Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	73,208	T→C	L438L (TTA→TTG)	thiP ←	thiamine ABC transporter membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	73,208	0	T	C	100.0%	183.1 / NA	54	L438L (TTA→TTG)	thiP	thiamine ABC transporter membrane subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/20);  total (34/20)

GCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACAGCACGCATGCAAAGGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCCACCACTTTTAAGCGTGACCAGCCTTCAATCCCCAGCGACTGACATAACATGCTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGCACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGTGGCAGGCCGATAGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTC  >  NC_000913/72961‑73450                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   gcagAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAAc                                                                                                                                                                                                                                                 >  1:365809/1‑251 (MQ=255)        aCAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGt                                                                                                                                                                                                                                          <  1:355001/251‑1 (MQ=255)          agagTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCCATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGt                                                                                                                                                                                                                                          >  1:214758/1‑249 (MQ=255)                  agcagAATTAACGCGGTGACCGCGCCGTCCTGGCTTCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCCGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCACTGCCCAGCGACTGACCCAACCTACTGTAGCGGGCGGt                                                                                                                                                                                                                                >  2:264140/1‑251 (MQ=255)                  agcagAATTAACGCGGTGACCGCGCCGTCCTGGCTGCTATAGTAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCTTTACCGACCAACTCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTGCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGCAgcgggcagt                                                                                                                                                                                                                                <  2:315965/251‑4 (MQ=255)                  agcagAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCCGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACAAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAgcgggcggg                                                                                                                                                                                                                                >  1:188369/1‑250 (MQ=255)                    cagAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTg                                                                                                                                                                                                                               >  1:101522/1‑250 (MQ=255)                    cagAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGAAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTg                                                                                                                                                                                                                               >  1:39881/1‑250 (MQ=255)                                 gTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCgg                                                                                                                                                                                                                 >  1:185336/1‑251 (MQ=255)                                  tGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCggg                                                                                                                                                                                                                >  1:295797/1‑251 (MQ=255)                                              ccTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAgtac                                                                                                                                                                                                     <  1:354627/250‑4 (MQ=255)                                               cTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACtt                                                                                                                                                                                                   >  1:331302/1‑251 (MQ=255)                                                  gCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCa                                                                                                                                                                                                <  2:265623/251‑1 (MQ=255)                                                   cTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTc                                                                                                                                                                                                 >  2:64040/1‑249 (MQ=255)                                                          aGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATa                                                                                                                                                                                         >  1:309100/1‑250 (MQ=255)                                                                        tGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAgcgg                                                                                                                                                                                                                                     <  1:77122/192‑1 (MQ=255)                                                                        tGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAgcgg                                                                                                                                                                                                                                     >  2:77122/1‑192 (MQ=255)                                                                         ggcagagATAAAACGGCAGGGTGCGGAAATCAGCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAAc                                                                                                                                                                         <  2:150956/248‑1 (MQ=255)                                                                          gTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCCTAAGGGATCGCCATTAAcc                                                                                                                                                                        >  2:103707/1‑250 (MQ=255)                                                                             gagaTAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCAt                                                                                                                                                                     <  1:111346/251‑1 (MQ=255)                                                                              agaTAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCAtt                                                                                                                                                                    >  1:341432/1‑251 (MQ=255)                                                                              agaTAAAACGGCAGGGTGCGGAAATCATCGTGACCGAACAACGCCACCACGCAAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTTATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGCATCGTCATTAACGCact                                                                                                                                                                    <  2:362141/251‑3 (MQ=255)                                                                                   aaaCGGCAGGGTGCGGAAAGCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCGGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGa                                                                                                                                                               <  2:133993/251‑1 (MQ=255)                                                                                    aaCGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATcgc                                                                                                                                                                                                                        <  1:248453/193‑1 (MQ=255)                                                                                    aaCGGCAGGGGGCGGAAATCATCGTTACCGAACAACGCCCCCACGCCCAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCCGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATcgc                                                                                                                                                                                                                        >  2:248453/1‑193 (MQ=255)                                                                                       ggCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCAAGCCAAAATCACCAATCGCCAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAAt                                                                                                                                                           >  1:166012/1‑251 (MQ=255)                                                                                        gCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATc                                                                                                                                                          >  2:277722/1‑251 (MQ=255)                                                                                          aGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATcac                                                                                                                                                        <  1:264140/251‑1 (MQ=255)                                                                                              tGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAACAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATg                                                                                                                                                    >  1:154546/1‑251 (MQ=255)                                                                                                 ggAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGATCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCg                                                                                                                                                 >  2:256612/1‑251 (MQ=255)                                                                                                 ggAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCg                                                                                                                                                 >  1:281759/1‑251 (MQ=255)                                                                                                  gAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGt                                                                                                                                                <  2:331302/251‑1 (MQ=255)                                                                                                                  aacaacGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGc                                                                                                                                  >  1:353100/1‑249 (MQ=255)                                                                                                                   acaacGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAgg                                                                                                                               >  1:19180/1‑251 (MQ=255)                                                                                                                   acaacCCCACCATCCCAGAATCACCAATCGACAACACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCAATTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAAAATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATtgcgaccgg                                                                                                                               >  2:359611/1‑244 (MQ=255)                                                                                                                        gCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGccaat                                                                                                                          >  2:221812/1‑248 (MQ=255)                                                                                                                                   aaaaTCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGttgttg                                                                                                                >  1:345307/1‑250 (MQ=255)                                                                                                                                    aaaTCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCTCTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCATGCCAATGGTGTTGTTGAg                                                                                                              <  2:326340/251‑1 (MQ=255)                                                                                                                                           cAATCGACAGAACGCAGGAAAAAGCCAGCGCCTGCGCCAGTGGACGTGTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTTACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGTTGGTGTTGAGCAGTaaa                                                                                                       <  2:166215/251‑1 (MQ=255)                                                                                                                                            aaTCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTaaaa                                                                                                      <  2:117182/251‑1 (MQ=255)                                                                                                                                               cGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGGTGTTGAGCAGTAAAaag                                                                                                    <  2:166012/250‑1 (MQ=255)                                                                                                                                                gACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAaaga                                                                                                   >  2:374877/1‑250 (MQ=255)                                                                                                                                                           ggCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCa                                                                                       <  2:321321/250‑1 (MQ=255)                                                                                                                                                                 agCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACaa                                                                                 <  1:174333/250‑1 (MQ=255)                                                                                                                                                                                    ggACGTTTCAGGGCGCGCAGCGCGACCACTGTTAAGCGCGACCAGCCTCCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCTCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTGGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCAGCATGGCGAGGAt                                                              <  2:270638/251‑1 (MQ=255)                                                                                                                                                                                       cGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCa                                                            >  2:269170/1‑250 (MQ=255)                                                                                                                                                                                                   cgcAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCAt                                               >  2:33571/1‑251 (MQ=255)                                                                                                                                                                                                          cgaccacTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCa                                          <  2:308006/247‑1 (MQ=255)                                                                                                                                                                                                             ccacTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGcgcc                                     >  2:309716/1‑248 (MQ=255)                                                                                                                                                                                                                cTTTTAAGCGCGACCCGCCTTCAATGCCCAGCGACTGACACAAAATACTGTAGAGGGCGGTGATATCGCGCATCGGGTTTTCAAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATACCGTCAACAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACCATCACCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTg                                   >  1:182538/1‑250 (MQ=255)                                                                                                                                                                                                                                      aatgcccAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCcgcg            >  1:336400/5‑251 (MQ=255)                                                                                                                                                                                                                                      aatgcccAGCGACTGACACAACATACTGTAGCGGGCGGAGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCGCATCACCAGCGCCTGACCCGCCAGCCTTTTCTGCcgcg            >  1:356498/5‑251 (MQ=255)                                                                                                                                                                                                                                            cAGCGACTGAACCAACATACTGGAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGCCAATCACAATGCCGTCCGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGGAGCCAGCACACTCCCCGGCAAGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCCGACCCGCCAGCCTTTTCTGCCGCGCCCgac      >  2:240693/1‑249 (MQ=255)                                                                                                                                                                                                                                                gACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTc  >  1:262059/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   GCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACAGCACGCATGCAAAGGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCCACCACTTTTAAGCGTGACCAGCCTTCAATCCCCAGCGACTGACATAACATGCTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGCACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGTGGCAGGCCGATAGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTC  >  NC_000913/72961‑73450

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑