Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	267,147	T→C	intergenic (‑594/+37)	yafY ← / ← yafZ	inner membrane lipoprotein YafY/DUF932 domain‑containing protein YafZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	267,147	0	T	C	100.0%	134.3 / NA	43	intergenic (‑594/+37)	yafY/yafZ	inner membrane lipoprotein YafY/DUF932 domain‑containing protein YafZ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/22);  total (21/22)

CAGACGGCTGATTATCAGTGACAGCCTGACAGCCAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGTGGTGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTGGCATTACGGCCACTGAGCCCGCCCTTAATCAGGTTCTCCTGAATACGCTGGTACGTGGTCCACAGGTCATTGCTCTCATCCTGCCAGCGGCGAGGGGAG  >  NC_000913/266902‑267391                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     caGACGGCTGATTATCAGTGACAGCCTGACAGCCAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGCGgtgt                                                                                                                                                                                                                                                 <  1:351170/251‑1 (MQ=255)             tATCAGTGACAGCCTGACAGCCAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGGTAACTGACTGAAAATGATGTGATTTCTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGCGGTGTCCGATATTAAc                                                                                                                                                                                                                                      <  2:286205/250‑1 (MQ=255)                         cctgacagccAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCTTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGCGGTGTCCGATATTAACAAAGCAGCAACAt                                                                                                                                                                                                                         <  2:191009/251‑1 (MQ=255)                             acagccAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGCGGTGTCCGATATTAACAAAGCAGCAACATTaaa                                                                                                                                                                                                                     <  2:260862/251‑1 (MQ=255)                                  cagccGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGCGGTGTCCGATATTAACAAAGCAGCAACATTAAACGGTc                                                                                                                                                                                                                <  2:60754/251‑1 (MQ=255)                                       ggTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGCGGTGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGc                                                                                                                                                                                                           >  1:301989/1‑251 (MQ=255)                                         tCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGCGGTGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGc                                                                                                                                                                                                           >  1:95789/1‑249 (MQ=255)                                              gcggcgTGCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTACTTTAAAGAGGGTGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTTCGATGCGGACATGCGGTGTTCGATATTAACAAAGCAGCAACAGTAAACGGTCACTGCAGTTGCg                                                                                                                                                                                                    <  1:19652/251‑1 (MQ=37)                                                 gcgTTCTGCCTGTGTCATGAGAAGCTCTCCGAGAAAGTTAACTGACTGAAACTGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGCGGACCGTCAGCACGGCGCGATGCGGACAGGCGGTGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCCGTTGCGTg                                                                                                                                                                                                  >  2:222734/1‑250 (MQ=255)                                                      cTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTAACTGACTAAAACTGACGTGATTAATTTCAAGAGGGGGTCGAACAGGGAATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGCGGTTACAGGCCTTCCCTCGGTGAGTGAGGACAGTCAGCACGGTGCGATGCGGACAGGCGGTGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAgg                                                                                                                                                                                            >  1:51988/1‑251 (MQ=255)                                                          cTGTGTCATGAGACGTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGCGGTGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCtt                                                                                                                                                                                        >  1:295347/1‑251 (MQ=255)                                                                cATGAGACGTTCTCCGTGAAAGTTAACTGACTGAACATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGCGGTGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGcc                                                                                                                                                                                   >  1:108130/1‑250 (MQ=255)                                                                    agaCGTTCTCCGTGAAAGTTAACTGACTGAAACTGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACAACACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGCGGTGTCCGATATTAACAAAGAAGCAACATTAAACCGTCACTGCAGTTTCGTGAGCAGGGCTTACGCCATCAc                                                                                                                                                                              >  1:347922/1‑251 (MQ=255)                                                                     gaCGTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGCGGTGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCAcc                                                                                                                                                                             <  2:254170/251‑1 (MQ=255)                                                                                   aaGTTAACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGCGGTGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGtt                                                                                                                                                               >  1:139165/1‑251 (MQ=255)                                                                                            gactgaAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGCGGTGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTTCGCCATCACCCACAGTGCCTGGTGAAGTTTc                                                                                                                                                        <  2:58747/249‑1 (MQ=255)                                                                                            gactgaAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTAGGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGCGGTGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTGCCGCCATCACCCACATTGCCCGGTTAAGTTTCAc                                                                                                                                                      <  2:133756/251‑1 (MQ=255)                                                                                                         gtgATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGCGGTGTCCGATATTAACAAAGCAGCAACATTAAACGGGCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGTTGAAGTTTCACGGCCCCGCCGAtc                                                                                                                                         >  2:206962/1‑250 (MQ=255)                                                                                                              tACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGCGGTGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACcgc                                                                                                                                     <  1:139082/250‑1 (MQ=255)                                                                                                                 tttAAAGAGGGGGTCGGACAGGGTATGGACACCACAGACACTATTTTTCATTCCTGCAAAAGCCAGAGGCAGAGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGCGGTGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAAc                                                                                                                                 >  1:233827/1‑251 (MQ=255)                                                                                                                      agagGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGCGGTGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGccc                                                                                                                            >  2:141972/1‑249 (MQ=255)                                                                                                                               cGGACAGGGTATGGACACCACAGAAACTAGTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTCCAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGCGGTGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTAtgt                                                                                                                    <  2:295849/250‑1 (MQ=255)                                                                                                                                ggACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGCGGTGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGa                                                                                                                  <  2:1882/251‑1 (MQ=255)                                                                                                                                         aTGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGCGGTGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGtcctcc                                                                                                         >  1:279534/1‑251 (MQ=255)                                                                                                                                               cccccaGAAACTATTTTCCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGATGACCGTCAGCACGGTGCGATGCTGACAGGCGGTGTCCTATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCTCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTGGc                                                                                                   <  2:324004/247‑1 (MQ=255)                                                                                                                                                    aGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGCCAGCACGGTGCGATGCGGACAGGCGGTGACCGATATTAACAAAGCAGCAAAATTAAACGGTCACTGCAGTTGCGAGAGTAGGGCTTCCGCCATCACCCACAGTGCCCGGATAAGTTTCACGTCCCCGTCGATACCGCGAACAGCCCGTGTATGTGATCATCCTCCTTTGGCATTAc                                                                                              >  2:193537/1‑251 (MQ=255)                                                                                                                                                        aCTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGCGGTGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTGGCATTACGGcc                                                                                          >  2:120776/1‑251 (MQ=255)                                                                                                                                                                       tGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGCGGTGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTGGCATTACGGCCACTGAGCCCGCCCtt                                                                           >  1:253080/1‑251 (MQ=255)                                                                                                                                                                        gCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGCGGTGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTGGCATTACGGCCACTGAGCCCGCCCtt                                                                           <  1:73241/250‑1 (MQ=255)                                                                                                                                                                        gCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGCGGTGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTGGCATTACGGCCACTGAGCCCGCCCtt                                                                           <  1:268006/250‑1 (MQ=255)                                                                                                                                                                           aaaGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGCGGTGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCGCCTTTGGCATTACGGCCACTGAGCCCGCCCTTAATc                                                                       <  2:372373/251‑1 (MQ=255)                                                                                                                                                                                 agagGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGCGGTGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGCTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTGGCATTACGGCCACTGAGCCCGCCCTTACTCAGGTtc                                                                 >  2:70175/1‑251 (MQ=255)                                                                                                                                                                                          gggggTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGCGGTGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTGGCATTACGGCCACTGAGCCCGCCCTTAATCAGGTTCTCCTGAAt                                                         >  1:118618/1‑250 (MQ=255)                                                                                                                                                                                                  cAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGCGGTGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTGGCATTACGGCCACTGAGCCCGCCCTTAATCAGGTTCTCCTGAATACGCTGGTa                                                <  2:333494/251‑1 (MQ=255)                                                                                                                                                                                                         tCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGCGGTGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTGGCATTACGGCCACTGAGCCCGCCCTTAATCAGGTTCTCCTGAATACGCTGGTACGTGGt                                          <  1:120776/250‑1 (MQ=255)                                                                                                                                                                                                                                aGCACGGTGCGATGCGGACAGGCGGTGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTGGCATTACGGCCACTGAGCCCGCCCTTAATCAGGTTCTCCTGAATACGCTGGTACGTGGTCCACAGGTCATTGCTCTCATCCTg                  >  1:92338/1‑251 (MQ=255)                                                                                                                                                                                                                                aGCACGGTGCGATGCGGACAGGCGGTGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTGGCATTACGGCCACTGAGCCCGCCCTTAATCAGGTTCTCCTGAATACGCTGGTACGTGGTCCACAGGTCATTGCTCTCATCCTg                  >  1:328871/1‑251 (MQ=255)                                                                                                                                                                                                                                  cccggtgcggtgCGGACAGGCGTTGTCCGATATTAACAAAGCATCAACATTAAACGGGCGCTGCTGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGTTATGTGACCGTCCTCCTTGCGCATTACGGCCACTGCGCCCGCCCTTAATCAGGTTCTCCTGAATACGCTGGTACGTGGTCCACAGGTCATGGCTCTCATCCttcc                <  2:47912/249‑4 (MQ=255)                                                                                                                                                                                                                                   ccGGTGCGATGCGGACAGGCGGTGTCCGATATTAACAAAGCAGCAAAATTAAACGGTCACGGCAGTTGCGTGAGCAGGGCTCCCGCCATCACCCACAGGGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCGTTGGCATTACGGCCACTGAGCCCGCCCTTAATCAGGTTCTCCTGAATACGCTGGTACGTGGTCCACAGGTCATTGCTCTCATCCTGCCa               <  2:300832/250‑1 (MQ=255)                                                                                                                                                                                                                                      gTGCGATGCGGACAGGCGGTGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTGGCATTACGGCCACTGAGCCCGCCCTTAATCAGGTTCTCCTGAATACGCTGGTACGTGGTCCACAGGTCATTGCTCTCATCCTGCCAgcg            <  1:82222/251‑1 (MQ=255)                                                                                                                                                                                                                                        gCGATGCGGTTAGGCGGTGTACGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTGCCGCTATCACCCACAGTGCCCGGCTAATTTTCACTTTCCGGTCGAGACCGCGAACCGCACGGGTAGGTGACCGTCCTCCTTTGGCATGACGGCCAGTTAGCCCGCCCTTAATCAGTTCCGCCTGAATGCGCTGGTACGTGGTCCACAGGTCATCGCTCTGATCCTTCCggcggc          <  2:355560/251‑1 (MQ=255)                                                                                                                                                                                                                                           aTGCGGACAGGCGGTGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTGGCATTACGGCCACTGAGCCCGCCCTTAATCAGGTTCTCCTGAATACGCTGGTACGTGGTCCACAGGTCATTGCTCTCATCCTGCCAgcggcg         >  1:207828/1‑249 (MQ=255)                                                                                                                                                                                                                                                  cAGGCGGTGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTGGCATTACGGCCACTGAGCCCGCCCTTAATCAGGTTCTCCTGAATACGCTGGTACGTGGTCCACAGGTCATTGCTCTCATCCTGCCAGCGGCGAGGGgag  >  1:129175/1‑249 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     CAGACGGCTGATTATCAGTGACAGCCTGACAGCCAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGCGGACAGGTGGTGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTGGCATTACGGCCACTGAGCCCGCCCTTAATCAGGTTCTCCTGAATACGCTGGTACGTGGTCCACAGGTCATTGCTCTCATCCTGCCAGCGGCGAGGGGAG  >  NC_000913/266902‑267391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑