Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 86,345 G→C C239S (TGC→TCC)  ilvI → acetolactate synthase/acetohydroxybutanoate synthase, catalytic subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091386,3450GC93.8% 81.6 / ‑4.9 32C239S (TGC→TCC) ilvIacetolactate synthase/acetohydroxybutanoate synthase, catalytic subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (20/10);  minor base T (2/0);  total (22/10)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCGGTTGTCTACGTAGGCGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGCATATCGATATTGATCCTACTTCCATTTCTAAAACCGTGACTGCGGATATCCCGATTG  >  NC_000913/86102‑86590
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     
tCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGGGGGGCAATCACGGCGGGGTGACATCCGCAGTTGAAAGAAACGGGGGGGGGGGTGGAACTGCCCGTTGTTTCCTCAttt                                                                                                                                                                                                                                                >  2:263503/1‑250 (MQ=255)
                      tCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCg                                                                                                                                                                                                                           <  2:329330/250‑1 (MQ=255)
                        ttAATCCGGCGAACAAATTACCCTATGTCTGGCAGGAGTCGGTCAGTATGCGTTCTTACAATCCCCCTAATACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATAACGGCGGACTGCAATCAGCAGTTGAAAGAAATGGTGGAGGCGTTTAATATGCCCGTTGGTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGc                                                                                                                                                                                                                         >  1:363308/1‑250 (MQ=255)
                         tAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGTTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCgg                                                                                                                                                                                                                          <  2:361254/248‑1 (MQ=255)
                            tCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCCTCAACAGTTGAAAGAAATGGGGGAGGGGGTGAATCTGCCCGTTGTTTCCTCCTTGATGGGGCTGGGGGCGttttcgcccacgg                                                                                                                                                                                                                    >  1:357163/1‑241 (MQ=255)
                            tCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGGGTTGAATCTGGCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGGTTCCGGCAACGc                                                                                                                                                                                                                    >  2:160985/1‑251 (MQ=255)
                                 cGAACAAATTAACCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGGGGAGGCGTTGAATCTTCCCGTTGTTTCCTCATTTATGGGGCTGGGGGCGTTTCCGGCAACGCATCgg                                                                                                                                                                                                               >  2:316927/1‑250 (MQ=255)
                                                     tGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAAAGCATCGTCAGGCACTGGGCATGCTggg                                                                                                                                                                                           >  1:372367/1‑251 (MQ=255)
                                                       gCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGGGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGa                                                                                                                                                                                          >  1:276712/1‑250 (MQ=255)
                                                           gAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGc                                                                                                                                                                                      >  1:39454/1‑250 (MQ=255)
                                                                 gTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATACAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCCTCAGCAGTTGAAAGAAATTGGGGGGGGGTTGAATCTGCCCGTTGTTTTCTCTTTGATGGGGCTGGGGGGGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCAAGGTAc                                                                                                                                                                               >  2:115751/1‑251 (MQ=255)
                                                                   cAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTAcc                                                                                                                                                                              <  1:8882/250‑1 (MQ=255)
                                                                      tATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCAGTCCTCAGCAGTTTAAAGAAATGGGGGAGGCGTTGGATATGCCCGTTGTTTCCTAATTGATGGGGATGGGGGCGTTTTCGGCAACGCATCGGCAGGCACTGGGCATGGTGGGAATGCACGGGACCTACg                                                                                                                                                                          >  2:25718/1‑251 (MQ=255)
                                                                                         actactACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCTGCAACGCAGCGTCAGGCACTGGGGATGCTGGGAATGCTCGGTACCTACGAAGCCAATATGACGATGCa                                                                                                                                                       <  1:33696/251‑1 (MQ=255)
                                                                                                           gggCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCCGCCATCAGCAACTGAAAGAAATGGGGGCGGCGTTGAATCTGACCGTTGTTTCCTCATTCATGGGGCCGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGGATGCACGGTACCTACGAAGCCCATATGACGATGCATAACGCGGATTTGAtttt                                                                                                                                     >  2:179753/1‑251 (MQ=255)
                                                                                                                       cGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTCCGTAGGTGGTGGGGCAATCACGGCGGGCCGCCATCAGCAAGTGAAAGAAATGGGGGCGGCGTTGAATCTGCCCGCTGTTTTCTCATTGGTGGGGCTGGGGGCGTTTCCGGCCACGCATCGTCAGGCAATGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAAAGCGGATGTGATTTTCGCCGTCGGGGt                                                                                                                         >  1:230474/1‑251 (MQ=255)
                                                                                                                         tGCTCTGCAAAAGCTGGTGGCGGCAAAACAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGGTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGACAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCgggggac                                                                                                                       >  1:83381/1‑248 (MQ=255)
                                                                                                                               gCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGAtt                                                                                                                   <  1:309866/249‑1 (MQ=255)
                                                                                                                                                                       ggtgggggggCAATGACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAAGCTGCCCGTTGTGTCCCCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGATGGTAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGGGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCaaa                                                                         <  2:304149/245‑1 (MQ=255)
                                                                                                                                                                                   aTCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCt                                                             <  2:372367/251‑1 (MQ=255)
                                                                                                                                                                                      aCGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGGACGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGcc                                                          >  2:104666/1‑250 (MQ=255)
                                                                                                                                                                                                gCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGCATATCGATAtt                                                >  2:153620/1‑251 (MQ=255)
                                                                                                                                                                                                       gcagTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGCATATCGATATTGATCCt                                          >  1:151675/1‑250 (MQ=255)
                                                                                                                                                                                                                 gaaaTGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGTCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGCATATCGATATTGATCCTACTTCCATTTc                               >  1:330133/1‑251 (MQ=255)
                                                                                                                                                                                                                    atggTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGCATATCGATATTGATCCTACTTCCATTTCTa                             >  1:161028/3‑250 (MQ=255)
                                                                                                                                                                                                                       gTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGCATATCGATATTGATCCTACTTCCAt                                  <  2:242661/242‑1 (MQ=255)
                                                                                                                                                                                                                       gTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGCATATCGATATTGATCCTACTTCCAt                                  >  1:242661/1‑242 (MQ=255)
                                                                                                                                                                                                                               gTTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGa                                                                                                                                                                         <  1:49410/99‑1 (MQ=255)
                                                                                                                                                                                                                               gTTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGa                                                                                                                                                                         >  2:49410/1‑99 (MQ=255)
                                                                                                                                                                                                                                 tGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGACGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGCATATCGATATTGATCCTACTTCCATTTCTAAAACCGTGACTGCg               >  2:374035/1‑251 (MQ=255)
                                                                                                                                                                                                                                         cccGTTTTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACTGTACCTACGAAGCCAATATTACGATTCATTACGCGTATGTGATTTTCGCCGTCGGGGTACGATTTGATGTCCGAACGACGAACAATCTGGCAAAGTACTGCCTAAATGCCACTGTTCTTCATATCGATATTGAGCCTACTTCCATTTCTAAACCCGttctttcggatatcc        <  2:363308/250‑15 (MQ=255)
                                                                                                                                                                                                                                              tgttTCCTAATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGCATATCGATATTGATCCTACTTCCATTTCTAAAACCGTGACTGCGGATATCCCGATtg  >  2:89988/1‑251 (MQ=255)
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     
TCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCGGTTGTCTACGTAGGCGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGCATATCGATATTGATCCTACTTCCATTTCTAAAACCGTGACTGCGGATATCCCGATTG  >  NC_000913/86102‑86590

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: