Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913891,0310CT38.5% 22.6 / 10.5 26S40F (TCT→TTT) ybjCDUF1418 domain‑containing protein YbjC
Reads supporting (aligned to +/- strand):  ref base C (5/11);  new base T (10/0);  total (15/11)
Fisher's exact test for biased strand distribution p-value = 7.23e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ATCACGCTAAAATGTATTCGCTGAAAGTAGGTTTAACCTGTTGCATTAATTGCTAAAAGCTATAACTGTTAAACACAATACAGTGAAAAGTTTTAGACTGAAGGCTCACTTTGCAGAGGGAAGCGTATGCGCGCGATCGGTAAATTGCCTAAAGGCGTGTTGATACTGGAATTTATCGGAATGATGCTACTGGCGGTGGCGCTGCTGTCGGTAAGCGACTCCCTGTCGCTGCC‑TG‑AGCCATTTTCTCGGCCAGAAGTGCAGATTCTGATGATTTTTCTCGGTG‑TTTTGCTCATGCTTCCCGCTGCGGTGGTGGTTATTCTT‑CAGGTGGCAAAACGTCTTG‑CCCCACAGCTGATGAACCGTCCACCGCAATATTCACGTTCAGAAAGAGAAAAAGATAATGACGCCAACCATTGAACTTATTTGTGGCCATCGCTCCATTCGCCATTTCACTGATGAACCCATTTC  >  NC_000913/890787‑891251
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                               
aTCACGCTAAAATGTATTCGCTGAAAGTAGGTTTAACCTGTTGCATTAATTGCTAAAAGCTATAACTGTTAAACACAATACAGTGAAAAGTTTTAGACTGAAGGCTCACTTTGCAGAGGGAAGCGTATGCGCGCGATCGGTAAATTGCCTAAAGGCGTGTTGATACTGGAATTTATCGGAATGATGCTACTGGCGGTGGCGCTGCTGTCGGTAAGCGACTCCCTGTCGCTGCC‑TG‑AGCCATTTTCTCGGcc                                                                                                                                                                                                                           <  1:284679/251‑1 (MQ=255)
                   gCTGAAAGTAGGTTTAACCTGTTGCATTAATTGCTAAAAGCTATAACTGTTAAACACAATACAGTGAAAAGTTTTAGACTGAAGGCTCACTTTGCAGAGGGAAGCGTATGCGCGCGATCGGTAAATTGCCTAAAGGCGTGTTGATACTGGAATTTATCGGAATGATGCTACTGGCGGTGGCGCCGGTGTCGGGAAAAGACCCCCTGTCGCCGCC‑CG‑AGCCCCTTTCTCCGCCAGAATTGTCGATTCtgatg                                                                                                                                                                                                        >  2:288928/1‑251 (MQ=255)
                          gTAGGTTTAACCTGTTGCATTAATTGCTAAAAGCTATAACTGTTAAACACAATACAGTGAAAAGTTTTAGACTGAAGGCTCACTTTGCAGAGGGAAGCGTATGCGCGCGATCGGTAAATTGCCTAAAGGCGTGTTGATACTGGAATTTATCGGAATGATGCTACTGGCGGAGGCGCTGCTGTTGGTTAGCGAATCCCTGTTGCTTCC‑TG‑TGGCAATTTTTTGGCCAGAAGGGCAGGTTCTTATGAtttttt                                                                                                                                                                                                 >  1:292424/1‑250 (MQ=255)
                                          gCATTAATTGCTAAAAGCTATAACTGTTAAACACAATACAGTGAAAAGTTTTAGACTGAAGGCTCACTTTGCAGAGGGAAGCGTATGCGCGCGATCGGTAAATTGCCTAAAGGCGTGTTGATACTGGAATTTAACGGAATGATGCTACTGGCGGTGGGGCTGCTGTCGGGAAGAGACTCCCTCACGCCGCC‑TG‑AGGCATTTTTTTGGCCAGAAGAGCAGAGTCTTATGGTTTTTTTCTGTG‑tttttttctt                                                                                                                                                                                >  2:333809/1‑245 (MQ=255)
                                                   gCTAAAAGCTATAACTGTTAAACACAATACAGTGAAAAGTTTTAGACTGAAGGCTCACTTTGCAGAGGGAAGCGTATGCGCGCGATCGGTAAATTGCCTAAAGGCGTGTTGATACTGGAATTTATCGGAATGATGCTACTGGCGGTGGCGCTGCTGTCGGTAAGCGACTCCCTGCCGCTGCC‑TG‑AGGCAGTTTCTCGGCCAGAAGTGCAGATTCTGATGATTTTTCTCGGTG‑TTTTGCTCATGCTTCCCGc                                                                                                                                                                       <  2:179017/251‑1 (MQ=255)
                                                     tAAAAGCTATAACTGTTAAACACAATACAGTGAAAAGTTTTAGACTGAAGGCTCACTTTGCAGAGGGAAGCGTATGCGCGCGATCGGTAAATTGCCTAAAGGCGTGTTGATACTGGAATTTATCGGAATGATGCTACTGGCGGTGGCGGTGCTGTCGGGAAGGGACGACCTGTCGCTGCC‑TG‑AGCCCTTTTTTCGGCCCGAAGAGCAAAAGTTGATGGTTTTTCTCTGTG‑TGTTGTTCAtagttcccccg                                                                                                                                                                      >  1:275007/1‑240 (MQ=255)
                                                             ataACTGTTAAACACAATACAGTGAAAAGTTTTAGACTGAAGGCTCACTTTGCAGAGGGAAGCGTATGCGCGCGATCGGTAAATTGCCTAAAGGCGTGTTGATACTGGAATTTATCGGAATGATGCTACTGGCGGTTGGGCTGCTGTCGGTAAACGACTCCCCGTCGCTGCCCTG‑‑GCCCTTTTTTCCGCCCGAAGGGGCGGTTCTGATGTTTTTTTTTGGGG‑TTTTTTTCCTGCTTCCCGCTGCggggggg                                                                                                                                                             >  2:194504/1‑246 (MQ=255)
                                                                                cAGTGAAAAGTTTTAGACTGAAGGCTCACTTTGCAGAGGGAAGCGTATGCGCGCGATCGGTAAATTGCCTAAAGGCGTGTTGATACTGGAATTTATCGGAATGATGCTACTGGCGGTGGCGCTGCTGTCGGTAAACGACTCCCCGTCGCTGCC‑CG‑AGGCATTTTTTCCGGCAGAAGTGCAGATTTTGATGATTTTTTTTGGTG‑TTTTTGTCATTCTTCCCCCTGCgggggggggttttttttcggtggga                                                                                                                                           >  1:188944/1‑227 (MQ=255)
                                                                                  gTGAAAAGTTTTAGACTGAAGGCTCACTTTGCAGTGGGAAGCGTATGCGCGCGATCGGTAAATTGCCTAAAGGCGTGTTGATACTGGAATTTATCGGAATGATGCTACTGGCGGTGGCGCTGCTTTCGGTAAGCGACTCCCTGTCGCCGCC‑TG‑TGGCATTTTCTCGGGCCGGAGGGCAGATTCTGATTATTTTTTTCGGGGGTTTTG‑TCATGGTTTCCGCCGCGGTGGGGGTTTTTTTT‑Caagggggaaa                                                                                                                                        >  2:38693/1‑241 (MQ=255)
                                                                                     aaaaGTTTTAGACTGAAGGCTCACTTTGCAGAGGGAAGCGTATGCGCGCGATCGGTAAATTGCCTAAAGGCGTGTTGATACTGGAATTTATCGGAATGATGCTACTGGCGGTGGCGCTGCTGTCGGTAAGCGACTCCCTGTCGCTGCC‑TG‑AGCCATTTTTTCGGCCCGAAGTGGCGGTTTTGATGATTTTTTTCGGGG‑TTTTTCTCATGCTTTCCGCTGCGGTGGTGGTTATTCTT‑CAGGTGGCaaaaatt                                                                                                                                    >  1:193109/1‑248 (MQ=255)
                                                                                                   gAAGGCTCACTTTGCAGAGTGAAGCGTATGCGCGCGATCGGTAAATTGCCTAAAGGCGTGTTGATACTTGAATTTATCGGAATGATGCTGCTGGCGGTGGCTCTGCTGTCGGTAAGCGACTCCCTGTCGCTGCC‑TG‑AGCCATTTTCTCGGCCAGAAGTGCAGATTCTGATGATTGTTCTCGGTG‑TTTTGCTCATGCTTCCCGCTGCGGTGGTGGTTATTCTT‑CAGGTGGCAAAACGTCTTG‑CCCCACAGCt                                                                                                                     <  2:375691/251‑1 (MQ=255)
                                                                                                   gAAGGCTCACTTTGCAGAGGGAAGCGTATGCGCGCGATCGGTAAATTGCCTAAAGGCGTGTTGATACTGGAATTTATCGGAATGATGCTACTGGCGGTGGCGCTGCTGTCGGTAAGCGACTCCCTGTCGCTGCC‑TG‑AGCCATTTTCTCGGCCAGAAGTGCAGATTCTGATGATTCTTCTCGGTG‑TTTTGCTCATGCTTCCCGCTGCGGTGGTGGTTATTCTT‑CAGGTGGCAAAACGTCTTG‑CCCCACAGCt                                                                                                                     <  2:188944/251‑1 (MQ=255)
                                                                                                          cACTTTGCAGAGGGAAGCGTATGCGCGCGATCGGTAAATTGCCGAAATGCGGGTTGATACTGGAATTTATCGGAATGATGCTACTGGCGGTGGCGCTGCTGTCGGTAAGCGACTCCCTGTCGCTGCC‑TG‑GGCCATTTTCTCGGCCAGAAGTGCAGATTCTGATGATTTTTCTCGGTG‑TTTTGCTCATGCTTCCCGCTGCGGTGGTGGTTATTCTT‑CAGGTGGCAAAACGTCTTG‑CCCGACAGCTGATGAAc                                                                                                              <  2:268228/251‑1 (MQ=255)
                                                                                                                         aGCGTATGCGCGCGATCGGTAAATTGCCTAAAGGCGTGTTGATACTGGAATTTATCGGAATGATGCTACTGGCGGTGGCGCTGCTGTCGGTAAGCGACTCCCTGTCGCTGCC‑TG‑AGCCATTTTCTCGGCCAGAAGTGCAGATTCTGATGATTTTTCTCGGTG‑TTTTGCTCATGCTTCCCGCTGCGGTGGTGGTTATTCTT‑CAGGTGGCAAAACGTCTTG‑CCCCACAGCTGATGAACCGTCCACCGCAatat                                                                                               <  1:55425/251‑1 (MQ=255)
                                                                                                                                   cgcgATCGGTAAATTGCCTAAAGGCGTGTTGATACTGGAATTTATCGGAATGATGCTACTGGCGGTGGCGCTGCTGTCGGTAAGCGACTCCCTGTCGCTGCC‑TG‑AGCCATTTTCTCGGCCAGAAGTGCAGATTCTGATGATTTTTCTCGGTG‑TTTTGCTCATGCTTCCCGCTGCGGTGGTGGTTATTCTT‑CAGGTGGCAAAACGTCTTG‑CCCCACAGCTGATGAACCGTCCACCGCAATATTCACGTTCAg                                                                                     <  1:64518/251‑1 (MQ=255)
                                                                                                                                             aaaTTGCCTAAAGGCGTGTTGATACTGGAATTTATCGGAATGATGCTACTGGCGGTGGCGCTGCCGTCGGTAAACGACTCCCTGTCGCTGGC‑TG‑AGCCCTTTTTTCGGCCAGAAGTGCAGATTCTGGTTGTTTTTTTCGGTG‑GTTTGTTCCTGCTTCCCGCTGCGGGGGGGGTTTTTTTT‑CAGGTGGCAAAACGTCTTG‑CCCCCCAGCTGATGAAACGGCCACCGCAATATTTACGTTCAGAAAGAGaaa                                                                            >  1:8369/1‑250 (MQ=255)
                                                                                                                                              aaTTGCCTAAAGGCGTGTTGATACTGGAATTTATCGGAATGATGCTACTGGCGGTGGCGCTGCTGTCGGTAAGCGACTCCCTGTCGCTGCC‑TG‑AGCCATTTTCTCGGCCCGAAGTGCAGATTTTGATGATTTTTTTCGGTG‑TTTTTCTCATGCTTCCCGCCGCGGGGGGGGTTTTTCTT‑CAGGTGGCAAAACGTCTTG‑CCCCACAGCTGATGAACCGTCCCCCGCCATATTCACGCTCAGAAAGAGaaaa                                                                           >  1:102988/1‑250 (MQ=255)
                                                                                                                                                             tgtTGATACTGGAATTTATCGGAATGATGCTACTGGCGGTGGCGCTGCTCTCGGGCAAAGACTCCCTGTCGCTGCC‑TC‑AGCCATTTTTTCGGCCAGAAATGCAGATTTTGATGGTTTTTTTCGGGG‑TTTTTCTCATGCTTCCCGCTGCGGGGGGGGTTTTTTTT‑TAGGGGGGAAAACGTCCTT‑CCCCCCCGCTGATGAACCGTCCACCGCAATATTTACCTTCAGAAAGAGAAAAAGATAATGACGcccac                                                           >  1:23009/1‑248 (MQ=255)
                                                                                                                                                                tGATACTGGAATTTATCGGAATGATGCTACTGGCGGAGGCGATGCTGGTGGTTAGAGACTCCCTGTCGCTGCC‑TG‑AGACCTTTTTTTTGCCAGGAGAGCAGATTCTGATGATTTTTCTCCGGG‑TTTTGCTCCTGCTTTCCCCTGCGGTGGGGGGTATTTTT‑CCGGGGGCAAAACGTCTTGACCCCA‑AGCTGCTGAAACGTCCCCCCCAAAATTAACGTTCAGAAAAAGGAAAACATAATGACGACAAccc                                                         >  1:21863/1‑249 (MQ=255)
                                                                                                                                                                   tACTGGAATTTATCGGAATGATGCTACTGGCGGTGGCGCTGTCGTCGGGAAGCGACCCCCCGCCGGTGCC‑TGGACCCTTTTTTCCGGCC‑GGAGGGGAGAGTCTTGTGATTTTTCTCCGCG‑TTTTGGTCCCGGTTCCCGCCGCGGGGGGGGTTATTCTTTCTGG‑GGCCAAACGTCTTG‑CCCCCCAGCCGTAGAAACGTCCACCCCACTATTTACGTTCCGAAAGAGAAAAAAATACAGATGCCAACCagtgt                                                     >  2:202186/1‑247 (MQ=255)
                                                                                                                                                                            ttATCGGAATGATGCTACTGGCGGTGGCGCTGCTGTCGGTAAGCGACTCCCTGTCGCTGCC‑TG‑AGCCATTTTCTCGGCCAGAAGTGCAGATTCTGATGATTTTTCTCGGTG‑TTTTGCTCATGCTTCCCGCTGCGGTGGTGGTTATTCTT‑CAGGTGGCAAAACGTCTTG‑CCCCACAGCTGATGAACCGTCCACCGCAATATTCACGTTCAGAAAGAGAAAAAGATAATGACGCCAACCATTGAACTTAttt                                             <  1:288928/250‑1 (MQ=255)
                                                                                                                                                                                            aCTGGCGGTGGCGCTGCTGTCGGTAAGCGACTCCCTGTCGCTGCC‑TG‑AGCCATTTTCTCGGCCAGAAGTGCAGATTCTGATGATTTTTCTCGGTG‑TTTTGCTCATGCTTCCCGCTGCGGTGGTGGTTATTCTT‑CAGGTGGCAAAACGTCTTG‑CCCCACAGCTGATGAACCGTCCACCGCAATATTCACGTTCAGAAAGAGAAAAAGATAATGACGCCAACCATTGAACTTATTTGTGGCCATCGCTCCa                              <  1:78928/249‑1 (MQ=255)
                                                                                                                                                                                              tGGCGGTGGCGCTGCTGTCGGTAAGCGACTCCCTGTCGCTGCC‑TG‑AGCCATTTTCTCGGCCAGAAGTGCAGATTCTGATGATTTTTCTCGGTG‑TTTTGCTCATGCTTCCCGCTGCGGTGGTGGTTATTCTT‑CAGGTGGCAAAACGTCTTG‑CCCCACAGCTGATGAACCGTCCACCGCAATATTCACGTTCAGAAAGAGAAAAAGATAATGACGCCAACCATTGAACTTATTTGTGGCCATCGCTCCATTCg                          >  1:151480/1‑251 (MQ=255)
                                                                                                                                                                                                                      gCGACTCCCTGTCGCTGCC‑TG‑AGCCATTTTCTCGGCCAGAAGTGCAGATTCTGATGATTTTTCTCGGTG‑TTTTGCTCATGCTTCCCGCTGCGGTGGTGGTTATTCTT‑CAGGTGGCAAAACGTCTTG‑CCCCACAGCTGATGAACCGTCCACCGCAATATTCACGTTCAGAAAGAGAAAAAGATAATGACGCCAACCATTGAACTTATTTGTGGCCATCGCTCCATTCGCCATTTCACTGATGAACCCATTTc  <  1:134162/251‑1 (MQ=255)
                                                                                                                                                                                                                             ccTGTCGCTGCC‑TG‑AGCCATTTTCTCGGCCAGAAGTGCAGATTCTGATGATTTTTCTCGGTG‑TTTTGCTCATGCTTCCCGCTGCGGTGGTGGTTATTCTT‑CAGGTGGCAAAACGTCTTG‑CCCCACAGCTGATGAACCGTCCACCGCAATATTCAc                                                                                           >  2:195749/1‑155 (MQ=255)
                                                                                                                                                                                                                             ccTGTCGCTGCC‑TG‑AGCCATTTTCTCGGCCAGAAGTGCAGATTCTGATGATTTTTCTCGGTG‑TTTTGCTCATGCTTCCCGCTGCGGTGGTGGTTATTCTT‑CAGGTGGCAAAACGTCTTG‑CCCCACAGCTGATGAACCGTCCACCGCAATATTCAc                                                                                           <  1:195749/155‑1 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                               
ATCACGCTAAAATGTATTCGCTGAAAGTAGGTTTAACCTGTTGCATTAATTGCTAAAAGCTATAACTGTTAAACACAATACAGTGAAAAGTTTTAGACTGAAGGCTCACTTTGCAGAGGGAAGCGTATGCGCGCGATCGGTAAATTGCCTAAAGGCGTGTTGATACTGGAATTTATCGGAATGATGCTACTGGCGGTGGCGCTGCTGTCGGTAAGCGACTCCCTGTCGCTGCC‑TG‑AGCCATTTTCTCGGCCAGAAGTGCAGATTCTGATGATTTTTCTCGGTG‑TTTTGCTCATGCTTCCCGCTGCGGTGGTGGTTATTCTT‑CAGGTGGCAAAACGTCTTG‑CCCCACAGCTGATGAACCGTCCACCGCAATATTCACGTTCAGAAAGAGAAAAAGATAATGACGCCAACCATTGAACTTATTTGTGGCCATCGCTCCATTCGCCATTTCACTGATGAACCCATTTC  >  NC_000913/890787‑891251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑