Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	443,588	G→A	A18A (GCC→GCT)	yajL ←	protein/nucleic acid deglycase 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	443,588	0	G	A	100.0%	116.2 / NA	34	A18A (GCC→GCT)	yajL	protein/nucleic acid deglycase 3
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/16);  total (18/16)

AACGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCACCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCACTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCGCGCGGGCGCGGCGTAAGA‑GAAAACCATTGATATAGTCGATTTCAGTGTGGCGCAGCGCGCGGATATCCTGCAACATCGACGAGATATTTTCCGCTGTGGCATCAATCACCTGCATCAC  >  NC_000913/443350‑443825                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                               aaCGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCACCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACAGCTTCAGTctct                                                                                                                                                                                                                                    <  1:81463/251‑1 (MQ=255)             gTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCACCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACAGCTTCAGTCTCTTCACTACCAgg                                                                                                                                                                                                                         <  1:75035/250‑1 (MQ=255)                       ttCAACCAGGAAAGTGCTATCGCCAAAACACTCCGAGCCTTTAATGCCACCACGCAGCACGATAACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGCGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCCGATCGATAGAGAGGACAGCTTCAGTTCCTTCACTACCAGGGGCGAGGCaa                                                                                                                                                                                                               >  2:374884/1‑250 (MQ=255)                           aCCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCACCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACAGCTTCAGTCTCTTCACTACCAGGGGCGAGGCAAAcccg                                                                                                                                                                                                          >  2:206840/1‑249 (MQ=255)                            ccAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCACCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACAGCTTCAGTCTCTTCACTACCAGGGGCGAGGCAAACCAGt                                                                                                                                                                                                         <  1:127144/251‑1 (MQ=255)                               gcagAGTGCCATCGCAAAAACACACCGCGCCGTTATTGCCCCCAGGCAGCACGACCACGTCATATTCGCCACCACCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTTGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACAGCTTCAGTCTCTTCACTACCAGGGGCGAGGCAAACCAGTGcc                                                                                                                                                                                                      <  2:288970/251‑1 (MQ=255)                                                          cgcCTTTAATGCCACCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACAGCTTCAGTCTCTTCACTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCctttct                                                                                                                                                                           <  1:79397/251‑1 (MQ=255)                                                             cTTTAATGCCACCAGGCAGCACGATCACGTCATCTTCGCCTTCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTAACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGCGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGAAAGCTTCAGTCTCTTCACTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCtttt                                                                                                                                                                        >  2:367939/1‑251 (MQ=255)                                                                 aaTGCCACCAGGCAGCACGATCACGTCATATTCGCCCTCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACAGCTTCAGTCTCTTCACTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTAcc                                                                                                                                                                    >  2:323049/1‑251 (MQ=255)                                                                  aTGCCACCAGGCAGCACGATCACGCCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACAAGCAGATCGATAGTGGTGACAGCTTCAGTCTCTTCACTACCAGGGGCGAGGCAAACCAGTGCAGATGCGCTCTTATTCACTCCCCTTTCTTTTTAcc                                                                                                                                                                    >  2:294567/1‑250 (MQ=255)                                                                       accaGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACAGCTTCAGTCTCTTCACTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTc                                                                                                                                                               >  1:135017/1‑250 (MQ=255)                                                                             cagcaCGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTCTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACAGCTTCAGTCTCTTCACTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAgg                                                                                                                                                        >  2:254125/1‑251 (MQ=255)                                                                                  cGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACAGCTTCAGTCTCTTCACTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCgggg                                                                                                                                                   >  1:234589/1‑250 (MQ=255)                                                                                    aTCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACAGCTTCAGTCTCTTCACTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGgtgt                                                                                                                                                 >  1:276257/1‑251 (MQ=255)                                                                                          tCATATTCGCCATCAGCCCCTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCTCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACAGCTTCAGTCTCTTCACGACCAGGGGCGAGGCAAACCATTGCCGATGCGCTCATATTCACTCTCcttt                                                                                                                                                                             <  2:358978/217‑1 (MQ=255)                                                                                          tCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGAGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGCTAGTGGTGACAGCTTCAGTCTCTTCACTACCAGGGGCGAGGCAAACAAGTGCCGATGCGCTCATATTCACTCTCcttt                                                                                                                                                                             >  1:358978/1‑217 (MQ=255)                                                                                                 cGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACAGCTTCAGTCTCTTCACTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACggg                                                                                                                                    >  2:3181/1‑250 (MQ=255)                                                                                                    cATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACAGCTTCAGTCTCTTCACTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGtttttccggacgggaa                                                                                                                                  >  2:241544/1‑238 (MQ=255)                                                                                                                       cggcgCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACAGCTTCAGTCTCTTCACTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTTTTTTCCGGTACGGCAATCCCATGCgcgcgggcgcgg                                                                                                              <  2:369272/251‑1 (MQ=255)                                                                                                                                          ttCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACAGCTTCAGTCTCTTCACTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGGGTTTTCCGGTACAGCAATCCCATGCGCGCGGGCCCGGCGGAagatcaacacatttg                                                                                           >  2:67213/1‑239 (MQ=255)                                                                                                                                           tCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACAGCTTCAGTCTCTTCACTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCGCGCGGGCGCGGCGTAAGA‑GAAAACCATTg                                                                                           <  1:196346/249‑1 (MQ=255)                                                                                                                                            cACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACAGCTTCAGTCTCTTCACTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACg                                                                                                                                      <  2:247714/206‑1 (MQ=255)                                                                                                                                            cACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACAGCTTCAGTCTCTTCACTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGGGTTTTCCGGTACg                                                                                                                                      >  1:247714/1‑206 (MQ=255)                                                                                                                                                 cgcgcgAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGCACCAGCAGATCGATAGTGGTGACAGCTTCAGTCTCTTCACTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGGACGGCAATCCCATGCGCGCGGGCGCGGCGTAAGA‑GAAAACCATTGATATAGTc                                                                                   >  1:166695/1‑251 (MQ=255)                                                                                                                                                         cAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACAGCTTCAGTCTCTTCACTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCGCGCGGGCGCGGCGTAAGA‑GAAAACCATTGATATAGTCGATTTCAg                                                                           <  1:284261/251‑1 (MQ=255)                                                                                                                                                            gTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACAGCTTCAGTCTCTTCACTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCGCGCGGGCGCGGCGTAAGA‑GAAAACCATTGATATAGTCGATTTCCgtgt                                                                        >  2:334780/1‑251 (MQ=255)                                                                                                                                                            gTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACAGCTTCAGTCTCTTCACTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCGCCCGGGCGCGGCGTAAGA‑GAAAACCATTGATATAGTCGATTTCAgtgt                                                                        >  2:132333/1‑251 (MQ=255)                                                                                                                                                                          aCCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGCACCAGCAGATCGATAGTGGTGACAGCTTCAGTCTCTTCACTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGTCGGGTGTTTTCCGGTAAGGCAAACCCCTGCGCCAGGGCGCGGCGTAAGAAGAAAAC‑AATGATATAGTCGCTTTCAgtggtgcgccgcgctcgg                                                          >  1:237009/1‑236 (MQ=255)                                                                                                                                                                                                  ggtgACTTTGAGACCGCCGCGAACCAGCAGATCGATAGTGGTGACAGCTTCAGTCTCGTCACTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCGTTCTTTTTACCATTTCAAACAGGAGGGTGTTTTCCGGTACGGCAATCCCATGCGCGCGGGCGCGGCGTAAGA‑GAAAACCATTGATATAGTCGATTTCAGTGTGGCGCAGCGCGCGGATATCCTGAAACATCGACGAGata                                  <  2:297754/251‑1 (MQ=255)                                                                                                                                                                                                      aCTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACAGCTTCAGTCTCTTCACTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCGCGCGGGCGCGGCGTAAGA‑GAAAACCATTGATATAGTCGATTTCAGTGTGGCGCAGCGCGCGGATATCCTGCAACATCGACGAGATAtttt                              <  1:265735/251‑1 (MQ=255)                                                                                                                                                                                                                cgccgcGAACCAGCAGATCGATAGTGGTGACAGCTTCAGTCTCTTCACTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCGCGCGGGCGCGGCGTAAGA‑GAAAACCATTGATATAGTCGATTTCAGTGTGGCGCAGCGCGCGGATATCCTGCAACATCGACGAGATATTTTCCGCTGTGGc                    <  2:221720/251‑1 (MQ=255)                                                                                                                                                                                                                          cagcagATCGATAGTGGTGACAGCTTCAGTCTCTTCACTTCCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCGCGCGGGCGCGGCGTAAGA‑GAAAACCATTGATATAGTCGATTTCAGTGTGGCGCAGCGCGCGGATATCCTGCAACATCGACGAGATATTTTCCGCTGTGGCATCAATCAcc          <  2:196234/251‑1 (MQ=255)                                                                                                                                                                                                                               gATCGATAGTGGTGACAGCTTCAGTCTCTTCACTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCGCGCGGGCGCGGCGTAAGA‑GAAAACCATTGATATAGTCGATTTCAGTGTGGCGCAGCGCGCGGATATCCTGCAACATCGACGAGATATTTTCCGCTGTGGCATCAATCACCTGCAt     <  1:157613/251‑1 (MQ=255)                                                                                                                                                                                                                                  cGATAGTGGTGACAGCTTCAGTCTCTTCACTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCGCGCGGGCGCGGCGTAAGA‑GAAAACCATTGATATAGTCGATTTCAGTGTGGCGCAGCGCGCGGATATCCTGCAACATCGACGAGATATTTTCCGCTGTGGCATCAATCACCTGCATCAc  <  1:345335/251‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                               AACGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCACCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCACTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCGCGCGGGCGCGGCGTAAGA‑GAAAACCATTGATATAGTCGATTTCAGTGTGGCGCAGCGCGCGGATATCCTGCAACATCGACGAGATATTTTCCGCTGTGGCATCAATCACCTGCATCAC  >  NC_000913/443350‑443825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑