Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,140,9110TC30.8% 29.0 / 15.6 26K234K (AAA→AAG) asmAputative assembly protein AsmA
Reads supporting (aligned to +/- strand):  ref base T (10/8);  new base C (4/4);  total (14/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.25e-01
Rejected as consensus: Frequency below/above cutoff threshold.

CTGCCCCTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAGGTTGTCAAGATTCAGTTGCGGGAATTGCAGCCTCAGCTGCCATTCCGGTTTCTCTGTCAGCGTAACCTGTGCTTGCCCGCTTAGCGTACTATCATTGGCGGTCAAACTAATTTGGTTAAATGAAAGGCG‑TTTATGTGACTCCTGCCACTGGGCCTGGAAACTCCCCTGCCCCTGAATACCTTGTTTTGGTAAATCGGCACCCTGCAACTGCCAGTTAATTTGTTCAATAGCCGCCGTTAAATCATGCGGATAATCAGAAGCATCTACCGTACCGTTAAGGGATATCGTGAGATCGCGCTGATCGCGATTAACCCGCCCGGAGAACTCAAATGAACCACGATGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCAGCAC  >  NC_000913/2140671‑2141147
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                            
cttccccGGCTGCGCGTCACCGTGTTCACCATTCGCGTTTTCATTAAGCGGGATGAGGTTGTCAAGATTCAGTTGCGGGAATTGCAGCCTCAGCTGCCTTTCCGGTTTCTCTGTCAGCGTAACCTGTGCTTGCCCGCTTAGCGTACTATCATTGGCGTTCAACCTAATTTGGTTAAATTAAAGCCG‑TTTATGTGACTCCTGCCACTGGGCCTGGAAACTCCCCTGCCCCGGAATACCTTGTTTTGGTaa                                                                                                                                                                                                                                      <  2:160830/246‑1 (MQ=255)
  gCCCCTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAGGTTGTCAAGATTCAGTTGCGGGAATTGCAGCCTCAGCTGCCATTCCGGTTTCTCTGTCAGCGTAACCTGTGCTTGCCCGCTTAGCGTACTATCATTGGCGGTCAAACTAATTTGGTTAAATGAAAGGCG‑TTTATGTGACTCCTGCCACTGGGCCTGGAAACTCCCCTTCCCCTGAATACCTTGCTTTGGTAAATc                                                                                                                                                                                                                                   <  1:324971/250‑1 (MQ=255)
                     gTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAGGTTGTCAAGATTCAGTTGCGGGAATTGCAGCCTCAGCTGCCATTCCGGTTTCTCTGTCAGCGTAACCTGTGCTTGCCCGCTTAGCGTACTATCATTGGCGGTCAAACTAATTTGGTTAAATGAAAGGCG‑TTTATGTGACTCCTGCCACTGGGCCTGGAAACTCCCCTGCCCCTGAATACCTTGCTTTGGTAAATCGGCACCCTGCAACTGCCAGt                                                                                                                                                                                                               <  1:251307/251‑1 (MQ=255)
                               ttCGCGGTTTCAGTAAGCGGGATGAGGTTGTCAAGATTCAGTTGCGGGAATTGCAGCCTCAGCTGCCTTTCCGTTTTCTCTGTCAGCGTAACCTGTGCTTGCCCGCTTAGCGTACTATCATTGGCGGTCAAACTAATTTGGTTAAATGAAAGGCG‑TTTATGTGAGTCCTGCCACTGGGCCTGGAAACTCCCCTGCCCCTGAATACCTTGTTTTGGTAAATCGGCACCCTGCAACTGCCAGTTAATTTGt                                                                                                                                                                                                       <  2:371887/249‑1 (MQ=255)
                                    ggTTTCATTAAGCGGGATGAGGTTGTCAAGATTCAGTTGCGGGAATTGCAGCCTCAGCTGCCATTCCGGTTTCTCTGTCAGCGTAACCTGTGCTTGCCCGCTTAGCGTACTATCATTGGCGGTCAAACTAATTTGGTTAAATGAAAGGCG‑TTTATGTGACTCCTGCCACTGGGCCTGGAAACTCCCCTGCCCCTGAATACCTTGTTTTGGTAAATCGGCACCCTGCCACTGCCAGGTAATTTGTTCAATat                                                                                                                                                                                                >  2:195194/1‑250 (MQ=255)
                                           ttAAGCCGGTTGAGGTTCGCAAGATTAAGATGCGGGAATTGCAGACTCAGCTGCCATTCCGGTTTCTCTGTCAGCGTAACCTGTGCTTGCCCGCTTACCGTACTATCATTGGCGGCCAAACTAATTTGGTTCATTGAACGGCG‑ATTAAGTGACCCCTTCCACTGGGCCCGGTAACTCCCCTACCCCTGAAAAACTTGCTTTGGTAAATCGGGAACCCACACAAGCCAGTTAATTTGGTCAATAgcccacgg                                                                                                                                                                                         >  2:333024/1‑246 (MQ=255)
                                                 gggATGAGGTTGTCAAGATTCAGTTGCGGGAATTGCAGCCTCAGCTGCCATTCCGGTTTCTCTGTCAGCGTAACCTGTGCTTGCCCGCTTAGCGTACTATCATTGGCGGTCAAACTAATTTTGTTAAATGAAAGGCG‑TTTATGTGACTCCTGCCACTGGGCCTGGAAACTCCCCTGCCCCTGAATACCTTGTTTTGGTAAATCGGCACCCTGCCACTGCCCGTTAATTTTTTCAATAGCCGCCGTTAAATc                                                                                                                                                                                   >  2:131230/1‑251 (MQ=255)
                                                                      aGTTGCGGGAATTGCAGCCTCAGCTGCCATTCCGGTTTCTCTGTCAGCGTAACCTGTGCTTGCCCGCTTAGCGTACTATCATTGGCGGTCAAACTAATTTTGTTAAATGAAAGGCG‑TTTATGTGACTCCTGCCACTGGGCCTGGAAAATCCCCTGCCCCTGAATACCTTGTTTTGGTAAATCGGCACCCTGCAACTGCCAGTTAATTTGTTCAATAGCCGCCGTTAAATCATGCGGCTAATCAGAAGCATc                                                                                                                                                              >  1:142534/1‑251 (MQ=255)
                                                                       gTTGCGGGAATTGCAGCCTCAGCGGCCATTCCGGTTTCTCTGTCAGCGTACCCTGTGCTTGCCCGCTTAGCGTACTATCATTGGCGGTCAAACTAATTTGGTTAAATGAAAGGCG‑TTTATGTGCCTCCTGCCACTGGGCCTGGAAACCCCCCTGCCCCTGAATACCTTGTTTTGGTAAATCGGCACCCTGCAACTGCCAGTTAATTTGTTCAATAGCCGCCGTTAAATCATGCGGATAATCAGAAGCATCt                                                                                                                                                             <  1:102078/251‑1 (MQ=255)
                                                                                aTTGCAGCCTCAGCTGCCATTCCGGTTTCTCTGTCAGCGTAACCTGTGCTTGCCCGCTTAGCGTACTATCATTGGCGGTCAAACTAATTTGGTTAAATGAAAGGCG‑TTTATGTGACTCCTGCCACTGGGCCTGGAAACTCCCCTGCCCCTGAATACCTTGTTTTGGTAAATCGGCACCCTGCAACTGCCAGTTAATTTGTTCAATAGCCGCCGTTAAATCATGCGGATAATCAGAAGCATCtaccgtaccg                                                                                                                                                    >  1:96831/1‑251 (MQ=255)
                                                                                                         tttCTCTGTCAGCTTAACATGTGCTTTCCCGCTTAGCGTACTATCATTGGCGGTCTAACTAATTTGGTTAAATGAAAGGAG‑TTTATGTGACCCCTGCCACTGGGCCTGGAAACTCCCCTGCCCCTGAATAACTTTTTTTGTTAAATCCGCACCCTGCAACCTCCAGTTAATTTGTTCCATAACCTCCGTTAAATCCTGCGGATAATCAAAAGCATCTACCTTACACTTAAGGGATATCGTGAGATCTCGCt                                                                                                                           >  2:41150/1‑251 (MQ=255)
                                                                                                                        aaCCTGTGCTGGCCCGGTTAGCGTACTACCATGGGCGGACAAACTAATTTGGT‑AAAAGAAAGGGGGTTTATGTGACCCCTGCCACTGGGCCTGGAAACCCCCCTGCCCCTGAATACCTTGTTTTGGTAAATCGGCACCCTGCAACTGCCAGTTAATTTGTTCAATAGCCGCCGTTAAATCATGCGGATAATCAGAAGCATCTACCGTACCGTTAAGGGATATCGTGAGATCGCGCTGATCGCGATTAAccc                                                                                                            <  2:163257/251‑1 (MQ=255)
                                                                                                                               gtttGCCCGCTTAGCTAACTATCATTGGCGGACAAACTATTTTGTGAAAATGAAGGGCT‑TTTAGTTGCCTCTGGCCACTGGGCCTGGAACCTCCCCTGCCCCTGAATACCTTGTTTTGGTAAATCGGCCCCCTGCAACTGCCAGTTAATTTGTTCAATAGCCGCCGTTAAATCATGCGGATAATCAGAAGCATCTACCGTACCGTTAAGGGATATCGTGAGATCGCGCTGATCGCGATTAACCCGCCCgg                                                                                                      <  1:251910/248‑1 (MQ=255)
                                                                                                                                                          gCGGTCAAACTAATTTGGTTAAATGAAAGGCG‑TTTATGTGACTCCTGCCACTGGGCCTGGAAACTCCCCTGCCCCTGAATACCTTGTTTTGGTAAATCGGCACCCTGCAACTGCCAGTTAATTTGTTCAATAGCCGCCGTTAAATCATGCGGATAATCAGAAGCATCTACCGTACCGTTAAGGGATATCGTGAGATCGCGCTGATCGCGATTAACCCGCCCGGAGAACTCACATGAACCACGATGTTgggg                                                                          >  2:97419/1‑251 (MQ=255)
                                                                                                                                                          gCGGTCAAACTAATTTGGTTAAATGAAAGGCG‑TTTATGTGACTCCTGCCACTGGGCCTGGAAACTCCCCTGCCCCTGAATACCTTGCTTTGGTAAATCGGCACCCTGCAACTGCCAGTTAATTTGTTCAATAGCCGCCGTTAAATCATGCGGATAATCAGAAGCATCTACCGTACCGTTAAGGGATATCGTGAGATCGCGCTGATCGCGATTAACCCGCCCGGAGAACTCAAATGAACCACGATGTTgggg                                                                          >  1:215222/1‑251 (MQ=255)
                                                                                                                                                            ggTCAAACTAATTTGGTTAAATGAAAGGCG‑TTTCTGTGACTCCTGCCACTGGGCCTGGAAACTCCCCTGCCCCTGAATACCTTGTTTTGGTAAATCGGCACCCTGCAACTGCCAGTTAATTTGTTCAATAGCCGCCGTTAAATCATGCGGATAATCAGAAGCATCTACCGTACCGTTAAGGGATATCGTGAGATCGCGCTGATCGCGATTAACCCGCCCGGAGAACTCAAATGAACCACGATGTTGGGGAt                                                                        >  2:369866/1‑251 (MQ=255)
                                                                                                                                                              taaaaCTAATTGGTTTAAAGGAAGGGCG‑TTTATGTGCCCCCTGCCACTGGGCCGGGAAACTCCCCTGCCCCTGAATACCTTGCTTTGGTAAATCGGCACCCTGCAACTGCCAGTTAATTTTTTCAATAGCCGCCGTTAAATCATGCGGATAATCAGAAGCATCTACCGTACCGTTAAGGGATATCGTGAGATCGCGCTGATCGCGATTAACCCGCCCGGAGAACTCAAATGAACCACGAt                                                                                 <  1:276814/238‑1 (MQ=255)
                                                                                                                                                              tCAAACTAATTTGGTTAAATGAAAGGCG‑TTTATGTGACTCCTGCCACTGGGCCTGGAAACTCCCCTGCCCCTGAATACCTTGCTTTGGTAAATCGGCACCCTGCAACTGCCAGTTAATTTGTTCAATAGCCGCCGTTAAATCATGCGGATAATCAGAAGCATCTACCGTACCGTTAAGGGATATCGTGAGATCGCGCTGATCGCGATTAACCCGCCCGGAGAACTCAAATGAACCACGAt                                                                                 >  2:276814/1‑240 (MQ=255)
                                                                                                                                                                     aaTTTGGTTAAATGAAAGGCG‑TTTATGTGACTCCTGCCACTGGGCCTGGAAACTCCCCTGCCCCTGAATACCTTGCTTTGGTAAATCGGCACCCTGCAACTGCCAGTTAATTTGTTCAATAGCCGCCGTTAAATCATGCGGATAATCAGAAGCATCTACCGTACCGTTAAGGGATATCGTGAGATCGCGCTGATCGCGATTAACCCGCCCGGAGAACTCAAATGAACCACGATGTTGGGGATCTTGTTcc                                                                >  2:305201/1‑250 (MQ=255)
                                                                                                                                                                      aTTTGGTTAAATGAAAGGCG‑TTTATGTGACTCCTGCCACTGGGCCTGGAAACTCCCCTGCCCCTGAATACCTTGTTTTGGTAAATCGGCACCCTGCAACTGCCAGTTAATTTGTTCAATAGCCGCCGTTAAATCATGCGGATAATCAGAAGCATCTACCGTACCGTTAAGGGATATCGTGAGATCGCGCTGATCGCGATTAACCCGCCCGGAGAACTCAAATGAACCACGATGTTGGGGATCTTGTTCCAt                                                              >  1:248727/1‑251 (MQ=255)
                                                                                                                                                                                   aaaggggttttatggccccccTGCCATTGGGCCTGGAACCTCCCCTGCCCATGAATCCCTTGTTTTGGTAAACCGGCACCCTGCAACTGCCAGTTAATTTGTTCAATAGCCGCCGTTAAATCATGCAGATAATCAGAAGCATCTACCGTACCGTTAAGGGATATCGTGAGATCGCGCTGATCGCGATTAACCCGCCCGGAGAACTCAAATGAACCACGATGTTGGGGATCTTGTTCCATTTGCAGGCGAat                                                  <  2:254401/232‑1 (MQ=255)
                                                                                                                                                                                                   ccccttGCCGCTGGGCCTGGAACCCCCCCGGCCCTTAAATCCTTTGTTTGGGAAAATCGCCCCCCGGAAAGTCCCAGTAAATTTGTTCAATGGCCGCCGTTAAATCATGCGGATAATCAGAAGCATCTACCGTACCGTTAAGGGATATCGTGAGATCGCGCTGAGCGCGATTAACCCGCCCGGAGAACTCAAATGAACCACGATGTTGGGGATCTTGTTCCAGTTGCATGCGAATATTGCTGGTTGTCAcc                                  <  1:300935/246‑1 (MQ=255)
                                                                                                                                                                                                      ccTGCCACTGGGCCTGGAAACTCCCCTGCCCCTGAATACCTTGTTTTGGTAAATCGGCACCCTGCAACTGCCAGTTAATTTGTTCAATAGCCGCCGTTAAATCATGCGGATAATCAGAAGCATCTACCGTACCGTTAAGGGATATCGTGAGATCGCGCTGATCGCGATTAACCCGCCCGGAGAACTCAAATGAACCACGATGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTTCGGATTGTCACCTGc                               >  2:91752/1‑251 (MQ=255)
                                                                                                                                                                                                                 gCCTGAAAACTCCCCTGCCCCTGAATCCTTTGCTTGGGTAAATCGGCCCCCTGCCACTGCCAGTTAATTTGTTCAATAGCCGCCGTTAAATCATGCGGATAATCAGAAGCATCTACCGTACCGTTAAGGGATATCGTGAGATCGCGCTGATCGCGATTAACCCGCCCGGAGAACTCAAATGAACCACGATGTTGGGGATCTTGTTCCATTTGCGGGCTAATATTGCGGATTGTCACCTGCTCGTCATCTTc                    <  1:333024/251‑1 (MQ=255)
                                                                                                                                                                                                                          cTCCCCTGCCCCTGAATACCTTGTTTTGGTAAATCGGCACCCTGCAACTGCCAGTTAATTTGTTCAATAGCCGCCGTTAAATCATGCGGATAATCAGAAGCATCTACCGTACCGTTAAGGGATATCGTGAGATCGCGCTGATCGCGATTAACCCGCCCGGAGAACTCAAATGAACCACGATGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGa            >  2:38748/1‑250 (MQ=255)
                                                                                                                                                                                                                             ccctgcccctgAATCCTTTTTTTTGGTAAATCGGCCCCCTGAAACTGCCAGTTATTTTGTTCAATAGCCGCCGTTAAATCATGCGGATAATCAGAAGCATCTACCGTACCGTTAAGGGATATCGTGAGATCGCGCTGATCGCGATTAACCCGCCCGGAGAACTCAAATGAACCACGATGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAacac        <  1:290932/251‑1 (MQ=255)
                                                                                                                                                                                                                                   ccctaaatccttttttttGGTAAATCGCCCCCCTGCAACTGCCAGTTAATTTGTTCAATAGCCGCCGTTAAATCATGCGGATAATCAGAAGCATCTACCGTACCGTTAAGGGATATCGTGAGATCGCGCTGATCGCGATTAACCCGCCCGGAGAACTCAAATGAACCACGATGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCAGCAc  <  2:96831/237‑1 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                            
CTGCCCCTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAGGTTGTCAAGATTCAGTTGCGGGAATTGCAGCCTCAGCTGCCATTCCGGTTTCTCTGTCAGCGTAACCTGTGCTTGCCCGCTTAGCGTACTATCATTGGCGGTCAAACTAATTTGGTTAAATGAAAGGCG‑TTTATGTGACTCCTGCCACTGGGCCTGGAAACTCCCCTGCCCCTGAATACCTTGTTTTGGTAAATCGGCACCCTGCAACTGCCAGTTAATTTGTTCAATAGCCGCCGTTAAATCATGCGGATAATCAGAAGCATCTACCGTACCGTTAAGGGATATCGTGAGATCGCGCTGATCGCGATTAACCCGCCCGGAGAACTCAAATGAACCACGATGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCAGCAC  >  NC_000913/2140671‑2141147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑