Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	827,819	A→G	V388A (GTG→GCG)	ybhF ←	ABC exporter ATP binding subunit YbhF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	827,819	0	A	G	100.0%	109.6 / NA	34	V388A (GTG→GCG)	ybhF	ABC exporter ATP binding subunit YbhF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/13);  total (21/13)

GTTTAAAACCTAATGGCAGTTCATCGGTGGCGTGGGAGGCGATACTTTTCAGGCCGAACGCCTCGCTCATGCGGGAGATTTTTTCGTTCTGCGCCCGACCGCGTAAGCCATACACACCAGAGAAAAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAACTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCAAACCAAAAATCTCCCCACGTTTAACGGCAAAGTTGACGTGATCGGTGGCGGCAAAATCCCCAAATTTCTTGGTCAGTTCTTTCGCTTCGATCACCGTCTCGCCGGGTGTGCCTTCTACCGTATGTAATATTGCGCCCAGCGGCGATTCCGAGGTTCCGGC  >  NC_000913/827583‑828063                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                     gTTTAAAACCTAATGGCAGTTCATCGGTGGCGTGGGAGGCGATACTTTTCAGGCCGAACGCCTCGCTCATGCGGGAGATTTTTTCGTTCTGCGCCCGACCGCGTAAGCCATACACACCAGAGAAAAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAACTCTCTTTCAGATCCATCCCCAGCGCCAGCGCCTGGCCg                                                                                                                                                                                                                                        <  1:179112/251‑1 (MQ=255)                          ggTGGCGTGGGAGGCGATACTTTTCAGGCCGAACGCCTCGCTCATGCGGGAGATTTTTTCGTTCTGCGCCAGGCCGCGTAAGCCATACACACCAGAGAAAAAGCGTAAATTCTGTTCGACCGTCATGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAACTCTCTTTCAGATCCATCCCCAGCGCCAGCGGCTGGCCGGAAGTCGGCACCAGCAAACCGcaca                                                                                                                                                                                                               <  2:204192/251‑1 (MQ=255)                                gTGGGAGGCGATACTTTTCAGGCCGAACGCCTCGCTCATGCGGGCGATTTTTTCGTTCTGCGCCCGACCGCGTAAGCCATACACACCAGAGAAAAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAACTCTCTTTCAGATCCATCCCCAGCGCCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCt                                                                                                                                                                                                         >  1:45036/1‑251 (MQ=255)                                      ggCGATACTTTTCAGGCCGACCGCCTCGCTCATGCGGGAGCTTTTTTCGTTCTGCGCCCGAACGCGTAAGCCATCCACACCAGAGACAAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGCACTCTCTTTCAGATCCATCCCCAGCGCCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATAATCTTAAAgg                                                                                                                                                                                                   >  2:348407/1‑251 (MQ=255)                                                     gCCGAACGCCTCGCTCATGCGGGAGATTTTTTCGTTCTGCGCCCGACCGCGTAAGCCATACACACCAGAGAAAAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAACTCTCTTTCAGATCCATCCCCAGCGCCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTACCcg                                                                                                                                                                                    >  2:307524/1‑251 (MQ=255)                                                                cGCTCATGCGGGAGATTTTTTCGTTCTGCGCCCGACCGCGTAAGCCATACACACCAGAGAAAAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAACTCTCTTTCAGATCCATCCCCAGCGCCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGc                                                                                                                                                                         <  1:262519/251‑1 (MQ=255)                                                                                tttttCGTTCTGCGCCCGACCGCGTAAGCCATACACACCAGAGAAAAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAACTCTCTTTCAGATCCATCCCCAGCGCCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCAAACCaaaa                                                                                                                                                          <  1:303923/250‑1 (MQ=255)                                                                                tttttCGTTCTGCGCCCGACCGCGTAAGCCATACACACCAGAGAAAAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAAGTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAACTCTCTTTCAGATCCATCCCCAGCGCCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTCGCCCCAGCAAACCaaaa                                                                                                                                                          <  2:14974/250‑1 (MQ=255)                                                                                  tttCGTTCTGCGCCCGACCGCGTAAGCCATACACACCAGAGAAAAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAACTCTCTTTCAGATCCATCCCCAGCGCCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCAAACCAAAAAtc                                                                                                                                                       >  1:341776/1‑251 (MQ=255)                                                                                   ttCGTTCTGCGCCCGACCGCGTAAGCCATACACACCAGAGAAAAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAACTCTCTTTCAGATCCATCCCCAGCGCCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCAAACCAAAActct                                                                                                                                                      >  1:141924/1‑251 (MQ=255)                                                                                         cTGCGCCCGACCGCGTAAGCCATACACACCAGCGAAAAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGATATGCTGGCGCGCTTTACCGGAACTCTCTTTCAGATCCATCCCCAGCGCCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCAAACCAAAAATCTCCCCAc                                                                                                                                                >  2:166529/1‑251 (MQ=255)                                                                                                     gcgTAAGCCATACACACCAGAGAAAAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAACTCTCTTTCAGATCCATCCCCAGCGCCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCAAACCAAAAATCTCCCCACGTTTAACGGCa                                                                                                                                     >  1:95634/1‑250 (MQ=255)                                                                                                       gTAAGCCATACACACCAGAGAAAAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAACTCTCTTTCAGATCCATCCCCAGCGCCAGCGCCTGGCCGGGAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCAAACCAAAAATCTCCCCACGTTTAACGGCaa                                                                                                                                    >  1:260101/1‑249 (MQ=255)                                                                                                                 cacacCAGAGAAAAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAACTCTCTTTCAGATCCATCCCCAGCGCCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCAAACCAAAAATCTCCCCACGTTTAACGGCAAAGTTGACGTGAt                                                                                                                        >  2:37508/1‑251 (MQ=255)                                                                                                                   cacCAGAGAAAAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAACTCTCTTTCAGATCCATCCCCAGCGCCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCAAACCAAAAATCTCCCCACGTTTAACGGCAAAGTTGACGTGATCg                                                                                                                      <  1:307524/251‑1 (MQ=255)                                                                                                                                                         ttACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAACTCTCTTTCAGATCCATCCCCAGCGCCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCAAACCAAAAATCTCCCCACGTTTAACGGCAAAGTTGACGTGATCGGTGGCGGCAAAATCCCCAAATTTCTTGGTCAGTTCttt                                                                                >  1:5985/1‑251 (MQ=255)                                                                                                                                                           aCCGTCGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAACTCTCTTTCAGATCCATCCCCAGCGCCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCAAACCAAAACTCTCCCCACGTTTAACGGCAACGTTGACGTGATCGGTGGCGGCAAAATCCCCAAATTTCTTGGTCAGTTCTTTCg                                                                              >  2:150005/1‑251 (MQ=255)                                                                                                                                                                               gcgcCATATAGCCGAGATGCTGGCGCGCTTTACCGGAACTCTCTTTCAGATCCATCCCCAGCGCCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCAAACCAAAAATCTCCCCACGTTTAACGGCAAAGTTGACGTGATCGGTGGCGGCAAAATCCCCAAATTTCTTGGTCAGTTCTTTCGCTTCGATCTCCTTCTCGcc                                                           <  1:150005/250‑1 (MQ=255)                                                                                                                                                                                cgcCATATAGCCGAGATGCTGGCGCGCTTTACCGGAACTCTCTTTCAGATCCATCCCCAGCGCCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCAAACCAAAAATCTCCCCACGTTTAACGGCAAAGTTGACGTGATCGGTGGCGGCAAAATCCCCAAATTTCTTGGTCAGTTCTTTCGCTTCGATCACCGTCTCGCCgg                                                         >  1:212918/1‑251 (MQ=255)                                                                                                                                                                                 gcCATATAGCCGAGATGCTGGCGCGCTTTACCGGAACTCTCTTTCAGATCCATCCCCAGCGCCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCAAACCAAAAATCTCCCCACGTTTAACGGCAAAGTTGACGTGATCGGTGGCGGCAAAATCCCCAAATTTCTTGGTCAGTTCTTTCGCTTCGATCACCGTCTCGCCggg                                                        <  1:277736/251‑1 (MQ=255)                                                                                                                                                                                      ataGCCGAGATGCTGGCGCGATTTACCGGAACTATCTTTCAGATCCATCCCCAGCGCCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCAAACCAAAAATCTCCCCACGTTTAACGGCAAAGTTGACGTGATCGGTGGCGGCAAAATCCCCAAATTTCTTGGTCAGTTCTTTCGCTTCGATCACCGTCTCGCCGGGTGTGc                                                   >  2:363321/1‑251 (MQ=255)                                                                                                                                                                                        aGCCGCGATGCTGGAGCGCTTTAACGGAACTCTCTTTCATCACCATCCCCCGCGCCAGCGCCTGGCCGGCAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCAAACCACAAATCTCCCCACGCTTAACGGCAAAGTTGACGTGATCGGTGGCGGCAAAATCCCCAAATTTCTTGGTTAGTTCTTTCGCTTCGTTCACCACCTCGCCGGGTGTGccc                                                 >  2:155994/1‑250 (MQ=255)                                                                                                                                                                                        aGCCGAGATGCTGGCGCGCTTTACCGGAACTCTCTTTCAGATCCGTCCCCAGCGCCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGTTCGATTTACCCGCCCCGTTTGGCCCCAGCAAACCAAAAATCTCCCCACGTTTAACGGCAAAGTTGACGTTATCGGTGGCGGCAAAATCCCCAAATTTCTTGGTCAGTTCTTTCGCTTCGATCACCGTCTCGCCGGGTGTGcc                                                  <  2:188546/250‑1 (MQ=255)                                                                                                                                                                                          ccGAGATGCTGGCGCGCTTTACCGGAACTCTCTTTCAGATCCATCCCCAGCGCCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCAAACCAAAAATCTCCCCACGTTTAACGGCAAAGTTGACGTGATCGGTGGCGGCAAAATCCCCAAATTTCTTGGTCAGTTCTTTCGCTTCGATCACCGTCTCGCCGGGTGTGCCTTc                                               <  1:8217/251‑1 (MQ=255)                                                                                                                                                                                            gagaTGCTGGCGCGCTTTACCGGAACTCTCTTTCAGATCCATCCCCAGCGCCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCAAACCAAAAATCTCCCCACGTTTAACGGCAAAGTTGACGTGATCGGTGGCGGCAAAATCCCCAAATTTCTTGGTCAGTTCTTTCGCTTCGATCACCGTCTCGCCGGGTGTGCCTTCt                                              >  2:283890/1‑250 (MQ=255)                                                                                                                                                                                                   tGGCGCGCTTTACCGGAACTCTCTTTCAGATCCATCCCCAGCGCCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCAAACCAAAAATCTCCCCACGTTTAACGGCAAAGTTGACGTGATCGGTGGCGGCAAAATCCCCAAATTTCTTGGTCAGTTCTTTCGCTTCGATCACCGTCTCGCCGGGTGTGCCTTCTACCGTATg                                      >  2:130764/1‑251 (MQ=255)                                                                                                                                                                                                       gcgcTTTACCGGAACTCTCTTTAAGATCCATCCCCAGCGCCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCAAACCAAAAATCTCCCCACGTTTAACGGCAAAGATGACGTGATCGGTGGCGGCAAAATCCCCAAATTTCTTGGTCAGTTCTTTCGCTTCGATCACCGTCTCGCCCGGTGTGCATTCTACCGTATGtcat                                  >  2:354413/1‑248 (MQ=255)                                                                                                                                                                                                        cgcTTTACCGGAACTCTCTTTCAGATCCATCCCCATCGCCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTGACCCGCGCCg                                                                                                                                                                               <  2:60039/109‑1 (MQ=255)                                                                                                                                                                                                        cgcTTTACCGGAACTCTCTTTCAGATCCATCCCCAGCGACAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGGGGTCGATTTACCCGCGCCg                                                                                                                                                                               >  1:60039/1‑109 (MQ=255)                                                                                                                                                                                                          cTTTACCGGAACTCTCTTTCAGATCCATCCCCAGCGCCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCAAACCAAAAATCTCCCCACGTTTAACGGCAAAGTTGACGTGATCGGTGGCGGCAAAATCCCCAAATTTCTTGGTCAGTTCTTTCGCTTCGATCACCGTCTCGCCGGGTGTGCCTTCTACCGTATGTAATAtt                               >  1:175350/1‑251 (MQ=255)                                                                                                                                                                                                             tACCGGAACTCTCTTTCAGATCCATCCCCAGCGCCAGCGCCTGGCCGGGAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCAAACCAAAAGTCTCCCCACGTTTAACGGCAAAGTTGACGTGATCGGTGGCGGCAAAATCCCCAAATTTCTTGGTCAGTTCTTTCGCTTCGATCACCGTCTCGCCGGGTGTGCCTTCTACCGTATGTAATATTgc                             <  2:260101/250‑1 (MQ=255)                                                                                                                                                                                                              aCCGGAACTCTCTTTCAGATCCATCCCCAGCGCCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCAAACCAAAAATCTCCCCACGTTTAAc                                                                                                                                         <  2:214148/141‑1 (MQ=255)                                                                                                                                                                                                              aCCGGAACTCTCTTTCAGATCCATCCCCAGCGCCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCAAACCAAAAATCTCCCCACGTTTAAc                                                                                                                                         >  1:214148/1‑141 (MQ=255)                                                                                                                                                                                                                                       cccAGCGCCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCAAACCAAAAATCTCCCCACGTTTAACGGCAAAGTTGACGTGATCGGTGGCGGCAAAATCCCCAAATTTCTTGGTCAGTTCTTTCGCTTCGATCACCGTCTCGCCGGGTGTGCCTTCTACCGTATGTAATATTGCGCCCAGCGGCGATTCCGAGGTTCCGGc  >  2:15504/1‑251 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                     GTTTAAAACCTAATGGCAGTTCATCGGTGGCGTGGGAGGCGATACTTTTCAGGCCGAACGCCTCGCTCATGCGGGAGATTTTTTCGTTCTGCGCCCGACCGCGTAAGCCATACACACCAGAGAAAAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAACTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCAAACCAAAAATCTCCCCACGTTTAACGGCAAAGTTGACGTGATCGGTGGCGGCAAAATCCCCAAATTTCTTGGTCAGTTCTTTCGCTTCGATCACCGTCTCGCCGGGTGTGCCTTCTACCGTATGTAATATTGCGCCCAGCGGCGATTCCGAGGTTCCGGC  >  NC_000913/827583‑828063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑