Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	968,837	G→A	D158N (GAT→AAT)	lpxK →	tetraacyldisaccharide 4'‑kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	968,837	0	G	A	100.0%	103.6 / NA	32	D158N (GAT→AAT)	lpxK	tetraacyldisaccharide 4'‑kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/16);  total (16/16)

TCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGT‑AACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTGATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGG‑CCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGTG‑TCCCTCGC‑AGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGATTGG  >  NC_000913/968596‑969081                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                        tCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGT‑AACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGaa                                                                                                                                                                                                                                                 <  1:232222/250‑1 (MQ=255)  cgcgTGGGGGTCGTATCGCGGGGATATGGTGGTAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGATATGAAACTGTGTTGATTTATTAAACCACGCATGCGCCCGTTGCGGGTTCTCCCGCTCGTTCTGGTGCGGGAAAAGCCATTCTTGCGCCACACCCTGATGGGCAAATCCTCGTCAACC‑ACGACGGTTTACAGCACTAACGTCTGGCGCTTATTGTGGAAAt                                                                                                                                                                                                                                               >  2:295439/1‑251 (MQ=255)   gcgTGGGGGTCGTATCGCGGGGATATGGTGGTAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGT‑AACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAAtt                                                                                                                                                                                                                                              <  1:117201/251‑1 (MQ=255)        ggggTCGTATCGCGGGGATATGGTGGTAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGT‑AACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGt                                                                                                                                                                                                                                         <  1:121062/251‑1 (MQ=255)        ggggTCGTATCGCGGGGATATGGTGGTAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCAATGATGCGCCTGTTGCGGGTTCTCCCGTTCGTTCTGATGCGGTAAAAACCATTCTGGCGCAACACCCTGATGTGCAGATCCTCGT‑AACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGGAATTGTCGt                                                                                                                                                                                                                                         >  1:360654/1‑251 (MQ=255)            tCGTATCGCGGGGATATGGTGGTAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGGGATGAACCTGTGTTGGTTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTTGTTTTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGGTGTGGCGATCATCGT‑AACCGACGACGGTTTATAGCATTACCGTTTGGCCCGTAATGTGGGAATTGTTGTTAtt                                                                                                                                                                                                                                     >  2:218242/1‑251 (MQ=255)                       ggATATGGTGGTAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGT‑AACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCg                                                                                                                                                                                                                          <  2:27964/251‑1 (MQ=255)                                ggtAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGT‑AACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTg                                                                                                                                                                                                                  <  1:148805/250‑1 (MQ=255)                                               tATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTTTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGT‑AACCGACGACGGGTTACAGCATTAACGTCTGGCGCGTAATTTGGAAATTGTCGTTATTGATGGGGTGCGGCGCTTTGGCAATGGCTGGGGGtt                                                                                                                                                                                                  >  1:300604/1‑251 (MQ=255)                                                                   aGATACCACAACAGCACAGGCGGGGGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGT‑AACCGACGACGGGTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGGCGCTTTGGCAATGGCTGGGGGGTGCCGGCGGGGGCCAAtcggg                                                                                                                                                                              >  1:269704/1‑247 (MQ=255)                                                                          acaacaGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGT‑AACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGG‑CCAATGCGTgagcgagc                                                                                                                                                                      <  1:89585/251‑1 (MQ=255)                                                                                     ggCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGT‑AACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGG‑GCCATGCGGgagcgggc                                                                                                                                                                      >  1:14941/1‑237 (MQ=255)                                                                                     ggCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGT‑AACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGG‑CCAATGCGTgagcgagc                                                                                                                                                                      <  2:14941/240‑1 (MQ=255)                                                                                                   cTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGT‑AACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGG‑CCAATGCGTGAGCGAGGGGGGCGCTTTAAGTCGGTTGGTGCg                                                                                                                                             >  2:165716/1‑251 (MQ=255)                                                                                                          gATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGT‑AACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGG‑CCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCg                                                                                                                                      <  1:86718/251‑1 (MQ=255)                                                                                                               aTCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGGTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGT‑AACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGG‑CCAATGCGTGAGCGAGCGGTGCGCTTAAATTCGGTTGATGCGGTAAGCGTCaa                                                                                                                                  <  2:246379/250‑1 (MQ=255)                                                                                                                  aaCGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGT‑AACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGG‑CCAATGCGTGAGCGAGCGGGGGGCTTAAAGTCGGTTGATGCGGGAATCGTCAAcggc                                                                                                                              >  1:325385/1‑251 (MQ=255)                                                                                                                  aaCGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGT‑AACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGG‑CCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAAcggc                                                                                                                              <  1:218242/251‑1 (MQ=255)                                                                                                                       aCTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGT‑AACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGG‑GCAATGCGTTGGCGGGGGGGGGGCTTAAAGTCGGTTGATGGGGGAATCGTCaaagggggggg                                                                                                                         >  1:14439/1‑242 (MQ=255)                                                                                                                           aTGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGT‑AACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGG‑CCAATGCGTGGGCGGGCGGGGCGCTTAAAGTCGGTTGGTGGGGGAATCGGCAAcgcgggggtggcc                                                                                                                     >  2:201758/1‑240 (MQ=255)                                                                                                                                                        tcTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGT‑AACCGAAGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGG‑CCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGTG‑TCCCTCGC‑AGCGGTGAAATCCCCATGCATctgc                                                                                       <  1:152686/250‑1 (MQ=255)                                                                                                                                                               cGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGT‑AACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGG‑CCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGGG‑GCCCCCGC‑AGCGGGGAAATccccctccctcttcttcccggg                                                                               >  1:153270/1‑233 (MQ=255)                                                                                                                                                                ggTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGT‑AACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGG‑CCCATGCGTGAGCGAGCGGGGCGCTTAAAGGCGGTTGATGCGGTAATCGGCAACGGCGGGG‑TCCCTCGC‑CGCGGTGAAATCCCCCTGCATCTGCTGCCggggc                                                                              >  1:82951/1‑249 (MQ=255)                                                                                                                                                                     aaGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGT‑AACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGG‑CCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGTG‑TCCCTCGC‑AGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCg                                                                         <  2:300604/251‑1 (MQ=255)                                                                                                                                                                               ggCGCAACACCCTGATGTGCAGATCATCGT‑AACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGG‑CCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGTCGGTG‑TCCCTCGC‑AGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAAttt                                                                 <  1:234081/249‑1 (MQ=255)                                                                                                                                                                                     acacCCTGATGTGCAGATCATCGT‑AACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGCAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGG‑CCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTTATTCGGTAATCGTCCACGGCGGTG‑TCCCCCCC‑CGCTGGGAAATCCCCCTGCATCCTCTTCCGGGTCCAGCGGTTAATTTACGTAccc                                                         >  1:2348/1‑250 (MQ=255)                                                                                                                                                                                        cccTGATGTGCAGATCATCGT‑AACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGG‑CCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGTG‑TCCCTCGC‑AGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGTa                                                      <  2:170400/251‑1 (MQ=255)                                                                                                                                                                                            gATGTGCAGATCATCGT‑AACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGG‑CCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGGTCCC‑CCC‑CGCGGTGGAAACCCCCTGCATCCGCCGCCGGGGCAGGCGGGGGATTTTCCTACCCGTAcgcg                                                  >  2:19719/1‑251 (MQ=255)                                                                                                                                                                                                tgCAGATCATCGT‑AACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGG‑GCAAAGCGGGAGCGAGCGGGGCGCTTAAAGTCGGGTGATGGGGGAATCGTCAACGGCGGGG‑TTCCCCGCCAG‑GGGGAAATCCCCCCGCATTTGCTGCCGGGTCAGGCGGGGGGTTTATGTACCggggcgcggtgg                                              >  1:182207/1‑241 (MQ=255)                                                                                                                                                                                                       catcGT‑AACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGG‑CCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGTG‑TCCCTCGC‑AGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTg                                       <  2:69544/251‑1 (MQ=255)                                                                                                                                                                                                                       cgGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGG‑CCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGTG‑TCCCTCGC‑AGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATg                        <  1:328460/251‑1 (MQ=255)                                                                                                                                                                                                                                             gcgcGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGG‑CCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGGG‑TCCCTCGC‑AGCGGGGGAATCCCCAAGCATCTGCTGCCGGGGCAGGGGGGGAATTTTCGTACCGGGACGCGGTGTGAAGTTGCTCAGCTTGAACATGGAGTGGCGGTGgggggggtttg  >  1:93542/1‑246 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                        TCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGT‑AACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTGATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGG‑CCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGTG‑TCCCTCGC‑AGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGATTGG  >  NC_000913/968596‑969081

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑