Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	64,794	T→C	A329A (GCA→GCG)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	64,794	0	T	C	97.4%	124.8 / NA	39	A329A (GCA→GCG)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (16/22);  total (16/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAGCTCCTGAGCGACAGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGAC‑GCCGTTTTTAAAGCCGTGCTCG  >  NC_000913/64560‑65013                                                                                                                                                                                                                                           |                                                                                                                                                                                                                             ggAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAGCGCCGCCGCCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCTCTCGTTCGAGTAAAAATTGCATGATTGCAGTTTTATGGAAAATCTGCGTCACCAGCTCGTAGTCTTTCAGGTTAGAAGTTGCCAGCGCAGGTTTAt                                                                                                                                                                                                              <  2:164920/250‑1 (MQ=255)             gTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGGTATAAGTCGACAGCGAAGGTTTATCTTCGGCGAAAc                                                                                                                                                                                                  <  2:263240/251‑1 (MQ=255)                        ggCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAAtt                                                                                                                                                                                       <  2:222898/251‑1 (MQ=255)                            ggCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGt                                                                                                                                                                                   <  1:56025/251‑1 (MQ=255)                                        cACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCCGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCCTTCGATccc                                                                                                                                                                       >  2:15857/1‑251 (MQ=255)                                                    aTTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGa                                                                                                                                                           <  2:91273/251‑1 (MQ=255)                                                          cgcgACATAGCCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCATTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCTATCCCACGGGTTATCGATAGAtt                                                                                                                                                     <  2:113841/251‑1 (MQ=255)                                                                     cAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCTCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAATGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCt                                                                                                                                          <  1:207536/251‑1 (MQ=255)                                                                          cggtGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATa                                                                                                                                      >  1:135159/4‑250 (MQ=255)                                                                            gtGCATACGAGGAAAATAGAGATGACCAAACGCCGCCGCCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGGTATAAGTCGCCAGCGCAGGGTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGAGCCCACGGGTTATCGATGGATTTTCCATGGCCTAATaact                                                                                                                                   <  2:239809/250‑4 (MQ=255)                                                                             ggcatacgaggAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCGCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTc                                                                                                                                  <  2:135159/242‑1 (MQ=255)                                                                                     aggAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCg                                                                                                                           >  1:126430/2‑250 (MQ=255)                                                                                     aggAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGGCCATTTCGTCCATTCGATCCCCAGGGTTATCGATAGATTTTTCTTCGCCTAATaaatcctgtgcg                                                                                                                           >  2:197687/2‑239 (MQ=255)                                                                                          aaTAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGtt                                                                                                                     <  2:126430/251‑1 (MQ=255)                                                                                             agagATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTc                                                                                                                   <  1:46837/250‑1 (MQ=255)                                                                                              gagaTGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTGCGCCTAATAACTCCTGAGCGACGGTTTCCa                                                                                                                 <  2:337307/251‑1 (MQ=255)                                                                                               agaTGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAg                                                                                                                <  1:2767/251‑1 (MQ=255)                                                                                                gaTGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGc                                                                                                               <  1:197687/251‑1 (MQ=255)                                                                                                      cAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAAt                                                                                                         >  1:307096/1‑251 (MQ=255)                                                                                                             cgccACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAag                                                                                                     >  1:35513/1‑248 (MQ=255)                                                                                                                cACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAAt                                                                                               >  1:206995/1‑251 (MQ=255)                                                                                                                     atcccccgTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAg                                                                                          <  1:188806/246‑1 (MQ=255)                                                                                                                       cccccgTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGa                                                                                         <  1:15857/247‑1 (MQ=255)                                                                                                                        ccccgTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTTCTTCGCCTAATAACTCCTGAGCGGCGGTTTTCAGCGAGAATGAAGAGAAATTCCAGAAc                                                                                       >  2:237147/3‑251 (MQ=255)                                                                                                                              ggcGGTCTACCGGCAGGACGGTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATTTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGCCAAATTCGTCCATTTGCTCCCACGGGGTATTGGTAGATTTTCCTTCTCCTAATTACTTCTGAGCGAGGGTTTCCAACGAGAAAGAAGAGAAATTCACGAACGCggtt                                                                                 >  2:361571/3‑249 (MQ=255)                                                                                                                                      aCCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAg                                                                            <  1:91351/248‑1 (MQ=255)                                                                                                                                                   tCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACTCGGATTTCAGCGCCTCGATACCGTc                                                             <  2:358622/250‑1 (MQ=255)                                                                                                                                                                    aGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTc                                                             >  1:347214/1‑233 (MQ=255)                                                                                                                                                                    aGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTc                                                             <  2:347214/233‑1 (MQ=255)                                                                                                                                                                       aaaaaTGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTAGCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGc                                        <  1:148345/251‑1 (MQ=255)                                                                                                                                                                                  gATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCaaaa                              >  2:70746/1‑250 (MQ=255)                                                                                                                                                                                      tCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGCCCTTTAGCCTGGGCAAAAAAGa                          <  1:72180/250‑1 (MQ=255)                                                                                                                                                                                       cAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAACTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGAC‑g                       >  1:185781/1‑251 (MQ=255)                                                                                                                                                                                                  aaaaTCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGAC‑GCCGTTTTTaca            <  1:286595/248‑3 (MQ=255)                                                                                                                                                                                                          cGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGAC‑GCCGTTTTTAAAGCCGTGCt    <  2:357006/251‑1 (MQ=255)                                                                                                                                                                                                           gTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGACATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGAC‑GCCGTTTTTAAAGCCGTGCTc   >  2:332565/1‑251 (MQ=255)                                                                                                                                                                                                           gTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGAC‑GCCGTTTTTAAAGCCGTGCTc   >  2:11419/1‑251 (MQ=255)                                                                                                                                                                                                            tCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTCTCGATAGATTTTCCTTCGCCTAATAACTTCTGAGCGACGGGTTCCAGTGGGAATGAAGAGAAATTCCAGAACGCGGATTTCAGGGCCTCGATCACGTGGATAATTAACCGGACTTTAGCCTGGGCAAAAAA‑ACAGCCGGTTTTAAAGACGAGCTcc  >  2:239593/1‑250 (MQ=255)                                                                                                                                                                                                            tCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGGTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGCTAATTATCCGACCTTTAGCCTGGGCAAAAAAGCC‑GCCGGTTTTAAAGCCGTGCTcg  >  2:271331/1‑251 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                             TGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAGCTCCTGAGCGACAGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGAC‑GCCGTTTTTAAAGCCGTGCTCG  >  NC_000913/64560‑65013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑