Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	65,208	A→G	L191L (CTT→CTC)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	65,208	0	A	G	100.0%	138.0 / NA	41	L191L (CTT→CTC)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/16);  total (25/16)

TTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCGGAAGACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACTGCGGGCGGCTGGCGACGTATTCCAGTTCGAAATCAAGCGAGGAGGCGTCGCCATTCTCCGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAAT‑ATCTATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGG‑TTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGC  >  NC_000913/64971‑65451                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                      tttAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGt                                                                                                                                                                                                                                          >  2:66568/1‑251 (MQ=255)    aGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCg                                                                                                                                                                                                                                        <  1:332365/250‑1 (MQ=255)     gCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTTCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGCTTTTCCAGTAGCTGCGGGCGGCTGGCGACTTATTCCATTTCAAAATCGAGCGCGGAGGCGTCGcc                                                                                                                                                                                                                                      >  2:14811/1‑251 (MQ=255)     gCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGcc                                                                                                                                                                                                                                      >  2:229206/1‑251 (MQ=255)       cTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCAt                                                                                                                                                                                                                                    >  2:335632/1‑251 (MQ=255)           gCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATtctc                                                                                                                                                                                                                                <  1:213415/251‑1 (MQ=255)            cAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATtctct                                                                                                                                                                                                                               <  1:221298/251‑2 (MQ=255)                aaaGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGcc                                                                                                                                                                                                                           >  2:301426/1‑251 (MQ=255)                   gACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGcccc                                                                                                                                                                                                                         >  1:336955/1‑250 (MQ=255)                                     gTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCt                                                                                                                                                                                                      <  1:301426/251‑1 (MQ=255)                                              ccACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGc                                                                                                                                                                                             >  1:22251/1‑251 (MQ=255)                                                   ccAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTc                                                                                                                                                                                         <  1:60264/250‑1 (MQ=255)                                                         cactgttatCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGATCGCGGAGGCGTCGCCATTCTCTGTTCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGcc                                                                                                                                                                                   <  1:353057/245‑1 (MQ=255)                                                                     gccccagccGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGCTGCAGTACAGc                                                                                                                                                                      >  2:77123/9‑251 (MQ=255)                                                                                         cGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACGGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCTACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCGGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTc                                                                                                                                                  <  1:301592/251‑1 (MQ=255)                                                                                                gCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGGGGCGGCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGGGGCGTATGGTTTCAat‑attc                                                                                                                                          >  1:74471/1‑249 (MQ=255)                                                                                                 cTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAg                                                                                                                                                                                      >  1:360164/1‑207 (MQ=255)                                                                                                 cTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAg                                                                                                                                                                                      <  2:360164/207‑1 (MQ=255)                                                                                                      cATGTTTTTGCAGCATTCGCAGATCGAACTGCACCCCGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGGGGTTTCACT‑ATCaataaa                                                                                                                                     >  2:51747/1‑248 (MQ=255)                                                                                                       aTGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGGGGGTTCAAT‑ATCAATAGaa                                                                                                                                    >  1:350839/1‑250 (MQ=255)                                                                                                           ttttGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAAT‑ATCAATAGAAAccc                                                                                                                                <  2:22251/250‑1 (MQ=255)                                                                                                              tGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGCTCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAAT‑ATAAATAGAAACCCACtt                                                                                                                            >  1:338818/1‑251 (MQ=255)                                                                                                                    aTTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGGTTCAAT‑CTCAATAGAAAACCACTTGCgcgg                                                                                                                      >  2:55624/1‑251 (MQ=255)                                                                                                                              cGAACTGCCCCACGTTAAAACCGCTGATCACATCAGAATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAGCTGCGGGCGGCTGGCGACGTATTCCCGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCAAGCATATAAACGATACGCTGCCCGCAGCCTTCCAGGCAGATGCAGTACAGCTCACCGTGGCGGGGGGTGTCAATTAT‑AAAAGCAACCCACTTGAACGGCGGagcat                                                                                                              >  2:16897/1‑245 (MQ=255)                                                                                                                              cGAACTGCACCACGTTCCAACCGATGATCACATCCGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCCAT‑ATCAATAGAAACCCACTTGAGCGGCGGACGAt                                                                                                              >  1:276589/1‑249 (MQ=255)                                                                                                                                                aaCCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAAT‑ATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGG‑TTTCAGa                                                                                         <  1:176540/251‑1 (MQ=255)                                                                                                                                                        atCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAAT‑ATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGG‑TTTCAGACGGGCAtt                                                                                 <  2:307096/251‑1 (MQ=255)                                                                                                                                                          cacaTCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGGTTCAAT‑ATCAATAGAAACCCACTTTAGCGGCGGGCGATAGTCGGGATGCGG‑GTTCAGACGGGCATTa                                                                                >  1:82540/1‑250 (MQ=255)                                                                                                                                                            caTCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACTGTGGCGGGTGGTTTTACT‑ATCACTAAAAAACCCCGTTAGCGGGGGGCGAGAGGCTGGATGCGG‑GTTTAGACAGGCATTAACg                                                                             >  2:285842/1‑251 (MQ=255)                                                                                                                                                                  gATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGGTTCAAT‑ATCAATAGAAACCCACCTGAGCGGCGGGCGATAGTCGGGAGGCGG‑GTTTAGAAGGGCATTAACGATAgcg                                                                       >  2:70500/1‑249 (MQ=255)                                                                                                                                                                      gTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGAGTCGCCATTCTCTGACCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCCGGCCGATGCAGTACAGCTCACCGTGGCGGGAGGGTTCAAC‑ATCCATAGAAAACCCCTTGACCGGCTGAAGATAGTATGGATACGG‑GTTAAGACGGGGAGTAACGATTGCGCCAt                                                                   >  1:353067/1‑251 (MQ=255)                                                                                                                                                                              cAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGGTTCAAT‑ATCAATAGAAACCCACTTGAGCGGCGGGCGGTAGGTGGGGTGCGG‑GTTTAGACGGGCAATAAAGATAggccccttgtggat                                                            >  2:11701/1‑237 (MQ=255)                                                                                                                                                                               aaaCCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGGGGGTTTAAT‑ATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGG‑TTTCAGACGGGCATTAACGATAGCGCCATTGTGCatat                                                          >  1:297806/1‑251 (MQ=255)                                                                                                                                                                                      gCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAAT‑ATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGG‑TTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTc                                                   <  1:11419/251‑1 (MQ=255)                                                                                                                                                                                          tGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGGTTCAAT‑ATCAATAGAAAACCACTTGAGCGGCGGGCGGTAGGCCGGCTGCGGGTTTC‑GACGGGCGTTAACGAAAGCGCCCTTGTTCATATCAccccccacc                                               >  2:282413/1‑245 (MQ=255)                                                                                                                                                                                           gAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGGTTCCAT‑ATCAATAGAAACCCACTTGAGAGGCGGGCGGTAGGCGGGGTGCGG‑GTTTAAACGGGCATTTAAGATAGAGCCATTGGTCATATCACCCTCGAccc                                              >  2:126449/1‑251 (MQ=255)                                                                                                                                                                                                            gcgggcggCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAAT‑ATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGG‑TTTCAGACGGGCATTAACGATAGCGACATTGTGCATATCACCCTCGACCCACTCCGGTGAGGTGAt                              <  1:269001/250‑1 (MQ=255)                                                                                                                                                                                                                       gCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCATGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAAT‑ATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGG‑TTTCAGACGGGCATTAACGATATCGCCAGTGTGCATATCACCCGCGACCCACACCGGTGAGGTGATAAACCGCTCCAt                  <  2:47717/251‑1 (MQ=255)                                                                                                                                                                                                                        cGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAAT‑ATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGG‑TTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGGGAGGGGATAAACCGCTCCATc                 >  1:131396/1‑251 (MQ=255)                                                                                                                                                                                                                                       caaaaTCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAAT‑ATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGG‑TTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGc  <  2:276589/249‑1 (MQ=255)                                                                                                                                                                                                                                       caaaaTCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAAT‑ATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGG‑TTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGc  <  1:70500/249‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                      TTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCGGAAGACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACTGCGGGCGGCTGGCGACGTATTCCAGTTCGAAATCAAGCGAGGAGGCGTCGCCATTCTCCGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAAT‑ATCTATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGG‑TTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGC  >  NC_000913/64971‑65451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑