Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,606,2160AG32.1% 23.7 / 17.8 29noncoding (71/87 nt)leuPtRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base A (13/6);  new base G (4/5);  total (18/11)
Fisher's exact test for biased strand distribution p-value = 4.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.38e-02
Rejected as consensus: Frequency below/above cutoff threshold.

CTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATC‑TTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑G‑G‑GGGGGG‑A‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCT  >  NC_000913/4605975‑4606461
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      
cTACCATCGTGACTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAG‑CAACATTACTTTTTTTGATATACCCTCGTTTGGTGCGA‑GTG‑GGGTGG‑A‑CTTTAAc                                                                                                                                                                                                                                                >  2:236336/1‑251 (MQ=37)
                                catGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATC‑TTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑G‑G‑GGGGGG‑A‑CTTTAACCCCCCCGTCCGTAAGAACACTAACACCTGAAGc                                                                                                                                                                                                               >  1:135334/1‑251 (MQ=255)
                                   gCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATC‑TTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑G‑G‑GGGGGG‑GGCTTGAACCCCCAc                                                                                                                                                                                                                                          <  2:323400/222‑1 (MQ=255)
                                   gCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATC‑TTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑G‑G‑GGGGGG‑GGCTTGAACCCCCAc                                                                                                                                                                                                                                          >  1:323400/1‑222 (MQ=255)
                                     tgatgaTAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCCCACAGTCTTC‑TTACTTTTTTTGATATCCCCTCGTGTGGTCCGATGCG‑GGGGGT‑C‑CTTATACTCCCACGTCCGGAAGAACACTGACACCTGAGGCTat                                                                                                                                                                                                            >  2:304726/1‑250 (MQ=25)
                                               gAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATC‑TTACTTTTTTTGATATCACCTCGTTTGGTGCGA‑G‑G‑GGGGGG‑A‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGCAGCTAGCGCGTCTACCa                                                                                                                                                                                                 >  1:218873/1‑250 (MQ=255)
                                                           gggATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACACTCCTC‑TTACTTTTATTGATTTCGCCTCGTTTGGGGCGG‑G‑G‑GGGGGGGA‑CTTTAAACCCCTCGGCGGTAAGAACACCAACACCAAAAGCTaacccggctaccccatcccccccct                                                                                                                                                                                     >  2:317426/1‑227 (MQ=18)
                                                                                  cAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACCCCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTGCGCCCAGTCATC‑TTTCTTTTTTTGATATCGCCACGTTTGGTGCGA‑G‑G‑GGGGGGGA‑TTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGc                                                                                                                                                                          <  2:183229/239‑1 (MQ=25)
                                                                                  cAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATC‑TTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑G‑G‑GGGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGc                                                                                                                                                                          >  1:183229/1‑239 (MQ=255)
                                                                                   aaGAAAGGAAGTAAAGATAATTGGTGGGAGGGGGGGGGACTTGAACCCCCCCGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATC‑TTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑G‑G‑GGGGGG‑GGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAAc                                                                                                                                                             <  1:79794/251‑1 (MQ=255)
                                                                                      aaaGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATC‑TTACTTTTTTTGATATCTCCTCGTTTGGCGCGA‑G‑G‑GGGGGG‑A‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCCCCCAGTGCGAGTAATGTCATCGTgg                                                                                                                                                         >  1:104512/1‑251 (MQ=37)
                                                                                                      aTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATC‑TTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑G‑G‑GGGGGG‑A‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATAAACGTGGTTTTTGGTGCGCgggg                                                                                                                                         >  1:7391/1‑251 (MQ=255)
                                                                                                           cgagggggggggg‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTAATC‑TTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑G‑G‑GGGGGG‑A‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGGGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGAc                                                                                                                                   >  1:318275/6‑251 (MQ=255)
                                                                                                            cgaggggggggg‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATC‑TTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑G‑G‑GGGGGG‑A‑CTTGAACCCCCACGTCCGTAAGAACACTACCACCTGAAGCTAGCACGTCTACCAATCACGCCACCTACGCCCAGTGCGAGTAATATCAACGTGGTGTTTGGTTCGAGGGGAGGGACt                                                                                                                                  >  1:254060/5‑251 (MQ=37)
                                                                                                             gaggggggggg‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATC‑TTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑G‑G‑GGGGGG‑A‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATc                                                                                                                                                                >  2:195596/4‑220 (MQ=255)
                                                                                                             gaggggggggg‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATC‑TTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑G‑G‑GGGGGG‑A‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATc                                                                                                                                                                <  1:195596/217‑1 (MQ=255)
                                                                                                                                           gTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATC‑TTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑G‑G‑GGGGGG‑GGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCCCCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTa                                                                                                       >  1:173165/1‑249 (MQ=255)
                                                                                                                                           gTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATC‑TTACTTGTCTGGATATCGCCTCGTTTGGTGCGA‑G‑G‑GGGGGG‑GGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGGCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTa                                                                                                       <  2:173165/249‑1 (MQ=38)
                                                                                                                                                                       cgTCTACCAATTCCGCCACCTTCACACAGTCATC‑TTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑G‑G‑GGGGGG‑A‑CTTGAACCCCCACGTCCGTAAGAACACTAAAACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg                                                                        >  1:259511/1‑251 (MQ=255)
                                                                                                                                                                            aCCAATCCCGCCCCCTTCGCACAGACATC‑TTTCTTTTTTTGAAATCGCCTCGTTTGGTGCGA‑G‑G‑GGGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTCCCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACCCCTGAAGCTAGCGCGTCTACCAATTCCGCCAc                                                                    <  1:256579/251‑1 (MQ=37)
                                                                                                                                                                             ccAATTCCGCCACCTTCGCCCAGTCATC‑TTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑G‑G‑GGGGGG‑A‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCt                                                                  <  1:137854/251‑1 (MQ=255)
                                                                                                                                                                                    cGCCACCTTCGCACAGTCATC‑TTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑G‑GCGGGGGG‑A‑CTTGCACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGACCAATATCAACGTGGTTTTTGGAGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTACCACCTGAAGCTCGCGCGTCTACCAATTCCGCCACCTTCgaat                                                            >  1:188207/1‑248 (MQ=37)
                                                                                                                                                                                         ccTTCGCACAGTCATC‑TTACTTTTTTTGATAACGCCTCGGTTGGTGCGA‑G‑G‑GGGGGG‑GGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCAt                                                       <  1:25828/251‑1 (MQ=255)
                                                                                                                                                                                                            aCTTTTTTTGATATCGCCTCGTTTGGTGCGA‑G‑G‑GGGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAAtt                                     <  1:317426/251‑1 (MQ=255)
                                                                                                                                                                                                                           gCCTCGTTTGGTGCGA‑G‑G‑GGGGGG‑GGCTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGCGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACCCCTGAAGCTAGCGCGTCTACCAATTACGCAACCTTCGCATACCATCAATTATTAAAAAGAATTGCTAcaac                             >  2:273859/1‑241 (MQ=25)
                                                                                                                                                                                                                           gCCTCGTTTGGTGCGA‑G‑G‑GGGGGG‑GGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCAc                             <  1:273859/244‑1 (MQ=255)
                                                                                                                                                                                                                                          agg‑g‑gggggg‑G‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTGGtttt       >  1:127860/3‑251 (MQ=255)
                                                                                                                                                                                                                                                  gggg‑A‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCt  <  1:139806/250‑1 (MQ=255)
                                                                                                                                                                                                                                                  gggg‑A‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGCTGCGAGGGGGGGGACTTGGACCCCCACGTCCGTAGGGACACTAACACCTGAAGATAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCt  >  2:139806/1‑250 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      
CTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATC‑TTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑G‑G‑GGGGGG‑A‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCT  >  NC_000913/4605975‑4606461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑