Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	73,331	A→G	T397T (ACT→ACC)	thiP ←	thiamine ABC transporter membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	73,331	0	A	G	100.0%	158.7 / NA	47	T397T (ACT→ACC)	thiP	thiamine ABC transporter membrane subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/13);  total (34/13)

AAAATCACCAATCGACAGCACGCATGCAAAGGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCCACCACTTTTAAGCGTGACCAGCCTTCAATCCCCAGCGACTGACATAACATGCTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGCACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGTGGCAGGCCGATAG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGAT‑CAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTG  >  NC_000913/73091‑73573                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     aaaaTCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGG‑TGttgttg                                                                                                                                                                                                                                            >  1:345307/1‑250 (MQ=255)  aaaTCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCTCTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCATGCCAATGG‑TGTTGTTGAg                                                                                                                                                                                                                                          <  2:326340/251‑1 (MQ=255)         cAATCGACAGAACGCAGGAAAAAGCCAGCGCCTGCGCCAGTGGACGTGTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTTACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGT‑TGGTGTTGAGCAGTaaa                                                                                                                                                                                                                                   <  2:166215/251‑1 (MQ=255)          aaTCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGG‑TGTTGTTGAGCAGTaaaa                                                                                                                                                                                                                                  <  2:117182/251‑1 (MQ=255)             cGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGG‑TGGTGTTGAGCAGTAAAaag                                                                                                                                                                                                                                <  2:166012/250‑1 (MQ=255)              gACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGG‑TGTTGTTGAGCAGTAAAaaga                                                                                                                                                                                                                               >  2:374877/1‑250 (MQ=255)                         ggCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCa                                                                                                                                                                                                                   <  2:321321/250‑1 (MQ=255)                               agCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACaa                                                                                                                                                                                                             <  1:174333/250‑1 (MQ=255)                                                  ggACGTTTCAGGGCGCGCAGCGCGACCACTGTTAAGCGCGACCAGCCTCCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCTCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGG‑TGTTGTGGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCAGCATGGCGAGGAt                                                                                                                                                                                          <  2:270638/251‑1 (MQ=255)                                                     cGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGAT‑Ca                                                                                                                                                                                       >  2:269170/1‑250 (MQ=255)                                                                 cgcAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGAT‑CAACATGCCGCTCAt                                                                                                                                                                          >  2:33571/1‑251 (MQ=255)                                                                        cgaccacTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGAT‑CAACATGCCGCTCATCTCCa                                                                                                                                                                     <  2:308006/247‑1 (MQ=255)                                                                           ccacTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGAT‑CAACATGCCGCTCATCTCCAGcgcc                                                                                                                                                                >  2:309716/1‑248 (MQ=255)                                                                              cTTTTAAGCGCGACCCGCCTTCAATGCCCAGCGACTGACACAAAATACTGTAGAGGGCGGTGATATCGCGCATCGGGTTTTCAAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATACCGTCAACAGATTGCGGCAGGCCAATGG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACCATCACCGGCATGGCGAGGAT‑CAACATGCCGCTCATCTCCAGCGCCTg                                                                                                                                                              >  1:182538/1‑250 (MQ=255)                                                                                                    aatgcccAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGAT‑CAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCcgcg                                                                                                                                       >  1:336400/5‑251 (MQ=255)                                                                                                    aatgcccAGCGACTGACACAACATACTGTAGCGGGCGGAGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGAT‑CAACATGCCGCGCATCACCAGCGCCTGACCCGCCAGCCTTTTCTGCcgcg                                                                                                                                       >  1:356498/5‑251 (MQ=255)                                                                                                          cAGCGACTGAACCAACATACTGGAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGCCAATCACAATGCCGTCCGCAGATTGCGGCAGGCCAATGG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGGAGCCAGCACACTCCCCGGCAAGGCGAGGAT‑CAACATGCCGCTCATCTCCAGCGCCCGACCCGCCAGCCTTTTCTGCCGCGCCCgac                                                                                                                                 >  2:240693/1‑249 (MQ=255)                                                                                                              gACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGAT‑CAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTc                                                                                                                             >  1:262059/1‑251 (MQ=255)                                                                                                                                     ggcggTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGAT‑CAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGcatc                                                                                                       >  2:124723/1‑250 (MQ=255)                                                                                                                                       cggTGATATCGCGCGTCGGGTTCTCCAGTACTTTCAGAGCATAAGGGATCGCCATTAAAGCATTGGTGAAAATCAAACTGCCGTCAGCATATTGCGGCAGGCCAATGG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGACAGCACAATCCCCGGCATGGCGAGGATACACCA‑GCCGCTCATCTCCAGAGCCTGACCCGCCAGCATTTTCTTCCGCGCCCGAAGTTCAAGACTGCGCCATCGCCGCATCATg                                                                                                    >  2:243883/1‑251 (MQ=255)                                                                                                                                          tGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATACCGTCAGCAGATTGCGGCAGGCCAATGG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATACCCGGCATGGCGAGGAT‑CAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTc                                                                                                 >  2:185244/1‑251 (MQ=255)                                                                                                                                            atatCGCCCATCGGGTTTGCGAGTACTTGCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGACAGCAGATTGCGGCAGCCCAAGGG‑TGTTGTTGACAAGAAAAAAAAAGCCGTTAGCCAGCACAACCCCCGGCTTGGCGAGGAT‑CAACATGCCGCGCATCTCCAGCCCCTGACCCGCCATCATTTGCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAg                                                                                               <  1:32135/251‑1 (MQ=255)                                                                                                                                                   gcATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGAT‑CAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTAcc                                                                                        >  2:230326/1‑250 (MQ=255)                                                                                                                                                      tCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGAT‑CAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGcc                                                                                                                                          >  1:109276/1‑198 (MQ=255)                                                                                                                                                       cGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTACCGCATTGGTGAACATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGCT‑AAACATGCCGCTCCTCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCCGCAGAAGTTCGCGACTGCTCCCTAGCAGCATCATGGACAGCACAACGccc                                                                                     >  2:176587/1‑248 (MQ=255)                                                                                                                                                        gggTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGAT‑CAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGcaca                                                                                    >  1:191797/1‑250 (MQ=255)                                                                                                                                                            tttCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGAT‑CAACATGCCGCTCATCTCCAGCGCCTGACCCGcc                                                                                                                                                       <  1:23811/179‑1 (MQ=255)                                                                                                                                                            tttCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGAT‑CAACATGCCGCTCATCTCCAGCGCCTGACCCGcc                                                                                                                                                       >  2:23811/1‑179 (MQ=255)                                                                                                                                                                    tcTTTAAGCGCAAAGGGGACCGCCATTAACGCATTGGTAAAAAACACAATGCCGTCAGCAGATTGCGGCAGGC‑AATGGGTTTTGTTGAGCAGAAAAAAGAAGCCGGAAGCCAGCACAATCCCCGGCATGGCGAGGAT‑CAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTgccg                                                                        <  1:195088/249‑1 (MQ=255)                                                                                                                                                                             gcATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGAT‑CAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAAt                                                              >  2:347080/1‑251 (MQ=255)                                                                                                                                                                              cATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGAT‑CAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCCGCGCAAt                                                              >  2:65863/1‑250 (MQ=255)                                                                                                                                                                                 aaGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGTGGCAGGCCAATGG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGACAGCACAATCCCCGGCATGGCGAGGAT‑CAACATGCCGCTCATCTCCAGCGCCTGCCCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGCCTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCCGCGCAATACGc                                                          >  2:22228/1‑251 (MQ=255)                                                                                                                                                                                 aaGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATgg                                                                                                                                                                                                 <  2:158285/117‑1 (MQ=255)                                                                                                                                                                                 aaGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATgg                                                                                                                                                                                                 >  1:158285/1‑117 (MQ=255)                                                                                                                                                                                             ttAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGAT‑CAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCcac                                              >  2:358699/1‑251 (MQ=255)                                                                                                                                                                                               aaCGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGAT‑CAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCACCGAGGTCcac                                              >  2:214889/1‑249 (MQ=255)                                                                                                                                                                                                  gCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGAT‑CAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAgcgc                                         >  2:168279/1‑251 (MQ=255)                                                                                                                                                                                                     ttGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGAT‑CAACATGCCGCTCATCTCCAGCGCCt                                                                                                                                                               >  1:5255/1‑130 (MQ=255)                                                                                                                                                                                                     ttGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGAT‑CAACATGCCGCTCATCTCCAGCGCCt                                                                                                                                                               <  2:5255/130‑1 (MQ=255)                                                                                                                                                                                                      tGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGAT‑CAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGc                                     >  1:40136/1‑251 (MQ=255)                                                                                                                                                                                                           aaaaTCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGG‑GGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGAT‑CAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCCTAGCAGCATCATGGTCCGCACTACACACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCcacac                                >  2:139360/1‑250 (MQ=255)                                                                                                                                                                                                              aTCACAATGCCGTCAGCAGATTGCGGCAGGCCCATGG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGAT‑CAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCCATACGCAACGAGGTCCACAGCGCCTGCCACAGCAc                             >  2:116534/1‑251 (MQ=255)                                                                                                                                                                                                                     tGCCGTCAGCAGATTGCGGCAGGCCAATGG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGAT‑CAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTtgt                      >  1:194401/1‑251 (MQ=255)                                                                                                                                                                                                                            agcagATTGCGGCAGGCCAATGG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGAT‑CAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGc                >  2:352094/1‑250 (MQ=255)                                                                                                                                                                                                                               agATTGCGGCAGGCCAATGG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGAT‑CAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGCCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGCGCCAGCACtt            >  2:266532/1‑251 (MQ=255)                                                                                                                                                                                                                                 aTTGCGGCAGGCCAATGG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGAT‑CAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTcc          >  1:242189/1‑251 (MQ=255)                                                                                                                                                                                                                                         aGGCCAATGG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGAT‑CAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTg  >  1:366765/1‑251 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     AAAATCACCAATCGACAGCACGCATGCAAAGGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCCACCACTTTTAAGCGTGACCAGCCTTCAATCCCCAGCGACTGACATAACATGCTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGCACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGTGGCAGGCCGATAG‑TGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGAT‑CAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTG  >  NC_000913/73091‑73573

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑