Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,027,2330TA41.4% 21.9 / 12.7 29intergenic (+5/+376)fre/fadANAD(P)H‑flavin reductase/3‑ketoacyl‑CoA thiolase
Reads supporting (aligned to +/- strand):  ref base T (11/6);  new base A (0/12);  total (11/18)
Fisher's exact test for biased strand distribution p-value = 3.90e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCG‑ATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGG  >  NC_000913/4026988‑4027478
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     
gcTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCG‑ATGCGTTTGCATTTATCTGAGATATaaaaa                                                                                                                                                                                                                                                  <  2:224038/251‑1 (MQ=255)
             cATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCAAGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGGCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCG‑ATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTg                                                                                                                                                                                                                                      >  1:27810/1‑250 (MQ=255)
                     tCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTTCAGGGTCACGGTAAGCTGGCAGAGGATGATATCTATATTTCCGGACGGTTTGTGATGGCGGAAATTGCCCCCGATCTGTTTTGCAGTGAGCGGAATGCGCGGGGAGATCGCCCGTTTGTCGCGTG‑GTTTGCCTTTTTCTGAGATATTACAAAACCCCCCCCCGCCAGGCggg                                                                                                                                                                                                                             >  2:228922/1‑251 (MQ=255)
                               gtgCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCCGGATCACGGGACGCCGGGAGAGCCTGGTATCTATATTTCCGGGCGTTTTGAGGTGGGGGAAATTGCCCGCGCCCTGTTTTGCAGTGAGCGTGATGGGCGGGGAGATCGCCTGTTTGGCG‑ATGCGGTTGCATTTATTTGGGATATAAAAAAACCCCCCCCTCACAGGCgggcgaacacagc                                                                                                                                                                                                                   >  2:233194/1‑241 (MQ=255)
                                     gtggtTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGGACTGCAGGCGCACGGTACGCTGGCCGAGCATGGAATATATTTTTCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGGTCTTTTTTGCAGTGAGCGGAATGCGCGGGGAGATCGCCCGTTTGGGG‑ATGCGTTTGCTTTTTTCTGAGATATAAAAAAACCCGCCCCTGACAGGGGGGAAGAAAGGCAACTaa                                                                                                                                                                                                              >  2:44561/1‑250 (MQ=255)
                                             acaaccggtggcgggtgggggTGGGGGTACTGGCACGGTGTAAACGGCGTTATTGCAGGATCGCGGAACGCTGGAAGGGAAGGTTATCTATATTGCCGGACGTTTGAAGATGGCAAAAAGTGCCCGCGATTTGTTTTGCAGTGAGCGTAAGGCGCGGAAAGATCCCCGTTTTGGCG‑AGGGGTTGGAATTTTGCTGAGTAAAAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTAtt                                                                                                                                                                                                     <  2:223925/231‑1 (MQ=255)
                                                           gcgggggggggCGTACTGGCACCGTGTTAGCGGCGGTATTGCAGGATCACGGTACGCGGGCAGAGCATGATATCTATATTGCGGGAGGTTTTGAGATGGAAAAAATTGCCCGCGATCTGTTTTGCAGGGAGCGTAGTGCGGGGGAAGACCGCTTGTTTGGCG‑ATGCTTTTGCTTTTATCTGAAATAAAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGa                                                                                                                                                                                       <  1:10835/243‑1 (MQ=255)
                                                             tggcgggggcgtacgcgccccggtttAACGGCTTTATTGCTGGATCACGGTACGCTGGAATATTGTCTTTTCTATTTTTCCGGACTTTTTGATAGGGCTAAAATGCCCCGCGATTTTTTTTGCTTTGAGCTTAATGCGCGGAAAGATCTCTTTTTTGGCG‑ATGCGTTTGCATTAATCTGAAATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCTACTAAACTGTTATTCAGTGGCATTTAGAt                                                                                                                                                                                      <  1:275069/226‑1 (MQ=255)
                                                                ggTGGGCGTACGGGCCCGGTGTTAACGGCTGTATAGAAGGATCACGGTACGCGGGGAGAGCATGATAGTTATATGGCCGGCCGTTTTGAGAGGGCGAAAATTGCCCGGGGTCTGTTTTGCGGTGAGCGTAATGCGGGGGAAGACCGCCTGTTTGGCG‑AGGCGTTTGAATTTATCTGAGATAAAAAAAACCCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTAt                                                                                                                                                                                  <  2:225675/250‑1 (MQ=255)
                                                                   ggggttatgggCACCGTTTTAACGGCGGTATTGAAGGATAAGGGGCGGCGGGCAGACCAGAATATTTATTTTGCGGTATGTTTTGAGAGGGCAAAAATGGCCCGCTTTCTTTTTTGAGGTGAGCGTAATGCGGGGGAAGATCGCTTTTTGGGCG‑AGGCTTTTGCATTTATCGGAGAAATAAAAAACCCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGAc                                                                                                                                                                               <  2:335092/242‑1 (MQ=255)
                                                                         ccgggaacggtgtTAAGGGCGTTTTTGCAGGATAACGGTACGTGGGAAGCGAAGTATAGCTATATTGCGGGCCGTTTTGAGTGGGCAAAATTTGCCCGCGATCTGTTTGGCGGTGAGCGTAAGGCGCGGAAAAATCGCTTGTTTGGGG‑ATGGGTTTGAATTTATCTAAGATAAAAAAAACCCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATc                                                                                                                                                                          <  2:267190/241‑1 (MQ=255)
                                                                                  gtgtTAACGGGGGTATTGAAGGATCAGGGTAGGCTGGAAGAGCAGGATATTTATTTTGCGGGAGGTTGGGAGATGGCAAAAATGGCCCGCGATCTGTTTTGAGGTGAGGGTAATGCGGGGGAAGATCGCTTTTTGGGCG‑GGGCGTTTGAATTTATCTGAGATAAAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCa                                                                                                                                                                <  1:52573/251‑1 (MQ=255)
                                                                                                                   cTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCG‑ATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGc                                                                                                                               >  1:191253/1‑251 (MQ=255)
                                                                                                                                  ttCTATTTTGCGGGACGTTTTGAAGTGGAGAAAATTGCCGGCGATCTGTTTTGCAGTGAGGTTAAGGCGCGGGAAGACCGCCTTTTTGGGA‑ATGCGTTTGAATTTATCTGAGATAAAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCg                                                                                                                 <  2:199139/249‑1 (MQ=255)
                                                                                                                                              ggACTTTTTGAGAGGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGGGCGTAATGCGCGGGAAGATCGCCTGTTTGGCG‑ATGCGTTTGCATTTATCTGAGATAAAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCgg                                                                                                    <  1:237523/251‑1 (MQ=255)
                                                                                                                                                  gTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCG‑ATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTTGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGACGGATa                                                                                                 >  1:357888/1‑250 (MQ=255)
                                                                                                                                                        agaTGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCG‑ATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGAAGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGtt                                                                                          >  1:236898/1‑251 (MQ=255)
                                                                                                                                                                    gtgccggcattcttgtttGCAGTGTGCGAAATGCGAGGGAGGCTCGCCTTTTTGCCG‑ATGCGTTTGCATTTTTCGGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGTCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATc                                                                               <  1:17619/235‑1 (MQ=37)
                                                                                                                                                                     tccccggatctgtttgggaagggggCGTATTGCGGGGAAAGTTCGCTTGTTTGGCG‑TTGCTTTTGCATTTATCTGATATAAAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATcc                                                                              <  2:347843/226‑1 (MQ=25)
                                                                                                                                                                                         gtgggcttAATGCGCGGGAAGATCGCCTTTTTGGCG‑ATGCGTTTGCATTTATCTGAGATAAAAAAAAACCCGCCCCTGACAGGTGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCt                                                         <  1:44561/244‑1 (MQ=255)
                                                                                                                                                                                         gTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCG‑ATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCCTGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGGGCATGATGCCt                                                         >  2:245444/1‑251 (MQ=255)
                                                                                                                                                                                             gtgaagggcgggggAAAATCGCCTGTTTGGCG‑ATGGTTTTGTATTTATCTGAGATAAAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGAGGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATg                                                     <  2:333511/240‑1 (MQ=25)
                                                                                                                                                                                                                cGCCTGTTTGGCG‑ATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTAt                                  >  1:142641/1‑251 (MQ=255)
                                                                                                                                                                                                                 gCCTGTTGGGCT‑AGGCGTTTGATTTTATCTGATATAAAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATTCCTGATGCGACGCTGGCGCGTCTTATc                                 <  1:93880/251‑1 (MQ=21)
                                                                                                                                                                                                                          gCG‑ATGCGTTTGAATTTATCGGAGATAAAAAAAACCCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTACa                        <  1:309910/251‑1 (MQ=255)
                                                                                                                                                                                                                                gcgttgtcATTTATCTGAGATATAAAAAACCCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCGATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAg                   <  1:18416/244‑1 (MQ=255)
                                                                                                                                                                                                                                  gTTTGCATTTATCGGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTc                 <  1:118780/251‑1 (MQ=255)
                                                                                                                                                                                                                                                gagaTATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGAGGCCTTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCg   >  1:21311/1‑251 (MQ=255)
                                                                                                                                                                                                                                                 agaTATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCCACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCGTGCCTACAAATATTCCGAACCGTAGGCCgg  >  1:350545/1‑251 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     
GCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCG‑ATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGG  >  NC_000913/4026988‑4027478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑