Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	227,091	G→A	noncoding (1333/2904 nt)	rrlH →	23S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	227,091	0	G	A	94.7%	39.7 / ‑6.6	19	noncoding (1333/2904 nt)	rrlH	23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (9/9);  total (10/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACTGGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGGCAGGGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACAGGTTAATATTCCTGTACTTGGTGTTACTGCG‑AAGGGGGG‑ACGGAGAAGGCTATGTTGGCCGGGCGACGGTTGTCCCGGTTTAAGCG  >  NC_000913/226860‑227231                                                                                                                                                                                                                                        |                                                                                                                                               cACTGGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGAGCAGGGTGAGTCGACCCCt                                                                                                                             >  1:59585/1‑251 (MQ=21)  aCTGGTCGAGTAGGCCTGCGCGGAAGAAGTAACGGCGCAAAACCATGCACAGAAGCTGCGGCAGAGAGGCTTATGCGGTGGTGGGAAGGGGAGCGTTCTGTAAGCCAGTGAAGGTGTACTGGGAGGTCTGCTGGAGGAATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGAGGGTGAAAAGCCCGCTCGCCGGAAGACCCAGGGATCCAGTACCACGTTAATCGGAGCAGGGGGAGTCGAccccg                                                                                                                             >  2:60710/1‑249 (MQ=11)               gCCTGCGCGGAAGATGTAACGGGGCTAAACCATGCACCGAAGCTGCGGCCGCGACGCTTATGCGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGAGCAGGGTGAGTCGACCCCTAAGGCGAGGCCGa                                                                                                                >  2:92685/1‑250 (MQ=25)                  tGCGCCGAAGATGTAAAGGGGATAGACGATGCACCGCCGCTGCGGCAGCGAAGCTTCTGGGCTGCTGGGTAGGGGCGCGTTCTGTTAGCCTGTGAAGGTGTACTGTGAGGGATGCTGGGGGAATCGGAAGTGAGAATGCTGCCATCAGAAACGCTAAATCGGGTGAAAAGCCAGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATAGGGGCAGGGTGAGTCGACCCCTACGGCGAGGCCGACAgg                                                                                                            >  2:371456/1‑251 (MQ=2)                       ggAAGATGTCACGGGGCTAAACCATGCACCCAAGCTGCGGCAGCGACGCTTAGGCGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGACCAACGTTAATCGGAGCAGGGTGAGTCGAACCCTAAGGCGAGGCCGAAAGGCGTAg                                                                                                       <  2:195514/251‑1 (MQ=255)                              gTAACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGAGCAGGGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATgg                                                                                                <  2:51178/251‑1 (MQ=21)                                     ggCTAAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCGGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGAGCAGGGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACAg                                                                                         <  2:371369/251‑1 (MQ=21)                                                    cGAAGCTGCGGCAGCGACGCTTATGCGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGAGCAGGGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAg                                                                                                       <  1:299480/222‑1 (MQ=25)                                                    cGAAGCTGCGGCAGCGACGCTTATGCGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGAGCAGGGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAg                                                                                                       >  2:299480/1‑222 (MQ=25)                                                    cGAAGCTGCGGCAGCGACGCTTATGCGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGAGCAGGGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACAGGTTAATATTCCTGt                                                                           >  1:78863/1‑250 (MQ=25)                                                    cGAAGCTGCGGCAGCGACGCTTATGCGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGAGCAGGGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACAGGTTAATATTCCTGTa                                                                          <  1:174715/251‑1 (MQ=21)                                                           gCGGCAGCGACGCTTATGCGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGAGCAGGGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACAGGTTAATATTCCTGTACTTgg                                                                     <  2:78354/249‑1 (MQ=25)                                                                     cGCTTATGCGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGAGCAGGGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACAGGTTAATATTCCTGTACTTGGTGTTACTGCG‑a                                                         >  2:65886/1‑250 (MQ=25)                                                                              gttgttGGGAGGGGGGGCTTTCTGTAAGCCGGTAAAGGTGTACTGTGAGGTATGCTGGAGGTAACAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCGGTCCAACGTTAAGCGGAGCAGGGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACAGGTTAATATTCCTGTACTTGGTGTTACTGCG‑AAgggggggac                                               <  2:88960/251‑4 (MQ=11)                                                                                     ggTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGAGCAGGGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACAGGTTAATATTCCTGTACTTGGTGTTACTGCG‑AAGGGGGGGACGgaga                                          >  2:283560/1‑249 (MQ=18)                                                                                                            tgtgAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGAGCAGGGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACAGGTTAATATTCCTGTACTTGGTGTTACTGCG‑AAGGGGGGGACGGAGAAGGCTATGTTGGCCGGGCGACGGtt                 >  2:207922/1‑251 (MQ=18)                                                                                                               gAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGAGCAGGGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACAGTTTAATAGTCCTTTACTTGGTGTTACGGCGTAGGGGGGG‑ACGGAGAAGGCTATGTTGGCCGGGCGACGGTTGt               <  1:171572/250‑1 (MQ=11)                                                                                                                    tgtACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGAGCAGGGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACAGGTTAATATTCCTGTACTTGGTGTTACTGCG‑AAGGGGGGGACGGAGAAGGCTATGTTGGCCGGGCGACGGTTGTCCCGGt         >  1:325228/1‑251 (MQ=18)                                                                                                                            gAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGAGCAGGGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACAGGTTAATATTCCTGTACTTGGTGTTACTGCG‑AAGGGGGGGACGGAGAAGGCTATGTTGGCCGGGCGACGGTTGTCCCGGTTTAAGCg  <  1:31798/250‑1 (MQ=14)                                                                                                                                                                                                                                        |                                                                                                                                               CACTGGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGGCAGGGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACAGGTTAATATTCCTGTACTTGGTGTTACTGCG‑AAGGGGGG‑ACGGAGAAGGCTATGTTGGCCGGGCGACGGTTGTCCCGGTTTAAGCG  >  NC_000913/226860‑227231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑