Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	422,868	A→G	P117P (CCA→CCG)	malZ →	maltodextrin glucosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	422,868	0	A	G	97.7%	144.1 / ‑3.9	44	P117P (CCA→CCG)	malZ	maltodextrin glucosidase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (22/21);  total (22/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.63e-01

ATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAGCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTTGGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGGCACGACTGGAGCAGTTTGCCGTCGATGTACCGGATATCGGCCCACAATGGGCTGCGGATCAGATTTTTTATCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGAAGCTGAACAGGATCATGTCTATTACCATCATGCAGCCGGACAAGAGATCATCTTGCGTGACTGGGATGAACCGGTCACGGCGCAGGCGGGCGGATCAACGTTCTATGGCGGCGATCTGG‑ACGGGATAAGCGAAAAACTGCCGTATCTGAAAAAGCTTGGCGTGACAGCG  >  NC_000913/422623‑423105                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                               aTGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAGCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTTGGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGGCACGACTGGAGCAGTTTGCCGTCGATGTACCGGATATCGGCCCGCAAt                                                                                                                                                                                                                                            <  1:102675/250‑1 (MQ=255)      gcGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGGGCGGCGATTGATCTCTCCAGCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTTGGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGGCACGACTGGAGCAGTTTGCCGTCGATGTACCGGATATCGGCCCGCAATGGGCt                                                                                                                                                                                                                                       <  2:369107/250‑1 (MQ=255)            aGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAGCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTTGGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGGCACGACTGGAGCAGTTTGCCGTCGATGTACCGGATATCGGCCCGCAATGGGCTGCGGATc                                                                                                                                                                                                                                <  2:124874/251‑1 (MQ=255)             gAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAGCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTTGGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGGCACGACTGGAGCAGTTTGCCGTCGATGTACCGGATATCGGCCCGCAATGGGCTGCGGATc                                                                                                                                                                                                                                >  2:22180/1‑250 (MQ=255)              aaCACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAGCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTTGGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGGCACGACTGGAGCAGTTTGCCGTCGATGTACCGGATATCGGCCCACAATGGGCTGCGGATCAg                                                                                                                                                                                                                              <  1:317380/251‑1 (MQ=255)                    aTAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCAATTGATCTCTCCAGCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTTGGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGGCACGACTGGAGCAGTTTGCCGTCGATGTACCGGATATCGGCCCGCAATGGGCTGCGGCTCAGAttttt                                                                                                                                                                                                                        >  2:191847/1‑251 (MQ=255)                              aaTGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAGCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTTGGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGGCACGACTGGAGCAGTTTGCCGTCGATGTACCGGATATCGGCCCGCAATGGGCTGCGGATCAGATTTTTTATCAGATCt                                                                                                                                                                                                              >  1:148588/1‑251 (MQ=255)                                      tACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAGCGGACAACCCCGGCGGCGTTACAGTTTCACACTGCTGTGGCACGATCGCCAGCGTTGGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGGCACGACTGGAGCAGTTTGCCGTCGATGTACCGGATATCGGCCCGCAATGGGCTGCGGATCAGATTTTTTATCAGATCTTCCCTGAt                                                                                                                                                                                                      >  1:223890/1‑251 (MQ=255)                                       aCCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAGCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTTGGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGGCACGACTGGAGCAGTTTGCCGTCGATGTACCGGATATCGGCCCGCAATGGGCTGCGGATCAGATTTTTTATCAGATCTTCCCTGATc                                                                                                                                                                                                     >  2:188441/1‑251 (MQ=255)                                        ccGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAGCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTTGGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGGCACGACTGGAGCAGTTTGCCGTCGATGTACCGGATATCGGCCCGCAATGGGCTGCGGATCAGATTTTTTATCAGATCTTCCCTGATCg                                                                                                                                                                                                    >  1:244769/1‑251 (MQ=255)                                                          aGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAGCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTTGGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGGCACGACTGGAGCAGTTTGCCGTCGATGTACCGGATATCGGCCCGCAATGGGCTGCGGATCAGATTTTTTATCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTcc                                                                                                                                                                                  >  1:256427/1‑251 (MQ=255)                                                                    aGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAGCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTTGGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGGCACGACTGGAGCAGTTTGCCGTCGATGTACCGGATATCGGCCCGCAATGGGCTGCGGATCAGAtt                                                                                                                                                                                                                           >  2:55692/1‑200 (MQ=255)                                                                    aGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAGCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTTGGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGGCACGACTGGAGCAGTTTGCCGTCGATGTACCGGATATCGGCCCGCAATGGGCTGCGGATCAGAtt                                                                                                                                                                                                                           <  1:55692/200‑1 (MQ=255)                                                                    aGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAGCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTTGGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGGCACGACTGGAGCAGTTTGCCGTCGATGTACCGGATATCGGCCCGCAATGGGCTGCGGATCAGATTTTTTATCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGAAGCt                                                                                                                                                                        >  2:153944/1‑251 (MQ=255)                                                                        tGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAGCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTTGGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGGCACGACTGGAGCAGTTTGCCGTCGATGTACCGGATATCGGCCCGCAATGGGCTGCGGATCAGATTTTTTATCAGATCTTCCCTGCTCGTTTTGCGCGTAGTCTTCCTCGTGAAGCTGAAc                                                                                                                                                                    >  2:283460/1‑251 (MQ=255)                                                                                     gggCGTGCGGCGATTGATCTCTCCAGCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTTGGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGGCACGACTGGAGCAGTTTGCCGTCGATGTACCGGATATCGGCCCGCAATGGGCTGCGGATCAGATTTTTTATCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGAAGCTGAACAGGATCATGTCTa                                                                                                                                                       <  2:115850/250‑1 (MQ=255)                                                                                               cgATTGATCTCTCCAGCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTTGGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGGCACGACTGGAGCAGTTTGCCGTCGATGTACCGGATATCGGCCCGCAATGGGCTGCGGATCAGATTTTTTATCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGAAGCTGAACAGGATCATGTCTATTACcatcat                                                                                                                                             <  1:188441/251‑1 (MQ=255)                                                                                                   tGATCTCTCCAGCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTTGGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGGCACGACTGGAGCAGTTTGCCGTCGATGTACCGGATATCGGCCCGCAATGGGCTGCGGATCAGATTTTTTATCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGAAGCTGAACAGGATCAGGTCTATTACCAGCATGCa                                                                                                                                          <  2:244769/250‑1 (MQ=255)                                                                                                         ctcCAGCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTTGGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGGCACGACTGGAGCAGTTTGCCGTCGATGTACCGGATATCGGCCCGCAATGGGCTGCGGATCAGATTTTTTATCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGAAGCTGAACAGGATCATGTCTATTACCATCATGCAGCCGGAc                                                                                                                                   >  2:64463/1‑251 (MQ=255)                                                                                                                                                gctgTGGCACGATCGCCAGCGTTGGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGGCACGACTGGAGCAGTTTGCCGTCGATGTACCGGATATCGGCCCGCAATGGGCTGCGGATCAGATTTTTTATCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGAAGCTGAACAGGATCATGTCTATTACCATCATGCAGCCGGACAAGAGATCATCTTGCGTGACTGGGATGAACCGGTCAc                                                                                              <  1:287680/249‑1 (MQ=255)                                                                                                                                                     ggCACGATCGCCAGCGTTGGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGGCACGACTGGAGCAGTTTGCCGTCGATGTACCGGATATCGGCCCGCAATGGGCTGCGGATCAGATTTTTTATCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGAAGCTGAACAGGATCATGTCTATTACCATCATGCAGCCGGACAAGAGATCATCTTGCGTGACTGGGATGAACCGGTCACGGCGCAg                                                                                       <  2:128510/251‑1 (MQ=255)                                                                                                                                                        aCGATCGCCAGCGTTGGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGGCACGACTGGAGCAGTTTGCCGTCGATGTACCGGATATCGGCCCGCAATGGGCTGCGGATCAGATTTTTTATCAGATCTTCCCTGATCGTTTTGcgcg                                                                                                                                                                                           >  2:303020/1‑148 (MQ=255)                                                                                                                                                        aCGATCGCCAGCGTTGGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGGCACGACTGGAGCAGTTTGCCGTCGATGTACCGGATATCGGCCCGCAATGGGCTGCGGATCAGATTTGTTATCAGATCTTCCCTGATCGTTTTGcgcg                                                                                                                                                                                           <  1:303020/148‑1 (MQ=255)                                                                                                                                                                   cGTTGGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGGCACGACTGGAGCAGTTTGCCGTCGATGTACCGGATATCGGCCCGCAATGGGCTGCGGATCAGATTTTTTATCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGAAGCTGAACAGGATCATGTCTATTACCATCATGCAGCCGGACAAGAGATCATCTTGCGTGACTGGGATGAACCGGTCACGGCGCAGgcgggcggggcgac                                                                         >  2:8653/1‑245 (MQ=255)                                                                                                                                                                   cGTTGGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGGCACGACTGGAGCAGTTTGCCGTCGATGTACCGGATATCGGCCCGCAATGGGCTGCGGATCAGATTTTTTATCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGAAGCTGAACAGGATCATGTCTATTACCATCATGCAGCCGGACAAGAGATCATCTTGCGTGACTGGGATGAACCGGTCACGGCGCAGGCGGGCGGATCAAc                                                                         <  1:191847/251‑1 (MQ=255)                                                                                                                                                                                     ggCTTCAGCCGAATGCCGCCGGCACGACTGGAGCAGTTTGCCGTCGATGTACCGGATATCGGCCCGCAATGGGCTGCGGATCAGATTTTTTATCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGAAGCTGAACAGGATCATGTCTATTACCATCATGCAGCCGGACAAGAGATCATCTTGCGTGACTGGGATGAACCGGTCACGGCGCAGGCGGGCGGATCAACGTTCTATGGCGGCGATCt                                                       <  2:148588/251‑1 (MQ=255)                                                                                                                                                                                      gCTTCAGCCGAATGCCGCCGGCACGACTGGAGCAGTTTGCCGTCGATGTACCGGATATCGGCCCGCAATGGGCTGCGGATCAGATTTTTTATCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGAAGCTGAACAGGATCATGTCTATTACCATCATGCAGCCGGACAAGAGATCATCTTGCGTGACTGGGATGAACCGGTCACGGCGCAGgcgggcgggttcaaggttttttggggcgggatt                                                      >  2:219054/1‑226 (MQ=255)                                                                                                                                                                                        ttCAGCCGAATGCCGCCGGCACGACTGGAGCAGTTTGCCGTCGATGTACCGGATATCGGCCCGCAATGGGCTGCGGATCAGATTTTTTATCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGAAGCTGAACAGGATCATGTCTATTACCATCATGCAGCCGGACAAGAGATCATCTTGCGTGACTGGGATGAACCGGTCACGGCGCAGGCGGGCGGGTCAACGGTCTAttgccgcggttttgg                                                    >  2:40788/1‑237 (MQ=255)                                                                                                                                                                                          cAGCCGAATGCCGCCGGCACGACTGGAGCAGTTTGCCGTCGATGTACCGGATATCGGCCCGCAATGGGCTGCGGATCAGATTTTTTATCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGAAGCTGAACAGGATCATGTCTATTACCATCATGCAGCCGGACAAGAGATCATCTTGCGTGACTGGGATGAACCGGTCACGGCGCAGGCGGGCGGATCAACGTTCTATGGCGGCGATCTGG‑Ac                                                  <  2:133968/250‑1 (MQ=255)                                                                                                                                                                                          cAGCCGAATGCCGCCGGCACGACTGGAGCAGTTTGCCGTCGATGTACCGGATATCGGCCCGCAATGGGCTGCGGATCAGATTTTTTATCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGAAGCTGAACAGGATCATGTCTATTACCATCATGCAGCCGGACAAGAGATCATCTTGCGTGACTGGGATGAACCGGTCACGGCGCAGGCGGGCGGATCAACGGTCTATGGGGGCGGTCTgggcg                                                  >  1:365727/1‑248 (MQ=255)                                                                                                                                                                                              cGAATGCCGCCGGCACGACTGGAGCAGTTTGCCGTCGATGTACCGGATATCGGCCCGCAATGGGCTGCGGATCAGATTTTTTATCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGAAGCTGAACAGGATCATGTCTATTACCATCATGCAGCCGGACAAGAGATCATCTTGCGTGACTGGGATGAACCGGTCACGGCGCAGGCGGGCGGATCAACGTTCTATGGCGGCGGTCTGG‑ACggggg                                             >  1:198106/1‑249 (MQ=255)                                                                                                                                                                                               gAATGCCGCCGGCACGACTGGAGCAGTTTGCCGTCGATGTACCGGATATCGGCCCGCAATGGGCTGCGGATCAGATTTTTTATCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGAAGCTGAACAGGATCATGTCTATTACCATCATGCAGCCGGACAAGAGATCATCTTGCGTGACTGGGATGAACCGGTCACGGCGCAGGCGGGGGGGTCAAAGGTCTATGggggcgggcttgggggggt                                              >  2:22209/1‑237 (MQ=255)                                                                                                                                                                                                    ccgccgGCACGACTGGAGCAGTTTGCCGTCGATGTACCGGATATCGGCCCGCAATGGGCTGCGGATCAGATTTTTTATCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGAAGCTGAACAGGATCATGTCTATTACCATCATGCAGCCGGACAAGAGATCATCTTGCGTGACTGGGATGATCCGGTCACGGCTCAGGCGGGCGGATCAACGTTCTAGGGCTTCGCTCTGG‑ACCGGATAAGCGa                                       <  2:166083/251‑1 (MQ=255)                                                                                                                                                                                                             cGACTGGAGCAGTTTGCCGTCGATGTACCGGATATCGGCCCGCAATGGGCTGCGGATCAGATTTTTTATCAGATCTTCCCTGATCGTTTTGCGCTTAGTCTTCCTCGTGAAGCTGAACAGGATCATGTCTATTACCATCATGCAGCCGGACAAGAGATCATCTTGCGTGACTGGGATGAACCGGTCACGGAGCAGGCGGTCGGATCAACGTTCTATGGCGGCGATCTGG‑ACGGGATAAGCGAAAAACTGcc                              <  1:191569/251‑1 (MQ=255)                                                                                                                                                                                                              gACTGGAGCAGTTTGCCGTCGATGTACCGGATATCGGCCCGCAATGGGCTGCGGATCAGATTTTTTATCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGAAGCTGAACAGGATCATGTCTATTACCATCATGCAGCCGGACAAGAGATCATCTTGCGTGACTGGGATGAACCGGTCACGGCGCAGGCGGGCGGATCAACGTTCTATGGCGGCGATCTGG‑ACGGGATAAGCGAAAAACTGCCg                             <  2:63502/251‑1 (MQ=255)                                                                                                                                                                                                                   gAGCAGTTTGCCGTCGATGTACCGGATATCGGCCCGCAATGGGCTGCGGATCAGATTTTTTATCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGAAGCTGAACAGGATCATGTCTATTACCATCATGCAGCCGGACAAGAGATCATCTTGCGTGACTGGGATGAACCGGTCACGGCGCAGGCGGGCGGATCAACGGTCTTTGGCGGCGGTCTGGGAGGGGATAACGGaaaaaacccctatct                         >  2:79188/1‑241 (MQ=255)                                                                                                                                                                                                                           tGCCGTCGATGTACCGGATATCGGCCCGCAATGGGCTGCGGATCAGATTTTTTATCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGAAGCTGAACAGGATCATGTCTATTACCATCATGCAGCCGGACAAGAGATCATCTTGCGTGACTGGGATGAACCGGTCACGGCGCAGGCGGGCGGGTCAACGGTCTATGGCGGCGATCTGG‑ACGGGGTAAACGAAAAAATGCCGGATCTGaaaaaac                >  1:179896/1‑249 (MQ=255)                                                                                                                                                                                                                           tGCCGTCGATGTACCGGATATCGGCCCGCAATGGGCTGCGGATCAGATTTTTTATCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGAAGCTGAACAGGATCATGTCTATTACCATCATGCAGCCGGACAAGAGATCATCTTGCGTGACTGGGATGAACCGGTCACGGCGCAGGCGGGCGGATCAACGTTCTATGGCGGCGATCTGG‑ACGGGATAAGCGAAAAACTGCCGTATCTGAAAAAg                 <  1:190586/250‑1 (MQ=255)                                                                                                                                                                                                                               gTCGATGTACCGGATATCGGCCCGCAATGGGCTGCGGATCAGATTTTTTATCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGAAGCTGAACAGGATCATGTCTATTACCATCATGCAGCCGGACAAGAGATCATCTTGCGTGACTGGGATGAACCGGTCACGGCGCAGGCGGGAGGGTTCACGGTCTATGGCGGCGATTTAG‑ACGGGAGAAACGAAAAACTGCCgcaactaaaaacacctgg            >  1:262781/1‑234 (MQ=255)                                                                                                                                                                                                                                  gATGTACCGGATATCGGCCCGCAATGGGCTGCGGATCAGATTTTTTATCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGAAGCTGAACAGGATCATGTCTATTACCATCATGCAGCCGGACAAGAGATCATCTTGCGTGACTGGGATGAACCGGTCACGgc                                                                                           <  1:332746/170‑1 (MQ=255)                                                                                                                                                                                                                                  gATGTACCGGATATCGGCCCGCAATGGGCTGCGGATCAGATTTTTTATCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGAAGCTGAACAGGATCATGTCTATTACCATCATGCAGCCGGACAAGAGATCATCTTGCGTGACTGGGATGAACCGGTCACGgc                                                                                           >  2:332746/1‑170 (MQ=255)                                                                                                                                                                                                                                     ggaCCGGATATCGGCCCGCAATGGGCTGCTGATCATATTTTTTATCAGATCGTCCCTGATCGTTTTGCGCGTAGTATTCCGCGTGAAGCTGAACAGGATCATGTCTATTACCATCATGCAGCCGGACAAGAGATCATCTTGCGTGACTGGGATGAACCGGTCACGGAGCAGGCGGGCGGATCAACGTTCTATGGCGGCGATCTGG‑ACGGGATAAGCGAAAAACTGCCGTATCTGAAAAAGCTTGGCGTGAc      <  1:201521/249‑1 (MQ=255)                                                                                                                                                                                                                                       aCCGGATATCGGCCCGCAATGGGCTGCGGATCAGATTTTTTATCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGAAGCTGAACAGGATCATGTCTATTACCATCATGCAGCCGGACAAGAGATCATCTTGCGTTACTGGGATGAACCGGTCACGGCGCAGGCGGGCGGATCAACGTTCTATGGCGGCGATCTGG‑ACGTGATAAGCGAAAAACTGCCGTATCTGAAAAAGCTTGGCGTGACAg    <  1:44181/251‑1 (MQ=255)                                                                                                                                                                                                                                         cGGATATCGGCCCGCAATGGGCTGCGGATCAGATTTTTTATCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGAAGCTGAACAGGATCATGTCTATTACCATCATGCAGCCGGACAAGAGATCATCTTGCGTGACTGGGATGAACCGGTCACGGCGCAGGCGGGCGGATCAACGTTCTATGGCGGCGATCTGG‑ACGGGATAAGCGAAAAACTGCCGTATCTGAAAAAGCTTGGCGTGACAgcg  <  1:22209/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                               ATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAGCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTTGGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGGCACGACTGGAGCAGTTTGCCGTCGATGTACCGGATATCGGCCCACAATGGGCTGCGGATCAGATTTTTTATCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGAAGCTGAACAGGATCATGTCTATTACCATCATGCAGCCGGACAAGAGATCATCTTGCGTGACTGGGATGAACCGGTCACGGCGCAGGCGGGCGGATCAACGTTCTATGGCGGCGATCTGG‑ACGGGATAAGCGAAAAACTGCCGTATCTGAAAAAGCTTGGCGTGACAGCG  >  NC_000913/422623‑423105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑