Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,674,655	(C)6→7	coding (743/1035 nt)	tqsA →	autoinducer 2 exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,674,655	1	.	C	96.8%	94.1 / ‑2.6	31	coding (743/1035 nt)	tqsA	autoinducer 2 exporter
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base C (16/14);  total (16/15)
Fisher's exact test for biased strand distribution p-value = 4.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.21e-01

CAATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCC‑CCCC‑TATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGA  >  NC_000913/1674411‑1674902                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                        caATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCC‑CCCCCTATCg                                                                                                                                                                                                                                                    >  2:257247/1‑251 (MQ=255)          ggAAACTTTCAGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCACTCC‑CCCCCTATCGCTCAGGTAc                                                                                                                                                                                                                                           >  2:125043/1‑251 (MQ=255)          ggAAAATTTCAGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCC‑CCCCCTATCGCTCAGGTAc                                                                                                                                                                                                                                           >  1:37539/1‑251 (MQ=255)           gAAAATTTCAGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCC‑CCCCCTATCGCTCAGGTACt                                                                                                                                                                                                                                          >  1:147657/1‑251 (MQ=255)                tttCAGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCAGCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCC‑CCCCCTATCGCTCAGGTACTGgtg                                                                                                                                                                                                                                      <  2:315942/250‑1 (MQ=255)                     gCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATTACATCCCTAATATTGGTTCAGTCCTCGCGGCAATCC‑CCCCCTATCGCTCAGGTACTGGTGTTTAAt                                                                                                                                                                                                                                <  1:35081/251‑1 (MQ=255)                         atgaagGCGCGTCCGGGTGAAGGGATGGCGGCGATTCAACGTGCGATTTACAGTGTTTCTCATTATCTGGTGCTGAAAACAGGCATCAGCATCATCTCCGGCCTGGTCGCCTGGGCGATGCCCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCC‑CCCCCTATCGCTCAGGTACTGGTGTTTAATGGCt                                                                                                                                                                                                                            <  2:14190/251‑1 (MQ=255)                            atgGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCC‑CCCCCTATCGCTCAGGTACTGGTGTGTAATGGCTTCt                                                                                                                                                                                                                         <  1:329606/251‑1 (MQ=255)                              ggCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCC‑CCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTAc                                                                                                                                                                                                                       >  2:316231/1‑251 (MQ=255)                              ggCGCGTCCGGTGGAAGGGATGGTGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTGGCCTGGGCGATGCTCGCCGCACTCGATGGTCGCTTCTCTTTTGTCTGGGGATTGCTGGCCTTTGCGCGTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCC‑CCCCCTATCGCTCAGTTACTGGTGTTTAATGGCTTCTAc                                                                                                                                                                                                                       <  2:222189/251‑1 (MQ=255)                                     ccGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCC‑CCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCg                                                                                                                                                                                                                 <  1:196499/250‑1 (MQ=255)                                       ggTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCC‑CCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTtg                                                                                                                                                                                                              >  1:256421/1‑251 (MQ=255)                                                        cgATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCC‑CCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGat                                                                                                                                                                                              >  1:292522/1‑250 (MQ=255)                                                         gATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCC‑CCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGCAGCGTTGCTGGTGCTGGCGGGattt                                                                                                                                                                                            >  1:222255/1‑249 (MQ=255)                                                           ttCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCC‑CCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATTTct                                                                                                                                                                                          >  1:42447/1‑251 (MQ=255)                                                            tCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCC‑CCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATctg                                                                                                                                                                                         >  1:318571/1‑251 (MQ=255)                                                                     gATTGACTGTGCATCTCATTATCTGGTTCTGAAACCAGCCATCAGCATCATCACCGGCCTCTTAGCCTGGGCGATGCTCGCCTCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTTGCCTTTGCGCTTAATTACATCCCGAATTTTGGTTCAGTCCTCGCGTCAATCC‑CCCCCTTTCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAACCTTtgctggtttttgcttgtttttttcttattttt                                                                                                                                                                                >  2:45589/1‑226 (MQ=255)                                                                      aTTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCC‑CCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATctgctggattatt                                                                                                                                                                               >  2:290062/1‑244 (MQ=255)                                                                                             ggTGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCC‑CCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCt                                                                                                                                                            <  1:95050/247‑1 (MQ=255)                                                                                                  tGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCC‑CCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCcgcg                                                                                                                                                   >  1:202190/1‑251 (MQ=255)                                                                                                   gAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCC‑CCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCcgcg                                                                                                                                                   <  2:202525/250‑1 (MQ=255)                                                                                                                     catcaCCGGCCTTGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCC‑CCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGc                                                                                                                                  <  1:274152/249‑1 (MQ=255)                                                                                                                      atcaCCGGCCTGTTCGCCTGGGCGATGCTTGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATTACAGCCCGAATATTGGTTCAGGCCTCGCGGCAATCCGCCCC‑TATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATGTCCTGGAGCCGGGTATCATTGGGCggggcctga                                                                                                                               <  2:50785/251‑9 (MQ=255)                                                                                                                                                             gATGTTCGACTCGCTTTTGTCTGCGGATTGCTGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCC‑CCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTTATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGGATTTTTGTCGTTGTtttttt                                                                                        >  2:23454/1‑251 (MQ=255)                                                                                                                                                                                                   tGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCC‑CCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTc                                                   <  2:20518/250‑1 (MQ=255)                                                                                                                                                                                                          aaTTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCC‑CCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTTATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGGTGGTTGTTAGGTCCGGTGGGTATGCTGCTTTCCGTGCCGt                                           >  1:157868/1‑251 (MQ=255)                                                                                                                                                                                                            ttACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCC‑CCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTTGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTg                                         >  1:23645/1‑251 (MQ=255)                                                                                                                                                                                                                  cccGAATATTGGTTCAGTCCTCGCGGCAATCC‑CCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAat                                    <  1:369106/250‑1 (MQ=255)                                                                                                                                                                                                                         atTGGTTCAGTCCTCGCGGCAATCC‑CCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCa                            <  1:349346/251‑1 (MQ=255)                                                                                                                                                                                                                                         cgGCAATCC‑CCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGaaca            <  2:318571/251‑1 (MQ=255)                                                                                                                                                                                                                                          ggCAATCC‑CCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGATGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGTTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTTGTGTTATTTTTGTCGTTGATTTTTTGGGGATTTTTGTTAGTACCGGTGGGTATGCTGCTGTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTTaacaa           <  1:243695/251‑1 (MQ=255)                                                                                                                                                                                                                                                   cccc‑TATCGCTCAGGTAATGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGa  <  2:11852/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                        CAATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCC‑CCCC‑TATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGA  >  NC_000913/1674411‑1674902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑