Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	70,434	C→T	N16N (AAC→AAT)	araC →	DNA‑binding transcriptional dual regulator AraC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	70,434	0	C	T	100.0%	64.1 / NA	20	N16N (AAC→AAT)	araC	DNA‑binding transcriptional dual regulator AraC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/8);  total (12/8)

CACTTTTGTTACGCGTTTTTGTCATGGCTTTGGTCCCGCTTTGTTACAGAATGCTTTTAATAAGCGGGGTTACCGGTTGGGTTAGCGAGAAGAGCCAGTAAAAGACGCAGTGACGGCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGAATGGTATCACCAGTGGGTTTACT  >  NC_000913/70211‑70678                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                     cacTTTTGTTACGCGTGTTTGTCATGGCTTTGGTCCCGCTTTGTTACAGAATGCTTTTAATAAGCGGGGTTACCGGTGTGGTTAGCGAGAAGAGCCAGTAAAAGACGCAGTGACGGCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGcca                                                                                                                                                                                                                           <  2:200581/251‑2 (MQ=255)    ttttGTTACGCGTTTTTGTCATGGCTTTGGTCCCGCTTTGTTACAGAATGCTTTTAATAAGCGGGGTTACCGGTTTGGTTAGCGAGAAGAGCCAGTAAAAGACGCAGTGACGGCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGAtt                                                                                                                                                                                                                        >  1:322271/1‑251 (MQ=255)           aCGCGTTTTTGTCATGGCTTTGGTCCCGCTTTGTTACAGAATGCTTTTAATAAGCGGGGTTACCGGTTTGGTTAGCGAGAAGAGCCAGTAAAAGACGCAGTGACGGCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGcc                                                                                                                                                                                                                  <  1:42185/250‑1 (MQ=255)                        aTGGCTTTGGTCCCGCTTTGTTACAGAATGCTTTTAATAAGCGGGGTTACCGGTTTGGTTAGCGAGAAGAGCCAGTAAAAGACGCAGTGACGGCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGGTATCTCgc                                                                                                                                                                                                    >  2:160335/1‑250 (MQ=255)                         tGGCTTTGGTCCCGCTTTGTTACAGAATGCTTTTAATAAGCGGGGTTACCGGTTTGGTTAGCGAGAAGAGCCAGTAAAAGACGCAGTGACGGCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGGTTAATGCCCATCTGGTGGCGGGTTTTAAGCCCATTTAGGTCAACCGTTATCTCgct                                                                                                                                                                                                   >  2:125807/1‑249 (MQ=255)                         tGGCTTTGGTCCCGCTTTGATACAGAATGCTTTTAATAAGCGGGGTTACCGGTTTGGTTAGCGCGAAGAGCCAGTAAAAGACGCAGTGACGGCCATGTCTGATGCAATATGGACACTTGGTTTCTTCTCTGAATGGCGGGGGTATGAAAAGTATGGCTGAAGCGCAAAATGCTCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGGTTGAgggcccccgttttctccct                                                                                                                                                                                                   >  2:319498/1‑234 (MQ=255)                                                            ataaGCGGGGTTACCGGTTTGGTTAGCGAGAAGAGCCAGTAAAAGACGCAGTGACGGCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTta                                                                                                                                                                <  1:193000/251‑1 (MQ=255)                                                                    ggTTACCGGTTTGGTTAGCGAGAAGAGCCAGTAAAAGACGCAGTGACGGCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGGTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGGATGAAAGGGTATAttttc                                                                                                                                                         >  1:21733/1‑247 (MQ=255)                                                                        aCCGGTTTGGTTAGCGAGAAGAGCCAGTAAAAGACGCAGTGACGGCAATGTCTGATGCAATATGGACAAGTGGTTTCTTCTCTGAATGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGTCCTCTGGGAATGAAAGGTTATATGCTCAAtc                                                                                                                                                     <  2:218291/250‑1 (MQ=255)                                                                                  ttAGCGAGAAGAGCCAGTAAAAGACGCAGTGACGGCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGGTTAAAGCCGATTGAGGGCAACGGGTATCTCGATTTTTTTATCGACCGACCGCTGGGGATGAAAAGGTATATTCTCAATATCCACATTcgc                                                                                                                                          >  2:110868/1‑251 (MQ=255)                                                                                                                 aCGGCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTTACGCCGATTGCGGCCCACGGGTATCTCGATTTTTTTATCGACCGACCGCTGGGGATGAAAGGGTATATTCTCAATCTCACCATTCCAGGGGCGGGGGTGGTGGAAAAACAGGGACg                                                                                                             >  2:8067/1‑249 (MQ=255)                                                                                                                             gATGCAATATGGACAATTGGTTTCTTCTCTGAATGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTTTCGACCGACCGCTGGGAATTAAAGGTTATATTCTTAATCTCACCATTCGCGGTCCGGGGGTGGTGAAAAATCAGGGTCGAGAAttttttttcc                                                                                               >  1:268320/1‑244 (MQ=255)                                                                                                                                gCAATATGGACAATTGGTTTCTTCTCTGAATGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGAc                                                                                            <  1:311188/251‑1 (MQ=255)                                                                                                                                      tGGACAATTGGTTTCTTCTCTGAATGGCGGGAGTATGAAAAGTATGGCTGAAGAGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGGAAGAAAGGTTATATTTTCAATCTCACCATTCGCGGGTAGGGGGGGGGGAAAAATCAGGGACGAGAATTTGTTTTCCGACCggggg                                                                                      >  1:194830/1‑249 (MQ=255)                                                                                                                                                   tcttctCTGAATGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGt                                                                          <  2:21733/250‑1 (MQ=255)                                                                                                                                                           gAATGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAAtctc                                                                                                                                                   >  1:9436/1‑169 (MQ=255)                                                                                                                                                           gAATGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAAtctc                                                                                                                                                   <  2:9436/169‑1 (MQ=255)                                                                                                                                                                            aaaGTATGGCTGAAGCGCAAAATGTTCCCCTGCGGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACGGCTGGGAATGAAAGGTTATATTCTCAATCTCCCCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTGTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTAc                                                <  2:241955/251‑1 (MQ=255)                                                                                                                                                                                                                 gggATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGAATGGTATCACCAGt           >  1:365250/1‑251 (MQ=255)                                                                                                                                                                                                                          gTTTAATGCACATCTGGGGGCCGGTTTAACGCCAATTGAGGCCAACGGTTACCTCGATTTTTTTATCCACACAAAGCTGGGCATGAAAGGTTATATTCTCAATCTAACCATTCGCGGCCAGGGGGTGGTGAAAACTCAGGGACGAGAATTTTTTTGCCGCCCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCCCTACGGTCGTCATCCTGAGGCTAGCGAATGGTATCACCAGTGGGTTTACt  >  2:244974/1‑251 (MQ=255)                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                     CACTTTTGTTACGCGTTTTTGTCATGGCTTTGGTCCCGCTTTGTTACAGAATGCTTTTAATAAGCGGGGTTACCGGTTGGGTTAGCGAGAAGAGCCAGTAAAAGACGCAGTGACGGCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGAATGGTATCACCAGTGGGTTTACT  >  NC_000913/70211‑70678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑