Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	69,605	A→G	G148G (GGT→GGC)	araB ←	ribulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	69,605	0	A	G	100.0%	88.1 / NA	26	G148G (GGT→GGC)	araB	ribulokinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/14);  total (12/14)

AGCTCATCAAAGAAACTGGCTGGCGGCAGGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTG‑CAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAA‑GCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTAGTCAACGTTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTCCGCTTCTT‑CAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTTCCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGC  >  NC_000913/69372‑69841                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                              aGCTCATCAAAGAAACTGGCTGGCGGCAAGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTG‑CAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAA‑GCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGagt                                                                                                                                                                                                                               >  1:259799/1‑250 (MQ=255)           agaaccgggctggcGGCAAGCCGCCCCAGCTTTCGGGCCACAGAAATTTAGGCCCGGCGCTG‑AAACGCCCGCGACGAATACCCTGGGGGCGGGTGGTACCGGAAA‑GCAGAGCTGGCCCCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTg                                                                                                                                                                                                                    <  1:295241/244‑1 (MQ=255)                    ggggcggcAAGCCCCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTG‑CAACGCCCGCGACGAATACCCTGCGGGCGGGTGGTACCGGAAA‑GCAGAGCTGGCCCCCAGTCGCACAGCTCAATCCACGAGGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCGGGAATAAAGACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGcgcg                                                                                                                                                                                                           <  1:19767/249‑1 (MQ=255)                          gcaagcccccccAGCTTTCGTGCCCAAGAGATTTATGCCCGGCGCTG‑CAACGTCCGCGACGAATATCCTGCGGGGGGGTGGTACCGGAAA‑GCAGAGCTGGCACCCAGTCGCACAGATCAACCCCCGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGACCCATTCGCGGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGcac                                                                                                                                                                                                     <  1:61165/243‑1 (MQ=255)                               ccgccCCCGCTTTCGTGCCCCAGAGATTTATGCCCGGCGCTG‑CAACGTCCGCGACAAATATCCTGGGGGCGGGTGGTCCCGGAAA‑GCAGATCTGGCCCCCAGTCGCACAGCTCAATCCACGAGGGGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCGGGTTTTTGGCCCAGAACCTTTCGCTGGAATAAATACCGCCAAGATAGCGGGAGTAGTCAACATTGCCCGGTGCGTGGCACAAACg                                                                                                                                                                                                <  1:300643/251‑1 (MQ=255)                                 gccCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTG‑CAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAA‑GCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACggg                                                                                                                                                                                              >  1:314648/1‑251 (MQ=255)                                       gctttgggcccagagagTTTTAGGCCCGGCG‑TGACAACGCCCGCGAGGAATACCCGGGGGGGGGGTG‑TTCCGGAAAAGCGGGGCTGGCCCCAATTCGCCCAGCTAAACCCCCGAGGCGGCAGATTGCGCCGCGGCGCTGTCGTGGCGAGCCACAGGCAGGATTTTTGCCAAGAACCATTGGCTGGAAAAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAAtct                                                                                                                                                                                        <  2:289710/238‑1 (MQ=255)                                       gCTTTCGTGCCACAGAGATTTAGGCCCGGCGCTG‑CAACGCCCGCGGCGAATATCCGGCGGGCGGGTGGT‑CCGGAAAAGCAGAGCTGGCACCCAGTCGCACAGCCCAATCCACGAGGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTGGCCCAGAACCATTCGCGGAAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAAtct                                                                                                                                                                                        <  1:64655/251‑1 (MQ=255)                                             gTGCCACAGAGATTTATGCCCGGCGCTG‑CAACGTCCGCGACGAATATACTGCGGGCGGGTGGTACCGGAAA‑GCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGCTTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCCCAAACGGGTAATCTCTTcgc                                                                                                                                                                                  >  2:375419/1‑249 (MQ=255)                                                                                ccgggacgaATACCCTGGGGGGGGGGGGTACCGGAAA‑GCAGAGCTGGCACCCAGTCGCACAGCACAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCGGGCGAGTCACAGGCAGGATTTTGGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATAc                                                                                                                                               <  2:76950/247‑1 (MQ=255)                                                                                     acgaATATCCTGCGGGCGGGTGGTACCGGAAA‑GCAGAGCGGGCCCCCGGTCGCACACCTCAATCCACGATGCGGCAGATTGCCCCACGGCGCTGTCCGGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACa                                                                                                                                          <  1:116612/251‑1 (MQ=255)                                                                                      cgaATATCCTGCGGGCGGGTGGTACCGGCAA‑GCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑Gcttctttc                                                                                                                                                                          >  2:312871/1‑216 (MQ=255)                                                                                      cgaATACCCTGCGGGCGGGTGGTACCGGAAA‑GCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCGCGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATCCGCTGGAATAAATGCCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑Gcttctttc                                                                                                                                                                          <  1:312871/218‑3 (MQ=255)                                                                                           gtccggggggggggTGGTACCGGAAG‑GCGGGCCGGGCACCCAGTCGCCCAGCTCACTCCAGGGTGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGATTCACAGGCAGGATTTTTGCCCAGACCCATTCGCTGAAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATcgcg                                                                                                                                     <  2:136263/239‑1 (MQ=255)                                                                                                                 gAAA‑GCAGTGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCgg                                                                                                              >  1:94501/1‑251 (MQ=255)                                                                                                                  aaa‑GCAGAGCTAGCACCCAGTCGAACAGCTCAATCCACGATGCAGCAGAGTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATACATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCCACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAACCTCCggg                                                                                                             >  2:46216/1‑251 (MQ=255)                                                                                                                        agagCTGGCCCCCAGTCGCACAGCTCAACCCACGAGGCGGCAGATTGCGCCCCGGCGCTTTCCGGGCGAGTCACATGCAGGATTTTTGCCCAAAACCATTCGCTGGAAAAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAg                                                                                                        <  1:47267/251‑1 (MQ=255)                                                                                                                               gCACCAAGTCGCACAGCTAAATCAGCGAGGGGGCAGATTGCCCCAGGGAGCTGTCCGGGCGAGTAACAGGAAGGATTTTGGCCCAGAACCATGCGCGGAAAGAAATACCGCCAATATAGCGGGAGTAGTCAACATGGCCAGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGc                                                                                                 <  1:160565/251‑1 (MQ=255)                                                                                                                                                                     ggcgcCACGGCGCTGTCCGGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAAAAAAGACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGcc                                                           <  1:184439/250‑1 (MQ=255)                                                                                                                                                                       cgcCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGtgtg                                                                                                                                                              >  1:76696/1‑150 (MQ=255)                                                                                                                                                                         ccACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGt                                                        >  1:164719/1‑250 (MQ=255)                                                                                                                                                                                        ggcgcttcactggcggattttttCCCCAGAACCATTCGTTGGAATAATTACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATc                                        <  1:137360/233‑1 (MQ=255)                                                                                                                                                                                                                 ccAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCg                >  1:45942/1‑250 (MQ=255)                                                                                                                                                                                                                      aCCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCAGACCACAGCTGCGCGCTGTTCGACGCt           >  2:291035/1‑250 (MQ=255)                                                                                                                                                                                                                         aTTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGc       >  2:17823/1‑251 (MQ=255)                                                                                                                                                                                                                              cTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGc  >  2:88491/1‑251 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                              AGCTCATCAAAGAAACTGGCTGGCGGCAGGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTG‑CAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAA‑GCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTAGTCAACGTTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTCCGCTTCTT‑CAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTTCCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGC  >  NC_000913/69372‑69841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑