Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 443 T→C V36A (GTC→GCC)  thrA → fused aspartate kinase/homoserine dehydrogenase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134430TC95.7% 60.7 / ‑5.8 23V36A (GTC→GCC) thrAfused aspartate kinase/homoserine dehydrogenase 1
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (9/13);  total (9/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGACTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTA  >  NC_000913/198‑661
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                          
cATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGCCctct                                                                                                                                                                                                                       <  2:45099/251‑1 (MQ=255)
cATTAGCACCACCATTACCACCACCAGAACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGCCctct                                                                                                                                                                                                                       <  2:15669/251‑1 (MQ=255)
      caccacCATTACCACCACCACCACCATTACCACATGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGCCCTCTCTGccc                                                                                                                                                                                                                 <  1:43308/251‑1 (MQ=255)
                            cccatTCCCACAGGTACCGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTTTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCGGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGCCCTCTCTGCCCCCGCCAAAATCACCAACCACCt                                                                                                                                                                                           <  1:156050/250‑1 (MQ=255)
                                                                                 aGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGCCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATc                                                                                                                                       >  1:126338/1‑250 (MQ=255)
                                                                                    ccGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATTCAGAACGTTTTCTGCTTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGTGCAGGTGGCCACCGCCCTCGCTGCCCCCGCCAAAATCACCATCCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCg                                                                                                                                   <  1:163458/251‑1 (MQ=255)
                                                                                      gcccttggcAGTCCGGGATTTTTTTTTCGGCCAAAGGTACCGGGGGAACAACCATTCGAGTGTTGAAGTGCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGGGTGTTGCGGATATTCTGGAAAGCAATCCCAGGCAGGGGCAGGGGGGCGCCGCCCTCTCTCCCCCCGCCAGAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCGTTCGCCAATATCAGCGag                                                                                                                                 <  2:241437/243‑2 (MQ=255)
                                                                                             aCAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGCCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAAc                                                                                                                          <  1:213944/251‑1 (MQ=255)
                                                                                                               ttCGAACAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGGGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCGGGAAAGCAATGACAGGCAGGGGCAGGTGGCCACCGCCCTCTCGGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACtt                                                                                                         <  2:45630/250‑1 (MQ=255)
                                                                                                                                aGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGCCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGACTcgccgcc                                                                                       <  1:334362/251‑1 (MQ=255)
                                                                                                                                          cATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGCCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGACTCGCCGCCGCCCAGCCgg                                                                             >  1:241380/1‑251 (MQ=255)
                                                                                                                                              cGAGTGTTGAAGTGCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCGGGGGCAGGTGGCCACCGCCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTATCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGATCTTTTGCCGGGACTCGCCGCCGCCCAGCCGGGGtt                                                                         <  1:208268/251‑1 (MQ=255)
                                                                                                                                                                ggTACATCAGTGTCCAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAACGAAATGCCAGGCAGGGGCAGGTGGCCACCGCCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGCTGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCCATATCAGCGATGCCGAACGTCTTTTTGCCGAACTTTTGACGGGACTCGCCGCCGCCCAGCCCGGCTTCCCGCTGGCCCAATTGa                                                        >  2:138372/1‑250 (MQ=255)
                                                                                                                                                                          tGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGCCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGACTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTtcgtc                                             >  2:235351/1‑251 (MQ=255)
                                                                                                                                                                              aaaTGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGCCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGACTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTTCGTCGa                                           <  2:242371/249‑1 (MQ=255)
                                                                                                                                                                              aaaTGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGCCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGACTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTTCGTCGa                                           >  1:242371/1‑249 (MQ=255)
                                                                                                                                                                                  gCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAACGCAATGCCAGGCAGGGGCAGGTGGCCACCGCCCTCTCTTCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAACCCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTCTGCCGACCTTTTGACGGGACTCGCCGCCGCCCAGCCCGGGTTCCCGCTGGCGCAATTGATAACTTTCGTCGATCAGGa                                     <  1:46660/251‑1 (MQ=255)
                                                                                                                                                                                          ttttCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGCCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGACTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGccc                             >  2:343709/1‑251 (MQ=255)
                                                                                                                                                                                                  gtgtTGCCAATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGACTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATaaaa                     <  1:330038/251‑1 (MQ=255)
                                                                                                                                                                                                             atTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGCCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGACTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGc           >  1:12042/1‑250 (MQ=255)
                                                                                                                                                                                                                     aaGCAATGCCAGGCAGGGGCAGGTGGCCACCGCCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGACTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCAttt  >  2:92676/1‑250 (MQ=255)
                                                                                                                                                                                                                              caggcaggGGCAGGTGGCCACCGCCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGACTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTg            <  1:368269/232‑1 (MQ=255)
                                                                                                                                                                                                                              caggcaggGGCAGGTGGCCACCGCCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGACTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTg            >  2:368269/1‑232 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                          
CATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGACTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTA  >  NC_000913/198‑661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑