Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,986,140	(T)6→7	coding (294/1230 nt)	yqeG →	putative transporter YqeG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,986,140	1	.	T	100.0%	52.2 / NA	16	F98? (TTT→TTN)	yqeG	putative transporter YqeG
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (7/9);  total (7/9)

GCAGGGGCTGTGGTCCTGTTTATTACTGCTCTGGTCGCCTGGCCTTTAACATATTGGCCACATAAAGCCTTGTGCCAGTTCATCCTCTCATCAAAAACATCAGCAGGTGAAGGGATAACGGGCGCGGTAACACACTACTATGGCAAGAAGATTGGTAATCTGATTACCACGCTGTACTTCATCGCCTTTTTT‑GTCGTCGTGTTGATATATGCAGTGGCAATTACCAACTCACTTACGGAACAGCTGGCAAAGCATATGGTTATTGATCTTCGCATCCGTATGTTGGTGAGTCTGGGTGTTGTATTAATTCTGAATCTCATTTTTCTGATGGGACGTCATGCCACTATTCGGGTAATGGGATTTTTGGTATTCCCATTGATTGC  >  NC_000913/2985949‑2986331                                                                                                                                                                                                 |                                                                                                                                                                                                gCAGGGGCTGTGGTCCTGTTTATTACTGCTCTGGTCGCCTGGCCTTTAACATATTGGCCACATAAAGCCTTGTGCCAGTTCATCCTCTCATCAAAAACATCAGCAGGTGAAGGGATAACGGGCGCGGTAACACACTACTATGGCAAGAAGATTGGTAATCTGATTACCACGCTGTACTTCATCGCCTTTTTTTGTCGTCGTGTTGATATATGCAGTGGCAATTACCAACTCACTTACGGAACAGCTGGCa                                                                                                                                        >  2:244280/1‑250 (MQ=255)           tgGTCCTGTTTATTACTGCTCTGGTCGCCTGGCCTTTAACATATTGGCCACATAAAGCCTTGTGCCAGTTCATCCTCTCATCAAAAACATCAGCAGGTGAAGGGATAACGGGCGCGGTAACACACTACTATGGCAAGAAGATTGGTAATCTGATTACCACGCTGTACTTCATCGCCTTTTTTTGTCGTCGTGTTGATATATGCAGTGGCAATTACCAACTCACTTACGGAACAGCTGGCAAAGCATATGGt                                                                                                                             >  2:368412/1‑251 (MQ=255)            ggTCCTGTTTATTACTGCTCTGGTCGCCTGGCCTTTAACATATTGGACACCTAAAGCATTGTGCCAGTTAATCCTCTCATCAAAAAAATCAGCAGGTGAAGGGATAACGGGCGCGGTAACACACTACTATGGCAAGAAGCTTGGTAATCTGATTACCACGCTGTACTTCATCGCCTTTTTTTGTCGTCGTGTTGATATATGCAGTGGCAATTACCAACTCACTTACGGAACAGCTGGCAAAGCATATGGtt                                                                                                                            >  1:149681/1‑251 (MQ=255)                            gCTCTGGTCGCCTGGCCTTTAACATATTGGCCACATAAAGCCTTGTGCCAGTTCATCCTCTCATCAAAAACATCAGCAGGTGAAGGGATAACGGGCGCGGTAACACACTACTATGGCAAGAAGATTGGTAATCTGATTACCACGCTGTACTTCATCGCCTTTTTTTGTCGTCGTGTTGATATATGCAGTGGCAATTACCAACTCACTTACGGAACAGCTGGCAAAGCATATGGTTATTGATCTTCGCATcc                                                                                                            <  1:210808/251‑1 (MQ=255)                              tctGGTCGCCTGGCCTTTAACATATTGGCCACATAAAGCCTTGTGCCAGTTCATCCTCTCATCAAAAACATCAGCAGGTGAAGGGATAACGGGCGCGGTAACACACTACTATGGCAAGAAGATTGGTAATCTGATTACCACGCTGTACTTCATCGCCTTTTTTTGTCGTCGTGTTGATATATGCAGTGGCAATTACCAACTCACTTACGGAACAGCTGGCAAAGCATATGGTTATTGATCTTCGCATCCGt                                                                                                          >  2:259709/1‑251 (MQ=255)                                  gTCGCCTGGCCTTTAACATATTGGCCACATAAAGCCTTGTGCCAGTTCATCCTCTCATCAAAAACATCAGCAGGTGAAGGGATAACGGGCGCGGTAACACACTACTATGGCAAGAAGATTGGTAATCTGATTACCACGCTGTACTTCATCGCCTTTTTTTGTCGTCGTGTTGATATATGCAGTGGCAATTACCAACTCACTTACGGAACAGCTGGCAAAGCATATGGTTATTGATCTTCGCATCCGTATGt                                                                                                      <  1:261000/251‑1 (MQ=255)                                             tttAACATATTGGCCACATAAAGCCTTGTGCCAGTTCATCCTCTCATCAAAAACATCAGCAGGTGAAGGGATAACGGGCGCGGTAACACACTACTATGGCAAGAAGATTGGTAATCTGATTACCACGCTGTACTTCATCGCCTTTTTTTGTCGTCGTGTTGATATATGCAGTGGCAATTACCAACTCACTTACGGAACAGCTGGCAAAGCATATGGTTATTGATCTTCGCATCCGTATGTTGGTGAGTCTg                                                                                           >  1:53587/1‑251 (MQ=255)                                                       tGGCCACATAAAGCCTTGTGCCAGTTCATCCTCTCATCAAAAACATCAGCAGGTGAAGGGATAACGGGCGCGGTAACACACTACTATGGCAAGAAGATTGGTAATCTGATTACCACGCTGTACTTCATCGCCTTTTTTTGTCGTCGTGTTGATATATGCAGTGGCAATTACCAACTCACTTACGGAACAGCTGGCAAAGCATATGGTTATTGATCTTCGCATCCGTATGTTGGTGAGTCTGGGTGTTGTa                                                                                  <  2:103960/250‑1 (MQ=255)                                                        ggCCACATAAAGCCTTGTGCCAGTTCATCCTCTCATCAAAAACATCAGCAGGTGAAGGGATAACGGGCGCGGTAACACACTACTATGGCAAGAAGATTGGTAATCTGATTACCACGCTGTACTTCATCGCCTTTTTTTGTCGTCGTGTTGATATATGCAGTGGCAATTACCAACTCACTTACGGAACAGCTGGCAAAGCATATGGTTATTGATCTTCGCATCCGTATGTTGGTGAGTCTGGGTGTTGTAt                                                                                 <  2:91993/250‑1 (MQ=255)                                                               tAAAGCCTTGTGCCAGTTCATCCTCTCATCAAAAACATCAGCAGGTGAAGGGATAACGGGCGCGGTAACACACTACTATGGCAAGAAGATTGGTAATCTGATTACCACGCTGTACTTCATCGCCTTTTTTTGTCGTCGTGTTGATATATGCAGTGGCAATTACCAACTCACTTACGGAACAGCTGGCAAAGCATATGGTTATTGATCTTCGCATCCGTATGTTGGTGAGTCTGGGTGTTGTATTAATTCTg                                                                         >  2:185458/1‑251 (MQ=255)                                                                                             aaaaaCATCAGCAGGTGAAGGGATAACGGGCGCGGTAACACACTACTATGGCAAGAAGATTGGTAATCTGATTACCACGCTGTACTTCATCGCCTTTTTTTGTCGTCGTGTTGATATATGCAGTGGCAATTACCAACTCACTTACGGAACAGCTGGCAAAGCATATGGTTATTGATCTTCGCATCCGTATGTTGGTGAGTCTGGGTGTTGTATTAATTCTGAATCTCATTTTTCTGATGGGACGTCATGcc                                           <  1:244280/251‑1 (MQ=255)                                                                                                aaCATCAGCAGGTGAAGGGATAACGGGCGCGGTAACACACTACTATGGCAAGAAGATTGGTAATCTGATTACCACGCTGTACTTCATCGCCTTTTTTTGTCGTCGTGTTGATATATGCAGTGGCAATTACCAACTCACTTACGGAACAGCTGGCAAAGCATATGGTTATTGATCTTCGCATCCGTATGTTGGTGAGTCTGGGTGTTGTATTAATTCTGAATCTCATTTTTCTGATGGGACGTCATGCCACt                                        <  1:185458/251‑1 (MQ=255)                                                                                                         agGTGAAGGGATAACGGGCGCGGTAACACACTACTATGGCAAGAAGATTGGTAATCTGATTACCACGCTGTACTTCATCGCCTTTTTTTGTCGTCGTGTTGATATATGCAGTGGCAATTACCAACTCACTTACGGAACAGCTGGCAAAGCATATGGTTATTGATCTTCGCATCCGTATGTTGGTGAGTCTGGGTGTTGTATTAATTCTGAATCTCATTTTTCTGATGGGACGTCAGGCCACTATTCGGGTa                               <  2:368880/251‑1 (MQ=255)                                                                                                                                     cacTACTATGGCAAGAAGATTGGTAATCTGATTACCACGCTGTACTTCATCGCCTTTTTTTGTCGTCGTGTTGATATATGCAGTGGCAATTACCAACTCACTTACGGAACAGCTGGCAAAGCATATGGTTATTGATCTTCGCATCCGTATGTTGGTGAGTCTGGGTGTTGTATTAATTCTGAATCTCATTTTTCTGATGGGACGTCATGCCACTATTCGGGTAATGGGATTTTTGGTATTCCCattgattg   <  2:140917/251‑1 (MQ=255)                                                                                                                                     cacTACTATGGCAAGAAGATTGGTAATCTGATTACCACGCTGTACTTCATCGCCTTTTTTTGTCGTCGTGTTGATATATGCAGTGGCAATTACCAACTCACTTACGGAACAGCTGGCAAAGCATATGGTTATTGATCTTCGCATCCGTATGTTGGTGAGTCTGGGTGTTGTATTAATTCTGAATCTCATTTTTCTGATGGGACGTCATGCCACTATTCGGGTAATGGGATTTTTGGTATTCCCattgattg   >  2:37577/1‑251 (MQ=255)                                                                                                                                      cctactaTGGCAAGAAGATTGGTAATCTGATTACCACGCTGTCCTTCATCGCCTTTTTTTGTCGTCGTGTTGATATATGCAGTGGCAATTACCAACTCACTTACGGAACAGCTTGCAAAGCAGATGGTTATTGATCTTCGCATCCGTATGTTGGTGAGTCTGGGTGTTGTATTAATTCTGAATCGCATTGTGCTGATGGGACGACATGCCACTATTCGTGTAATGCTATTTTTGGTATTCCCATTGAATGc  <  2:313111/250‑1 (MQ=255)                                                                                                                                                                                                 |                                                                                                                                                                                                GCAGGGGCTGTGGTCCTGTTTATTACTGCTCTGGTCGCCTGGCCTTTAACATATTGGCCACATAAAGCCTTGTGCCAGTTCATCCTCTCATCAAAAACATCAGCAGGTGAAGGGATAACGGGCGCGGTAACACACTACTATGGCAAGAAGATTGGTAATCTGATTACCACGCTGTACTTCATCGCCTTTTTT‑GTCGTCGTGTTGATATATGCAGTGGCAATTACCAACTCACTTACGGAACAGCTGGCAAAGCATATGGTTATTGATCTTCGCATCCGTATGTTGGTGAGTCTGGGTGTTGTATTAATTCTGAATCTCATTTTTCTGATGGGACGTCATGCCACTATTCGGGTAATGGGATTTTTGGTATTCCCATTGATTGC  >  NC_000913/2985949‑2986331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑