Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,530,203 (C)5→6 intergenic (‑27/+44) ligA ← / ← zipA DNA ligase/cell division protein ZipA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,530,2031.C95.1% 133.0 / ‑0.7 41intergenic (‑27/+44)ligA/zipADNA ligase/cell division protein ZipA
Reads supporting (aligned to +/- strand):  ref base . (1/1);  new base C (17/22);  total (18/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCTGGCTGAAAGCCGCCAGCGGCGCAGCGCCTACACGTTGAGTAGGCGAATCAGGCGTAATCAGTTCTGGATGTTTGGTTTCCAGCTCGCGCAGTTCGCGCATCAGCCTGTCGTATTCAGCGTCGGGAATTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAAC‑CCCC‑GACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCACGACACCGCCCACTTCATCGGCAATATGCTGCGCAGATTGCAGCATCAGCTTGAAGTTCTGCAGCTCGTCACCGTAAGACGGTACCTGCATAAAGATAGTGACACCC  >  NC_000913/2529959‑2530442
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                               
aTCTGGCTGAAAGCCGCCAGCGGCGCAGCGCCTACACGTTGAGTAGGCGAATCAGGCGTAATCAGTTCTGGATGTTTGGTTTCCAGCTCGCGCAGTTCGCGCATCAGCCTGTCGTATTCAGCGTCGGGAATTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAAc‑cccccgaca                                                                                                                                                                                                                                             >  2:59514/1‑245 (MQ=255)
        gAAAGCCGCCAGCGGCGCAGCGCCTACACGTTGAGTAGGCGAATCAGGCGTAATCAGTTCTGGATGTTTGGTTTCCAGCTCGCGCAGTTCGCGCATCAGCCTGTCGTATTCAGCGTCGGGAATTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAAC‑CCCCCGACAAGCGGGGGt                                                                                                                                                                                                                                    <  2:252998/251‑1 (MQ=255)
          aaGCCGCCAGCGGCGCAGCGCCTACACGTTGAGTAGGCGCATAAGGCGTAATCAGTTCTGGATGTTTGGTTTCCAGCTCGCGCAGTTCGCGCATCAGCCTGTCGTATTCAGCGTCGGGAATTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAAC‑CCCCCGACAAGCGGGGGTTc                                                                                                                                                                                                                                  >  1:234473/1‑251 (MQ=255)
            gccgccAGCGGCGCAGCGCCTACACGTTGAGTAGGCGAATCAGGCGTAATCAGGTCTGGATGTTTGGTTTCCAGCTCGCGCAGTTCGCGCATCAGCCTGTCGTATTCAGCGTCGGGAATTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACACCCC‑GACAAGCTGGGGTTCg                                                                                                                                                                                                                                 >  1:202010/1‑250 (MQ=255)
              cgccAGCGGCGCAGCGCCTACACGTTGAGTAGGCGAATCAGGCGTAATCAGTTCTGGATGTTTGGTTTCCAGCTCGCGCAGTTCGCGCATCAGCCTGTCGTATTCAGCGTCGGGAATTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAAC‑CCCCCGACAAGCGGGGGTTCGAag                                                                                                                                                                                                                              <  1:201404/251‑1 (MQ=255)
                                    cGTTGAGTAGGCGAATCAGGCGTAATCAGTTCTGGATGTTTGGTTTCCAGCTCGCGCAGTTCGCGCATCAGCCTGTCGTATTCAGCGTCGGGAATTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAAC‑CCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAgtg                                                                                                                                                                                                        <  1:305227/251‑1 (MQ=255)
                                     gTTGAGTAGGCGAATCAGGCGTAATCAGTTCTGGATGTTTGGTTTCCAGCTCGCGCAGTTCGCGCATCAGCCTGTCGTAATCAGCGTCGGGAATTTCCGGCGCATCCATCACATGATAAAGATATGCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAAC‑CCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAgtgt                                                                                                                                                                                                       <  2:293173/251‑1 (MQ=255)
                                             ggCGAATCAGGCGTAATCAGTTCTGGATGTTTGGTTTCCAGCTCGCGCAGTTCGCGCATCAGCCTGTCGTATTCAGCGTCGGGAATTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAAC‑CCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCg                                                                                                                                                                                               >  1:221681/1‑251 (MQ=255)
                                                  aTCAGGCGTAATCAGTTCTGGATGTTTGGTTTCCAGCTCGCGCAGTTCGCGCATCAGCCTGTCGTATTCAGCGTCGGGAATTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAAC‑CCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGc                                                                                                                                                                                          <  1:59514/251‑1 (MQ=255)
                                                     aGGCGTAATCAGTTCTGGATGTTTGGTTTCCAGCTCGCGCAGTTCGCGCATCAGCCTGTCGTATTCAGCGTCGGGAATTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAAC‑CCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTc                                                                                                                                                                                       >  1:301420/1‑251 (MQ=255)
                                                            aTCAGTTCTGGATGTTTGGTTTCCAGCTCGCGCAGTTCGCGCATCAGCCTGTCGTATTCAGCGTCGGGAATTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAAC‑CCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACt                                                                                                                                                                                >  1:192962/1‑251 (MQ=255)
                                                                     ggATGTTTGGTTTCCAGCTCGCGCAGTTCGCGCATCAGCCTGTCGTATTCAGCGTCGGGAATTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAAC‑CCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGgatg                                                                                                                                                                       <  1:218862/251‑1 (MQ=255)
                                                                      gATGTTTGGTTTCCAGCTCGCGCAGTTCGCGCATCAGCCTGTCGTATTCAGCGTCGGGAATTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAAC‑CCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGgatga                                                                                                                                                                      <  1:5412/251‑1 (MQ=255)
                                                                                    aGCTCGCGCAGTTCGCGCATCAGCCTGTCGTATTCAGCGTCGGGAATTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAAC‑CCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTcg                                                                                                                                                        >  1:134685/1‑251 (MQ=255)
                                                                                                    gcATCAGCCTGTCGTATTCAGCGTCGGGAATTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAAC‑CCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCgg                                                                                                                                          >  1:241143/1‑249 (MQ=255)
                                                                                                                cGTATTCAGCGTCGGGAATTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTACATATCGCACCATCAATGCTAAAAAC‑CCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGgc                                                                                                                            >  2:285040/1‑251 (MQ=255)
                                                                                                                   aTTCAGCGTCGGGAATTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAAC‑CCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTGCTGCGGAGTCATCATACGGCGCt                                                                                                                         <  1:101095/251‑1 (MQ=255)
                                                                                                                     tCAGCGTCGGGAATTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAAC‑CCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTg                                                                                                                        >  1:93849/1‑250 (MQ=255)
                                                                                                                            cGGGAATTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAAC‑CCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCaa                                                                                                                <  2:340572/251‑1 (MQ=255)
                                                                                                                                aaTTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAAC‑CCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCAc                                                                                                            <  1:55147/251‑1 (MQ=255)
                                                                                                                                 aTTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAAC‑CCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCACg                                                                                                           >  1:246931/1‑251 (MQ=255)
                                                                                                                                              ccatcACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAAC‑CCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCACGACACCGCCCACt                                                                                               <  2:183584/250‑1 (MQ=255)
                                                                                                                                                atcACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAAC‑CCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCACGACACCGCCCACTTc                                                                                             >  1:252896/1‑250 (MQ=255)
                                                                                                                                                        aTAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAAC‑CCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCACGACACCGCCCACTTCATCGGCAat                                                                                    <  1:10651/251‑1 (MQ=255)
                                                                                                                                                              atatTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAAC‑CCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCACGACACCGCCCACTTCATCGGCAATAtgctg                                                                              <  2:89990/251‑1 (MQ=255)
                                                                                                                                                                   cATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAAC‑CCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCACGACACCGCCCACTTCATCGGCAATATGCTGCGCAg                                                                         <  1:46155/251‑1 (MQ=255)
                                                                                                                                                                       atgGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAAC‑CCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCACGACACCGCCCACTTCATCGGCAATATGCTGCGCAGATTg                                                                     >  1:347087/1‑251 (MQ=255)
                                                                                                                                                                       atgGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAAC‑CCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCACGACACCGCCCACTTCATCGGCAATATGCTGCGCAGATTg                                                                     <  1:352535/251‑1 (MQ=255)
                                                                                                                                                                        tgGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAAC‑CCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCACGACACCGCCCACTTCATCGGCAATATGCTGCGCAGTTTgc                                                                    >  1:351264/1‑251 (MQ=255)
                                                                                                                                                                        tgGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAAC‑CCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTGGACTTCGCGGTTGATTCCCTGGTACTCGCGCAATTTCTGCGGGGTCATCATACGGCGCTGATCGTAAAGAACGAACCCCCCCACTTCACCGGCATTCTCTTGCGCAGAttcc                                                                    >  2:322796/1‑249 (MQ=255)
                                                                                                                                                                         ggCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAAC‑CCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCACGACACCGCCCACTTCATCGGCAATATGCTGCGCAGATTgca                                                                   <  2:268397/251‑1 (MQ=255)
                                                                                                                                                                             aaGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAAC‑CCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCACGACACCGCCCACTTCATCGGCAAGATGCTGCGCAGATGGCAGCAt                                                               <  2:24912/251‑1 (MQ=255)
                                                                                                                                                                                 gtcgtTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAAC‑CCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGa                                                                                                                                         <  1:153244/173‑1 (MQ=255)
                                                                                                                                                                                 gtcgtTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAAC‑CCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGa                                                                                                                                         >  2:153244/1‑173 (MQ=255)
                                                                                                                                                                                      tCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAAC‑CCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCACGACACCGCCCACTTCATCGGCAATATGCTGCGCAGATTGCAGCATCAGCTTGa                                                       >  2:14347/1‑250 (MQ=255)
                                                                                                                                                                                        gCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAAC‑CCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCACGACACCGCCCACTTCATCGGCAATATGCTGCGCAGATTGCAGCATCAGCTTGAAGt                                                    >  1:80284/1‑251 (MQ=255)
                                                                                                                                                                                          aGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAAC‑CCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCACGACACCGCCCACTTCATCGGCAATATGCTGCGCAGATTGCAGCATCAGCTTGAAGTTc                                                  <  1:214420/251‑1 (MQ=255)
                                                                                                                                                                                           gTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAAC‑CCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCACGACACCGCCCACTTCATCGGCAATATGCTGCGCAGATTGCAGCATCAGCTTGAAGTTCt                                                 <  1:177624/251‑1 (MQ=255)
                                                                                                                                                                                           gTTCTGTCAGTTGTTGTTCGATTGAGTCCATATCGCACCATCAATGCTAAAAAC‑CCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGTCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCACGACACCGCCCACTTCATCGGCAATATGCTGCGCAGATTGCAGCATCAGCTTGAAGTTCt                                                 <  2:5272/251‑1 (MQ=255)
                                                                                                                                                                                                   aGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAAC‑CCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCACGACACCGCCCACTTCATCGGCAATATGCTGCGCAGATTGCAGCATCAGCTTGAAGTTCTGCAGCTCg                                         >  1:296424/1‑251 (MQ=255)
                                                                                                                                                                                                                                        gCTAAAAAC‑CCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCACGACACCGCCCACTTCATCGGCAATATGCTGCGCAGATTGCAGCATCAGCTTGAAGTTCTGCAGCTCGTCACCGTAAGACGGTACCTGCATAAAGATAGTGacac    <  1:78865/251‑1 (MQ=255)
                                                                                                                                                                                                                                          aaaaacC‑CCCC‑GACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCACGACACCGCCCACTTCATCGGCAATATGCTGCGCAGAGTGCAGCATCAGCTTGAAGTTCTGCAGCTCGTCACCGTAAGACGGTACCTGCCTAAAGATAGTGACAccc  <  2:221681/249‑1 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                               
ATCTGGCTGAAAGCCGCCAGCGGCGCAGCGCCTACACGTTGAGTAGGCGAATCAGGCGTAATCAGTTCTGGATGTTTGGTTTCCAGCTCGCGCAGTTCGCGCATCAGCCTGTCGTATTCAGCGTCGGGAATTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAAC‑CCCC‑GACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCACGACACCGCCCACTTCATCGGCAATATGCTGCGCAGATTGCAGCATCAGCTTGAAGTTCTGCAGCTCGTCACCGTAAGACGGTACCTGCATAAAGATAGTGACACCC  >  NC_000913/2529959‑2530442

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑