Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 78,406 A→T G262G (GGA→GGT)  setA → sugar exporter SetA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091378,4060AT91.3% 64.4 / ‑5.7 23G262G (GGA→GGT) setAsugar exporter SetA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (5/16);  minor base G (2/0);  total (7/16)
Fisher's exact test for biased strand distribution p-value = 8.30e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TATTCACACTCAGTCTGGTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGTAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACC  >  NC_000913/78159‑78646
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                
tatTCACACTCAGTCTGGTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGTAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGGCGGCAGctggtctg                                                                                                                                                                                                                                               >  2:368650/1‑251 (MQ=255)
   tCACACTCAGTCTGGTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGTAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGctggtctgga                                                                                                                                                                                                                                             <  2:82729/250‑1 (MQ=255)
    cacccTCAGTCTGGTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGTAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGaa                                                                                                                                                                                                                                            <  2:45399/246‑1 (MQ=255)
                   attgattgCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGTAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGGTTTCTGATGGGGGCGGCAGCTGGTCTGGAAATACCAGCAATGAttt                                                                                                                                                                                                                            >  2:68068/1‑250 (MQ=255)
                        ttgCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGTAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGAGGGGGACGGCAGCTGGGCTGGAAATACCAGCAATGATTctgggt                                                                                                                                                                                                                       >  1:306611/1‑249 (MQ=255)
                         tgCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGTAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTctggct                                                                                                                                                                                                                       <  1:283175/250‑1 (MQ=255)
                          gCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGTAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTctggctgg                                                                                                                                                                                                                     <  1:129728/251‑1 (MQ=255)
                                      ccGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGTAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCa                                                                                                                                                                                                          <  1:87495/250‑1 (MQ=255)
                                                    ggTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGTATAGTAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCGGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGTATTGCCAGACAAACTGGCGGGTTGCCTGATGGTGACGTCAGCTGTTCTGTAAATAGCAGCTGTGATTCTGGCTGGCTACGATGTCAAACGTTATGGTAAgc                                                                                                                                                                                           <  1:219296/251‑1 (MQ=255)
                                                             gCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGTAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAat                                                                                                                                                                                    <  1:171448/249‑1 (MQ=255)
                                                             gCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGTAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAat                                                                                                                                                                                    <  1:68068/249‑1 (MQ=255)
                                                                     aaaaTGCTTTATCAATGCAAGGTGGCTGGCAGGATAGTAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATTGGGACGGCAGCTGGTCTGGAAATACCAGCAATGAGTCTGGCTGGCTACTATGTCAAACGTTATGGTAATCGGCGAAGGATGGTCATa                                                                                                                                                                          <  2:44825/251‑1 (MQ=255)
                                                                              tATCAATGCAAGGTGGCTGGCAGGATAGTAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTggc                                                                                                                                                                  <  2:325791/250‑1 (MQ=255)
                                                                                                               tACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGTTGGGGACGGCAGCTGGTCTGGAAATACCCGCAATGATTCTGGCTGGCTACTATTTCAAACGGTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGTGTACTGGTTTACACCGGATTGAatttt                                                                                                                                >  1:225/1‑250 (MQ=255)
                                                                                                                                        gTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCCGGTGGGCTGGAAATACCAGCAATTATTCTGGCTGGCTACTATGTTAAACGTTATGGTAATCGGCGGATGATGGTTATAGCAGTGGCGGCAGGAGGACTGTTTTATACCGGATTGTTTTTTTTTTCTAGCCgctgtggggtgttgg                                                                                                       >  2:193465/1‑237 (MQ=255)
                                                                                                                                        gTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCCAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCa                                                                                                                                                              >  1:121692/1‑196 (MQ=255)
                                                                                                                                        gTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCa                                                                                                                                                              <  2:121692/196‑1 (MQ=255)
                                                                                                                                                  aCCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACt                                                                                             <  2:66645/251‑1 (MQ=255)
                                                                                                                                                      gCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACttt                                                                                           <  2:14173/249‑1 (MQ=255)
                                                                                                                                                                    attattGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTAt                                                                           <  1:314958/251‑1 (MQ=255)
                                                                                                                                                                          gATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCAt                                                                     <  2:16996/251‑1 (MQ=255)
                                                                                                                                                                                        gATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTGTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTg                                                       <  1:209883/251‑1 (MQ=255)
                                                                                                                                                                                                    gTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCCGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTTTGGTAAGCGGCGAATGATGGTCATAGCAGGGGCGGCAGGAGTACTGTTTTACCCCGGAATGATTTTATTTCATAACCGTCTGGCGTTGATGACGCTGCAAATTTTTAACGCTGTATTTATCGGCATTGTTTCGGGGATTGGGATGCTATggg                                           >  1:247801/1‑250 (MQ=255)
                                                                                                                                                                                                                           cGGGTTTCCTGATGGGGACGGCCGCTGGTCTGGAACTACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCTTAGCCGTCTGGCGGTGATGACGCTGCAACTTTTTCACGCTGTATTTATCGGCATTGTTGCGGGTTTTGGGGTGCTCTGGTTTAAGGATTTAATGCCTGGAagg                    >  2:37500/1‑250 (MQ=255)
                                                                                                                                                                                                                                             cGGCAGCTGGTCTGGAAATACCAGCAATGAGTCTGGCTGGCGACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGAGGACGCTGCAACTTTTTAACGCTGTATGTATCGGCATTGTTGCGGGTATTGGGACGCTATGGTTTCAGGATTTAAGGCCTGGAAGAGCGGGGGCAGCTaccacc  <  2:273846/251‑1 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                
TATTCACACTCAGTCTGGTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGTAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACC  >  NC_000913/78159‑78646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑