Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	65,232	C→T	P183P (CCG→CCA)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	65,232	0	C	T	97.3%	122.6 / NA	37	P183P (CCG→CCA)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (22/14);  minor base G (0/1);  total (22/15)
Fisher's exact test for biased strand distribution p-value = 4.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

AAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCGGAAGACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACTGCGGGCGGCTGGCGACGTATTCCAGTTCGAAATCAAGCGAGGAGGCGTCGCCATTCTCCGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAAT‑ATCTATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGG‑TTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGACGCACATCGGCCT  >  NC_000913/64986‑65467                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                              aaaGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGcc                                                                                                                                                                                                                                           >  2:301426/1‑251 (MQ=255)    gACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGcccc                                                                                                                                                                                                                                         >  1:336955/1‑250 (MQ=255)                      gTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCt                                                                                                                                                                                                                      <  1:301426/251‑1 (MQ=255)                               ccACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGc                                                                                                                                                                                                             >  1:22251/1‑251 (MQ=255)                                    ccAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTc                                                                                                                                                                                                         <  1:60264/250‑1 (MQ=255)                                          cactgttatCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGATCGCGGAGGCGTCGCCATTCTCTGTTCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGcc                                                                                                                                                                                                   <  1:353057/245‑1 (MQ=255)                                                      gccccagccGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGCTGCAGTACAGc                                                                                                                                                                                      >  2:77123/9‑251 (MQ=255)                                                                          cGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACGGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCTACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCGGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTc                                                                                                                                                                  <  1:301592/251‑1 (MQ=255)                                                                                 gCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGGGGCGGCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGGGGCGTATGGTTTCAat‑attc                                                                                                                                                          >  1:74471/1‑249 (MQ=255)                                                                                  cTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAg                                                                                                                                                                                                      <  2:360164/207‑1 (MQ=255)                                                                                  cTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAg                                                                                                                                                                                                      >  1:360164/1‑207 (MQ=255)                                                                                       cATGTTTTTGCAGCATTCGCAGATCGAACTGCACCCCGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGGGGTTTCACT‑ATCaataaa                                                                                                                                                     >  2:51747/1‑248 (MQ=255)                                                                                        aTGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGGGGGTTCAAT‑ATCAATAGaa                                                                                                                                                    >  1:350839/1‑250 (MQ=255)                                                                                            ttttGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAAT‑ATCAATAGAAAccc                                                                                                                                                <  2:22251/250‑1 (MQ=255)                                                                                               tGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGCTCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAAT‑ATAAATAGAAACCCACtt                                                                                                                                            >  1:338818/1‑251 (MQ=255)                                                                                                     aTTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGGTTCAAT‑CTCAATAGAAAACCACTTGCgcgg                                                                                                                                      >  2:55624/1‑251 (MQ=255)                                                                                                               cGAACTGCCCCACGTTAAAACCGCTGATCACATCAGAATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAGCTGCGGGCGGCTGGCGACGTATTCCCGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCAAGCATATAAACGATACGCTGCCCGCAGCCTTCCAGGCAGATGCAGTACAGCTCACCGTGGCGGGGGGTGTCAATTAT‑AAAAGCAACCCACTTGAACGGCGGagcat                                                                                                                              >  2:16897/1‑245 (MQ=255)                                                                                                               cGAACTGCACCACGTTCCAACCGATGATCACATCCGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCCAT‑ATCAATAGAAACCCACTTGAGCGGCGGACGAt                                                                                                                              >  1:276589/1‑249 (MQ=255)                                                                                                                                 aaCCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAAT‑ATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGG‑TTTCAGa                                                                                                         <  1:176540/251‑1 (MQ=255)                                                                                                                                         atCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAAT‑ATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGG‑TTTCAGACGGGCAtt                                                                                                 <  2:307096/251‑1 (MQ=255)                                                                                                                                           cacaTCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGGTTCAAT‑ATCAATAGAAACCCACTTTAGCGGCGGGCGATAGTCGGGATGCGG‑GTTCAGACGGGCATTa                                                                                                >  1:82540/1‑250 (MQ=255)                                                                                                                                             caTCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACTGTGGCGGGTGGTTTTACT‑ATCACTAAAAAACCCCGTTAGCGGGGGGCGAGAGGCTGGATGCGG‑GTTTAGACAGGCATTAACg                                                                                             >  2:285842/1‑251 (MQ=255)                                                                                                                                                   gATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGGTTCAAT‑ATCAATAGAAACCCACCTGAGCGGCGGGCGATAGTCGGGAGGCGG‑GTTTAGAAGGGCATTAACGATAgcg                                                                                       >  2:70500/1‑249 (MQ=255)                                                                                                                                                       gTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGAGTCGCCATTCTCTGACCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCCGGCCGATGCAGTACAGCTCACCGTGGCGGGAGGGTTCAAC‑ATCCATAGAAAACCCCTTGACCGGCTGAAGATAGTATGGATACGG‑GTTAAGACGGGGAGTAACGATTGCGCCAt                                                                                   >  1:353067/1‑251 (MQ=255)                                                                                                                                                               cAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGGTTCAAT‑ATCAATAGAAACCCACTTGAGCGGCGGGCGGTAGGTGGGGTGCGG‑GTTTAGACGGGCAATAAAGATAggccccttgtggat                                                                            >  2:11701/1‑237 (MQ=255)                                                                                                                                                                aaaCCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGGGGGTTTAAT‑ATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGG‑TTTCAGACGGGCATTAACGATAGCGCCATTGTGCatat                                                                          >  1:297806/1‑251 (MQ=255)                                                                                                                                                                       gCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAAT‑ATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGG‑TTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTc                                                                   <  1:11419/251‑1 (MQ=255)                                                                                                                                                                           tGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGGTTCAAT‑ATCAATAGAAAACCACTTGAGCGGCGGGCGGTAGGCCGGCTGCGGGTTTC‑GACGGGCGTTAACGAAAGCGCCCTTGTTCATATCAccccccacc                                                               >  2:282413/1‑245 (MQ=255)                                                                                                                                                                            gAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGGTTCCAT‑ATCAATAGAAACCCACTTGAGAGGCGGGCGGTAGGCGGGGTGCGG‑GTTTAAACGGGCATTTAAGATAGAGCCATTGGTCATATCACCCTCGAccc                                                              >  2:126449/1‑251 (MQ=255)                                                                                                                                                                                             gcgggcggCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAAT‑ATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGG‑TTTCAGACGGGCATTAACGATAGCGACATTGTGCATATCACCCTCGACCCACTCCGGTGAGGTGAt                                              <  1:269001/250‑1 (MQ=255)                                                                                                                                                                                                        gCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCATGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAAT‑ATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGG‑TTTCAGACGGGCATTAACGATATCGCCAGTGTGCATATCACCCGCGACCCACACCGGTGAGGTGATAAACCGCTCCAt                                  <  2:47717/251‑1 (MQ=255)                                                                                                                                                                                                         cGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAAT‑ATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGG‑TTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGGGAGGGGATAAACCGCTCCATc                                 >  1:131396/1‑251 (MQ=255)                                                                                                                                                                                                                        caaaaTCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAAT‑ATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGG‑TTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGc                  <  2:276589/249‑1 (MQ=255)                                                                                                                                                                                                                        caaaaTCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAAT‑ATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGG‑TTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGc                  <  1:70500/249‑1 (MQ=255)                                                                                                                                                                                                                                        ggCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAAT‑ATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGG‑TTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCt  <  1:335632/251‑1 (MQ=255)                                                                                                                                                                                                                                              gCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAAT‑ATCAATAGAAACCCACtt                                                                                                                                            <  2:374411/108‑1 (MQ=255)                                                                                                                                                                                                                                              gCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAAT‑ATCAATAGAAACCCACtt                                                                                                                                            >  1:374411/1‑108 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                              AAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCGGAAGACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACTGCGGGCGGCTGGCGACGTATTCCAGTTCGAAATCAAGCGAGGAGGCGTCGCCATTCTCCGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAAT‑ATCTATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGG‑TTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGACGCACATCGGCCT  >  NC_000913/64986‑65467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑