Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	462,395	A→G	T161A (ACT→GCT)	ppiD →	periplasmic folding chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	462,395	0	A	G	100.0%	125.3 / NA	37	T161A (ACT→GCT)	ppiD	periplasmic folding chaperone
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/21);  total (16/21)

AAGGCTATATGAAAACCCTGCGTCAACAGGTGCTGAATCGTCTGATCGACGAGGCGCTGCTGGATCAGTACGCACG‑TGAGCTGAAACTGGGTATCAGCGATGAGCAGGTTAAACAGGCGATTTTCGCGACCCCAGCCTTCCAGGTTGACGGCAAATTTGATAACAGCCGCTATAACGGTATCCTCAACCAGATGGGGATGACCGCCGATCAGTACGCCCAGGCGCTGCGTAACCAGCTCACTACCCAACAGCTGATTAACGGCGTTGCCGGTACCGATTTTATGCTGAAAGGTGAAACCGACGAGCTGGCGGCACTGGTCGCGCAACAACGCGTGGTGCGTGAGGCGACTATCGATGTTAACGCGCTGGCGGCGAAGCAGCCTGTGACCGAACAGGAGATTGCCAGCTACTACGAACAAAACAAAAACAATTTCATGACGCCGGAACAATTCCGCGTGAGTTACATCAAGCTGGAT  >  NC_000913/462156‑462631                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                             aaGGCTATATGAAAACCCTGCGTCAACAGGTGCTGAATCGTCTGATCGACGAGGCGCTGCTGGATCAGTACGCACG‑TGAGCTGAAACTGGGTATCAGCGATGAGCAGGTTAAACAGGCGATTTTCGCGACCCCAGCCTTCCAGGTTGACGGCAAATTTGATAACAGCCGCTATAACGGTATCCTCAACCAGATGGGGATGACCGCCGATCAGTACGCCCAGGCGCTGCGTAACCAGCTCGCTACCCAAc                                                                                                                                                                                                                                     >  1:75776/1‑249 (MQ=255)           gAAAACCCTGCGTCAACAGGTGCTGAATCGTCTGATCGACGAGGCGCTGCTGGATCAGTACGCACG‑TGAGCTGAAACTGGGTATCAGCGATGAGCAGGTTAAACAGGCGATTTTCGCGACCCCAGCCTTCCAGGTTGACGGCAAATTTGATAACAGCCGCTATAACGGTATCCTCAACCAGATGGGGATGACCGCCGATCAGTCCGCCCAGGCGCTGCGTAACCAGCTCGCTACCCAACCGCTGATTAACg                                                                                                                                                                                                                         >  2:191307/1‑251 (MQ=255)                   ggcgtcatagggtccTGAATGGTCTGACCGGCGGGGCGCGGCTGGAGCAGTACGCACG‑TGAGCTGAAGCTGGGTATAGGAGAGGAGCAGGTTAAACAGGCTATTTTCGCACCCCCAGCCTTCCAGGTGAACGGAAAATTTGATAACAGCCGCTATAACGGTATCCTCAACCAGATGGGGATGACCGCCGATCAGTACGCCCAGGCGCTGCGTAACCAGCTCGCTACCCAACAGCTGATTAACGGCGTTGcc                                                                                                                                                                                                                 <  2:77136/237‑1 (MQ=255)                      gtaaaCAGGTGCTGAAGCGTCGGATCGACGAGGCGCTGCTGGATCAGTACGCACG‑TGAGCTGAAACTGGGTATCAGCGATGAGCAGGTTAAACAGGCGATTTTCGGGACCCCAGCCTTCCAGGTTGACGGCAAATTTGATAACAGCCGCTATAACGGTATCCTCAACCAGATGGGGATGACCGCCGATCAGTACGCCCAGGCGCTGCGTAACCAGCTCGCTACCCAACAGCTGATTAACGGCGTTGCCGGt                                                                                                                                                                                                              <  1:337081/248‑1 (MQ=255)                       tCAACAGGTGCTGAATCGTCTGATCGACGAGGCGCTGCTGGATCAGTACGCACG‑TGAGCTGAAACTGGGTATCAGCGATGAGCAGGTTAAACAGGCGATTTTCGCGACCCCAGCCTTCCAGGTTGACGGCAAATTTGATAACAGCCGCTATAACGGTATCCTCAACCAGATGGGGATGACCGCCGATCAGTACGCCCAGGCGCTGCGTAACCAGCTCGCTACCCAACAGCTGATTAACGGCGTTGCCGGt                                                                                                                                                                                                              >  1:151994/1‑250 (MQ=255)                         aaCAGGTGCTGAATGGTCGGACCGAGGAGGCGCTGGGGGATCAGTACGCACG‑TGAGCTGAAACAGGGTATCAGCGAGGAGCAGGTTAAACGGGCGATTTTCGCGACCCCAGCCTTCCAGGTTAGCGGCAAATTGGATACCAGCCGCTATAACGGTATCCTCAACAAGATGGGGATGACCGCCGATCAGTACGCCCAGGCGCTGCGTAACCAGCTCGCTACCCAACAGCTGATTAACGGCGTTGCCGGTAcc                                                                                                                                                                                                           <  2:205767/251‑1 (MQ=255)                           cAGGTGCTGAATCGTCTGATCGACGAGGCGCTGCTGGATCAGTACGCACG‑TGAGCTGAAACTGGGTATCAGCGATGAGCAGGTTAAACAGGCGATTTTCGCGACCCCAGCCTTCCAGGTTGACGGCAAATTTGATAACAGCCGCTATAACGGTATCTTCAACCAGATGGGGATGACCGCCGATCAGTACGCCCAGGCGCTGCGTAACCAGCTCGCTACCCAACAGCTGATTAACGGCGTTGCCGGTACCg                                                                                                                                                                                                          >  1:188046/1‑250 (MQ=255)                            gggTGCTGAATCGTCTGTTCGACGAGGCGCTGCTGGATCAGTACGCACG‑TGAGCTAAAACTGGGTTTCAGCGTTGAGCAGGTTAAACAGGCGATTTTCGCGCCCCCAGCCTTCCAGGTTGACGGCAAATTTGATAACAGCCGCTATAACGGTATCCTCAACCAGATGGGGATGACCGCCGATCAGTACGCCCAGGCGCTGCGTAACCAGCTCGCTACCCAACAGCTGATTAACGGCGTTGCCGGTACCGAt                                                                                                                                                                                                        <  1:17099/250‑1 (MQ=255)                                         tCTGATCGACGAGGCGCGGCGGGATCAGTACCCCCG‑TTGGCTGAAACGGGGTATCAGCGTGGAGCAGGTTAAACAGGGTTTTTTCGCGACCCAAGCCTTCCAGGTTGACGGCAAATTTGATACCAGCCGTTATAACGGTATCCTCAACCAGATGGGGATGACCGCCGATCAGTACGCCCAGGCGCTGCGTAACCAGCTCGCTACCCAGCAGCTGATTAACGGCGTTGCCGGTACCGATTTTATGCTGa                                                                                                                                                                                              <  2:354058/248‑1 (MQ=255)                                                gacgaGGCGCGGCGGGATCAGTA‑GGACGGTGAGCGAAAACTGGGTACCAGCGATGAGCAGGTTAAAAGGGGGATTTTGGCGCCCCCAGCCTCCCAGGTGGACGAAAATTTTAATAACAGCCGATATAACGGTATCCTCAACCAGATGGGGATGACCGCCGATCAGTACGCCCAGGCGCTGCGTAACCAGCTCGCTACCCAACAGCTGATTAACGGCGTTGCCGGTACCGATTTTATGCTGAAAGGTGAAAc                                                                                                                                                                                    <  2:293754/251‑1 (MQ=255)                                                      gcgcGGCTGGATAATTACGCCCT‑TGACCTGAAACTGGGTATCAGCGTTGAGCAGGTTAAACAGGCGATTTTCTCGACCCTAGCCTTCCAGGTTGACGGCAAATTTGAAAACAGCCGCTATAACGGTAGCCTCAACCAGATGGTGAGCACCGCCGATCAGTACGCCCAGGCGCTGCGTAACCAGCTCGCTACCCAACAGCTGATTAGCGGCGTTGCCGGTACCGATTTTATGCTGAAAGGTGAAACcgacga                                                                                                                                                                              <  1:16277/251‑1 (MQ=255)                                                           gctgGACCAGTACGCACG‑TGAGCGAAAACTGGGTATCAGCGATGAGCAGGTTAAACAGGCGATTTTCGCGCCCCCAGCCTTCCAGGTTGACGGCAAATTTGTTAACAGCCGCTATAACGTTATCCTCAACCAGATGGGGATGACCGCCGATCAGTACGCCCAGGCGCTGCGTAACCAGCTCGCTACCCAACAGCTGATTAACGGCGTTGCCGGTACCGATTTTATGCTGAAAGGTGAAACCGACGAGCTg                                                                                                                                                                          <  1:51941/250‑1 (MQ=255)                                                                   aGTACGCACG‑TGAGCTGAAACTGGGTATCAGCGATGAGCAGGTTAAACAGGCGATTTTCGCGACCCCAGCCTTCCAGGTTGACGGCAAATTTGATAACAGCCGCTATAACGGTATCCTCAACCAGATGGGGATGACCGCCGATCAGTACGCCCAGGCGCTGCGTAACCAGCTCGCTACCCAACAGCTGATTAACGGCGTTGCCGGTACCGATTTTATGCTGAAAGGTGAAACCGACGAGCTGGCGGCACTg                                                                                                                                                                 >  1:13232/1‑251 (MQ=255)                                                                      aCGCACG‑TAAGCTGAAACTGGGTTTCGCCGATTAGCAGTTAAAACAGGCGATTTTCGCGACCCCCGCCTTCCAGGTTGACGGCAAATTTGATAACAGCCGCTATAACGGTATCCTCAACCAGATGGGGATGACCGCCGATCAGTACGCCCAGGCGCTGCGTAACCAGCTCGCTACCCAACAGCTGATTAACGGCGTTGCCGGTACCTATTTTATGCTGAAAGGTGAAACCGACGAGCTGGCTGCTCTGGTc                                                                                                                                                              <  1:114278/251‑1 (MQ=255)                                                                                     aaaCTGGGTATCAGCGATGAGCAGGTTAAACAGGCGATTTTCGCGACCCCAGCCTTCCAGGTTGACGGCAAATTTGATAACAGCCGCTATAACGGTATCCTCAACCAGATGGGGATGACCGCCGATCAGTACGCCCAGGCGCTGCGTAACCAGCTCGCTACCCAACAGCTGATTAACGGCGTTGCCGGTACCGATTTTATGCTGAAAGGTGAAACCGACGAGCTGGCGGCACTGGTCGCGCAACAAcgcgg                                                                                                                                                >  2:64403/1‑250 (MQ=255)                                                                                         tGGGTATCAGCGATGAGCAGGTTAAACAGGCGATTTTCGCGACCCCAGCCTTCCAGGTTGACGGCAAATTTGATAACAGCCGCTATAACGGTATCCTCAACCAGATGGGGATGACCGCCGATCAGTACGCCCAGGCGCTGCGTAACCAGCTCGCTACCCAACAGCTGATTAACGGCGTTGCCGGTACCGATTTTATGCTGAAAGGTGAAACCGACGAGCTGGCGGCACTGGTCGCGCAACAACGCgtggtg                                                                                                                                            >  1:311460/1‑251 (MQ=255)                                                                                                cAGCGATGAGGAGGTAAATCAGGCGATTTTCGCCCCCCCAGCCTTCCAGTTTGCCGTCAATTTTTATACCAGCCGCTATAACGGTATCCTCAACCAGATGGGGATGACCGCCGATCAGTACGCCCAGGCGCTGCGTAACCAGCTCGCTACCCAACAGCTGATTAACGGCGTTGCCGGTACCGATTTTATGCTGAAAGGTGAAACCGACGAGCTGGCGGCACTGGTCGCGCAACAACGCGTGGTGCGTGAgg                                                                                                                                     <  2:114551/251‑1 (MQ=255)                                                                                                        aGCAGTTTAAACAGGCGATTTTCGCGACCCCAGCCTTCCAGGTTGACGGCAAATTTGATACCAGCCGCTATAACGGTAGCCTCAACCAGATGGGGATGACCGCCGATCAGTACGCCCAGGCGCTGCGTAACCAGCTCGCTACCCAACAGCTGATTAACGGCGTTGCCGGTACCGATTTTATGCTGAAAGGTGAAACCGACGAGCTGGCGGCACTGGTCGCGCAACAACGCGTGGTGCGTGAGGCGACTATc                                                                                                                             <  1:74670/251‑1 (MQ=255)                                                                                                           aGGTTAAACAGGCGATTTTCGCGACCCCAGCCTTCCAGGTTGACGGCAAATTTGATAACAGCCGCTATAACGGTATCCTCAACCAGATGGGGATGACCGCCGATCAGTACGCCCAGGCGCTGCGTAACCAGCTCGCTACCCAACAGCTGATTAACGGCGTTGCCGGTACCGATTTTATGCTGAAAGGTGAAACCGACGAGCTGGCGGCACTGGTCGCGCAACAACGCGTGGTGCGTGAGGCGACTATCGAt                                                                                                                          >  1:121357/1‑251 (MQ=255)                                                                                                                aaaCAGGCGATTTTCGCGACCCCAGCCTTCCAGTTTGACGGCAAATTTGATAACAGCCGCTATACCGGTATCCTCAACCAGATGGGGATGACCGCCGATCAGTACGCCCAGGCGCTGCGTAACCAGCTCGCTACCCAACAGCTGATTAACGGCGTTGCCGGTACCGCTTTTATTCTGAAAGGTGAAACTGACGAGCTGGCCGCACTGGTCCCGCAACAACGCGTGGTGCGTGCGGCGTCTATCGATGTTa                                                                                                                      >  2:5371/1‑250 (MQ=255)                                                                                                                     ggCGATTTTCGCGACCCCAGCCTTCCAGGTTGACGGCAAATTTGATAACAGCCGCTATAACGGTATCCTCAACCAGATGGGGATGACCGCCGATCAGTACGCCCAGGCGCTGCGTAACCAGCTCGCTACCCAACAGCTGATTAACGGCGTTGCCGGTACCGATTTTATGCTGAAAGGTGAAACCGACGAGCTGGCGGCACTGGTCGCGCAACAACGCGTGGTGCGTGAGGCGACTATCGATGTTAACgcgc                                                                                                                <  1:206492/251‑1 (MQ=255)                                                                                                                            ttCGCGACCCCAGCCTTCCAGGTTGACGGCAAATTTGATAACAGCCGCTATAACGGTATCCTCAACCAGATGGGGATGACCGCCGATCAGTACGCCCAGGCGCTGCGTAACCAGCTCGCTACCCAACAGCTGATTAACGGCGTTGCCGGTACCGATTTTATGCTGAAAGGTGAAACCGACGAGCTGGCGGCACTGGTCGCGCAACAACGCGTGGTGCGTGAGGCGACTATCGATGTTAACGCGCTggcggc                                                                                                         >  1:331283/1‑251 (MQ=255)                                                                                                                              cgcgACCCCAGCCTTCCAGGTGGACGGCAAATTTGATAACAGCCGCTATAACGGTATCCTCAACCAGATGGGGATGACCGCCGATCAGTACGCCCAGGCGCTGCGTAACCAGCTCGCTACCCAACAGCTGATTAACGGCGTTGCCGGTACCGATTTTATGCTGAAAGGTGAAACCGACGAGCTGGCGGCACTGGTCGCGCAACAACGCGTGGTGCGTGAGGCGACTATCGATGTTAACGCGCTGGCGGCGa                                                                                                       <  1:201406/251‑1 (MQ=255)                                                                                                                                         ccTTCCAGGTTGACGGCAAATTTGATAACAGCCGCTATAACGGTATCCTCAACCAGATGGGGATGACCGCCGATCAGTACGCCCAGGCGCTGCGTAACCAGCTCGCTACCCAACAGCTGATTAATGGCGTTGCCGGTACCGATTTTATGCTGAAAGGTGAAACCGACGAGCTggcg                                                                                                                                                                       >  2:251874/1‑176 (MQ=255)                                                                                                                                         ccTTCCAGGTTGACGGAAAATTTGATACCAGCCGCTATAACGGTATCCTCAACCAGATGGGGATGACCGCCGATCAGTACGCCCAGGCGCTGCGTAACCAGCTCGCTACCCAACAGCTGATTAACGGCGTTGCCGGTACCGATTTTATGCTGAAAGGTGAAACCGACGAGCTggcg                                                                                                                                                                       <  1:251874/176‑1 (MQ=255)                                                                                                                                          cTTCCAGGTTGACGGCAAATTTGATAACAGCCGCTATAACGGTATCCTCAACCAGATGGGGATGACCGCCGATCAGTACGCCCAGGCGCTGCGTAACCAGCTCGCTACCCAACAGCTGATTAACGGCGTTGCCGGTACCGATTTTATGCTGAAAGGTGAAACCGACGAGCTGGCGGCACTGGTCGCGCAACAACGCGTGGTGCGTGAGGCGACTATCGATGTTAACGCGCTGGCGGCGAAGCAGCCtgtg                                                                                            >  1:121956/1‑250 (MQ=255)                                                                                                                                                   ggcggaaaaattggaaaccAGCCGCTAAACCGGTCTCCTCAACCAGATGGGGATGACCGCCGATCAGTACGCCCAGGCGCTGCGTAACCAGCTCGCTACCCAACAGCTGATTAACGGCGTTGCCGGTACCGATTTTATGCTGAAAGGTGAAACCGACGAGCTGGCGGCACTGGTCGCGCAACAACGCGTGGTGCGTGAGGCGACTATCGATGTTAACGCGCTGGCGGCGAAGCAGCCTGTGACCGAACAgg                                                                                  <  2:131498/233‑1 (MQ=255)                                                                                                                                                    gacgcaaaaTTTAATAACACCCCCTATACCGGTATCCTCACCCAGATGGGGATGACCGCCGATCAGTACGCCCAGGCGCTGCGTAACCAGCTCGCTACCCAACAGCTGATTAACGGCGTTGCCGGTACCGATTTTATGCTGAAAGGTGAAACCGACGAGCTGGCGGCACTGGTCGCGCAACAACGCGTGGTGCGTGAGGCGACTATCGATGTTAACGCGCTGGCGGCGAAGCAGCCTGTGACCGAACAGga                                                                                 <  2:13232/245‑1 (MQ=255)                                                                                                                                                                         cGCTATAGCGGTATCCTCAACCAGATGGGGATGACCGCCGATCAGTACGCCCAGGCGCTGCGTAACCAGCTCGCTACCCAACAGCGGATTAACGGCGTTGCCGGTACCGATTTTATGCTGAAAGGTGAAACCGACGAGCTGGCGGCACTGGTCGCGCAACAACGCGTGGTGCGTGAGGCGACTATCGATGTTAACGCGCTGGCGGCGAAGCAGCCTGTGACCGAACAGGAGATTGCCAGCTACTACGaaca                                                            <  2:212694/251‑1 (MQ=255)                                                                                                                                                                         cGCTATAACGGTATCCTCAACCAGATGGGGATGACCGCCGATCAGTACGCCCAGGCGCTGCGTAACCAGCTCGCTACCCAACAGCTGATTAACGGCGTTGCCGGTACCGATTTTATGCTGAAAGGTGAAACCGACGAGCTGGCGGCACTGGTCGCGCAACAACGCGTGGTGCGTGAGGCGACTATCGATGTTAACGCGCTGGCGGCGAAGCAGCCTGTGACCGAACAGGAGATTGCCAGCTACTACGaac                                                             >  2:278053/1‑250 (MQ=255)                                                                                                                                                                                 gggTATCCACACCCAGATGGGGGTGCCCGCCGATCAGTACGCCCAGGCGCTGCGTAACCAGCTCGCTACCCAACAGCTGATTAACGGCGTTGCCGGTACCGATTTTATGCTGAAAGGTGAAACCGACGAGCTGGCGGCACTGGTCGCGCAACAACGCGTGGTGCGTGAGGCGACTATCGATGTTAACGCGCTGGCGGCGAAGCAGCCTGTGACCGAACAGGAGATTGCCAGCTACTACGAAcaaaacaaaa                                                    <  2:193034/250‑1 (MQ=255)                                                                                                                                                                                       ccTCAACCAGATGGGGATGACCGCCGATCAGTACGCCCAGGCGCTGCGTAACCAGCTCGCTACCCAACAGCTGATTAACGGCGTTGCCGGTACCGATTTTATGCTGAAAGGTGAAACCGACGAGCTGGCGGCACTGGTCGCGCAACAACGCGTGGTGCGTGAGGCGACTATCGATGTTAACGCGCTGGCGGCGAAGCAGCCTGTGACCGAACAGGAGATTGCCAGCTACTACGAACAAAACAAAAACAAtt                                              >  2:250401/1‑251 (MQ=255)                                                                                                                                                                                          cAACCAGAGGGGGATGACCGCCGATCAGTACGCCCAGGCGCTGCGTAACCAGCTCGCTACCCAACAGCTGATTAACGGCGTTGCCGGTACCGATTTTATGCTGAAAGGTGAAACCGACGAGCTGGCGGCACTGGTCGCGCAACAACGCGTGGTGCGTGAGGCGACTATCGATGTTAACGCGCTGGCGGCGAAGCAGCCTGTGACCGAACAGGAGATTGCCAGCTACTACGAACAAAACAAAAACAAttt                                             <  1:250401/249‑1 (MQ=255)                                                                                                                                                                                                         gACCGCCGATCAGTACGCCCAGGCGCTGCGTAACCAGCTCGCTACCCAACAGCTGATTAACGGCGTTGCCGGTACCGATTTTATGCTGAAAGGTGAAACCGACGAGCTGGCGGCACTGGTCGCGCAACAACGCGTGGTGCGTGAGGCGACTATCGATGTTAACGCGCTGGCGGCGAAGCAGCCTGTGACCGAACAGGAGATTGCCAGCTACTACGAACAAAACAAAAACAATTTCATGACGCCGGAACAAt                            >  2:283272/1‑251 (MQ=255)                                                                                                                                                                                                             gccgATCAGTACGCCCAGGCGCTGCGTAACCAGCTCGCTACCCAACAGCTGATTAACGGCGTTGCCGGTACCGATTTTATGCTGAAAGGTGAAACCGACGAGCTGGCGGCACTGGTCGCGCAACAACGCGTGGTGCGTGAGGCGACTATCGATGTTAACGCGCTGGCGGCGAAGCAGCCTGTGACCGAACAGGAGATTGCCAGCTACTACGAACAAAACAAAAACAATTTCATGACGCCGGAACAATTCcg                        <  2:159259/251‑1 (MQ=255)                                                                                                                                                                                                                   cAGTACGACCAGGAGCTGCGTAACCAGCTCGCTACCCAACCGCTGATTAACGGCGTTGCCGGTACCGATTTTATGCTGAAAGGTGAAACCGACGAGCTGGCGGCACTGGTCGCGCAACAACGCGTGGTGCGTGAGGCGACTATCGATGTTAACGCGCTGGCGGCGAAGCAGCCTGTGACCGAACAGGAGATTGCCAGCTACTACGAACAAAACAAAAACAATTTCATGACGCCGGAACAATTCCGCGTg                    >  2:211712/1‑249 (MQ=255)                                                                                                                                                                                                                                    gCGTAACCAGCTCGCTACCCAACAGCTGATTAACGGCGTTGCCGGTACCGATTTTATGCTGAAAGGTGAAACCGACGAGCTGGCGGCACTGGTCGCGCAACAACGCGTGGTGCGTGAGGCGACTATCGATGTTAACGCGCTGGCGGCGAAGCAGCCTGTGACCGAACAGGAGATTGCCAGCTACTACGAACAAAACAAAAACAATTTCATGACGCCGGAACAATTCCGCGTGAGTTACATCAAGCTGGAt  <  1:132103/250‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                             AAGGCTATATGAAAACCCTGCGTCAACAGGTGCTGAATCGTCTGATCGACGAGGCGCTGCTGGATCAGTACGCACG‑TGAGCTGAAACTGGGTATCAGCGATGAGCAGGTTAAACAGGCGATTTTCGCGACCCCAGCCTTCCAGGTTGACGGCAAATTTGATAACAGCCGCTATAACGGTATCCTCAACCAGATGGGGATGACCGCCGATCAGTACGCCCAGGCGCTGCGTAACCAGCTCACTACCCAACAGCTGATTAACGGCGTTGCCGGTACCGATTTTATGCTGAAAGGTGAAACCGACGAGCTGGCGGCACTGGTCGCGCAACAACGCGTGGTGCGTGAGGCGACTATCGATGTTAACGCGCTGGCGGCGAAGCAGCCTGTGACCGAACAGGAGATTGCCAGCTACTACGAACAAAACAAAAACAATTTCATGACGCCGGAACAATTCCGCGTGAGTTACATCAAGCTGGAT  >  NC_000913/462156‑462631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑