Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	73,868	C→G	G218G (GGG→GGC)	thiP ←	thiamine ABC transporter membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	73,868	0	C	G	100.0%	132.0 / NA	41	G218G (GGG→GGC)	thiP	thiamine ABC transporter membrane subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (18/23);  total (18/23)

AGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAGCATTGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTCGCCTGCGGACCGCCCCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGCGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCTACGCATCCCAAGCTGGGCGGCAAGTTGACGCTGTTCGCCGGGGATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCAGATTAAAAAACACATGGGCCAGCAAAATACCTT  >  NC_000913/73623‑74103                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                            agcagcGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAg                                                                                                                                                                                                                                        <  2:128752/251‑1 (MQ=255)                    acacCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACACGAGGCAAAACAACATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGa                                                                                                                                                                                                                       >  1:300903/1‑249 (MQ=255)                       ccGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTg                                                                                                                                                                                                                  <  1:230326/251‑1 (MQ=255)                             cGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGaa                                                                                                                                                                                                             >  2:141999/1‑250 (MQ=255)                                aaaTGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTTGTTGCCTTCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACag                                                                                                                                                                                                         <  1:359611/251‑1 (MQ=255)                                      ggCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGGGGCGAAGCTGGCGAAACAGAGCAt                                                                                                                                                                                                    >  2:294968/1‑250 (MQ=255)                                        cTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATaa                                                                                                                                                                                                  >  2:170617/1‑250 (MQ=255)                                                        ccGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAAcc                                                                                                                                                                                 <  1:347080/251‑1 (MQ=255)                                                                cgcCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGAt                                                                                                                                                                         <  1:177623/251‑1 (MQ=255)                                                                         tGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGGTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGTCGAAACAGAGCATAATGATAAGTGCAGCAACCGGCGGGATTTGTCGcc                                                                                                                                                                 <  1:25431/250‑1 (MQ=255)                                                                              cagcGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAAcc                                                                                                                                                           <  1:26401/251‑1 (MQ=255)                                                                                 cGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGAATTGTCGCCGTAACCACg                                                                                                                                                        >  1:58095/1‑251 (MQ=255)                                                                                                  tGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGAt                                                                                                                                                                         >  2:96939/1‑217 (MQ=255)                                                                                                  tGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGAt                                                                                                                                                                         <  1:96939/217‑1 (MQ=255)                                                                                                   ggCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGcccattgaagaagcggg                                                                                                                                      >  2:370315/1‑236 (MQ=255)                                                                                                                          gcatcACCAACCCGAGGCAACACGCCAGCTGGATCAGCGCCAGCATCGCCGCGCGGGCATGTTCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGTTTGCCGGCGGACCTCCGCCCAGCTATAGCACGGTGGCGAAGCTGGCGAAACAGAGCGTAAAGATAAGTTCAGCAACCGGCGTGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCa                                                                                                               <  1:243883/247‑1 (MQ=255)                                                                                                                          gcAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCCGCATAGCAGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGAGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCCACGAAGCGGAAAAAATGCCAGCAGCGCATccc                                                                                                                >  1:154657/2‑250 (MQ=255)                                                                                                                              cACCAACCCGAGGCAACCCACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGAGGAAAAAATGCCCGCCGCGCATCCCCAGcc                                                                                                           >  2:168526/1‑250 (MQ=255)                                                                                                                                        aGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGGGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCAGCGCCTCCCCAGCTGggcggcc                                                                                                   >  2:93826/1‑248 (MQ=255)                                                                                                                                            aacacaCCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCCGCCGCGCCTCCCAAGCTGGGCGGCAAgctg                                                                                              >  2:328184/3‑247 (MQ=255)                                                                                                                                                 acCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACgttg                                                                                        >  1:70996/1‑248 (MQ=255)                                                                                                                                                  ccATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGCAACTCAGCGCCGGATAGATTGCCAGCTCGATAGTGGTGGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGGAACCACGGCCATTCGACGAAGCGGAAAAAAGGCCGGCCGCGCATCCCAAGCTGGGCGGCAGGCTGACGTTGt                                                                                       <  2:219680/251‑1 (MQ=255)                                                                                                                                                        ggatcagcGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGcc                                                                                  <  1:142916/246‑1 (MQ=255)                                                                                                                                                                gcCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGGTGTTCGCCGGGAATGtt                                                                         >  2:166398/1‑251 (MQ=255)                                                                                                                                                                 ccAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGttt                                                                        <  2:264699/251‑1 (MQ=255)                                                                                                                                                                    gCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCCTTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCCAGCTGACGATGTTCGCCGGGGATGTTTTcc                                                                     >  2:318492/1‑251 (MQ=255)                                                                                                                                                                      atcgCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCCTTCGACGAAGCGGAAAAAATGCCa                                                                                                                            >  2:202786/4‑194 (MQ=255)                                                                                                                                                                      atcgCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCa                                                                                                                            <  1:202786/191‑1 (MQ=255)                                                                                                                                                                      atcgCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAg                                                                   <  2:293835/248‑1 (MQ=255)                                                                                                                                                                        cgCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTg                                                                 <  1:214889/250‑1 (MQ=255)                                                                                                                                                                                  gCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGcc                                                                                                                             <  1:330734/181‑1 (MQ=255)                                                                                                                                                                                  gCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGcc                                                                                                                             >  2:330734/1‑181 (MQ=255)                                                                                                                                                                                            aGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTc                                             <  1:88793/251‑1 (MQ=255)                                                                                                                                                                                                      cAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCTGCTCGCCATCggt                                    <  1:65863/250‑2 (MQ=255)                                                                                                                                                                                                      cAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCggta                                   <  1:116534/251‑3 (MQ=255)                                                                                                                                                                                                        gcgcCTGATAGATTGCCAGCTCGATGGTGGTTGCCTGCGGGCCGCCGCCCAGCGATAGCGCGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGGTGTTCGCCGGGAATGTTTTCCAGTGTGTGGAGTAATAAGCGGCTCgtcagctgtaga                                 <  2:341432/251‑12 (MQ=255)                                                                                                                                                                                                        gcgcCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGa                                 <  1:22228/251‑1 (MQ=255)                                                                                                                                                                                                           ccTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGAtt                               >  1:117806/1‑250 (MQ=255)                                                                                                                                                                                                                     tGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTATATTaaaaaatacg                     <  1:302536/250‑5 (MQ=255)                                                                                                                                                                                                                     tGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAACGGAAAAAATGACAGCCGCGCATCCCCAGCTGGGAGGCAAGCTGACGTTGTTCTCCGGGAATGCTTTCCAGTGCCTGGAGTAATCAACGGCTCGCCCTCGGGAGATTaaaaaatacgg                    >  2:2166/1‑246 (MQ=255)                                                                                                                                                                                                                                        gCCTGAGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCtt  <  1:201884/250‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                            AGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAGCATTGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTCGCCTGCGGACCGCCCCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGCGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCTACGCATCCCAAGCTGGGCGGCAAGTTGACGCTGTTCGCCGGGGATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCAGATTAAAAAACACATGGGCCAGCAAAATACCTT  >  NC_000913/73623‑74103

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑