Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,774,809	(A)7→8	coding (1492/1704 nt)	yfjW →	uncharacterized protein YfjW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,774,809	1	.	A	100.0%	16.5 / NA	6	I498? (ATC→NTC)	yfjW	uncharacterized protein YfjW
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (2/4);  total (2/4)

TGATATTATTAATTACAACAATAGCCCTTCCGTACTACTCAAGACTGATTTTGCGTTCTATAAAAAAACATACCAGAAGCTTGATAAAATATATGATGACATAAGCAATGGAAAATTATCTTCACTGAGAGCAACAGGTATCTCCCAGTTTAGTATCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTATGGACTAGTTAATAAAAGCAAAAAAA‑TCAAAATATATGGCACAGCAGATCTTGTTTTTGTTGATAACAAGATAATGAACCTTCGTAAAATAACTTATTTGCAATCTAAGCTAGAAATTTTTGGTTCTTCTATTATGGATATATTAAAGTATATATTTGGTTTAGGTCTGCTAGCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTT  >  NC_000913/2774565‑2775059                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                           tgatATTATTAATTACAACAATAGCCCTTCCGTACTACTCAAGACTGATTTTGCGTTCTATAAAAAAACATACCAGAAGCTTGATAAAATATATGATGACATAAGCAATGGAAAATTATCTTCACTGAGAGCAACAGGTATCTCCCAGTTTAGTATCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTATGGACTAGTTAATAAAAGCaaaaaaaatcaa                                                                                                                                                                                                                                                        >  1:63298/1‑245 (MQ=255)          taattaCAACAATAGCCCTTCCGTACTACTCAAGACTGATTTTGCGGTCTATAAAAAAACATACCAGAAGCGTGATAAAATATATGATGACATAAGCAATGGAAAATTATCTTCTCTGAGAGCAACAGGTATCTCCCAGTTTAGTATCAACGGGAAACATTTGTCGTTAAGGCCAGAATCTGAGATTATTATTTCTGAAGTGAGTTTGTATGGACTAGTTAATAAAAGCAAAAAAAATGAAAATATATgg                                                                                                                                                                                                                                               <  2:274841/250‑1 (MQ=255)                                                                                                                                                           aTCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTATGGACTAGTTAATAAAAGCAAAAAAAATCAAAATATATGGCACAGCAGATCTTGTTTTTGTTGATAACAAGATAATGAACCTTCGTAAAATAACTTATTTGCAATCTAAGCTAGAAATTTTTGGTTCTTCTATTATGGATATATTAAAGTATATATTTGGTTTAGGTCTGCTAGCAATTTCtata                                                                                              <  2:299757/250‑1 (MQ=255)                                                                                                                                                                      aCATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTATGGACTAGTTAATAAAAGCAAAAAAAATCAAAATATATGGCACAGCAGATCTTGTTTTTGTTGATAACAAGATAATGAACCTTCGTAAAATAACTTATTTGc                                                                                                                                                                                 >  1:308019/1‑156 (MQ=255)                                                                                                                                                                      aCATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTATGGACTAGTTAATAAAAGCAAAAAAAATCAAAATATATGGCACAGCAGATCTTGTTTTTGTTGATAACAAGATAATGAACCTTCGTAAAATAACTTATTTGc                                                                                                                                                                                 <  2:308019/156‑1 (MQ=255)                                                                                                                                                                                                             tGAAGGGAGTTTGTATGGACTAGTTACTAAAAGCAAAAAAAATCAAAATATATGGCACAGCAGATCTTGTTTTTGTTGATAACAAGATAATGAACCTTCGTAAAATAACTTATTTGCAATCTaa                                                                                                                                                                          >  1:329486/1‑124 (MQ=255)                                                                                                                                                                                                             tGAAGGGAGTTTGTATGGACTAGTTAATAAAAGCAAAAAAAATCAAAATATATGGCACAGCAGATCTTGTTTTTGTTGATAACAAGATAATGAACCTTCGTAAAATAACTTATTTGCAATCTaa                                                                                                                                                                          <  2:329486/124‑1 (MQ=255)                                                                                                                                                                                                                                                     a‑TCAAAATATATGGCACAGCAGATCTTGTTTTTGTTGATAACAAGATAATGAACCTTCGTAAAATAACTTATTTGCAATCTAAGCTAGAAATTTTTGGTTCTTCTATTATGGATATATTAAAGTATATATTTGGTTTAGGTCTGCTAGCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACtattt  >  2:22132/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                           TGATATTATTAATTACAACAATAGCCCTTCCGTACTACTCAAGACTGATTTTGCGTTCTATAAAAAAACATACCAGAAGCTTGATAAAATATATGATGACATAAGCAATGGAAAATTATCTTCACTGAGAGCAACAGGTATCTCCCAGTTTAGTATCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTATGGACTAGTTAATAAAAGCAAAAAAA‑TCAAAATATATGGCACAGCAGATCTTGTTTTTGTTGATAACAAGATAATGAACCTTCGTAAAATAACTTATTTGCAATCTAAGCTAGAAATTTTTGGTTCTTCTATTATGGATATATTAAAGTATATATTTGGTTTAGGTCTGCTAGCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTT  >  NC_000913/2774565‑2775059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑