Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	65,316	T→A	I155I (ATA→ATT)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	65,316	0	T	A	97.5%	119.4 / NA	40	I155I (ATA→ATT)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (18/21);  minor base C (1/0);  total (19/21)
Fisher's exact test for biased strand distribution p-value = 4.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACTGCGGGCGGCTGGCGACGTATTCCAGTTCGAAATCAAGCGAGGAGGCGTCGCCATTCTCCGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAAT‑ATCTATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGG‑TTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTG  >  NC_000913/65072‑65561                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       cATGTTTTTGCAGCATTCGCAGATCGAACTGCACCCCGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGGGGTTTCACT‑ATCaataaa                                                                                                                                                                                                                                                   >  2:51747/1‑248 (MQ=255)  aTGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGGGGGTTCAAT‑ATCAATAGaa                                                                                                                                                                                                                                                  >  1:350839/1‑250 (MQ=255)      ttttGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAAT‑ATCAATAGAAAccc                                                                                                                                                                                                                                              <  2:22251/250‑1 (MQ=255)         tGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGCTCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAAT‑ATAAATAGAAACCCACtt                                                                                                                                                                                                                                          >  1:338818/1‑251 (MQ=255)               aTTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGGTTCAAT‑CTCAATAGAAAACCACTTGCgcgg                                                                                                                                                                                                                                    >  2:55624/1‑251 (MQ=255)                         cGAACTGCCCCACGTTAAAACCGCTGATCACATCAGAATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAGCTGCGGGCGGCTGGCGACGTATTCCCGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCAAGCATATAAACGATACGCTGCCCGCAGCCTTCCAGGCAGATGCAGTACAGCTCACCGTGGCGGGGGGTGTCAATTAT‑AAAAGCAACCCACTTGAACGGCGGagcat                                                                                                                                                                                                                            >  2:16897/1‑245 (MQ=255)                         cGAACTGCACCACGTTCCAACCGATGATCACATCCGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCCAT‑ATCAATAGAAACCCACTTGAGCGGCGGACGAt                                                                                                                                                                                                                            >  1:276589/1‑249 (MQ=255)                                           aaCCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAAT‑ATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGG‑TTTCAGa                                                                                                                                                                                                       <  1:176540/251‑1 (MQ=255)                                                   atCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAAT‑ATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGG‑TTTCAGACGGGCAtt                                                                                                                                                                                               <  2:307096/251‑1 (MQ=255)                                                     cacaTCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGGTTCAAT‑ATCAATAGAAACCCACTTTAGCGGCGGGCGATAGTCGGGATGCGG‑GTTCAGACGGGCATTa                                                                                                                                                                                              >  1:82540/1‑250 (MQ=255)                                                       caTCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACTGTGGCGGGTGGTTTTACT‑ATCACTAAAAAACCCCGTTAGCGGGGGGCGAGAGGCTGGATGCGG‑GTTTAGACAGGCATTAACg                                                                                                                                                                                           >  2:285842/1‑251 (MQ=255)                                                             gATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGGTTCAAT‑ATCAATAGAAACCCACCTGAGCGGCGGGCGATAGTCGGGAGGCGG‑GTTTAGAAGGGCATTAACGATAgcg                                                                                                                                                                                     >  2:70500/1‑249 (MQ=255)                                                                 gTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGAGTCGCCATTCTCTGACCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCCGGCCGATGCAGTACAGCTCACCGTGGCGGGAGGGTTCAAC‑ATCCATAGAAAACCCCTTGACCGGCTGAAGATAGTATGGATACGG‑GTTAAGACGGGGAGTAACGATTGCGCCAt                                                                                                                                                                                 >  1:353067/1‑251 (MQ=255)                                                                         cAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGGTTCAAT‑ATCAATAGAAACCCACTTGAGCGGCGGGCGGTAGGTGGGGTGCGG‑GTTTAGACGGGCAATAAAGATAggccccttgtggat                                                                                                                                                                          >  2:11701/1‑237 (MQ=255)                                                                          aaaCCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGGGGGTTTAAT‑ATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGG‑TTTCAGACGGGCATTAACGATAGCGCCATTGTGCatat                                                                                                                                                                        >  1:297806/1‑251 (MQ=255)                                                                                 gCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAAT‑ATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGG‑TTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTc                                                                                                                                                                 <  1:11419/251‑1 (MQ=255)                                                                                     tGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGGTTCAAT‑ATCAATAGAAAACCACTTGAGCGGCGGGCGGTAGGCCGGCTGCGGGTTTC‑GACGGGCGTTAACGAAAGCGCCCTTGTTCATATCAccccccacc                                                                                                                                                             >  2:282413/1‑245 (MQ=255)                                                                                      gAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGGTTCCAT‑ATCAATAGAAACCCACTTGAGAGGCGGGCGGTAGGCGGGGTGCGG‑GTTTAAACGGGCATTTAAGATAGAGCCATTGGTCATATCACCCTCGAccc                                                                                                                                                            >  2:126449/1‑251 (MQ=255)                                                                                                       gcgggcggCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAAT‑ATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGG‑TTTCAGACGGGCATTAACGATAGCGACATTGTGCATATCACCCTCGACCCACTCCGGTGAGGTGAt                                                                                                                                            <  1:269001/250‑1 (MQ=255)                                                                                                                  gCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCATGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAAT‑ATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGG‑TTTCAGACGGGCATTAACGATATCGCCAGTGTGCATATCACCCGCGACCCACACCGGTGAGGTGATAAACCGCTCCAt                                                                                                                                <  2:47717/251‑1 (MQ=255)                                                                                                                   cGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAAT‑ATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGG‑TTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGGGAGGGGATAAACCGCTCCATc                                                                                                                               >  1:131396/1‑251 (MQ=255)                                                                                                                                  caaaaTCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAAT‑ATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGG‑TTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGc                                                                                                                <  1:70500/249‑1 (MQ=255)                                                                                                                                  caaaaTCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAAT‑ATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGG‑TTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGc                                                                                                                <  2:276589/249‑1 (MQ=255)                                                                                                                                                  ggCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAAT‑ATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGG‑TTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCt                                                                                                <  1:335632/251‑1 (MQ=255)                                                                                                                                                        gCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAAT‑ATCAATAGAAACCCACtt                                                                                                                                                                                                                                          <  2:374411/108‑1 (MQ=255)                                                                                                                                                        gCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAAT‑ATCAATAGAAACCCACtt                                                                                                                                                                                                                                          >  1:374411/1‑108 (MQ=255)                                                                                                                                                              ctctggCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGGGGTTTCAAT‑ATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGG‑GTTCAGACGGGCATTAACGATAGCGCCCTTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAAACGCTCCATCAGATAGCGTTCTGGCGGGGGCCCCACGGCCTCCTAGACGGtg                                                                                     >  1:250738/5‑249 (MQ=255)                                                                                                                                                                ctggCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAAT‑ATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGG‑TTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTaa                                                                                    <  1:51747/247‑1 (MQ=255)                                                                                                                                                                        aGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCCAT‑ATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGG‑TTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCCTCAGATAGCGTTCTGGCGGGGGCACATCGGCCCCGTAGACGGTAACGCCCCCTTc                                                                          >  1:372067/1‑251 (MQ=255)                                                                                                                                                                                   gATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAAT‑ATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGG‑TTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCt                                                               <  1:15192/251‑1 (MQ=255)                                                                                                                                                                                    aTGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAAT‑ATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGG‑TTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCtt                                                              <  2:131396/251‑1 (MQ=255)                                                                                                                                                                                        gcTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAAT‑ATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGG‑TTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCTCCTTCACGCAGGCGCTTTTCg                                                          <  1:271331/251‑1 (MQ=255)                                                                                                                                                                                             ccGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAAT‑ATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGG‑TTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAAtt                                                     <  1:361571/251‑1 (MQ=255)                                                                                                                                                                                                  gCCTTCCAGGCCGAGGCAGTACAGCTCACCGTGGCGGGTGGTTTCAAT‑ATCAATAGAAACCCACTTGAGCGGCGGACGAGAGTCGGGATGCGG‑TTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATtcatca                                                <  2:366433/251‑1 (MQ=255)                                                                                                                                                                                                        cAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAAT‑ATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGG‑TTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATaaa                                                                                                                                         <  2:57193/156‑1 (MQ=255)                                                                                                                                                                                                        cAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAAT‑ATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGG‑TTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGGGATaaa                                                                                                                                         >  1:57193/1‑156 (MQ=255)                                                                                                                                                                                                                 gCAGTACAGCTCACCGTGGCGGGTGGTTTCAAT‑ATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGG‑TTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGgcg                                  <  2:297806/250‑1 (MQ=255)                                                                                                                                                                                                                               cGTGGCGGGTGGTTTCAAT‑ATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGG‑TTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCa                   <  1:77123/251‑1 (MQ=255)                                                                                                                                                                                                                                              cAAT‑ATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGG‑TTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCgg    <  1:66568/251‑1 (MQ=255)                                                                                                                                                                                                                                              cAAT‑ATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGG‑TTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGAGGGGCGCGACAGTAAAGGCCATACACCGGCTGGCgg    >  1:349080/1‑251 (MQ=255)                                                                                                                                                                                                                                                at‑atCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGG‑TTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTg  <  2:350839/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       CATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACTGCGGGCGGCTGGCGACGTATTCCAGTTCGAAATCAAGCGAGGAGGCGTCGCCATTCTCCGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAAT‑ATCTATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGG‑TTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTG  >  NC_000913/65072‑65561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑