Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	79,585	G→A	T427I (ACC→ATC)	leuC ←	3‑isopropylmalate dehydratase subunit LeuC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	79,585	0	G	A	100.0%	154.3 / NA	44	T427I (ACC→ATC)	leuC	3‑isopropylmalate dehydratase subunit LeuC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/23);  total (21/23)

CCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCGACATTGGCGGCATCCAGCGGAACCACCAGGCCTGTGTGTTTGATAAATTTCTCTGCCATGGTGTGCTCCTTATTTAATGTTGCGAATGTCGGCGAAATGTCCGGTCACAGCAGCAGCGGCAGCCATTGCCGGGCTGACCAGATGCGTGCGCCCGCCGCGCCCCTGGCGGCCTTCAAAGTTACGGTTGCTGGTGGAGGCACAACGTTCGCCCGGATTCAGACGGTCGTTGTTCATCGCCAGACACATTGAGCAGCCAGGCAAGCGCCATTCAAAACCGGCTTCAATAAAGATTTTATCCAGACCTTCCGCTTCCGCCTGGGCTTTTACCGGGCCAGAGCCGGGAACCACCAGTGCCTGCACGCCTGGCGCGACTTTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGA  >  NC_000913/79342‑79829                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     ccGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCGACATTGGCGGCATCCAGCGGAACCACCAGGCCTGTGTGTTTGATAAATTTCTCTGCCATGGTGTGCTCCTTATTTAATGTTGCGAATGTCGGCGAAATGTCCGGTCACAGCAGCAGCGGCAGCCATTGCCGGGCTGACCAGATGCGTGCGCCCGCCGCGCCCCTGGCGGCCTTCAAAGTTACGGTTGCTGATGGAGGc                                                                                                                                                                                                                                               >  1:178884/1‑251 (MQ=255)         gTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCGACATTGGCGGCATCCAGCGGAACCACCAGGCCTGTGTGTTTGATAAATTTCTCTGCCATGGTGTGCTCCTTATTTAATGTTGCGAATGTCGGCGAAATGTCCGGTCACAGCAGCAGCGGCAGCCATTGCCGGGCTGACCAGATGCGTGCGCCCGCCGCGCCCCTGGCGGCCTTCAAAGTTACGGTTGCTGATGGAGGCACAACGtt                                                                                                                                                                                                                                       >  1:7971/1‑251 (MQ=255)                             tttCGGGATGATTGCATCGGTATCGACATTGGCGGCATCCAGCGGAACCACCAGGCCTGTGTGTTTGATAAATTTCTCTGCCATGGTGTGCTCCTTATTTAATGTTGCGAATGTCGGCGAAATGTCCGGTCACAGCAGCAGCGGCAGCCATTGCCGGGCTGACCAGATGCGTGCGCCCGCCGCGCCCCTGGCGGCCTTCAAAGTTACGGTTGCTGATGGAGGCACAACGTTCGCCCGGATTCAGACGGTCg                                                                                                                                                                                                                   >  1:240145/1‑251 (MQ=255)                                      gatTGCATCGGTATCGACATTGGCGGCATCCAGCGGAACCACCAGGCCTGTGTGTTTGATAAATTTCTCTGCCATGGTGTGCTCCTTATTTAATGTTGCGAATGTCGGCGAAATGTCCGGTCACAGCAGCAGCGGCAGCCATTGCCGGGCTGACCAGATGCGTGCGCCCGCCGCGCCCCTGGCGGCCTTCAAAGTTACGGTTGCTGATGGAGGCACAACGTTCGCCCGGATTCAGACGGTCGTTGTTCATc                                                                                                                                                                                                          <  1:212942/251‑1 (MQ=255)                                      gagtGCATCGGTATCGACATGGGCGGCTTCGAGCGGAACCCGCAGGCCTGTGTGTTTGATAAATTTCTCGGCAAGGGTTGGCTCTTTATTTAATGTTGCGATGGACGGCGTAATGTCTGGTCCTAGCACCGGCGCCCGCCATGGCCGGGGTTAGCAGGTGCGTGCGTCCGGCGCGCCCCTGGCTGCTTTCAAAGTTACGGTTGCTGATGGAGGGACAACGTTCGCCCGGATTCAGACGGTCGTTGTTCATc                                                                                                                                                                                                          <  1:178536/248‑1 (MQ=255)                                            aTCGGTATCGACATTGGGGGCTTCCTGCGGAACCCCCAGGCCTGTGTGTTTGATAAATTTCTCGGCCATGGTGTGCTCCTTATTTAATGTTGCGAATGTCGGCGAAATGTCCGGTCACAGCAGCAGCGGCTGCCCTTGCCGGTCTGTCCAGTTGCGTGCGCCCGCCGCGCCCCTGGCGTGCTTCAAAGTTACGGTTGCTTATGGAGGCACAACGTTCGCCCGGATTCAGACGGTCTTTGTTCATCGCCAGa                                                                                                                                                                                                    <  1:187513/251‑1 (MQ=255)                                              cggtagcgcaatgggtggcATCCAGCGGAACCACCAGGCCTGTGTGTTTTATAAATTTCTCTGCCATGGTGTGCTCTTTATTTAATGTTGCGAATGTCGGCGAAATGTCCGGTCACAGCGGCAGCGGCGGCCTTTGCCGGGCTGTCCAGATGCGTGCGCCCGCCGCGCCCCTGGCGGCCTTCAAAGTTACGGTTGCTGATGGAGGCACAACGTTCGCCCGGATTCAGACGGTCGTTGTTCATCGCCAGaca                                                                                                                                                                                                  <  1:250352/235‑1 (MQ=255)                                              cGGTATCGACATTGGCGGCATCCAGCGGAACCACCAGGCCTGTGTGTTTGATAAATTTCTCTGCCATGGTGTGCTCCTTATTTAATGTTGCGAATGTCGGCGAAATGTCCGGTCACAGCAGCAGCGGCAGCCATTGCCGGGCTGACCAGATGCGTGCGCCCGCCGCGCCCCTGGCGGCCTTCAAAGTTACGGTTGCTGATGGAGGCACAACGTTCGCCCGGATTCAGACGGTCGTTGTTCATCGCCAGac                                                                                                                                                                                                   <  1:274864/250‑1 (MQ=255)                                                 tATCGACATTGGCGGCATCCAGCGGAACCACCAGGCCTGTGTGTTTGATAAATTTCTCTGCCATGGTGTGCTCCTTATTTAATGTTGCGAATGTCGGCGAAATGTCCGGTCACAGCAGCAGCGGCAGCCATTGCCGGGCTGACCAGATGCGTGCGCCCGCCGCGCCCCTGGCGGCCTTCAAAGTTACGGTTGCTGATGGAGGCACAACGTTCGCCCGGATTCAGACGGTCGTTGTTCATCGCCAGACACAt                                                                                                                                                                                               >  2:285569/1‑251 (MQ=255)                                                      aCATTGGCGGCATCCAGGGGAACCACCAGGCCTGTGTGTTTGATAAATTTCTCTGCCATGGTGTGCTCCTTATTTAAGGTTGCGAATGTCGGCGAAATGTCCGGTCACAGCAGCAGCGGCAGCCATTGCGGGGTTGACCAGTGGCGTGCGCCCGCCGCGCCCCTGGCGGCCTTCAAAGGTACGGTTGCTGATGGAGGCACAGCGTTCGCGCGGATTCAGACGGTCGTTGTTCATCGCCAGACACATTGagc                                                                                                                                                                                          <  1:188425/251‑1 (MQ=255)                                                      aCATTGGCGGCATCCAGCGGAACCACCAGGCCTGTGTGTTTGATAAATTTCTCTGCCATGGTGTGCTCCTTATTTAATGTTGCGAATGTCGGCGAAATGTCCGGTCACAGCAGCAGCGGCAGCCATTGCCGGGCTGACCAGATGCGTGCGCCCGCCGCGCCCCTGGCGGCCTTCAAAGTTACGGTTGCTGATGGAGGCACAACGTTCGCCCGGATTCAGACGGTCgtt                                                                                                                                                                                                                 >  1:257229/1‑228 (MQ=255)                                                                     aGCGGAACCACCAGGCCTGTGTGTTTGATAAATTTCTCTGCCATGGCGTGCTCCTTATTTAATGTTGCGAATGTCGGCGAAATGTCCGGTCACAGCAGCAGCGGCAGCCATTGCCGGGCTGACCAGATGCGTGCGCCCGCCGCGCCCCTGGCGGCCTTCAAAGTTACGGTTGCTGATGGAGGCACAACGTTCGCCCGGATTCAGACGGTCGTTGTTCATCGCCAGACACATTGAGCAGCCAGGCAAGCGcc                                                                                                                                                                           >  1:57370/1‑251 (MQ=255)                                                                      gCGGAACCACCAGGCCTGTGTGTTTGATAAATTTCTCTGCCATGGTGTGCTCCTTATTTAATGTTGCGAATGTCGGCGAAATGTCCGGTCACAGCAGCAGCGGCAGCCATTGCCGGGCTGACCAGATGCGTGCGCCCGCCGCGCCCCTGGCGGCCTTCAAAGTTACGGTTGCTGATGGAGGCACAACGTTCGCCCGGATTCAGACGGTCGTTGTTCATCGCCAGACACATTGAGCAGCCAGGCAAGCGcc                                                                                                                                                                           >  2:303467/1‑250 (MQ=255)                                                                            ccaccaGGCCTGTGTGTTTGATAAATTTCTCTGCCATGGTGTGCTCCTTATTTAATGTTGCGAATGTCGGCGAAATGTCCGGTCACAGCAGCAGCGGCAGCCATTGCCGGGCTGACCAGATGCGTGCGCCCGCCGCGCCCCTGGCGGCCTTCAAAGTTACGGTTGCTGATGGAGGCACAACGTTCGCCCGGATTCAGACGGTCGTTGTTCATCGCCAGACACATTGAGCAGCCAGGCAAGCGCCATTCaaa                                                                                                                                                                    <  1:285569/251‑1 (MQ=255)                                                                             ccccaGGCCTGGGTGGTGGAGAAATTCCTCTGCCATGGTGGGCTCCTTATTAAATTTTGCGAATGGCGGCGAAATGTCGGGGCACAGAAGCAGCGGCAGCCATTGCCGGGCTGACCAGGTGCGTGCGCCCGCCGCGCCCCTGGCGGCCTTCAAAGTTACGGTTGCTGATGGAGGCACAACGTTCGCCCGGATTCAGACGGTCGTTGTTCATCGCCAGACACATTGAGCAGCCAGGCAAGCGCCATTCaaaa                                                                                                                                                                   <  2:97880/249‑1 (MQ=255)                                                                             caccaGGCCTGTGAGTTTGATAAATTTCTCTGCCATGGTGTGCTCCTTATTTAATGTTGCGAATGTCGGCGAAATGTCCGGTCACAGCAGCAGCGGCAGCCATTGCCGGGCTGACCAGATGCGTGCGCCCGCCGCGCCCCTGGCGGCCTTCAAAGTTACGGTTGCTGATGGAGGCACAACGTTCGCCCGGATTCAGACGGTCGTTGTTCATCGCCAGACACATTGAGCAGCCAGGCAAGCGCCATTCaaaa                                                                                                                                                                   <  1:214118/251‑1 (MQ=255)                                                                                                                    gtgCTCCTTTTTTAATGTTGCGAATGTCGGCGAAATGTCCGGTCACAGCAGCAGCGGCAGCCATTGCCGGGCTGACCAGATGCGTGCGCCCGCCGCGCCCCTGGCGGCCTTCAAAGTTACGGTTGCTGATGGAGGCACAACGTTCGCCCGGATTCAGACGGTCGTTGTTCATCGCCAGACACATTGAGCAGCCAGGCAAGCGCCATTCAAAACCGGCTTCAATAAAGATTTTATCCAGACcttccgcttcc                                                                                                                            <  1:345226/251‑1 (MQ=255)                                                                                                                             attgaatgttgggaagtacGGCGAAAGGCCCGGTCACACCGGCGCCGGCACCCATTGCCGGGTTGACCAGAGGCGTGCGCCCGCCGCGCCCCTGGCGGCCTTCAAAGTTACGGTTGCTGATGGAGGCACAACGTTCGCCCGGATTCAGACGGTCGTTGTTCATCGCCAGACACATTGAGCAGCCAGGCAAGCGCCATTCAAAACCGGCTTCAATAAAGATTTTATCCAGACCTTCCGCTTCCGCCTGGGc                                                                                                                    <  2:293937/232‑1 (MQ=255)                                                                                                                                tAATGTTGCGAAAGAGGGCGAAATGTCCGGTAACAGCAGCAGCGGCAGACATTGCCGGGCTGAACAGATGCGTGCGCCCGACGCGCCCCTGGCGGCCTTCAAAGTTACGGTTGCTGATGGAGGCACAACGTTCGCCCGGATTCAGACGGTCGCTGTTCATCGCCAGACACATTGAGCAGCCAGGCAAGCGCCATTCAAAACAGGCTTCAATAAAGATTTTATCCATACCTTCCGCTTCCACCCGGGCtttt                                                                                                                >  2:235343/1‑251 (MQ=255)                                                                                                                                          aaTGTCGGCGAAATGTCCGGTCACAGCAGCAGCGGCAGCCATTGCCGGGCTGACCAGATGCGTGCGCCCGCCGCGCCCCTGGCGGCCTTCAAAGTTACGGTTGCTGATGGAGGCACAACGTTCGCCCGGATTCAGACGGTCGTTGTTCATCGCCAGACACATTGAGCAGCCAGGCAAGCGCCATTCAAAACCGGCTTCAATAAAGATTTTATCCAGACCTTCCGCTTCCGCCTGGGCTTTTACCGGGCCag                                                                                                      <  2:153034/251‑1 (MQ=255)                                                                                                                                              tCGGCGAAATGTCCGGTCACAGCAGCAGCGGCAGCCATTGCCGGGCTGACCAGATGCGTGCGCCCGCCGCGCCCCTGGCGGCCTTCAAAGTTACGGTTGCTGATGGAGGCACAACGTTCGCCCGGATTCAGACGGTCGTTGTTCATCGCCAGACACATTGAGCAGCCAGGCAAGCGCCATTCAAAACCGGCTTCAATAAAGATTTTATCCAGACCTTCCGCTTCCGCCTGGGCTTTTACCGGGCCAGAGcc                                                                                                  >  1:180476/1‑251 (MQ=255)                                                                                                                                                            ggTCACAGCAGCAGCGGCAGCCATTGCCGGGCTGACCAGATGCGTGCGCCCGCCGCGCCCCTGGCGGCCTTCAAAGTTACGGTTGCTGATGGAGGCACAACGTTCGCCCGGATTCAGACGGTCGTTGTTCATCGCCAGACACATTGAGCAGCCAGGCAAGCGCCATTCAAAACCGGCtt                                                                                                                                                            >  1:340423/1‑179 (MQ=255)                                                                                                                                                            ggTCACAGCAGCAGCGGCAGCCATTGCCGGGCTGACCAGATGCGTGCGCCCGCCGCGCCCCTGGCGGCCTTCAAAGTTACGGTTGCTGATGGAGGCACAACGTTCGCCCGGATTCAGACGGTCGTTGTTCATCGCCAGACACATTGAGCAGCCAGGCAAGCGCCATTCAAAACCGGCtt                                                                                                                                                            <  2:340423/179‑1 (MQ=255)                                                                                                                                                            ggTCACAGCAGCAGCGGCAGCCATTGCCGGGCTGACCAGATGCGTGCGCCCGCCGCGCCCCTGGCGGCCTTCAAAGTTACGGTTGCTGATGGAGGCACAACGTTCGCCCGGATTCAGACGGTCGTTGTTCATCGCCAGACACATTGAGCAGCCAGGCAAGCGCCATTCAAAACCGGCTTCAATAAAGATTTTATCCAGACCTTCCGCTTCCGCCTGGGCTTTTACCGGGCCAGAGCCGGGAACCACCAGTg                                                                                    >  1:61632/1‑251 (MQ=255)                                                                                                                                                             gTCACAGCAGCAGCGGCAGCCATTGCCGGGCTGACCAGATGCGTGCGCCCGCCGCGCCCCTGGCGGCCTTCAAAGTTACGGTTGCTGATGGAGGCACAACGTTCGCCCGGATTCAGACGGTCGTTGTTCATCGCCAGACACATTGAGCAGCCAGGCAAGCGCCATTCAAAACCGGCTTCAATAAAGATTTTATCCAGACCTTCCGCTTCCGCCTGGGCTTTTACCGGGCCAGAGCCGGGAACCACCAGTGc                                                                                   >  1:214190/1‑251 (MQ=255)                                                                                                                                                              tCACAGCAGCAGCGGCAGCCATTGCCGGGCTGACCAGATGCGTGCGCCCGCCGCGCCCCTGGCGGCCTTCAAAGTTACGGTTGCTGATGGAGGCACAACGTTCGCCCGGATTCAGACGGTCGTTGTTCATCGCCAGACACATTGAGCAGCCAGGCAAGCGCCATTCAAAACCGGCTTCAATAAAGATTTTATCCAGACCTTCCGCTTCCGCCTgg                                                                                                                      >  2:94173/1‑215 (MQ=255)                                                                                                                                                              tCACAGCAGCAGCGGCAGCCATTGCCGGGCTGACCAGATGCGTGCGCCCGCCGCGCCCCTGGCGGCCTTCAAAGTTACGGTTGCTGATGGAGGCACAACGTTCGCCCGGATTCAGACGGTCGTTGTTCATCGCCAGACACATTGAGCAGCCAGGCAAGCGCCATTCAAAACCGGCTTCAATAAAGATTTTATCCAGACCTTCCGCTTCCGCCTgg                                                                                                                      <  1:94173/215‑1 (MQ=255)                                                                                                                                                              tCACAGCAGCAGCGGCAGCCATTGCCGGGCTGACCAGATGCGTGCGCCCGCCGCGCCCCTGGCGGCCTTCAAAGTTACGGTTGCTGATGGAGGCACAACGTTCGCCCGGATTCAGACGGTCGTTGTTCATCGCCAGACACATTGAGCAGCCAGGCAAGCGCCATTCAAAACCGGCTTCAATAAAGATTTTATCCAGACCTTCCGCTTCCGCCTGGGCTTTTACCGGGCCAGAGCCGGGAACCACCAGTGcc                                                                                  >  1:347189/1‑251 (MQ=255)                                                                                                                                                                    cagcagcGGCAGCCATTGCCGGGCTGACCAGATGCGTGCGCCCGCCGCGCCCCTGGCGGCCTTCAAAGTTACGGTTGCTGATGGAGGCACAACGTTCGCCCGGATTCAGACGGTCGTTGTTCATCGCCAGACACATTGAGCAGCCAGGCAAGCGCCATTCAAAACCGGCTTCAATAAAGATTTTATCCAGACCTTCCGCTTCCGCCTGGGCTTTTACCGGGCCAGAGCCGGGAACCACCAGTGCCTGCACg                                                                            >  1:175105/1‑251 (MQ=255)                                                                                                                                                                     agcagcGGCAGCCATTGCCGGGCTGACCAGATGCGTGCGCCCGCCGCGCCCCTGGCGGCCTTCAAAGTTACGGTTGCTGATGGAGGCACAACGTTCGCCCGGATTCAGACGGTCGTTGTTCATCGCCAGACACATTGAGCAGCCAGGCAAGCGCCATTCAAAACCGGCTTCAATAAAGATTTTATCCAGACCTTCCGCTTCCGCCTGGGCTTTTACCGGGCCAGAGCCGGGAACCACCAGTGCCTGCACGc                                                                           >  1:174977/1‑251 (MQ=255)                                                                                                                                                                      gcgggggCAGCCATGGCCGGGCTGACCAGAGGGGTGCGCCCGCCGCGCCCCTGGCGGCCTTCAAAGTTACGGTTGCTGATGGAGGCACAACGTTCGCCCGGATTCAGACGGTCGTTGTTCATCGCCAGACACATTGAGCAGCCAGGCAAGCGCCATTCAAAACCGGCTTCAATAAAGATTTTATCCAGACCTTCCGCTTCCGCCTGGGCTTTTACCGGGCCAGAGCCGGGAACCACCAGTGCCTGCACGcc                                                                          <  2:112422/246‑1 (MQ=255)                                                                                                                                                                       cagcGGCAGCCATTGCCGGGCTGACCAGATGCGTGCGCCCGCCGCGCCCCTGGCGGCCTTCAAAGTTACGGTTGCTGATGGAGGCACAACGTTCGCCCGGATTCAGACGGTCGTTGTTCATCGCCAGACACATTGAGCAGCCAGGCAAGCGCCATTCAAAACCGGCTTCAATAAAGATTTTATCCAGACCTTCCGCTTCCGCCTGGGCTTTTACCGGGCCAGAGCCGGGAACCACCAGTGcc                                                                                  >  1:249296/1‑242 (MQ=255)                                                                                                                                                                       cagcGGCAGCCATGGCCGGGCTGCCCAGATGCGTGCGCCCGCCGCGCCCCTGGCGGCCTTCAAAGTTACGGTTGCTGATGGAGGCACAACGTTCGCCCGGATTCAGACGGTCGTTGTTCATCGCCAGACACATTGAGCAGCCAGGCAAGCGCCATTCAAAACCGGCTTCAATAAAGATTTTATCCAGACCTTCCGCTTCCGCCTGGGCTTTTACCGGGCCAGAGCCGGGAACCACCAGTGcc                                                                                  <  2:249296/242‑1 (MQ=255)                                                                                                                                                                          gggCACCCATTGCGGGGCTGCCCAGAGGCGTGCGCCCGCCGCGCCCCTGGCGGCCTTCAAAGTTACGGTTGCTGATGGAGGCACAACGTTCGCCCGGATTCAGACGGTCGTTGTTCATCGCCAGACACATTGAGCAGCCAGGCAAGCGCCATTCAAAACCGGCTTCAATAAAGATTTTATCCAGACCTTCCGCTTCCGCCTGGGCTTTTACCGGGCCAGAGCCGGGAACCACCAGTGCCTGCACGCCTGgc                                                                      <  2:315714/250‑1 (MQ=255)                                                                                                                                                                             cAGCCATTGCCGGGCTGACCAGATGCGTGCGCCCGCCGCGCCCCTGGCGGCCTTCAAAGTTACGGTTGCTGATGGAGGCACAACGTTCGCCCGGATTCAGACGGTCGTTGTTCATCGCCAGACACATTGAGCAGCCAGGCAAGCGCCATTCAAAACCGGCTTCAATAAAGATTTTATCCAGACCTTCCGCTTCCGCCTGGGCTTTTACCGGGCCAGAGCCGGGAACCACCAGTGCCTGCACGCCTGGcgcg                                                                   <  1:79004/251‑1 (MQ=255)                                                                                                                                                                                     gcccggctgaccgtatgctggcacccgccGCGCCCCTGGCGGCCTTCAAAGTTACGGTTGCTGATGGAGGCACAACGTTCGCCCGGATTCAGACGGTCGTTGTTCATCGCCAGACACATTGAGCAGCCAGGCAAGCGCCATTCAAAACCGGCTTCAATAAAGATTTTATCCAGACCTTCCGCTTCCGCCTGGGCTTTTACCGGGCCAGAGCCGGGAACCACCAGTGCCTGCACGCCTGGCGCGACTTTTc                                                            <  2:298425/227‑1 (MQ=255)                                                                                                                                                                                         ggctgcccagcggcgtgcgCCCGCCGCGCCCCTGGCGGCCTTCAAAGTTACGGTTGCTGATGGAGGCACAACGTTCGCCCGGATTCAGACGGTCGTTGTTCATCGCCAGACACATTGAGCAGCCAGGCAAGCGCCATTCAAAACCGGCTTCAATAAAGATTTTATCCAGACCTTCCGCTTCCGCCTGGGCTTTTACCGGGCCAGAGCCGGGAACCACCAGTGCCTGCACGCCTGGCGCGACTTTTCGCCCt                                                       <  2:174977/239‑1 (MQ=255)                                                                                                                                                                                          gCTGACCAGATGCGTGCGCCCGCCGCGCCCCTGGCGGCCTTCAAAGTTACGGTTGCTGATGGAGGCACAACGTTCGCCCGGATTCAGACGGTCGTTGTTCATCGCCAGACACATTGAGCAGCCAGGCAAGCGCCATTCAAAACCGGCTTCAATAAAGATTTTATCCAGACCTTCCGCTTCCGCCTGGGCTTTTACCGGGCCAGAGCCGGGAACCACCAGTGCCTGCACGCCTGGCGCGACTTTTCGCCCtt                                                      >  2:268213/1‑251 (MQ=255)                                                                                                                                                                                                            cccgccGCGCCCCTGGCGGCCTTCAAAGTTACGGTTGCTGATGGAGGCACAACGTTCGCCCGGATTCAGACGGTCGTTGTTCATCGCCAGACACATTGAGCAGCCAGGCAAGCGCCATTCAAAACCGGCTTCAATAAAGATTTTATCCAGACCTTCCGCTTCCGCCTGGGCTTTTACCGGGCCAGAGCCGGGAACCACCAGTGCCTGCACGCCTGGCGCGACTTTTCGCCCTTTGGCGATCTCCGCTGCcg                                    <  1:358577/251‑1 (MQ=255)                                                                                                                                                                                                                  gcgcCCCTGGCGGCCTTCAAAGTTACGGTTGCTGATGGAGGCACAACGTTCGCCCGGATTCAGACGGTCGTTGTTCATCGCCAGACACATTGAGCAGCCAGGCAAGCGCCATTCAAAACCGGCTTCAATAAAGATTTTATCCAGACCTTCCGCTTCCGCCTGGGCTTTTACCGGGCCAGAGCCGGGAACCACCAGTGCCTGCACGCCTGGCGCGACTTTTCGCCCTTTGGCGATCTcc                                           <  1:47834/238‑1 (MQ=255)                                                                                                                                                                                                                  gcgcCACTGGCGGCCTTCAAAGGTAAGGTTGCTGATGGAGGCACAACGTTCGCCCGGATTCAGACGGTCGTTGTTCATCGCCAGACACATTGAGCAGCCAGGCAAGCGCCATTCAAAACCGGCTTCAATAAAGATTTTATCCAGACCTTCCGCTTCCGCCTGGGCTTTTACCGGGCCAGAGCCGGGAACCACCAGTGCCTGCACGCCTGGCGCGACTTTTCGCCCTTTGGCGAGCTcc                                           >  2:47834/1‑238 (MQ=255)                                                                                                                                                                                                                              gcCTTCAAAGTTACGGTTGCTGAAGGAGGCACAACGTTCGCGCGGATCCAGACGGTCGTTGTTCATCGCCAGACACATTGAGCAGCCAGGCAAGCGCCATTCAAAACCGGCTTCAGTAAAGATGTTATCCAGACCTTCCGCTTTCGCCGGGGCTTTTACCTTGCCAGGGTCGGGACCCACCAGTGCCTGCACGCCTGTCGCGACTTTTCGCCTTTTGGCGATCTCCGCTTCCGCGCGCAATTCTTCAATgc                  <  2:343201/251‑1 (MQ=255)                                                                                                                                                                                                                                       gTTACGGTTGCTGATGGAGGCACAACGTTCGCCCGGATTCAGACGGTCGTTGTTCATCGCCAGACACATTGAGCAGCCAGGCAAGCGCCATTCAAAACCGGCTTCAATAAAGATTTTATCCAGACCTTCCGCTTCCGCCTGGGCTTTTACCGGGCCAGAGCCGGGAACCACCAGTGCCTGCACGCCTGGCGCGACTTTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTgg         >  1:347021/1‑251 (MQ=255)                                                                                                                                                                                                                                               tGCTGATGGAGGCACAACGTTCGCCCGGATTCAGACGGTCGTTGTTCATCGCCAGACACATTGAGCAGCCAGGCAAGCGCCATTCAAAACCGGCTTCAATAAAGATTTTATCCAGACCTTCCGCTTCCGCCTGGGCTTTTACCGGGCCAGAGCCGGGAACCACCAGTGCCTGCACGCCTGGCGCGACTTTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGa  >  1:174577/1‑250 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     CCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCGACATTGGCGGCATCCAGCGGAACCACCAGGCCTGTGTGTTTGATAAATTTCTCTGCCATGGTGTGCTCCTTATTTAATGTTGCGAATGTCGGCGAAATGTCCGGTCACAGCAGCAGCGGCAGCCATTGCCGGGCTGACCAGATGCGTGCGCCCGCCGCGCCCCTGGCGGCCTTCAAAGTTACGGTTGCTGGTGGAGGCACAACGTTCGCCCGGATTCAGACGGTCGTTGTTCATCGCCAGACACATTGAGCAGCCAGGCAAGCGCCATTCAAAACCGGCTTCAATAAAGATTTTATCCAGACCTTCCGCTTCCGCCTGGGCTTTTACCGGGCCAGAGCCGGGAACCACCAGTGCCTGCACGCCTGGCGCGACTTTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGA  >  NC_000913/79342‑79829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑