Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,606,094	(G)8→9	noncoding (72/87 nt)	leuV ←	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,606,094	1	.	G	93.3%	84.8 / 0.6	30	noncoding (72/87 nt)	leuV	tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base . (0/2);  new base G (17/11);  total (17/13)
Fisher's exact test for biased strand distribution p-value = 1.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.21e-01

GATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAGTGGCGGATTGTCATTCTGATGGGATTAGTATTGACGCCGATGATGCTGTATCACAAACAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGG‑GG‑G‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATC‑TTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑G‑GGGGGGG‑A‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACT  >  NC_000913/4605857‑4606333                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     gATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAGTGGCGGATTGTCATTCTGATGGGATTAGTATTGACGCCGATGATGCTGTATCACCAACAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATGGGTGCGAtgggg‑gg‑gtgctttaaccccc                                                                                                                                                                                                                                          <  2:24450/251‑14 (MQ=255)             gATGTATGCGGATTTATCGTCAGATCAGTGGCGGATTGTCATTCTGATGGGATTAGTATTGACGCCGATGATGCTGTATCACAAACAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAATGAAGTAAGGATGATTGGTGCGAGGGGG‑GG‑GGACTTGAACCCCCACGTCCGTaa                                                                                                                                                                                                                                <  1:239441/249‑1 (MQ=255)                gTATGCGGATTTATCGTCAGATCAGTGGCGGATTGTCATTCTGATGGGATTAGTATTGACGCCGATGATGCTGTATCACAAACAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGG‑GG‑GGACTTGAACCCCCACGTCCGTAAGGac                                                                                                                                                                                                                            <  1:92248/250‑1 (MQ=255)                          ttATCGTCAGATCAGTGGCGGATTGTCATTCTGATGGGCTTAGTATTGACGCCGATGATGCTGTATCACAAACAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGG‑GG‑GGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTg                                                                                                                                                                                                                 >  2:327175/1‑251 (MQ=255)                          ttATCGTCAGATCAGTGGCGGATTGTCATTCTGATGGGATTAGTATTGACGCCGATGATGCTGTATCACAAACAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGG‑GG‑GGACTTGAACCCCCACGTCCGTAAGGACACTAAAAcccg                                                                                                                                                                                                                 >  2:365073/1‑249 (MQ=255)                            aTCGTCAGATCAGTGGCGGATTGTCATTCTGATGGGATTAGTATTGACGCCGATGATGCTGTATCACAAACAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGG‑GG‑GGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGa                                                                                                                                                                                                                <  2:316741/250‑1 (MQ=255)                               ggcAGATCAGTGGCGGATTGTCATTCTGATGGGATTAGTATTGACGCCGATGATGCTGTATCACAAACAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATGGGTGCGAGGGGG‑GG‑GGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCt                                                                                                                                                                                                            <  1:365073/249‑1 (MQ=255)                                       aGTGGCGGATTGTCCATATGGTGGGATTAGTATTGACGCCGATGCTGCTGTATCACAAACAGGTGCGGCATTACATTTTTCTACCATCGTGCCTGGCCCCTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCCGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTTGTGCGAGGGGG‑GG‑GGACTTGAACCCCCCAGTCCGTAAGGACACTAACACCTTAAGATCGCGCGTc                                                                                                                                                                                                    >  2:73340/1‑251 (MQ=255)                                          ggCGGATTGTCAATCTGATGGGATTAGTATTTACGCCGATGATGCTGTATCACAAACAGTGGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCAGGCTGATGATAATGAATTTGAATCAGGGAGGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAACTGGTGCGAGGGGG‑GGTG‑ACTTGAGCCCCCGCGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTAc                                                                                                                                                                                                 <  1:45958/251‑1 (MQ=39)                                                               gATTAGTATTGACGCCGATGATGCTGTATCACAAACAGTTGCGGCATTACATTTTGCTACCCTCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGG‑GG‑GGACTTGAACACCCACGTCCGTAAGGCCACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcac                                                                                                                                                                             >  2:249113/1‑250 (MQ=255)                                                                      aTTGACGCCGATGATGCGGTATCACAAACAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAAGCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGG‑GG‑GGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCTCCTTCGCACAGTCATC‑t                                                                                                                                                                    <  2:188644/251‑1 (MQ=255)                                                                         gACGCCGATGATGCTGTATCACAAACAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGG‑GG‑GGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATC‑TTAc                                                                                                                                                                 >  2:79794/1‑251 (MQ=255)                                                                                 tgatgCTGTATCACAAACAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGG‑GG‑GGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCa                                                                                                                                                                                               <  2:189628/214‑1 (MQ=255)                                                                                 tgatgCTGTATCACAAACAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGG‑GG‑GGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCa                                                                                                                                                                                               >  1:189628/1‑214 (MQ=255)                                                                                   atgCTGTATCACAAACAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGG‑GG‑GGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATC‑TTACTTTTTTTGat                                                                                                                                                       >  2:1813/1‑251 (MQ=255)                                                                                        gTATCACAAACAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGG‑GG‑GGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATC‑TTACTTTTTTTGATATCGc                                                                                                                                                  <  1:44095/251‑1 (MQ=255)                                                                                                  cAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGG‑GG‑GGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCg                                                                                                                                                                                <  2:148148/212‑1 (MQ=255)                                                                                                  cAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGG‑GG‑GGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCg                                                                                                                                                                                >  1:148148/1‑212 (MQ=255)                                                                                                       gCGGCATTCCATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCAACATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGTAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGTGG‑G‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATC‑TTACTTTTTTTGATATCGCCTCGTTTGGTGcta                                                                                                                                    <  2:287734/250‑3 (MQ=255)                                                                                                                       cTACCATCGTGACTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGG‑GG‑GGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAG‑CAACATTACTTTTTTTGATATACCCTCGTTTGGTGCGA‑GTGGGGTGG‑A‑CTTTAAc                                                                                                                >  2:236336/1‑251 (MQ=37)                                                                                                                                                       catGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGG‑GG‑GGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATC‑TTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑G‑GGGGGGG‑A‑CTTTAACCCCCCCGTCCGTAAGAACACTAACACCTGAAGc                                                                               >  1:135334/1‑251 (MQ=255)                                                                                                                                                          gCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGG‑GG‑GGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATC‑TTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑G‑GGGGGGG‑GGCTTGAACCCCCAc                                                                                                          >  1:323400/1‑222 (MQ=255)                                                                                                                                                          gCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGG‑GG‑GGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATC‑TTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑G‑GGGGGGG‑GGCTTGAACCCCCAc                                                                                                          <  2:323400/222‑1 (MQ=255)                                                                                                                                                            tgatgaTAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGG‑GG‑GGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCCCACAGTCTTC‑TTACTTTTTTTGATATCCCCTCGTGTGGTCCGATGCGGGGGGT‑C‑CTTATACTCCCACGTCCGGAAGAACACTGACACCTGAGGCTat                                                                            >  2:304726/1‑250 (MQ=25)                                                                                                                                                                      gAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGG‑GG‑GGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATC‑TTACTTTTTTTGATATCACCTCGTTTGGTGCGA‑G‑GGGGGGG‑A‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGCAGCTAGCGCGTCTACCa                                                                 >  1:218873/1‑250 (MQ=255)                                                                                                                                                                                  gggATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGG‑GG‑GGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACACTCCTC‑TTACTTTTATTGATTTCGCCTCGTTTGGGGCGG‑G‑GGGGGGGGA‑CTTTAAACCCCTCGGCGGTAAGAACACCAACACCAAAAGCTaacccggctaccccatcccccccct                                                     >  2:317426/1‑227 (MQ=18)                                                                                                                                                                                                         cAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGG‑GG‑GGACTTGAACCCCCACGTCCGTAAGGACACTAACCCCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTGCGCCCAGTCATC‑TTTCTTTTTTTGATATCGCCACGTTTGGTGCGA‑G‑GGGGGGGGA‑TTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGc                                          <  2:183229/239‑1 (MQ=25)                                                                                                                                                                                                         cAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGG‑GG‑GGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATC‑TTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑G‑GGGGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGc                                          >  1:183229/1‑239 (MQ=255)                                                                                                                                                                                                          aaGAAAGGAAGTAAAGATAATTGGTGGGAGGGGG‑GG‑GGACTTGAACCCCCCCGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATC‑TTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑G‑GGGGGGG‑GGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAAc                             <  1:79794/251‑1 (MQ=255)                                                                                                                                                                                                             aaaGGAAGTAAAGATAATTGGTGCGAGGGGG‑GG‑GGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATC‑TTACTTTTTTTGATATCTCCTCGTTTGGCGCGA‑G‑GGGGGGG‑A‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCCCCCAGTGCGAGTAATGTCATCGTgg                         >  1:104512/1‑251 (MQ=37)                                                                                                                                                                                                                             aTTGGTGCGAGGGGG‑GG‑GGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATC‑TTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑G‑GGGGGGG‑A‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATAAACGTGGTTTTTGGTGCGCgggg         >  1:7391/1‑251 (MQ=255)                                                                                                                                                                                                                                  cgaggggggg‑gg‑g‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTAATC‑TTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑G‑GGGGGGG‑A‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGGGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGAc   >  1:318275/6‑251 (MQ=255)                                                                                                                                                                                                                                   cgagggggg‑gg‑g‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATC‑TTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑G‑GGGGGGG‑A‑CTTGAACCCCCACGTCCGTAAGAACACTACCACCTGAAGCTAGCACGTCTACCAATCACGCCACCTACGCCCAGTGCGAGTAATATCAACGTGGTGTTTGGTTCGAGGGGAGGGACt  >  1:254060/5‑251 (MQ=37)                                                                                                                                                                                                                                    gagggggg‑gg‑g‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATC‑TTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑G‑GGGGGGG‑A‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATc                                >  2:195596/4‑220 (MQ=255)                                                                                                                                                                                                                                    gagggggg‑gg‑g‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATC‑TTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑G‑GGGGGGG‑A‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATc                                <  1:195596/217‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     GATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAGTGGCGGATTGTCATTCTGATGGGATTAGTATTGACGCCGATGATGCTGTATCACAAACAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGG‑GG‑G‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATC‑TTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑G‑GGGGGGG‑A‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACT  >  NC_000913/4605857‑4606333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑