Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 612,901 T→C G55G (GGT→GGC)  fes → ferric enterobactin esterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913612,9010TC95.5% 66.3 / ‑6.1 22G55G (GGT→GGC) fesferric enterobactin esterase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (12/9);  total (12/10)
Fisher's exact test for biased strand distribution p-value = 4.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGC‑AGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCAC‑TTTCTGGTGGCG‑TGATCCCCAAGGTTCTGAAGAATACTCGACGATAAAGCGCGTATGGGTCTACATCACTGGTGTGACCGATCACCATCAGAACAGCCAGCCCCAGTCGATGCAGCGAATTGCAGGCACTAACGTCTGGCAGTGGACGACACAACTCAATGCCAACTGGCGCGGCAGCTACTGCTTTATTCCCACCGAACGCGATGACATTTTTTCTGTACCATCCCCCGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCTGAACCTACAAAGCTGGAAAGGCG  >  NC_000913/612655‑613142
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                 
caaatgcaaatAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCAAAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGC‑AGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCAC‑TTTCTGGTGGCG‑TGATCCCCAAGGTTCTGAAGAATACTCGACGATAAAGCGCGTATGGGTCTACATCACTGGcgtgc                                                                                                                                                                                                                                               >  2:176101/1‑250 (MQ=255)
  aatgcaaatAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGC‑AGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCAC‑TTTCTGGTGGCG‑TGATCCCCAAGGTTCTGAAGAATACTCGACGATAAAGCGCGTATGGGTCTACATCACTGGCGTGAcc                                                                                                                                                                                                                                             >  1:239337/1‑251 (MQ=255)
                        attatCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGACG‑GCAAGGTGGTGGCCGGCCGAAA‑ATTGCCCGGAATGGGCGCGTCTTGATGGCGGAATTGTTGAGGTCACCTTTC‑GGTGGCGGTG‑TCCCCAAGGTTCTGAAGAATACTCGACGGTAAAGGGGGTAAGGGTTTACATCACTTGCCGGAACGATCACCATTCGAACaaccagcc                                                                                                                                                                                                                      >  1:333158/1‑251 (MQ=255)
                                  tatTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGC‑AGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGTAATGTTTGAGGTCAC‑TTTCTGGTGGCG‑TGATCCCCAAGGTTCTGAAGAATACTCGACGATAAAGCGCGTATGGGTCTACATCACTGGCGTGACCGATCACCATCAGAACAGCCAGCCCCAGTCGAt                                                                                                                                                                                                             >  2:255616/1‑251 (MQ=255)
                                                    aaaaTTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGC‑AGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCAC‑TTTCTGGTGGCG‑GGATCCCCAAGGTTCTGAAGAATACTCGACGATAAAGCGCGTATTGGTCGACATCACTGGCGTTACCGATCACCATCAGAACAGCCAGCCCCAGTCGATGCAGCGAATTGCAGGCAc                                                                                                                                                                                           <  1:29399/251‑1 (MQ=255)
                                                     aaaTTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGC‑AGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCAC‑TTTCTGGTGGCG‑TGATCCCCAAGGTTCTGAAGAATACTCGACGATAAAGCGCGTATGGGTCTACATCACTGGCGTGACCGATCACCATCAGAACAGCCAGCCCCAGTCGATGCAGCGAATTGCAGGCACt                                                                                                                                                                                          >  1:241802/1‑251 (MQ=255)
                                                                 aaaCATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGC‑AGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCAC‑TTTCTGGTGGCG‑TGATCCCCAAGGTTCTGAAGAATACTCGACGATAAAGCGCGTATGGGTCTACATCACTGGCGTGACCGATCACCATCAGAACAGCCAGCCCCAGTCGATGCAGCGAATTGCAGGCACTAACGTCTGGCa                                                                                                                                                                               <  1:205605/250‑1 (MQ=255)
                                                                             aTGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGC‑AGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCAC‑TTTCTGGTGGCG‑TGATCCCCAAGGTTCTGAAGAATACTCGACGATAAAGCGCGTATGGGTCTACATCACTGGCGTGACCGTTCACCATCAGAACAGCCAGCCCCAGTCGATGCAGCGAATTGCAGGCACTAACGTCTGGCAGTGGACGAcaca                                                                                                                                                                   >  1:290464/1‑250 (MQ=255)
                                                                              tGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGC‑AGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCAC‑TTTCTGGTGGCG‑TGATCCCCAAGGTTCTGAAGAATACTCGACGATAAAGCGCGTATGGGTCTACATCACTGGCGTGACCGATCACCATCAGAACAGCCAGCCCCAGTCGATGCAGCGAATTGCAGGCACTAACGTCTGGCAGTGGACGACACAAc                                                                                                                                                                 >  2:164364/1‑251 (MQ=255)
                                                                                                 gTAGGAAGTGAGAGCTGGTGGC‑AGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCAC‑TTTCTGGTGGCG‑TGATCCCCAAGGTTCTGAAGAATACTCGACGATAAAGCGCGTATGGGTCTACATCACTGGCGTGACCGATCACCATCAGAACAGCCAGCCCCAGTCGATGCAGCGAATTGCAGGCACTAACGTCTGGCAGTGGACGACACAACTCAATGCCAACTGGCGCgg                                                                                                                                              >  1:353182/1‑251 (MQ=255)
                                                                                                                         gTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCAC‑TTTCTGGTGGCG‑TGATCCCCAAGGTTCTGAAGAATACTCGACGATAAAGCGCGTATGGGTCTACATCACTGGCGTGACCGATCACCATCAGAACAGCCAGCCCCAGTCGATGCAGCGAATTGCAGGCACTACAGTCTGGCAGTGGCCGACCCAACTCAATGCCAACTGGCGCGGCAGCTACTGCTTTATTCCCACCg                                                                                                                       >  2:206576/1‑251 (MQ=255)
                                                                                                                                         gAATGGCAGCGGCTGAATGACGAAATGTTTGAGGTCAC‑TTTCTGGTGGCG‑TGATCCCCAAGGTTCTGAAGAATACTCGACGATAAAGCGCGTATGGGTCTACATCACTGGCGTGACCGATCACCATCAGAACAGCCAGCCCCAGTCGATGCAGCGAATTGCAGGCACTAACGTCTGGCAGTGGACGACACAACTCAATGCCAACTGGCGCGGCAGCTACTGCTTTATTCCCACCGAACGCGATGACAtttt                                                                                                       <  2:284429/251‑1 (MQ=255)
                                                                                                                                                                  tGTTTGAGGTCAC‑TTTCTGGTGGCG‑TGATCCCCAAGGTTCTGAAGAATACTCGACGATAAAGCGCGTATGGGTCTACATCACTGGCGTGACCGATCACCATCAGAACAGCCAGCCCCAGTCGATGCAGCGAATTGCAGGCACTAACGTCTGGCAGTGGACGACACAACTCAATGCCAACTGGCGCGGCAGCTACTGCTTTATTCCCACCGAACGCGATGACATTTTTTCTGTACCATCCCCCGATCGCCTc                                                                              >  1:36201/1‑251 (MQ=255)
                                                                                                                                                                     ttGAGGTCAC‑TTTCTGGTGGCG‑TGATCCCCAAGGTTCTGAAGAATACTCGACGATAAAGCGCGTATGGGTCTACATCACTGGCGTGACCGATCACCATCAGAACAGCCAGCCCCAGTCGATGCAGCGAATTGCAGGCACTAACGTCTGGCAGTGGACGACACAACTCAATGCCAACTGGCGCGGCAGCTACTGCTTTATTCCCACCGAACGCGATGACATTTTTTCTGTACCATCCCCCGATCGCCTCGa                                                                            >  1:167119/1‑250 (MQ=255)
                                                                                                                                                                                     ggtggCG‑TGATCCCCAAGGTTCTGAAGAATACTCGACGATAAAGCGCGTATGGGTCTACATCACTGGCGTGACCGATCACCATCAGAACAGCCAGCCCCAGTCGATGCAGCGAATTGCAGGCACTAACGTCTGGCAGTGGACGACACAACTCAATGCCAACTGGCGCGGCAGCTACTGCTTTATTCCCACCGAACGCGATGACATTTTTTCTGTACCATCCCCCGATCGCCTCGAATTGCGCGAAGGCt                                                              <  2:56873/249‑1 (MQ=255)
                                                                                                                                                                                        ggCG‑TGATCCCCAAGGTTCTGAAGAATACTCGACGATAAAGCGCGTATGGGTCTACATCACTGGCGTGACCGATCACCATCAGAACAGCCAGCCCCAGTCGATGCAGCGAATTGCAGGCACTAACGTCTGGCAGTGGACGACACAACTCAATGCCAACTGGCGCGGCAGCTACTGCTTTATTCCCACCGAACGCGATGACATTTTTTCTGTACCATCCCCCGATCGCCTCGAATTGCGCGAAGGCTGGCg                                                          <  1:288702/250‑1 (MQ=255)
                                                                                                                                                                                                 ccccAAGGTTCTGAAGAATACTCGACGATAAAGCGCGTATGGGTCTACATCACTGGCGTGACCGATCACCATCAGAACAGCCAGCCCCAGTCGATGCAGCGAATTGCAGGCACTAACGTCTGGCAGTGGACGACACAACTCAATGCCAACTGGCGCGGCAGCTACTGCTTTATTCCCACCGAACGCGATGACATTTTTTCTGTACCATCCCCCGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTAtt                                                 <  1:196108/251‑1 (MQ=255)
                                                                                                                                                                                                  cccAAGGTTCTGAAGAATACTCGACGATAAAGCGCGTATGGGTCTACATCACTGGCGTGACCGATCACCATCAGAACAGCCAGCCCCAGTCGATGCAGCGAATTGCAGGCACTAACGTCTGGCAGTGGACGACACAACTCAATGCCAACTGGCGCGGCAGCTACTGCTTTATTCCCACCGAACGCGATGACATTTTTTCTGTACCATCCCCCGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTa                                                <  1:164364/251‑1 (MQ=255)
                                                                                                                                                                                                        gTTCTGAAGAATACTCGACGATAAAGCGCGTATGGGTCTACATCACTGGCGTGACCGATCACCATCAGAACAGCCAGCCCCAGTCGATGCAGCGAATTGCAGGCACTAACGTCTGGCAGTGGACGACACAACTCAATGCCAACTGGCGCGGCAGCTACTGCTTTATTCCCACCGAACGCGATGACATTTTTTCTGTACCATCCCCCGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTAcccc                                            >  1:184850/1‑249 (MQ=255)
                                                                                                                                                                                                                                   gcgTATGGGTCTACATCACTGGCGTGACCGATCACCATCAGAACAGCCAGCCCCAGTCGATGCAGCGAATTGCAGGCACTAACGTCTGGCAGTGGACGACACAACTCAATGCCAACTGGCGCGGCAGCTACTGCTTTATTCCCACCGAACGCGATGACATTTTTTCTGTACCATCCCCCGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCTGAACCTACa                >  1:349349/1‑250 (MQ=255)
                                                                                                                                                                                                                                    cgTATGGGTCTACATCACTGGCGTGACCGATCACCATCAGAACAGCCAGCCCCAGTCGATGCAGCGAATTGCAGGCACTAACGTCTGGCAGTGGACGACACAACTCAATGCCAACTGGCGCGGCAGCTACTGCTTTATTCCCACCGAACGCGATGACATTTTTTCTGTACCATCCCCCGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCTGAACCTACaaa              <  2:302457/251‑1 (MQ=255)
                                                                                                                                                                                                                                               acagcattGGTGTGACCAATCACCATCAGAACAGCCAGTGCCAGTCGATGCAGCGAATTGCAGGCACTAACGTCGGGAAGTGGACGACACAATTGAATGCCAACTTGGGCGGCAGCTACAGCTGTATTTTCTCCGAACTGGATGACTGTTGGTCTGTATCATCCCCCAAACGCCTCGAGTTGCGCGAAGGCGGGCGAACCCTATTTCCCCAGGCGATAGCCGATCCGCTGAACCTACAAAGCTGGaaa      <  1:346752/241‑1 (MQ=255)
                                                                                                                                                                                                                                                cATCACTGGCGTGACCGATCACCATCAGAACAGCCAGCCCCAGTCGATGCAGCGAATTGCAGGCACTAACGTCTGGCAGTGGACGACACAACTCAATGCCAACTGGCGCGGCAGCTACTGCTTTATTCCCACCGAACGCGATGACATTTTTTCTGTACCATCCCCCGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCTGAACCTACAAAGCTGGAAAggcg  <  1:343414/251‑1 (MQ=255)
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                 
CAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGC‑AGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCAC‑TTTCTGGTGGCG‑TGATCCCCAAGGTTCTGAAGAATACTCGACGATAAAGCGCGTATGGGTCTACATCACTGGTGTGACCGATCACCATCAGAACAGCCAGCCCCAGTCGATGCAGCGAATTGCAGGCACTAACGTCTGGCAGTGGACGACACAACTCAATGCCAACTGGCGCGGCAGCTACTGCTTTATTCCCACCGAACGCGATGACATTTTTTCTGTACCATCCCCCGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGATCCGCTGAACCTACAAAGCTGGAAAGGCG  >  NC_000913/612655‑613142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑