Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	85,481	T→C	R11G (AGA→GGA)	yabR ←	protein YabR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	85,481	0	T	C	100.0%	78.3 / NA	24	R11G (AGA→GGA)	yabR	protein YabR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/12);  total (12/12)

GCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAAAAATAGAGAAATTGCTGTAAGTTGTGGGATTCAGCCGATTTATTATCAATTTAATCCTCTGTAATGGAGGATTTTATCGTTTCTTTTCACCTTTCCTCCTGTTTATTCTTATTACCCCGTGTTTATGTCTCTGGCTGCCAATTGCTTAAGCAAGATCGGACGGTTAATGTGTTTTACACATTTTTTCCGTCAAACAGTGAGGCAGGCCATGGAGATGTTGTCTGGAGCCGAGATGGTCGTCCGATCGCTTATCGATCAGGGCGTTAAACAAGTATTCGGTTATCCCGGAGGCGCAGTCCTTGAT  >  NC_000913/85250‑85725                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     gCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAAAAATAGAGAAATTGCTGTAAGTTGTGGGATTCAGCCGATTTATTATCAATTTAATCCTCCGTAATGGAGGATTTTAt                                                                                                                                                                                                                                     <  1:264194/249‑1 (MQ=255)                ggCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAAAAATAGAGAAATTGCTGTAAGTTGTGGGATTCAGCCGATTTATTATCAATTTAATCCTCCGTAATGGAGGATTTTATCGTTTCTTTTCACCttt                                                                                                                                                                                                                    <  1:347601/251‑1 (MQ=255)                      cAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAAAAATAGAGAAATTGCTGTAAGTTGTGGGATTCAGCCGATTTATTATCAATTTAATCCTCCGTAATGGAGGATTTTATCGTTTCTTTTCACCTTTcctcct                                                                                                                                                                                                              >  2:242209/1‑251 (MQ=255)                        gTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAAAAATAGAGAAATTGCTGTAAGTTGTGGGATTCAGCCGATTTATTATCAATTTAATCCTCCGTAATGGAGGATTTTATCGTTTCTTTTCACCTTTCCTCCTGt                                                                                                                                                                                                            >  2:95134/1‑251 (MQ=255)                                                aaTTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTGTAGGATGAATTAAAAAAATAGAGAAATTGCTGTAAGTTGTGGGATTCAGCCGATTTATTATCAATTTAATCCTCCGTAATGGAGGATTTTATCGTTTCTTTTCACCTTTCCGGCTGTTTATTCGTATGACCTCGTGTTTAt                                                                                                                                                                                    <  1:255543/251‑1 (MQ=255)                                                                       cAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAAAAATAGAGAAATTGCTGTAAGTTGTGGGATTCAGCCGATTTATTATCAATTTAATCCTCCGTAATGGAGGATTTTATCGTTTCTTTTCACCTTTCCTCCTGTTTATTCTTATTACCCCGTGTTTATGTCTCTGGCTGCCAATTGCTTa                                                                                                                                                              >  2:41269/1‑250 (MQ=255)                                                                                                                  tATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAAAAATAGAGAAATTGCTGTAAGTTGTGGGATTCAGCCGATTTATTATCAATTTAATCCTCCGTAATGGAGGATTTTATCGTTTCTTTTCACCTTTCCTCCTGTTTATTCTTATTACCCCGTGTTTATGTCTCTGGCTGCCAATTGCTTAAGCAAGATCGGCCGGTTAATGTGTTTTACACATTTTTTCCGTc                                                                                                                   >  1:23665/1‑250 (MQ=255)                                                                                                                       actGTACAATTTTATTCCAACTGTGTGGGCTCCCTATTTTAGGATTAATTAAACAAATAGACAAATAGCTGTACGTTGTGGGCTTAAGCCGATTTATTATCACTTTAATCCTCCGTAATGGAGGATTTTATCGTTTCTTTTCACATTTCCTCCTGTTTATTCTTATTAACCCGTGTTTATGTATCTGGCTGCCAATTGCTTAAGCAAGATCGGCAGGTTAATGTGTTTTACACATTTTTTCCGTCAAAc                                                                                                               >  2:349713/3‑249 (MQ=255)                                                                                                                                         gAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAAAAATAGAGAAATTGCTGTAAGTTGTGGGATTCAGACGATTTATTATCAATTTAATCCTCCGTAATGGAGGATGTTATCGTTTCTTTTCACCTTTCCTCCTGTTTATTCTTATTACCCCGTGTTTATGTCTCTGGCTGCCAATTGCTTAAGCAAGATCGGACGGTTAATGTGTTTTACACATTTTTTCCGTCAAACAGTGAGGCAGGCCATGgaga                                                                                           <  1:343489/251‑1 (MQ=255)                                                                                                                                                             ttAGGATTAATTAAAAAAATAGAGAAATTGCTGTAAGTTGTGGGATTCAGCCGATTTATTATCAATTTAATCCTCCGTAATGGAGGATTTTATCGTTTCTTTTCACCTTTCCTCCTGTTTATTCTTATTACCCCGTGTTTATGTCTCTGGCTGCCAATTGCTTAAGCAAGATCGGACGGTTAATGTGTTTTACACATTTTTTCCGTCAAACAGTGAGGCAGGCCATGGAGATGTTGTCTGGAGCCGAGATg                                                                       >  1:125216/1‑251 (MQ=255)                                                                                                                                                                         aaaaaaaTAGAGAAATTGCTGTAAGTTGTGGGATTCAGCCGATTTATTATCAATTTAATCCTCCGTAATGGAGGATTTTATCGTTTCTTTTCACCTTTCCTCCTGTTTATTCTTATTACCCCGTGTTTATGTCTCTGGCTGCCAATTGCTTAAGCAAGATCGGACGGTTAATGTGTTTTACACATTTTTTCCGTCAAACAGTGAGGCAGGCCa                                                                                                 >  1:170855/1‑213 (MQ=255)                                                                                                                                                                         aaaaaaaTAGAGAAATTGCTGTAAGTTGTGGGATTCAGCCGATTTATTATCAATTTAATCCTCCGTAATGGAGGATTTTATCGTTTCTTTTCACCTTTCCTCCTGTTTATTCTTATTACCCCGTGTTTATGTCTCTGGCTGCCAATTGCTTAAGCAAGATCGGACGGTTAATGTGTTTTACACATTTTTTCCGTCAAACAGTGAGGCAGGCCa                                                                                                 <  2:170855/213‑1 (MQ=255)                                                                                                                                                                         aaaaaaaTAGAGAAATTGCTGTAAGTTGTGGGATTCAGCCGATTTATTATCAATTTAATCCTCCGTAATGGAGGATTTTATCGTTTCTTTTCACCTTTCCTCCTGTTTATTCTTATTACCCCGTGTTTATGTCTCTGGCTGCCAATTGCTTAAGCAAGATCGGACGGTTAATGTGTTTTACACATTTTTTCCGTCAAACAGTGAGGCAGGCCATGGAGATGTTGTCTGGAGCCGAGATGGTCGTCCGATc                                                            <  1:281087/250‑1 (MQ=255)                                                                                                                                                                            aaaaTAGAGAAATTGCTGTAAGTTGTGGGATTCAGCCGATTTATTATCAATTTAATCCTCCGTAATGGAGGATTTTATCGTTTCTTTTCACCTTTCCTCCTGTTTATTCTTATTACCCCGTGTTTATGTCTCTGGCTGCCAATTGCTTAAGCAAGATCGGACGGTTAATGTGTTTTACACATTTTTTCCGTCAAACAGTGAGGCAGGCCATGGAGATGTTGTCTGGAGCCGAGATGGTCGTCCGATCGCtt                                                        <  1:99719/251‑1 (MQ=255)                                                                                                                                                                                      aaTTGCTGTAAGTTGTGGGATTCAGCCGATTTATTATCAATTTAATCCTCCGTAATGGAGGATTTTATCGTTTCTTTTCACCTTTCCTCCTGTTTATTCTTATTACCCCGTGTTTATGTCTCTGGCTGCCAATTGCTTAAGCAAGATCGGACGGTTAATGTGTTTTACACATTTTTTCCGTCAAACAGTGAGGCAGGCCATGGAGATGTTGTCTGGAGCCGAGATGGTCGTCCGATCGCTTATCGATCAgg                                              <  1:115575/251‑1 (MQ=255)                                                                                                                                                                                      aaTTGCTGTAAGTTGTGGGATTCAGCCGATTTATTATCAATTTAATCCTCCGTAATGGAGGATTTTATCGTTTCTTTTCACCTTTCCTCCTGTTTATTCTTATTACCCCGTGTTTATGTCTCTGGCTGCCAATTGCTTAAGCAAGATCGGACGGTTAATGTGTTTTACACATTTTTTCCGTCAAACAGTGAGGCAGGCCATGGAGATGTTGTCTGGAGCCGAGATGGTCGTCCGATCGCTTATCGATCAgg                                              >  2:93739/1‑251 (MQ=255)                                                                                                                                                                                      aaTTGCTGTAAGTTGTGGGATTCAGCCGATTTATTATCAATTTAATCCTCCGTAATGGAGGATTTTATCGTTTCTTTTCACCTTTCCTCCTGTTTATTCTTATTACCCCGTGTTTATGTCTCTGGCTGCCAATTGCTTAAGCAAGATCGGACGGTTAATGTGTTTTACACATTTTTTCCGTCAAACAGTGAGGCAGGCCATGGAGATGTTGTCTGGAGCCGAGATGGTCGTCCGATCGCTTATCGATCAgg                                              >  1:223158/1‑251 (MQ=255)                                                                                                                                                                                         tGCTGTAAGTTGTGGGATTCAGCCGATTTATTATCAATTTAATCCTCCGTAATGGAGGATTTTATCGTTTCTTTTCACCTTTCCTCCTGTTTATTCTTATTACCCCGTGTTTATGTCTCTGGCTGCCAATTGCTTAAGCAAGATCGGACGGTTAATGTGTTTTACACATTTTTTCCGTCAAACAGTGAGGCAGGCCATGGAGATGTTGTCTGGAGCCGAGATGGTCGTCCGATCGCTTATCGATCAGGGc                                            <  1:64391/250‑1 (MQ=255)                                                                                                                                                                                            tGTAAGTTGTGGGATTCAGCCGATTTATTATCAATTTAATCCTCCGTAATGGAGGATTTTATCGTTTCTTTACACCTTTCCTCCTGTTTATTCTTATTACCCCGTGTTTATGTCTCTGGCTGCCAATTGCTTAAGCAAGa                                                                                                                                                       >  2:196929/1‑140 (MQ=255)                                                                                                                                                                                            tGTAAGTTGTGGGATTCAGCCGATTTATTATCAATTTAATCCTCCGTAATGGAGGATTTTATCGTTTCTTTACACCTTTCCTCCTGTTTATTCTTATTACCCCGTGTTTATGTCTCTGGCTGCCAATTGCTTAAGCAAGa                                                                                                                                                       <  1:196929/140‑1 (MQ=255)                                                                                                                                                                                                          ttCAGCCGATTTATTATCAATTTAATCCTCCGTAATGGAGGATTTTATCGTTTCTTTTCACCTTTCCTCCTGTTTATTCTTATTACACCGTGTTTATGTCTCTGGCTGCCAATTGCTTAAGCAAGATCGGACGGTTAATGTGTTTTACACATTTTTTCCGTCAAACAGTGAGGCAGGCCATGGAGATGTTGTCTGGAGCCGAGATGGTCGTACGATCGCTTATCGATAAGGCCGTTAAAAAAGTATTCGGt                          >  2:297880/1‑251 (MQ=255)                                                                                                                                                                                                                    ttattaTCAATTTAATCCTCCGTAATGGAGGATTTTATCGTTTCTTTTCACCTTTCCTCCTGTTTATTCTTATTACCCCGTGTTTATGTCTCTGGCTGCCAATTGCTTAAGCAAGATCGGACGGTTAATGTGTTTTACACATTTTTTCCGTCAAACAGTGAGGCAGGCCATGGAGATGTTGTCTGGAGCCGAGATGGTCGTCCGATCGCTTATCGATCAGGGCGTTAAACAAGTATTCGGTTATCCCgg                  >  1:309869/1‑249 (MQ=255)                                                                                                                                                                                                                                tAATCCTCCGTAATGGAGGATTTTATCGTTTCTTTTCTCCTTTCCTCCTGTTTATTCTTATTACCCCGTGTTTATGTCTCTGGCTTCCAATTGCTTAAGCAAGATCGGACGGTTAATTTGTGTTACACATTTTTTCCGTCAAACAGTGAGGCATGCCATGGAGATGTTGTCGGGAGCCGAGATGGTCGTCCTATCGCTTATCGATCAGGGCGTTAAACAAGTATTCGGTTATCCCGGTGTCGCAGTCCtt     <  2:222382/250‑1 (MQ=255)                                                                                                                                                                                                                                  aTCCTCCGTAATGGAGGATTTTATCGTTTCTTTTCACCTTTCCTCCTGTTTATTCTTATTACCCCGTGTTTATGTCTCTGGCTGCCAATTGCTTAAGCAAGATCGGACGGTTAATGTGTTTTACACATTTTTTCCGTCAAACAGTGAGGCAGGCCATGGAGATGTTGTCTGGAGCCGAGATGGTCGTCCGATCGCTTATCGATCAGGGCGTTAAACAAGTATTCGGTTATCCCGGAGGCGCAGTCCTTGat  <  1:13560/251‑1 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     GCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAAAAATAGAGAAATTGCTGTAAGTTGTGGGATTCAGCCGATTTATTATCAATTTAATCCTCTGTAATGGAGGATTTTATCGTTTCTTTTCACCTTTCCTCCTGTTTATTCTTATTACCCCGTGTTTATGTCTCTGGCTGCCAATTGCTTAAGCAAGATCGGACGGTTAATGTGTTTTACACATTTTTTCCGTCAAACAGTGAGGCAGGCCATGGAGATGTTGTCTGGAGCCGAGATGGTCGTCCGATCGCTTATCGATCAGGGCGTTAAACAAGTATTCGGTTATCCCGGAGGCGCAGTCCTTGAT  >  NC_000913/85250‑85725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑