Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	458,790	IS186 (+) +6 bp :: Δ1 bp	intergenic (+90/‑93)	clpX → / → lon	ATP‑dependent Clp protease ATP‑binding subunit ClpX/Lon protease

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	15387 =	NA (NA)	31 (0.870)	31/478	0.3	100%	noncoding (1/1345 nt)	IS186	repeat region
	?	NC_000913	= 458795	0 (0.000)					intergenic (+95/‑93)	clpX/lon	ATP‑dependent Clp protease ATP‑binding subunit ClpX/Lon protease
*	?	NC_000913	458790 =	0 (0.000)	29 (0.810)	26/480	0.4	100%	intergenic (+90/‑98)	clpX/lon	ATP‑dependent Clp protease ATP‑binding subunit ClpX/Lon protease
	?	NC_000913	= 609350	NA (NA)					noncoding (1344/1345 nt)	IS186	repeat region

GTCGTCGAAACACCGGTAGGCCTGAACGAAAGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCT‑GGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGC‑GAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTACGGCCCCGGGGGGATGTCATTACGTGAAGTCACTGCATGGGC  >  NC_000913/15143‑15635                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                            gtcgtcGAAACACCGGTAGGCCTGAACGAAAGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCAt                                                                                                                                                                                                                                                        <  1:57565/250‑1 (MQ=255)                gTAGGCCTGAACGAAAGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAgg                                                                                                                                                                                                                                        <  1:238828/251‑1 (MQ=255)                           cGAAAGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCGCCTGTAAAGTGGCGTGTATGACTCCCATAAGCGCTAACTTAAGGG‑TTGTGGTAtt                                                                                                                                                                                                                            <  2:336367/251‑1 (MQ=255)                                ggCAGAAACAGCGGATGCAAGGGCGGCAAGAAAGCTGCGGAGGCCCAACCGGCGAGCGCACCCGCCCGCGCTCAAAGAGCTTCTATGATGGTGTGAAGAAGTGGTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCGTGGGTAAAAATAGGGTGAGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGG‑Ttgttttagtacgcc                                                                                                                                                                                                                       <  2:284119/251‑12 (MQ=255)                                                 caagagctgcaagaAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGTCACCCCCCCAAACGCCAACTTATGGG‑GTTTGGGATTTCGCCCGATATGCttttacacg                                                                                                                                                                                                      >  1:212744/1‑245 (MQ=255)                                                   agagctgcaagaAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTGcc                                                                                                                                                                                                    <  1:329321/251‑1 (MQ=255)                                                      gctgcaagaAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTGCCGAt                                                                                                                                                                                                 <  1:103431/251‑1 (MQ=255)                                                          caagaAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCCTCAGAGGCAAATTAAAGG‑TTGTGGGGTTACGCCTGGTATTAtttttaatgccgctgaa                                                                                                                                                                                              >  1:356575/1‑236 (MQ=255)                                                           aagaAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGTCACTCCCCTAAACGCTAACTTAAGGGGTTG‑GGGATTACTCCTGATATGtttttaagggccggtggatt                                                                                                                                                                                            >  1:318394/1‑235 (MQ=255)                                                                aGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCACGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGGCACTCCCCCAAACGCCAACTTAAAGG‑GTGTGGGTTTAAGCCTGCTATTATTTTACATGCCGGTGAATTActct                                                                                                                                                                                       >  1:35847/1‑251 (MQ=255)                                                                                         acaacaGCCCGCGCTAAAAGAGCTGCTTTGATGGTGTGAAGAAGGTTTTTGACGACCGGACCCGCTAACCTCCCTAAAAGCCTGCCCGTGGGCAGGCCTGTGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTGAAGGT‑TTGTGGTATTGCGCGTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAGCTGGGCAACAATTCT‑gg                                                                                                                                                             <  2:176419/251‑1 (MQ=37)                                                                                                      cTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGTCACTCCCCTAAACGCTAACTTTAGGG‑GTGTGGTTTTTTGCCCTTTATGTTTTTACCTGCCGATGAATTAATTTCCCGATAACTGGTCCACAATTCTTGGccctctttgta                                                                                                                                                  >  1:203891/1‑242 (MQ=255)                                                                                                        cAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCT‑GGCCCATATTGGTAAg                                                                                                                                               <  2:318394/250‑1 (MQ=255)                                                                                                              gCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCT‑GGCCCATATTGGTAAGCCCgaag                                                                                                                                        <  1:100872/251‑1 (MQ=255)                                                                                                                 tcttTGATGGTGTGAAGAAGTTTTTTGACGACCGGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCT‑GGCCCATATTTGTAAGCCCgaaga                                                                                                                                       <  2:56012/249‑1 (MQ=255)                                                                                                                   tttGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCT‑GGCCCATATTGGTAAGCCCGAAGAACt                                                                                                                                    <  2:118738/250‑1 (MQ=255)                                                                                                                      gATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACGCTCACGATAACTGGTCAGCAATTCT‑GGCCCATATTGGTAAGCCCGAAGAACTGGa                                                                                                                                 <  2:331874/250‑1 (MQ=255)                                                                                                                            gtgAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCT‑GGCCCATATTGGTAAGCCCGAAGAACTGGATACTTc                                                                                                                           <  2:117310/250‑1 (MQ=255)                                                                                                                            gtgAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCT‑GGCCCATATTGGTAAGCCCGAAGAACTGGATACTTc                                                                                                                           <  2:287779/250‑1 (MQ=255)                                                                                                                                  aagTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGGGACTCCCAAAAACGCTAAATTTAGGG‑GTGGGGGATTACGCCTGATATGATTTAAAGTGCCGATGAATTAATCTCACGCTAAATGGGGAGCAATACT‑TGCCCATATTTGTAAGACCGAAGAACTgggaaattcggcaggc                                                                                                                    >  1:104841/1‑237 (MQ=255)                                                                                                                                                                  ccAAAAGACTGGCCGTGGGCAGGCGTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGGGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCG‑GGCGCATATTGTTAAGCCCGAAGAACGGGATACTTCGGCACGTAATGGCGGGGCTCTACCCCGCCGGCGC‑GAAAt                                                                                   <  2:363700/251‑1 (MQ=18)                                                                                                                                                                         ccTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCT‑GGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGC‑GAAATTCGTGAt                                                                            <  1:217390/251‑1 (MQ=255)                                                                                                                                                                                  gggCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAAGTACTCTCACGATAACTGGTCAGCAATTCT‑GGCCCATATTGGTAAGCCCGAAGAACTGGATGCTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGC‑GAAATTCGTGATGCTGCAAc                                                                    <  2:34806/250‑1 (MQ=255)                                                                                                                                                                                     cAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCT‑GGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGC‑GAAATTCGTGATGCTGCAACTCTg                                                                <  2:115272/251‑1 (MQ=255)                                                                                                                                                                                         ccTGGGAAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCCAAATTTAGGG‑GTGTGGGATTTCGCCTGATATGATTTTACGTGCCGCTGAATTAATCTCACCATAACTGGGCAGCAAATCT‑GGGCCATATTTGTAAACCCCAAAAACTGGATACTTCCGCACCGAATGCCGGGGCTCTAAAACCCCCCCGCCGAAAT‑CCTGATGGTGCAACTCTGCTAc                                                            >  1:287008/1‑251 (MQ=255)                                                                                                                                                                                                                           aGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCT‑GGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGC‑GAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTACGGCCCCGGGGGGATGTc                          <  1:310186/251‑1 (MQ=40)                                                                                                                                                                                                                             cACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGACAGCAATTCT‑GGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGC‑GAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTACGGCCCCGGGGGGATGTCAt                        <  1:202696/251‑1 (MQ=255)                                                                                                                                                                                                                                      aGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCT‑GGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGC‑GAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTACGGCCCCGGGGGGATGTCATTACGTGAAg               <  1:336879/251‑1 (MQ=35)                                                                                                                                                                                                                                             gggATCACTCCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCT‑GGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGC‑GAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTACGGCCCCGGGGGGATGTCATTACGTGAAGTCACTGc        <  2:287008/251‑1 (MQ=33)                                                                                                                                                                                                                                                   aCTCCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCT‑GGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGC‑GAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTACGGCCCCGGGGGGATGTCATTACGTGAAGTCACTGCATGGGc  <  1:136305/251‑1 (MQ=11)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                            GTCGTCGAAACACCGGTAGGCCTGAACGAAAGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCT‑GGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGC‑GAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTACGGCCCCGGGGGGATGTCATTACGTGAAGTCACTGCATGGGC  >  NC_000913/15143‑15635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.