Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	78,533	A→C	M305L (ATG→CTG)	setA →	sugar exporter SetA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	78,533	0	A	C	100.0%	75.2 / NA	24	M305L (ATG→CTG)	setA	sugar exporter SetA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (9/15);  total (9/15)

ACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCG  >  NC_000913/78305‑78779                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       aCCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACt                                                                                                                                                                                                                                  <  2:66645/251‑1 (MQ=255)     gCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACttt                                                                                                                                                                                                                                <  2:14173/249‑1 (MQ=255)                   attattGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTAt                                                                                                                                                                                                                <  1:314958/251‑1 (MQ=255)                         gATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCAt                                                                                                                                                                                                          <  2:16996/251‑1 (MQ=255)                                       gATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTGTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTg                                                                                                                                                                                            <  1:209883/251‑1 (MQ=255)                                                   gTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCCGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTTTGGTAAGCGGCGAATGATGGTCATAGCAGGGGCGGCAGGAGTACTGTTTTACCCCGGAATGATTTTATTTCATAACCGTCTGGCGTTGATGACGCTGCAAATTTTTAACGCTGTATTTATCGGCATTGTTTCGGGGATTGGGATGCTATggg                                                                                                                                                                                >  1:247801/1‑250 (MQ=255)                                                                          cGGGTTTCCTGATGGGGACGGCCGCTGGTCTGGAACTACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCTTAGCCGTCTGGCGGTGATGACGCTGCAACTTTTTCACGCTGTATTTATCGGCATTGTTGCGGGTTTTGGGGTGCTCTGGTTTAAGGATTTAATGCCTGGAagg                                                                                                                                                         >  2:37500/1‑250 (MQ=255)                                                                                            cGGCAGCTGGTCTGGAAATACCAGCAATGAGTCTGGCTGGCGACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGAGGACGCTGCAACTTTTTAACGCTGTATGTATCGGCATTGTTGCGGGTATTGGGACGCTATGGTTTCAGGATTTAAGGCCTGGAAGAGCGGGGGCAGCTaccacc                                                                                                                                       <  2:273846/251‑1 (MQ=255)                                                                                                    ggtctggaAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGCCGCTGCCACTTTTTAACGCTTTATTTATCGGCCTTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCCCCTTATTTAc                                                                                                                               >  2:309380/4‑251 (MQ=255)                                                                                                     gtctggaAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACt                                                                                                                              >  1:351972/3‑251 (MQ=255)                                                                                                             aTACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTAt                                                                                                                      <  2:351972/251‑1 (MQ=255)                                                                                                                     aaTGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCg                                                                                                              <  1:190053/251‑1 (MQ=255)                                                                                                                                      actaTGTCAAACGTTCTGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCtggctggc                                                                                             >  2:22364/1‑251 (MQ=255)                                                                                                                                                                  aaTGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGGATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACCAAGtt                                                                 >  2:346626/1‑251 (MQ=255)                                                                                                                                                                                      cggcAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTAc                                             <  2:306611/251‑1 (MQ=255)                                                                                                                                                                                           ggAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGc                                                                                                                                                       >  1:154889/1‑140 (MQ=255)                                                                                                                                                                                           ggAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGc                                                                                                                                                       <  2:154889/140‑1 (MQ=255)                                                                                                                                                                                             aGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTaa                                      <  1:368650/251‑1 (MQ=255)                                                                                                                                                                                                   gTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCgg                                >  1:133314/1‑251 (MQ=255)                                                                                                                                                                                                       tACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTa                             <  1:346626/250‑1 (MQ=255)                                                                                                                                                                                                                   cttttATTTCATAGCCGTCTGGCGTTGATGCAGCTGCAACTTTTTAACGCTGTATATATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACAACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAAtt                                    >  1:225368/2‑231 (MQ=255)                                                                                                                                                                                                                   attTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTATCGc                <  1:22364/251‑1 (MQ=255)                                                                                                                                                                                                                   attTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTGTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATGTACTAACAGTATTTCGACCGGGGTAATTCTGGCGGGCGTTATTCAGGGAGCAATTACACAAAGTTGGGGGCACTTTGCTGTGTACTGGGTAAtg                                    <  2:225368/231‑2 (MQ=255)                                                                                                                                                                                                                                 ccGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGTCGTTATTCAGGGAGCAATTTCACAAAGTTGGGGGCACTTTTCTGTCTACTGGGTAATTGCGGTTATTTCTGTTATCGCATTATTTTTAACCg  <  1:212860/251‑1 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       ACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCG  >  NC_000913/78305‑78779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑