Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	69,935	C→T	Q38Q (CAG→CAA)	araB ←	ribulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	69,935	0	C	T	100.0%	117.2 / NA	34	Q38Q (CAG→CAA)	araB	ribulokinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/23);  total (11/23)

CCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTTCCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTCTGCCAACGGGGATACCACTCTACGCTGGTGGCGATCTCTTCACCGGTAGCGCAGTCCACCGCCAAAGCTCGCACAGAATCACTGCCAAAATCGAGGCCAATTGCAATCGCCATCGTTTCACTCCATCCAAAAAAACGGGTATGGAGAAACAGTAGAGAGTTGCGATAAAAAGCGTCAGGTAGGATCCGCTAATCTTATGGATAAAAATGCTATGGCATAGCAAAGTG  >  NC_000913/69692‑70162                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                    ccACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTTTGCCAAc                                                                                                                                                                                                                              <  1:291035/251‑1 (MQ=255)  cacaATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTTTGCCAAc                                                                                                                                                                                                                              >  1:200882/1‑250 (MQ=255)  cacaATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTTTGCCAACg                                                                                                                                                                                                                             <  1:59631/251‑1 (MQ=255)  cacaATACGAACATCGCGTTCGGGTTTTCGGCAAACGCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTTTGCCAAc                                                                                                                                                                                                                              <  2:200882/250‑1 (MQ=255)     aaTACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACGCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTTTGCCAACgggg                                                                                                                                                                                                                          <  1:56513/251‑1 (MQ=255)              aTCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTTTGCCAACGGGGATACCActc                                                                                                                                                                                                                 >  1:218291/1‑251 (MQ=255)                 gctttCGGGTTTTCGGCAAACGCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAAGCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTTTGCCAACGGGGATACCACTCTAc                                                                                                                                                                                                              <  2:270804/248‑1 (MQ=255)                     tCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCATCACGTTACCTTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGTTTTTCAGTGCCGCGTCCATTGACTCAATGTAGTCACGCGTATGATGACGGAACTGGTTATTCGGGGCTTCACAAAATTGCCCTTTTTGCCAACGGGGATACCACTCTACGCt                                                                                                                                                                                                           <  1:360557/250‑1 (MQ=255)                      cGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTTTGCCAACGGGGATACCACTCTACGCtgg                                                                                                                                                                                                         >  1:112739/1‑251 (MQ=255)                             tCGGCAAACTCCGGGCGCAGCGCCAGCCCGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTGCAGTGCCGCTTCCATTTACTCAATGTAGTCACGCGGATGATGACGGAACTTGTTATTCGGGGCAGCACAAAATTGCCCTGTTTGCCAACGGGGATACCACTCGACGCTGGGGGCGa                                                                                                                                                                                                   <  2:212250/250‑1 (MQ=255)                                        cGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTTTGCCAACGGGGATACCACTCTACGCTGGTGGCGATCTCTTCACCgc                                                                                                                                                                                       >  1:61623/1‑250 (MQ=255)                                         gggCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTTTGCCAACGGGGATACCACTCTACGCTGGTGGCGATCTCTTCACCgct                                                                                                                                                                                      <  1:17823/251‑3 (MQ=255)                                                    atgcctgttcccggcGGCATCAATCGGAGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTTTGCCAACGGGGATACCACTCTACGCTGGTGGCGATCTCTTCACCGCTGGCGCAATCCa                                                                                                                                                                           <  2:160251/237‑1 (MQ=255)                                                                     cATCAATCGGGGAGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTCTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCATCTCAAAATTGCCCTTTTTGCCAACGGGGATACCACTCTACGCTGGTGGCGATCTCTTCACCGCTGGCGCAGTCCACCGCCAAAGCTCGcaca                                                                                                                                                          <  2:192789/251‑1 (MQ=255)                                                                                      gTCGAGCTGGTACTGTCAACGCCAATCCCGACCGCAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACAGGTTATTCGGGGCATCACAAAATTGCCCTTTTTGCCAACGGGGATACCACTCTACGCTGGTGGCGATCTCTTCACCGCTGGCGCAGTCCACCGCCAAAGCTCGCACAGAATCACTGCCAAAATc                                                                                                                                         <  2:259799/251‑1 (MQ=255)                                                                                      gTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTTTGCCAACGGGGATACCACTCTACGCTGGTGGCGATCTCTTCACCGCTGGCGCAGTCCACCGCCAAAGCTCGCACAGAATCACTGCCAAAATc                                                                                                                                         >  2:143514/1‑251 (MQ=255)                                                                                           gCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTTTGCCAACGGGGATACCACTCTACGCTGGTGGCGATCTCTTCACCGCTGGCGCAGTCCACCGCCAAAGCTCGCACAGAATCACTGCCAAAATCGAGGc                                                                                                                                    <  1:375419/251‑1 (MQ=255)                                                                                             cGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTTTGCCAACGGGGATACCACTCTACGCTGGTGGCGATCTCTTCACCGCTGGCGCAGTCCACCGCCAAAGCTCGCACAGAATCACTGCCAAAATCGAGGCCa                                                                                                                                  <  1:89625/251‑1 (MQ=255)                                                                                                       aaCGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTTTGCCAACGGGGATACCACTCTACGCTGGTGGCGATCTCTTCACCGCTGGCGCAGTCCACCGCCAAAGCTCGCACAGAATCACTGCCAAAATCGAGGCCAATTGCAATCg                                                                                                                        >  1:241955/1‑251 (MQ=255)                                                                                                         cGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTTTGCCAACGGGGATACCACTCTACGCTGGTGGCGATCTCTTCACCGCTGGCGCAGTCCACCGCCAAAGCTCGCACAGAATCACTGCCAAAATCGAGGCCAATTGCAATCGcc                                                                                                                      <  1:315005/251‑1 (MQ=255)                                                                                                                cccGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTTTGCCAACGGGGATACCACTCTACGCTGGTGGCGATCTCTTCACCGCTGGCGCAGTCCACCGCCAAAGCTCGCACAGAATCACTGCCAAAATCGAGGCCAATTGCAATCGCCATCGttt                                                                                                               <  2:210124/251‑1 (MQ=255)                                                                                                                                                     gCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACGGGTTATTCGGGGCATCACAAAATTGCCCTTTTTGCCAACGGGGATACCACTCTACGCTGGTGGCGATCTCTTCACCGCTGGCGCAGTCCACCGCCAAAGCTCGCACAGAATCACTGCCAAAATCGAGGCCAATTGCAATCGCCATCGTTTCACTCCATCCAAAAAAACGGGTATGGAGAAACAg                                                                             <  2:94501/248‑1 (MQ=255)                                                                                                                                                        aGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTTTGCCAACGGGGATACCACTCTACGCTGGTGGCGATCTCTTCACCGCTGGCGCAGTCCACCGCCAAAGCTCGCACAGAATCACTGCCAAAATCGAGGCCAATTGCAATCGCCATCGTTTCACTCCATCCAAAAAAACGGGTATGGAGAAACAGTag                                                                          >  1:267690/1‑248 (MQ=255)                                                                                                                                                           cgggTTTTCAGTGCCGCTTCCATTGACTCAATGTAGGCACGCTGATGATGACGGAGCTTGTTATGCGGAGCATCACAAACTTGCCTTTTTTGCCAACGGGGATACCACTCTACGCTGGTGGCGATCTCTTCACCGCTGTCGCGGGCCACCGCCACAGCTCGCGCAGAATCACTGCCAAAATCGAGTGCAAGTACAATCGACATCGTTTCACTCCATTCAAAAAAACGGTTATGGAGAATCAGTatatatta                                                                    <  2:121768/249‑8 (MQ=255)                                                                                                                                                                       gCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTGTTGCCAACGGGGATACCACTCTACGCTGGTGGCGATCTCTTCACCGCTGGCGCAGTCCACCGCCAAAGCTCGCACAGAATCACTGCCAAAATCGAGGCCAATTGCAATCGCCATCTTTTCACTCCATCCAAAAAAACGGTTATGGAGAAACAGGAGATAGTTGCGATAAAAAGc                                                        <  2:45942/251‑1 (MQ=255)                                                                                                                                                                                  ttaCTCAATGTATTCACGCGGATGATGACGGAACTGTTTATTCGGGGCATCACAAAATTGCCCTTTTTGCCAACGGGTATACCACTCTCCGCTGGTGGCGATCTCTTCACCGCTGGCGCAGTCCACCGCCAAAGCTCGCACAGAATCACTGCCAAAATCGAGGCCAATTGCAATCGCCATCGTTTCACGCCATCCAAAAAAACGGGTATGGAGAAACAGTCGAGAGTTTCGATAAAAAGCGTCAGGTAgg                                              <  2:192881/248‑1 (MQ=255)                                                                                                                                                                                                     ggATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTTTGCCAACGGGGATACCACTCTACGCTGGTGGCGATCTCTTCACCGCTGGCGCAGTCCACCGCCAAAGCTCGCACAGAATCACTGCCAAAATCGAGGCCAATTGCAATCGCCATCGTTTc                                                                                                              <  2:142207/167‑1 (MQ=255)                                                                                                                                                                                                     ggATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTTTGCCAACGGGGATACCACTCTACGCTGGTGGCGATCTCTTCACCGCTGGCGCAGTCCACCGCCAAAGCTCGCACAGAATCACTGCCAAAATCGAGGCCAATTGCAATCGCCATCGTTTc                                                                                                              >  1:142207/1‑167 (MQ=255)                                                                                                                                                                                                         gatgaCGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTTTGCCAACGGGGATACCACTCTACGCTGGTGGCGATCTCTTCACCGCTGGCGCAGTCCACCGCCAAAGCTCGCACAGAATCACTGCCAAAATCGAGGCCAATTGCAATCGCCATCGTTTCACTCCATCCAAAAAAACGGGTATGGAGAAACAGTAGAGAGTTGCGATAAAAAGCGTCAGGTAGGATCCGCTAATCTTATGGATaaaaa                      <  1:238148/251‑1 (MQ=255)                                                                                                                                                                                                            gaCGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTTTGCCAACGGGGACACCACTCTACGCTGGTGGCGATCTCTTCACCGCTGGCGCAGTCCACCGCCAAAGCTCGCACAGAATCACTGCCAAAATCGAGGCCACTTGCAATCGCCATCGTTTCACTCCATCCAAAAAAACGGGTATGGAGCAACAGTAGAGAGTTGCGATAAAAAGCGTCAGGTAGGATCCGCTAATCTTATGGATAAAAATGc                   >  1:183673/1‑251 (MQ=255)                                                                                                                                                                                                                       ttATTCGGGGCATCACAAAATTGCCCTTTTTGCCAACGGGGATACCACTCTACGCTGGTGGCGATCTCTTCACCGCTGGCGCAGTCCACCGCCAAAGCTCGCACAGAATCACTGCCAAAATCGAGGCCAATTGCAATCGCCATCGTTTCACTCCATCCAAAAAAACGGGTATGGAGAAACAGTAGAGAGTTGCGATAAAAAGCGTCAGGTAGGATCCGCTAATCTTATGGATAAAAATGCTATGGCATAGc        >  2:313489/1‑251 (MQ=255)                                                                                                                                                                                                                             ggggCATCACAAAATTGCCCTTTTTGCCAACGGGGATACCACTCTACGCTGGTGGCGATCTCTTCACCGCTGGCGCAGTCCACCGCCAAAGCTCGCACAGAATCACTGCCAAAATCGAGGCCAATTGCAATCGCCATCGTTTCACTCCATCCAAAAAAACGGGTATGGAGAAACAGTAGAGAGTTGCGATAAAAAGCGTCAGGTAGGATCCGCTAATCTTATGGATAAAAATGCTATGGCATAGCAAAgtg  <  2:164719/251‑1 (MQ=255)                                                                                                                                                                                                                                         aaTTGCCCTTTTTGCCAACGGGGATACCACTCTACGCTGGTGGCGATCTCTTCACCGCTGGCGCAGTCCa                                                                                                                                                                           <  1:313826/70‑1 (MQ=255)                                                                                                                                                                                                                                         aaTTGCCCTTTTTGCCAACGGGGATACCACTCTACGCTGGTGGCGATCTCTTCACCGCTGGCGCAGTCCa                                                                                                                                                                           >  2:313826/1‑70 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                    CCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTTCCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTCTGCCAACGGGGATACCACTCTACGCTGGTGGCGATCTCTTCACCGGTAGCGCAGTCCACCGCCAAAGCTCGCACAGAATCACTGCCAAAATCGAGGCCAATTGCAATCGCCATCGTTTCACTCCATCCAAAAAAACGGGTATGGAGAAACAGTAGAGAGTTGCGATAAAAAGCGTCAGGTAGGATCCGCTAATCTTATGGATAAAAATGCTATGGCATAGCAAAGTG  >  NC_000913/69692‑70162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑