Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	75,707	C→T	L531L (CTG→CTA)	sgrR ←	DNA‑binding transcriptional dual regulator SgrR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	75,707	0	C	T	100.0%	135.2 / NA	41	L531L (CTG→CTA)	sgrR	DNA‑binding transcriptional dual regulator SgrR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/12);  total (29/12)

ACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAA‑T‑GGCACCATCGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGATTCATCTCGCCATTGCGCCAGCGAGCAGCGTCGGCTTGCCAGTCAATGGGAATGCAATGTTGTAGCAGTGGCACTTCGCATAAATGTGCGAAAACAGAGAAGTCCAGCGGCAGGGTAAAGTTGGCGCTGTTTAGCCAGATATCAC  >  NC_000913/75466‑75951                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                       aCATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGTTATTCATGCGTAGGCCgcgc                                                                                                                                                                                                                                               >  1:161955/1‑251 (MQ=255)                   gCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTg                                                                                                                                                                                                                              >  2:91638/1‑250 (MQ=255)                               agagTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGcc                                                                                                                                                                                                                   >  2:270432/1‑249 (MQ=255)                                gagTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAgt                                                                                                                                                                                                                >  2:50712/1‑251 (MQ=255)                                 cgTGGAAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATAAGCCAgtg                                                                                                                                                                                                               >  2:46684/2‑251 (MQ=255)                                  gTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAgtgg                                                                                                                                                                                                              >  1:68985/1‑251 (MQ=255)                                     gCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAgtggtgg                                                                                                                                                                                                           <  1:52759/251‑2 (MQ=255)                                       aaaGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAgtggtggat                                                                                                                                                                                                         >  1:110335/1‑248 (MQ=255)                                       aaaGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGATATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAgtggtggat                                                                                                                                                                                                         >  1:40041/1‑248 (MQ=255)                                                 agaAGGAGCCTCAAATCCCTTCGACGGCGTTACCCGGATCAGGTTCGACGGGCATTTTCTCAGCGCACGCGTACGCGTGGCACCACGTTGAGACCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGACTGATCAGCCAgtggtggataagcggcacc                                                                                                                                                                                               >  2:375324/1‑238 (MQ=255)                                                               aTCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAA‑TCGGCACCATTGCTTTGCTGGc                                                                                                                                                                               <  2:132737/251‑1 (MQ=255)                                                                       gCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGC‑GGCACCATTGCTTTGCTGGCGACCAGtt                                                                                                                                                                       >  2:318197/1‑251 (MQ=255)                                                                                     gATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGC‑GGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTc                                                                                                                                                         >  2:117864/1‑251 (MQ=255)                                                                                                aCGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGC‑GGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCAt                                                                                                                                              >  1:5144/1‑251 (MQ=255)                                                                                                      aTTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGC‑GGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGcc                                                                                                                                        >  2:244942/1‑251 (MQ=255)                                                                                                       ttttCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGC‑GGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGcc                                                                                                                                        >  1:274211/1‑250 (MQ=255)                                                                                                                             gtgacACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGC‑GGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCgg                                                                                                                 >  2:323605/5‑251 (MQ=255)                                                                                                                                  aCCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCACTCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGC‑GGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGc                                                                                                            >  1:188171/1‑251 (MQ=255)                                                                                                                                  aCCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGC‑GGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGc                                                                                                            >  1:151968/1‑251 (MQ=255)                                                                                                                                       gTTGAGAACGGCGTTAGTGTATTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGC‑GGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTc                                                                                                       <  1:99391/251‑1 (MQ=255)                                                                                                                                          gagaACGGCGTTAGTGTAGTGATTTTGTTATAAACCAGCAATAATGGATCCGGAGGAGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGAATTCATGCGTAGGCCGCGCATACTGCGTTGACCATGAATGATCAGCCAGTGGTG‑GATAAGC‑GGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGACAGCGGGCGGCGTCGGCTCGCCAGTcgat                                                                                                    >  1:354157/1‑248 (MQ=255)                                                                                                                                              aCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGC‑GGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAggcgatcgg                                                                                                 >  2:240787/1‑242 (MQ=255)                                                                                                                                                 gCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGC‑GGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAAta                                                                                             <  1:350113/251‑2 (MQ=255)                                                                                                                                                           agtgATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGC‑GGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGTTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGtgttgga                                                                                   <  2:304358/251‑3 (MQ=255)                                                                                                                                                                    gTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGC‑GGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGc                                                                           >  2:28224/1‑250 (MQ=255)                                                                                                                                                                      tATCAACCAGCAATCCTGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATCCTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGC‑GGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGACTACAGTGTTGGAGCAGCGGCAcc                                                                        >  2:178201/1‑250 (MQ=255)                                                                                                                                                                      tATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGC‑GGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCAcc                                                                        >  1:265702/1‑250 (MQ=255)                                                                                                                                                                                cAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGC‑GGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCacaagt                                                              >  2:69554/1‑246 (MQ=255)                                                                                                                                                                                       ggATCAGGGGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGC‑GGCACCATTGCTTTGATGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGTGGCGTCGGCTTGCCTGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCacaagtgagaga                                                        >  2:315262/1‑239 (MQ=255)                                                                                                                                                                                         aTCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGC‑GGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGaac                                                      >  1:196393/1‑249 (MQ=255)                                                                                                                                                                                                    cAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAAGGATCAGCCAGTGGTG‑GATAAGC‑GGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTGCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGAGCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGaacagcgaaaaatcc                                          <  1:31231/251‑14 (MQ=255)                                                                                                                                                                                                            gCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCACTGAATGATCAGCCAGTGGTG‑GATAAGC‑GGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGaacagcgaaaaatccagcggc                                    >  1:371068/1‑230 (MQ=255)                                                                                                                                                                                                               gATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGC‑GGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGCGAAAAATCCAGCGGCAgc                                 <  2:165527/249‑2 (MQ=255)                                                                                                                                                                                                                    aaaaTCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGC‑GGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACACGTGCGCGAACAGCGAAAAATCCAGCGGCAGCGTAAAGt                          >  2:247681/1‑251 (MQ=255)                                                                                                                                                                                                                    aaaaTCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGC‑GGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGCGAAAAATCCAGCGGCAGCGTAAAGt                          <  2:265702/251‑1 (MQ=255)                                                                                                                                                                                                                      aaTCGAACCAGCCGAGGGTAGTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGC‑GGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGCGAAAAATCCAGCGGCAGCGTAAAGTTg                        <  1:235454/251‑1 (MQ=255)                                                                                                                                                                                                                       agctaaCCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGAGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGC‑GGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGCGAAAAATCCAGCGGCAGCGTAAAGTTgg                       <  2:98892/247‑1 (MQ=255)                                                                                                                                                                                                                           aaCCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGC‑GGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGCGAAAAATCCAGCGGCAGCGTAAAGTTGgcgat                   >  1:98892/1‑249 (MQ=255)                                                                                                                                                                                                                                     gggTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGC‑GGCACCAGTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTGGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGCGAAAAAGCCAGCGGCAGCGTAAAGTTGGCGCTGGTAAGCCAg         <  2:372285/251‑1 (MQ=255)                                                                                                                                                                                                                                      ggTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGC‑GGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAAGCCAGa        <  2:274211/251‑1 (MQ=255)                                                                                                                                                                                                                                            cATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGC‑GGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAAGCCAGATATcgc  >  2:103779/1‑249 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                       ACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAA‑T‑GGCACCATCGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGATTCATCTCGCCATTGCGCCAGCGAGCAGCGTCGGCTTGCCAGTCAATGGGAATGCAATGTTGTAGCAGTGGCACTTCGCATAAATGTGCGAAAACAGAGAAGTCCAGCGGCAGGGTAAAGTTGGCGCTGTTTAGCCAGATATCAC  >  NC_000913/75466‑75951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑