCAAAACTTTTGGCGTTTGTTGCGCa                                                                                                                                                                                                                  <  2:358296/248‑1 (MQ=255) tAAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATACTGGGTGCGCCTGGGATTGCAGCCGCAGCAGGTTATGATTTAGCTAATTCAGAATATAACTTCGCGGTAAATGAATTGAGTAAGTCTTCATTTAATCAGGCAGCCATAATTGGTCAAGCTGGGACTAATAATAGTGCTCAGTTACGGCAGGGAGACTCAAAACTTTTGGCGGTTGTTGCGCa                                                                                                                                                                                                                  >  1:291813/1‑248 (MQ=255)                            aacaTGAAAAACAAATTGTTATTTATGATGTTAACAATACTGGGTGCGCCTGGGATTGCAGCCGCAGCAGGTTATGATTTAGCTAATTCAGAATATAACTTCGCGGTAAATGAATTGAGTAAGTCTTCATTTAATCAGGCAGCCATAATTGGTCAAGCTGGGACTAATAATAGTGCTCAGTTACGGCAGGGAGACTCAAAACTTTTTGCGGGTTTTGCGCAAG‑AAGGGAGTAGCAAACGGGGCAAGATTg                                                                                                                                                                                    >  2:13959/1‑250 (MQ=255)                                tGAAAAACAAATTGTTATTTATGATGTTAACAATACTGGGTGCGCCTGGGATTGCAGCCGCAGCAGGTTATGATTTGGCTAATTCAGAATATAACTTCGCGGTAAATGAATTGAGTAAGTCTTCATTTAATCAGGCAGCCATAATTGGTCAAGCTGGGACTAATAATAGTGCTCAGTTACGGCAGGGAGACTCAAAACTTTTTGCGGTTTTTTCGCAAGCAAGG‑AGTAGCAAACGGGCAAAAATTGAcc                                                                                                                                                                                 >  2:185559/1‑249 (MQ=255)                                                              cAATCCTGGGTGCGCCTGGGATTGCAGCCGCAGCAGGTTATGATTTAGCTAATTCAGAATATAACTTCGCGGTAAATGAATTGAGTAAGTCTTCATTTAATCAGGCAGCCATAATTGGTCAAGCTGGGACTAATAATAGTGCTCAGTTACGGCAGGGAGAACCAAAACCTTTGGCGGGTTTTTCGCAAG‑AAGGTAGTAGCAACCGGGCAAAGATTTGCCAGACAGGGGATTATAA‑ACTTTCatatttttat                                                                                                                                                >  2:294682/1‑245 (MQ=255)                                                                            ccTGGGATTGCAGCCGCAGCAGGTTATGATTTAGCTAATTCAGAATATAACTTCGCGGTAAATGAATTGAGTAAGTCTTCATTTAATCAGGCAGCCATAATTGGTCAAGCTGGGACTAATAATAGTGCTCAGTTACGGCAGGGAGACTCAAAACTTTTGGCGGTTGTTGCGCAAG‑AAGGTAGTAGCAACCGGGCAAAGATTGACCAGACAGGAGATTATAA‑CCTTGCATATATTGATCAGGCGGGCAGTGc                                                                                                                                  <  2:269940/251‑1 (MQ=255)                                                                                      cAGCCGCAGCAGGTTATGATTTAGCTAATTCAGAATATAACTTCGCGGTAAATGAATTGAGTAAGTCTTCATTTAATCAGGCAGCCATAATTGGTCAAGCTGGGACTAATAATAGTGCTCAGTTACGGCAGGGAGACTCAAAACTTTTGGCGGCTGTTGCGCAAG‑AAAGGAGTAGCAAACGGGCAAAGAATGACCAAACAGGAGATTATAAACCTT‑CCTAAAATGATCAAGCGGGCAGGGCCaaagaagcc                                                                                                                        >  1:156996/1‑244 (MQ=255)                                                                                                                                tCGCGGTAAATGAATTGAGTAAGTCTTCATTTAATCAGGCAGCCATAATTGGTCAAGCTGGGACTAATAATAGTGCTCAGTTACGGCAGGGAGACTCAAAACTTTTGGCGGTTGTTGCGCAAG‑AGGGTAGTAGCAACCGGGCAAAGATTGACCAGACAGGAGATTATAA‑CCTTGCATATATTGATCAGGCGGGCAGTGCCAACGATGCCAGTATTTCGCAAGGTGCTTATGGTAATACTGCGATGATTa                                                                                <  2:257374/249‑1 (MQ=255)                                                                                                                                                            aTTTAATCAGGCAGCCATAATTGGTCAAGCTGGGACTAATAATAGTGCTCAGTTACGGCAGGGAGACTCAAAACTTTTTGCGGTTGTTGCGCCAG‑AAGGGAGTAGCAACCCGGCAAAGATTGACCAGACAGGGGATTATAA‑CCTTGCATATATTTATCAGGCGGGCAGGGGCAACGGAGCCCGTATTTTGCAAGGTGCTTTTGGTAATTCTGCGCAGGTTATTCCA‑AAAGGTTCTGGgaattaaaacaaa                                                  >  2:240063/1‑241 (MQ=255)                                                                                                                                                                aaTCAGGCAGCCATAATTGGTCAAGCTGGGACTAATAATAGTGCTCAGTTACGGCAGGGAGACTCCAAAATTTTGGCGGTTGTTGCGCAAG‑AAGGGAGTAGCAACCGGGCAAAGATTGACCAGACCGGAGATTATAA‑CCTTGCATATATTGATCAGGCGGGCAGTGCCAACg                                                                                                                             >  1:369726/1‑172 (MQ=255)                                                                                                                                                                aaTCAGGCAGCCATAATTGGTCAAGCTGGGACTAATAATAGTGCTCAGTTACGGCAGGGAGACTCAAAACTTTTGGCGGTTGTTGCGCAAG‑AAGGTAGTAGCAACCGGGCAAAGATTGACCAGACAGGAGATTATAA‑CCTTGCATATATTGATCAGGCGGGCAGTGCCAACg                                                                                                                             <  2:369726/172‑1 (MQ=255)                                                                                                                                                                aaTCAGGCAGCCATAATTGGTCAAGCTGGGACTAATAATAGTGCTCAGTTACGGCAGGGAGACTCAAAACTTTTGGCGGTTGTTGCGCAAG‑AAGGTAGTAGCAACCGGGCAAAGATTGACCAGACAGGAGATTATAA‑CCTTGCATATATTGATCAGGCGGGCAGTGCCAACGATGCCAGTATTTCGCAAGGTGCTTATGGTAATACTGCGATGATTAT‑CCAGAAAGGTTCTGGTAATAAAGCAAATATTa                                              <  1:142875/250‑1 (MQ=255)                                                                                                                                                                                                                      cAGGGAGACTCAAAACTTTTGGCGGTTGTTGCGCAAG‑AAGGTAGTAGCAACCGGGCAAAGATTGACCAGACAGGAGATTATAA‑CCTTGCATATATTGATCAGGCGGGCAGTGCCAACGATGCCAGTATTTCGCAAGGTGCTTATGGTAATACTGCGATGATTAT‑CCAGAAAGGTTCTGGTAATAAAGCAAATATTACACAGTATGGTACTCAAAAAACGGCAATTGTAGTGCAGAGACAg  >  1:186962/1‑240 (MQ=255)                                                                                                                                                                                                                      cAGGGAGACTCAAAACTTTTGGCGGTTGTTGCGCAAG‑AAGGTAGTAGCAACCGGGCAAAGATTGACCAGACAGGAGATTATAA‑CCTTGCATATATTGATCAGGCGGGCAGTGCCAACGATGCCAGTATTTCGCAAGGTGCTTATGGTAATACTGCGATGATTAC‑CCAGAAAGGTTCTGGTAATAAAGCAAATATTACACAGTATGGTACTCAAAAAACGGCAATTGTAGTGCAGAGACAg  <  2:186962/240‑1 (MQ=255)                                                                                                                                                                                                                             |                                                                                                                                                                                                                                            TAAATGTACGACCAGGTCCAGGGTGACAACATGAAAAACAAATTGTTATTTATGATGTTAACAATACTGGGTGCGCCTGGGATTGCAGCCGCAGCAGGTTATGATTTAGCTAATTCAGAATATAACTTCGCGGTAAATGAATTGAGTAAGTCTTCATTTAATCAGGCAGCCATAATTGGTCAAGCTGGGACTAATAATAGTGCTCAGTTACGGCAGGGAGGCTCAAAACTTTTGGCGGTTGTTGCGCAAG‑AAGGTAGTAGCAACCGGGCAAAGATTGACCAGACAGGAGATTATAA‑CCTTGCATATATTGATCAGGCGGGCAGTGCCAACGATGCCAGTATTTCGCAAGGTGCTTATGGTAATACTGCGATGATTAT‑CCAGAAAGGTTCTGGTAATAAAGCAAATATTACACAGTATGGTACTCAAAAAACGGCAATTGTAGTGCAGAGACAG  >  NC_000913/1103921‑1104373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑