Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	86,241	A→G	V204V (GTA→GTG)	ilvI →	acetolactate synthase/acetohydroxybutanoate synthase, catalytic subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	86,241	0	A	G	100.0%	115.1 / NA	35	V204V (GTA→GTG)	ilvI	acetolactate synthase/acetohydroxybutanoate synthase, catalytic subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/14);  total (21/14)

CCGGTGGTTAAACACAGTTTTCTGGTTAAGCAAACGGAAGACATTCCGCAGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCGGTTGTCTACGTAGGCGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATT  >  NC_000913/85999‑86477                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                             ccGGTGGTTAAACACAGTTTTCTGGTTAAGCAAACGGAAGACATTCCGCAGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCaa                                                                                                                                                                                                                                       >  2:15745/1‑250 (MQ=255)         tAAACACAGTTTTCTGGTTAAGCAAACGGAAGACATTCCGCAGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCg                                                                                                                                                                                                                               <  1:15745/250‑1 (MQ=255)                      cTGGTTAAGCAAACGGAAGACATTCCGCAGGTGCTGCAATAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTACGCGTGCTCTGCAAACGCTGGTGGCGGCAAAATAACCGGTTGTCTACGTAgg                                                                                                                                                                                                                 >  2:25514/1‑251 (MQ=255)                        ggTTAAGCAAACGGAAGACATTCCGCAGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAggtg                                                                                                                                                                                                               <  1:62872/251‑3 (MQ=255)                           tAAGCAAACGGAAGACATTCCGCAGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAACAAAACCGGTTGTCTACGTAggtgg                                                                                                                                                                                                              >  2:257678/1‑246 (MQ=255)                              gCAAACGGAAGACATTCCGCAGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTgggg                                                                                                                                                                                                         <  2:51574/251‑1 (MQ=255)                                   cGGAAGACATTCCGCAGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATc                                                                                                                                                                                                    <  1:77563/251‑1 (MQ=255)                                    ggAAGACATTCCGCAGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGTTGGTGGGGCAATCa                                                                                                                                                                                                   <  1:297880/251‑1 (MQ=255)                                     gAAGACATTCCGCCGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCTGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCCAAAAAACCGGTTGTCTACGTAGGGGGTGGGGCAATCa                                                                                                                                                                                                   >  2:257383/1‑250 (MQ=255)                                                gcaagagCTGCAAAACGCTTTATGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGCATTACGGAAAGATACTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGCAGGTCAGTATGAGATCTTACAATCCAACAACTACGGGACATACAGGGCAAATTAAGCGTGCTCTGCAAACACTGGTGGCGGAAAAAAAACAGGTTGTCTACGTAGGCGGCAGGGCAACAACGGCggggagcc                                                                                                                                                                                       >  2:349673/7‑246 (MQ=255)                                                      gCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCCCGGCGGGTTGCCCTcagc                                                                                                                                                                                 >  2:290588/1‑251 (MQ=255)                                                         gAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCGGCAGt                                                                                                                                                                              <  1:11065/251‑1 (MQ=255)                                                                       ggctggcGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTgaaagaaatgg                                                                                                                                                                  <  1:356699/249‑4 (MQ=255)                                                                                   gTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTTGAGGCGTTGAAt                                                                                                                                                    >  1:253021/1‑251 (MQ=255)                                                                                                  cAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGtttcc                                                                                                                                     <  1:365076/251‑3 (MQ=255)                                                                                                        tCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGGGGGGCAATCACGGCGGGGTGACATCCGCAGTTGAAAGAAACGGGGGGGGGGGTGGAACTGCCCGTTGTTTCCTCAttt                                                                                                                               >  2:263503/1‑250 (MQ=255)                                                                                                                              tCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCg                                                                                                          <  2:329330/250‑1 (MQ=255)                                                                                                                                ttAATCCGGCGAACAAATTACCCTATGTCTGGCAGGAGTCGGTCAGTATGCGTTCTTACAATCCCCCTAATACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATAACGGCGGACTGCAATCAGCAGTTGAAAGAAATGGTGGAGGCGTTTAATATGCCCGTTGGTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGc                                                                                                        >  1:363308/1‑250 (MQ=255)                                                                                                                                 tAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGTTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCgg                                                                                                         <  2:361254/248‑1 (MQ=255)                                                                                                                                 tAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAATAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCt                                                                                                                                                                                          >  2:259937/1‑167 (MQ=255)                                                                                                                                 tAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCt                                                                                                                                                                                          <  1:259937/167‑1 (MQ=255)                                                                                                                                    tCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCCTCAACAGTTGAAAGAAATGGGGGAGGGGGTGAATCTGCCCGTTGTTTCCTCCTTGATGGGGCTGGGGGCGttttcgcccacgg                                                                                                   >  1:357163/1‑241 (MQ=255)                                                                                                                                    tCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGGGTTGAATCTGGCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGGTTCCGGCAACGc                                                                                                   >  2:160985/1‑251 (MQ=255)                                                                                                                                         cGAACAAATTAACCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGGGGAGGCGTTGAATCTTCCCGTTGTTTCCTCATTTATGGGGCTGGGGGCGTTTCCGGCAACGCATCgg                                                                                              >  2:316927/1‑250 (MQ=255)                                                                                                                                                             tGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAAAGCATCGTCAGGCACTGGGCATGCTggg                                                                          >  1:372367/1‑251 (MQ=255)                                                                                                                                                               gCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGGGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGa                                                                         >  1:276712/1‑250 (MQ=255)                                                                                                                                                                   gAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGc                                                                     >  1:39454/1‑250 (MQ=255)                                                                                                                                                                         gTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATACAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCCTCAGCAGTTGAAAGAAATTGGGGGGGGGTTGAATCTGCCCGTTGTTTTCTCTTTGATGGGGCTGGGGGGGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCAAGGTAc                                                              >  2:115751/1‑251 (MQ=255)                                                                                                                                                                           cAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTAcc                                                             <  1:8882/250‑1 (MQ=255)                                                                                                                                                                              tATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCAGTCCTCAGCAGTTTAAAGAAATGGGGGAGGCGTTGGATATGCCCGTTGTTTCCTAATTGATGGGGATGGGGGCGTTTTCGGCAACGCATCGGCAGGCACTGGGCATGGTGGGAATGCACGGGACCTACg                                                         >  2:25718/1‑251 (MQ=255)                                                                                                                                                                                                 actactACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCTGCAACGCAGCGTCAGGCACTGGGGATGCTGGGAATGCTCGGTACCTACGAAGCCAATATGACGATGCa                                      <  1:33696/251‑1 (MQ=255)                                                                                                                                                                                                                   gggCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCCGCCATCAGCAACTGAAAGAAATGGGGGCGGCGTTGAATCTGACCGTTGTTTCCTCATTCATGGGGCCGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGGATGCACGGTACCTACGAAGCCCATATGACGATGCATAACGCGGATTTGAtttt                    >  2:179753/1‑251 (MQ=255)                                                                                                                                                                                                                               cGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTCCGTAGGTGGTGGGGCAATCACGGCGGGCCGCCATCAGCAAGTGAAAGAAATGGGGGCGGCGTTGAATCTGCCCGCTGTTTTCTCATTGGTGGGGCTGGGGGCGTTTCCGGCCACGCATCGTCAGGCAATGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAAAGCGGATGTGATTTTCGCCGTCGGGGt        >  1:230474/1‑251 (MQ=255)                                                                                                                                                                                                                                 tGCTCTGCAAAAGCTGGTGGCGGCAAAACAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGGTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGACAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCgggggac      >  1:83381/1‑248 (MQ=255)                                                                                                                                                                                                                                       gCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGAtt  <  1:309866/249‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                             CCGGTGGTTAAACACAGTTTTCTGGTTAAGCAAACGGAAGACATTCCGCAGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCGGTTGTCTACGTAGGCGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATT  >  NC_000913/85999‑86477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑