Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 69,611 G→A Y146Y (TAC→TAT)  araB ← ribulokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091369,6110GA100.0% 98.6 / NA 29Y146Y (TAC→TAT) araBribulokinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/16);  total (13/16)

AGCTCATCAAAGAAACTGGCTGGCGGCAGGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTG‑CAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAA‑GCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTAGTCAACGTTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTCCGCTTCTT‑CAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTTCCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTT  >  NC_000913/69372‑69851
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                 
aGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTG‑CAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAA‑GCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGagt                                                                                                                                                                                                                                         >  1:259799/1‑250 (MQ=255)
          agaaccgggctggcGGCAAGCCGCCCCAGCTTTCGGGCCACAGAAATTTAGGCCCGGCGCTG‑AAACGCCCGCGACGAATACCCTGGGGGCGGGTGGTACCGGAAA‑GCAGAGCTGGCCCCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTg                                                                                                                                                                                                                              <  1:295241/244‑1 (MQ=255)
                   ggggcggcAAGCCCCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTG‑CAACGCCCGCGACGAATACCCTGCGGGCGGGTGGTACCGGAAA‑GCAGAGCTGGCCCCCAGTCGCACAGCTCAATCCACGAGGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCGGGAATAAAGACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGcgcg                                                                                                                                                                                                                     <  1:19767/249‑1 (MQ=255)
                         gcaagcccccccAGCTTTCGTGCCCAAGAGATTTATGCCCGGCGCTG‑CAACGTCCGCGACGAATATCCTGCGGGGGGGTGGTACCGGAAA‑GCAGAGCTGGCACCCAGTCGCACAGATCAACCCCCGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGACCCATTCGCGGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGcac                                                                                                                                                                                                               <  1:61165/243‑1 (MQ=255)
                              ccgccCCCGCTTTCGTGCCCCAGAGATTTATGCCCGGCGCTG‑CAACGTCCGCGACAAATATCCTGGGGGCGGGTGGTCCCGGAAA‑GCAGATCTGGCCCCCAGTCGCACAGCTCAATCCACGAGGGGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCGGGTTTTTGGCCCAGAACCTTTCGCTGGAATAAATACCGCCAAGATAGCGGGAGTAGTCAACATTGCCCGGTGCGTGGCACAAACg                                                                                                                                                                                                          <  1:300643/251‑1 (MQ=255)
                                gccCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTG‑CAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAA‑GCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACggg                                                                                                                                                                                                        >  1:314648/1‑251 (MQ=255)
                                      gctttgggcccagagagTTTTAGGCCCGGCG‑TGACAACGCCCGCGAGGAATACCCGGGGGGGGGGTG‑TTCCGGAAAAGCGGGGCTGGCCCCAATTCGCCCAGCTAAACCCCCGAGGCGGCAGATTGCGCCGCGGCGCTGTCGTGGCGAGCCACAGGCAGGATTTTTGCCAAGAACCATTGGCTGGAAAAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAAtct                                                                                                                                                                                                  <  2:289710/238‑1 (MQ=255)
                                      gCTTTCGTGCCACAGAGATTTAGGCCCGGCGCTG‑CAACGCCCGCGGCGAATATCCGGCGGGCGGGTGGT‑CCGGAAAAGCAGAGCTGGCACCCAGTCGCACAGCCCAATCCACGAGGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTGGCCCAGAACCATTCGCGGAAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAAtct                                                                                                                                                                                                  <  1:64655/251‑1 (MQ=255)
                                            gTGCCACAGAGATTTATGCCCGGCGCTG‑CAACGTCCGCGACGAATATACTGCGGGCGGGTGGTACCGGAAA‑GCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGCTTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCCCAAACGGGTAATCTCTTcgc                                                                                                                                                                                            >  2:375419/1‑249 (MQ=255)
                                                                               ccgggacgaATACCCTGGGGGGGGGGGGTACCGGAAA‑GCAGAGCTGGCACCCAGTCGCACAGCACAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCGGGCGAGTCACAGGCAGGATTTTGGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATAc                                                                                                                                                         <  2:76950/247‑1 (MQ=255)
                                                                                    acgaATATCCTGCGGGCGGGTGGTACCGGAAA‑GCAGAGCGGGCCCCCGGTCGCACACCTCAATCCACGATGCGGCAGATTGCCCCACGGCGCTGTCCGGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACa                                                                                                                                                    <  1:116612/251‑1 (MQ=255)
                                                                                     cgaATATCCTGCGGGCGGGTGGTACCGGCAA‑GCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑Gcttctttc                                                                                                                                                                                    >  2:312871/1‑216 (MQ=255)
                                                                                     cgaATACCCTGCGGGCGGGTGGTACCGGAAA‑GCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCGCGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATCCGCTGGAATAAATGCCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑Gcttctttc                                                                                                                                                                                    <  1:312871/218‑3 (MQ=255)
                                                                                          gtccggggggggggTGGTACCGGAAG‑GCGGGCCGGGCACCCAGTCGCCCAGCTCACTCCAGGGTGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGATTCACAGGCAGGATTTTTGCCCAGACCCATTCGCTGAAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATcgcg                                                                                                                                               <  2:136263/239‑1 (MQ=255)
                                                                                                                gAAA‑GCAGTGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCgg                                                                                                                        >  1:94501/1‑251 (MQ=255)
                                                                                                                 aaa‑GCAGAGCTAGCACCCAGTCGAACAGCTCAATCCACGATGCAGCAGAGTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATACATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCCACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAACCTCCggg                                                                                                                       >  2:46216/1‑251 (MQ=255)
                                                                                                                       agagCTGGCCCCCAGTCGCACAGCTCAACCCACGAGGCGGCAGATTGCGCCCCGGCGCTTTCCGGGCGAGTCACATGCAGGATTTTTGCCCAAAACCATTCGCTGGAAAAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAg                                                                                                                  <  1:47267/251‑1 (MQ=255)
                                                                                                                              gCACCAAGTCGCACAGCTAAATCAGCGAGGGGGCAGATTGCCCCAGGGAGCTGTCCGGGCGAGTAACAGGAAGGATTTTGGCCCAGAACCATGCGCGGAAAGAAATACCGCCAATATAGCGGGAGTAGTCAACATGGCCAGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGc                                                                                                           <  1:160565/251‑1 (MQ=255)
                                                                                                                                                                    ggcgcCACGGCGCTGTCCGGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAAAAAAGACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGcc                                                                     <  1:184439/250‑1 (MQ=255)
                                                                                                                                                                      cgcCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGtgtg                                                                                                                                                                        >  1:76696/1‑150 (MQ=255)
                                                                                                                                                                        ccACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGt                                                                  >  1:164719/1‑250 (MQ=255)
                                                                                                                                                                                       ggcgcttcactggcggattttttCCCCAGAACCATTCGTTGGAATAATTACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATc                                                  <  1:137360/233‑1 (MQ=255)
                                                                                                                                                                                                                ccAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCg                          >  1:45942/1‑250 (MQ=255)
                                                                                                                                                                                                                     aCCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCAGACCACAGCTGCGCGCTGTTCGACGCt                     >  2:291035/1‑250 (MQ=255)
                                                                                                                                                                                                                        aTTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGc                 >  2:17823/1‑251 (MQ=255)
                                                                                                                                                                                                                             cTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGc            >  2:88491/1‑251 (MQ=255)
                                                                                                                                                                                                                                       caccgccaATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGGTCGACGCGAAGCTCTGCAAGCACGGtt  <  1:271591/246‑1 (MQ=255)
                                                                                                                                                                                                                                         ccgccaATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAgc                                                                                                                 >  1:110624/4‑138 (MQ=255)
                                                                                                                                                                                                                                         ccgccaATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAgc                                                                                                                 <  2:110624/135‑1 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                 
AGCTCATCAAAGAAACTGGCTGGCGGCAGGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTG‑CAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAA‑GCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTAGTCAACGTTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTCCGCTTCTT‑CAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTTCCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTT  >  NC_000913/69372‑69851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑